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Gene information
General information
TC003502 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6055520..6057116
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003502
    iBeetle screen iB_09759

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber mostlynot present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003502 RA
      MSDQEMDVV DDNESENEE AMQEENEGN TEKPSEVYL 
      PTKPLEEGE ELVCDQSAY VMLHQAQTG APCLSFDII 
      KDSLGESRD TYPLTAYIV AGTQAPQTH VNNVIVMKL 
      SNLHKTNKG EDDDDDDDE ESDDDEVSK NPKMAGALI 
      KHQGCVNRI RSTVINNKI IAASWSELG RVNLWDLSQ 
      QLEAVDNDQ LLSRYNKEN KANSVKPLF TFTGHQQEG 
      FAMDWCSTN VGVLATGDC KRDIHIWKP ASGASWQVD 
      QRPLVGHTN SVEDLQWSP NERSVIASC SVDKSIRIW 
      DTRAQPSKA CMLTAENAH ESDVNVISW NKNEPFIVS 
      GGDDGFLHI WDLRRFQQK TPVATFKHH TEPVTTVEW 
      HPTDSAVFI SGGSDNQVA LWDLSVEKD DESGSEEVD 
      GIPPQLLFI HQGQNNIKE LHWHPQLPG VIISTAESG 
      FNIFRTISV
    CDS sequence
    • >TC003502 RA
      atgtcggac caagaaatg gacgtagtt gatgacaac 
      gagtcggaa aacgaagaa gcaatgcag gaggaaaat 
      gaagggaac acagaaaag ccatctgaa gtgtacctt 
      cccaccaaa ccactagaa gagggtgaa gaattggtg 
      tgtgaccag tccgcctat gttatgtta catcaggca 
      caaacaggt gccccgtgt cttagtttc gacatcata 
      aaagacagt ctgggggaa agtagagac acttacccg 
      ctaactgca tacattgtg gctggtact caagcccca 
      caaactcac gttaacaat gtgatagta atgaaatta 
      tcaaacctt cacaaaaca aataaaggc gaagatgac 
      gatgatgat gacgatgag gagtcagac gatgacgag 
      gtatcaaaa aatcccaaa atggcaggg gcccttatt 
      aaacatcaa ggttgtgtt aaccgaata agaagcacg 
      gtcataaat aacaaaata atcgcagcc tcttggagt 
      gagcttgga agagtgaat ttatgggat ctctcccag 
      cagttagag gcggtcgat aacgaccag ttgttatca 
      cgatataat aaggaaaat aaagctaat tcggtcaaa 
      ccgttgttc acgtttacg ggccaccag caggaaggt 
      tttgcaatg gattggtgc tccacaaat gtcggagtt 
      ttggcaaca ggggactgc aaaagagat atccacatt 
      tggaagcca gcaagtggg gcctcctgg caagtcgac 
      cagcggcct ctagttgga cacactaat tccgttgag 
      gacttgcaa tggtcaccg aatgagaga agtgttatt 
      gcttcatgc tcagttgat aaaagtatc agaatttgg 
      gacacaagg gcacagccg agcaaggcg tgcatgctg 
      acagctgaa aatgcacac gagagtgat gttaatgtt 
      ataagttgg aataaaaac gaacccttt attgtcagt 
      ggtggagac gatggattt ttacacatt tgggatttg 
      cggaggttt caacaaaaa acaccagtc gccacattt 
      aagcatcac acagagcca gtgactacc gttgaatgg 
      catccaact gattcagct gtgtttatc tcaggggga 
      agtgataat caagtggca ttatgggat ttgtctgtg 
      gaaaaggat gatgagagt ggtagtgaa gaagttgat 
      ggaattccg ccccaactt ttgttcatc catcaagga 
      caaaataat ataaaggaa ttgcactgg catccacaa 
      ctgccagga gtgataatc agtacggcg gagagtggt 
      tttaatata ttcagaact atcagcgtt taa
    mRNA sequence
    • >TC003502 RA
      ctggttgtc aaattttcg gcatttgag aaacacgtg 
      tgttttgtg ttggattta aatatcact ggaattatt 
      tatttgaaa cgatgtcgg accaagaaa tggacgtag 
      ttgatgaca acgagtcgg aaaacgaag aagcaatgc 
      aggaggaaa atgaaggga acacagaaa agccatctg 
      aagtgtacc ttcccacca aaccactag aagagggtg 
      aagaattgg tgtgtgacc agtccgcct atgttatgt 
      tacatcagg cacaaacag gtgccccgt gtcttagtt 
      tcgacatca taaaagaca gtctggggg aaagtagag 
      acacttacc cgctaactg catacattg tggctggta 
      ctcaagccc cacaaactc acgttaaca atgtgatag 
      taatgaaat tatcaaacc ttcacaaaa caaataaag 
      gcgaagatg acgatgatg atgacgatg aggagtcag 
      acgatgacg aggtatcaa aaaatccca aaatggcag 
      gggccctta ttaaacatc aaggttgtg ttaaccgaa 
      taagaagca cggtcataa ataacaaaa taatcgcag 
      cctcttgga gtgagcttg gaagagtga atttatggg 
      atctctccc agcagttag aggcggtcg ataacgacc 
      agttgttat cacgatata ataaggaaa ataaagcta 
      attcggtca aaccgttgt tcacgttta cgggccacc 
      agcaggaag gttttgcaa tggattggt gctccacaa 
      atgtcggag ttttggcaa caggggact gcaaaagag 
      atatccaca tttggaagc cagcaagtg gggcctcct 
      ggcaagtcg accagcggc ctctagttg gacacacta 
      attccgttg aggacttgc aatggtcac cgaatgaga 
      gaagtgtta ttgcttcat gctcagttg ataaaagta 
      tcagaattt gggacacaa gggcacagc cgagcaagg 
      cgtgcatgc tgacagctg aaaatgcac acgagagtg 
      atgttaatg ttataagtt ggaataaaa acgaaccct 
      ttattgtca gtggtggag acgatggat ttttacaca 
      tttgggatt tgcggaggt ttcaacaaa aaacaccag 
      tcgccacat ttaagcatc acacagagc cagtgacta 
      ccgttgaat ggcatccaa ctgattcag ctgtgttta 
      tctcagggg gaagtgata atcaagtgg cattatggg 
      atttgtctg tggaaaagg atgatgaga gtggtagtg 
      aagaagttg atggaattc cgccccaac ttttgttca 
      tccatcaag gacaaaata atataaagg aattgcact 
      ggcatccac aactgccag gagtgataa tcagtacgg 
      cggagagtg gttttaata tattcagaa ctatcagcg 
      tttaaaata ttttaataa attgaaaca aatgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY