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Gene information
General information
TC002947 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5893328..5899452
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002947
    iBeetle screen iB_09755

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002947 RA
      MGYDDVIPL IGDFGGYQK RIYFLLCLP AILCAFHKL 
      GNVFLIAEP AYRCKLPSE FSNATYQDL SPEEMNKSY 
      PWDSLKKNF SSCEMFVGN ATKRCDEFV YDESVYGYT 
      AVIEWELTC EKAYMIATG NSLFMVGVM LGSIIFGQL 
      SDKYGRKKI FFASLIIQV FFGLIAAFT PEFWSFTLS 
      RAIVGATTS GVFLVAYVI ALEMVSPKK RLLAGTCCQ 
      MFFSTGYML TALFAIYIH EWRDLQLAL TLPGLVFFC 
      YWWLIPESS RWLINQNRV EEAKVLIKI AAKKNKIKI 
      TDEQLDSLL VSEVRPENH NAHKANVLD IFRHSNLRK 
      RSLIILFDW FANNITYYG LSWNTNNLA GNPYINFVI 
      SGAVEIPAY TFLLFTLNR WGRKFVMCG CMIFAGVAL 
      LLTMAIPDE HEWLVVTCA MLGKLAITA SYGAIYIFS 
      TEQFPTVIR NAGLGAGST CARIGSITS PYINVLSKF 
      WTPLPLIIF GSLALIGGV MSLVLPETL NKKLPETME 
      EGEAFGKKV KKERTKEEV PLNGNDLEK QAKVETNGG 
      IQPN
    CDS sequence
    • >TC002947 RA
      atgggctat gacgatgtt attccccta atcggcgat 
      ttcggggga taccagaag cggatttat tttttatta 
      tgccttcca gccattctc tgtgccttt cataagctg 
      ggaaatgtg ttcctcatc gcagaacct gcttacaga 
      tgtaaactt ccgagcgag ttttccaac gccacttac 
      caggacctg agtcccgag gaaatgaac aagtcttat 
      ccttgggac tcgctgaaa aagaacttt tcatcatgc 
      gaaatgttc gtcgggaat gcgacaaaa agatgcgat 
      gaattcgtt tacgacgaa agtgtctac ggatacacc 
      gcagttatc gaatgggaa ctcacgtgc gagaaggcg 
      tacatgata gccaccggc aactcactt ttcatggtc 
      ggagtcatg ctaggttcc ataattttc ggccaatta 
      tccgacaaa tacggccgc aagaaaatc tttttcgct 
      tccttgatt atccaggtc tttttcggc ttgatcgcg 
      gctttcacc cccgaattt tggtcattc acgctgtcc 
      cgggccata gtgggggcc acgacttcg ggggttttc 
      ctcgtagct tacgtaatc gctttggag atggtgagc 
      ccgaagaag cggctcttg gctgggacc tgctgccag 
      atgttcttc tcaactggc tacatgctg acggccctt 
      tttgccatc tacatccac gagtggagg gacctccaa 
      ctggctctg actctcccc ggacttgtg tttttctgt 
      tactggtgg ttgattccg gagtcctcc cgctggcta 
      atcaaccaa aatcgcgtg gaggaagcc aaagtcctt 
      atcaaaatc gcggccaaa aagaacaaa atcaaaata 
      accgacgaa caactggac agcttgctg gtttcggaa 
      gtcagacct gaaaaccac aacgcgcat aaagccaac 
      gtgttggac attttcaga cattccaat ttacgaaaa 
      cgatctctg attatatta ttcgattgg tttgccaac 
      aacatcact tactacggc ctttcctgg aacacgaat 
      aacttggcc gggaacccg tacattaac ttcgtgatt 
      tcgggggct gttgagata cccgcttac acatttttg 
      ctgttcact ctgaacaga tgggggcgc aagttcgta 
      atgtgcggc tgcatgatc tttgccggc gtggctttg 
      ttactgacg atggcgatt cctgacgaa cacgaatgg 
      ttggttgta acttgcgcc atgttggga aaactggcg 
      ataacggca agttacggc gctatttat attttttcg 
      accgagcaa ttccccaca gtgataaga aacgccgga 
      ttgggggct ggttcgacc tgcgctcga attgggtca 
      attacatcg ccctacatc aacgttttg agcaaattc 
      tggacccct ctcccgttg ataattttc ggttctttg 
      gcgctcatt ggcggagta atgtcgttg gtgttgccg 
      gaaactttg aacaaaaaa ttaccagag accatggaa 
      gagggtgaa gcctttgga aagaaagtg aaaaaggaa 
      cgaacgaag gaagaagtg ccgctcaac ggaaacgac 
      cttgagaag caggcgaaa gttgagacc aacggcggc 
      atacaaccg aactga
    mRNA sequence
    • >TC002947 RA
      tctcgtcga gaccgcttt gttagtgct gattacttt 
      tattacgct cacaggatt acgtttttt gtttaattt 
      aacgctgta ttccgtgaa aaagtgcca aattcgtta 
      cccttctaa aagagggtt gttagtaaa aaattggaa 
      acgtgaaaa ctggtcttt tttaaacaa gttacgaaa 
      atcgaatgt ttcgagcgc ttattcaac gcatggcgt 
      gactctgca ttggaccag atcaatttt tcggatttt 
      ccatagagg tcagcataa gtattgatt tggaagatt 
      gcacactgg taaccccga tttagcccg aaacgtgta 
      gatgttaga tttccgaag cagagtgtt acacttcta 
      tgtgagctc ttctagtct ttgaccatc aaatcggtg 
      acggtacaa tgggctatg acgatgtta ttcccctaa 
      tcggcgatt tcgggggat accagaagc ggatttatt 
      ttttattat gccttccag ccattctct gtgcctttc 
      ataagctgg gaaatgtgt tcctcatcg cagaacctg 
      cttacagat gtaaacttc cgagcgagt tttccaacg 
      ccacttacc aggacctga gtcccgagg aaatgaaca 
      agtcttatc cttgggact cgctgaaaa agaactttt 
      catcatgcg aaatgttcg tcgggaatg cgacaaaaa 
      gatgcgatg aattcgttt acgacgaaa gtgtctacg 
      gatacaccg cagttatcg aatgggaac tcacgtgcg 
      agaaggcgt acatgatag ccaccggca actcacttt 
      tcatggtcg gagtcatgc taggttcca taattttcg 
      gccaattat ccgacaaat acggccgca agaaaatct 
      ttttcgctt ccttgatta tccaggtct ttttcggct 
      tgatcgcgg ctttcaccc ccgaatttt ggtcattca 
      cgctgtccc gggccatag tgggggcca cgacttcgg 
      gggttttcc tcgtagctt acgtaatcg ctttggaga 
      tggtgagcc cgaagaagc ggctcttgg ctgggacct 
      gctgccaga tgttcttct caactggct acatgctga 
      cggcccttt ttgccatct acatccacg agtggaggg 
      acctccaac tggctctga ctctccccg gacttgtgt 
      ttttctgtt actggtggt tgattccgg agtcctccc 
      gctggctaa tcaaccaaa atcgcgtgg aggaagcca 
      aagtcctta tcaaaatcg cggccaaaa agaacaaaa 
      tcaaaataa ccgacgaac aactggaca gcttgctgg 
      tttcggaag tcagacctg aaaaccaca acgcgcata 
      aagccaacg tgttggaca ttttcagac attccaatt 
      tacgaaaac gatctctga ttatattat tcgattggt 
      ttgccaaca acatcactt actacggcc tttcctgga 
      acacgaata acttggccg ggaacccgt acattaact 
      tcgtgattt cgggggctg ttgagatac ccgcttaca 
      catttttgc tgttcactc tgaacagat gggggcgca 
      agttcgtaa tgtgcggct gcatgatct ttgccggcg 
      tggctttgt tactgacga tggcgattc ctgacgaac 
      acgaatggt tggttgtaa cttgcgcca tgttgggaa 
      aactggcga taacggcaa gttacggcg ctatttata 
      ttttttcga ccgagcaat tccccacag tgataagaa 
      acgccggat tgggggctg gttcgacct gcgctcgaa 
      ttgggtcaa ttacatcgc cctacatca acgttttga 
      gcaaattct ggacccctc tcccgttga taattttcg 
      gttctttgg cgctcattg gcggagtaa tgtcgttgg 
      tgttgccgg aaactttga acaaaaaat taccagaga 
      ccatggaag agggtgaag cctttggaa agaaagtga 
      aaaaggaac gaacgaagg aagaagtgc cgctcaacg 
      gaaacgacc ttgagaagc aggcgaaag ttgagacca 
      acggcggca tacaaccga actgaaaca ttactagga 
      cagtattag aattatttt ttatataag tactattgt 
      ttcgtttta ttattattt agaagggtg attcagttt 
      tatttacat cacaaaatt ttacgattg tttgtaaaa 
      actagtcat tgtgttctt tcctttgaa aaattgacg 
      ttatgaaag tggtgccaa agagcggtc gtttcattt 
      ttattctcg aacaatttt aagacgcag tagagattt 
      gcataaaga atggcattg aaatcgaac tgcgattaa 
      aatacttgt tgcacaaga ttgtcgggt ttttacaac 
      taaaaagag ttgtgattt aagcgcttc ttaagaaca 
      cgagtctgt tatgtttta tgggaaatg tttgtagaa 
      tctgtttgc atttaaatt acgataatt gaaaaatta 
      ccattgtgg tcgtccagt tgcttattg ggacgataa 
      taagattat aatgggcaa attgtgtct gagactgac 
      tagggaatt tcctttaca tgtttcact aattagatg 
      caggtttga tttactgtc attatttta atcgtttac 
      gttgtctca ataatttat tcatttatt gcagtactt 
      atatcactt caaaacaag ttaatttac ttatgttac 
      ttttaattt gttcgttta ttacgacaa atgggccca 
      ttttaagaa agtgtactg ttgcctctt actcttaaa 
      atgtttgaa gctttaaaa gcgctcgaa tttcgtact 
      tgttcctcc tatgataaa ttttgtgaa acagcgggt 
      tttaaacct acttctgta tgtttttaa ttgttttta 
      ctcttactc tcaatgttt taaaaaatt cgattgttg 
      ttttttgta attttaaaa ctacccatg tgaatgcat 
      ttataaatt ttatataaa atattaaaa aaataaatg 
      tcgcaaagt gaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY