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Gene information
General information
TC002935 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6046339..6047398
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002935
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09758

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber mostlynot present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber potentiallydegenerated - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002935 RA
      MEDPKIEDL DLYEILEIE TTSTVADIK KAYRKKALQ 
      CHPDKNPDN PNAAKEFHQ LSRILEVLI DATARKAYD 
      AVLKGRKEA AIRHKELDS KRRKLKEDL EARERRAAA 
      NYKTKSADE KLKEEIERL RKEGSKQVE EELERIRQE 
      VLEEQKAQL DANKCSGAN YRIKIKWNV AKDDTTNGG 
      YSHENLHRF LSKYGNVTA LVLSQKRMG TALVEFEAR 
      NAAEMAVEM EVGLPSNPL KLEWVNPPP ARKGAASSL 
      VKPSDYESV VLTKMRQAE ERRRLIEQL KAEDGDD
    CDS sequence
    • >TC002935 RA
      atggaagac ccgaaaatc gaggattta gatttgtat 
      gaaattctg gaaattgaa acaaccagc acagtcgca 
      gatatcaaa aaagcgtac aggaaaaag gcccttcag 
      tgccatcct gacaaaaac ccggataat ccaaatgca 
      gcgaaagaa tttcaccag ctgtcccga attcttgaa 
      gtattgata gacgcaact gccagaaag gcatatgat 
      gctgtccta aaaggccgt aaagaagct gcgataaga 
      cacaaggaa cttgatagt aaaagaaga aaactgaaa 
      gaggatttg gaagctcgt gaacgtcgt gcagccgcc 
      aattataaa acgaaatct gcagatgag aaactgaaa 
      gaagaaatt gaaaggcta cgcaaggaa ggttccaaa 
      caagtggaa gaagagctt gagagaatc agacaggaa 
      gttttggaa gaacaaaaa gctcaactt gacgctaac 
      aagtgtagt ggtgcaaat tacagaatc aaaatcaag 
      tggaacgtt gctaaagac gatacaaca aatgggggg 
      tacagtcat gagaacctc cacagattt ttgtcaaag 
      tatggtaat gtgaccgcc ttagtattg tctcagaaa 
      agaatgggt actgcactt gttgaattt gaggcaaga 
      aatgcagca gaaatggcc gtggagatg gaagtaggg 
      ctcccgtcc aatccttta aaactcgaa tgggtgaac 
      ccacccccc gcacgtaag ggtgccgcc agttccctt 
      gtgaagccc tcagactat gaaagtgtg gtactgaca 
      aaaatgaga caagcagag gagaggcgg aggctgatt 
      gaacagttg aaagcagag gatggagat gattaa
    mRNA sequence
    • >TC002935 RA
      caaacaaaa atggaagac ccgaaaatc gaggattta 
      gatttgtat gaaattctg gaaattgaa acaaccagc 
      acagtcgca gatatcaaa aaagcgtac aggaaaaag 
      gcccttcag tgccatcct gacaaaaac ccggataat 
      ccaaatgca gcgaaagaa tttcaccag ctgtcccga 
      attcttgaa gtattgata gacgcaact gccagaaag 
      gcatatgat gctgtccta aaaggccgt aaagaagct 
      gcgataaga cacaaggaa cttgatagt aaaagaaga 
      aaactgaaa gaggatttg gaagctcgt gaacgtcgt 
      gcagccgcc aattataaa acgaaatct gcagatgag 
      aaactgaaa gaagaaatt gaaaggcta cgcaaggaa 
      ggttccaaa caagtggaa gaagagctt gagagaatc 
      agacaggaa gttttggaa gaacaaaaa gctcaactt 
      gacgctaac aagtgtagt ggtgcaaat tacagaatc 
      aaaatcaag tggaacgtt gctaaagac gatacaaca 
      aatgggggg tacagtcat gagaacctc cacagattt 
      ttgtcaaag tatggtaat gtgaccgcc ttagtattg 
      tctcagaaa agaatgggt actgcactt gttgaattt 
      gaggcaaga aatgcagca gaaatggcc gtggagatg 
      gaagtaggg ctcccgtcc aatccttta aaactcgaa 
      tgggtgaac ccacccccc gcacgtaag ggtgccgcc 
      agttccctt gtgaagccc tcagactat gaaagtgtg 
      gtactgaca aaaatgaga caagcagag gagaggcgg 
      aggctgatt gaacagttg aaagcagag gatggagat 
      gattaaatt ataagagaa ataaacgtt ttattattt 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY