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Gene information
General information
TC003485 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5868024..5872079
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003485
    iBeetle screen iB_09754

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003485 RA
      MPIELQTED RLEYTFFPN ASGLLQFRV RAPNDAHIA 
      LSPSASEAT PMYEVFIGG WGNSKSVIR KNRTKPDVA 
      EASTPGFLN PDEFRGFWI RWESGLISV GHEGNAAPF 
      LEWRDFEQV PIEYVGVCT GWGATGAWI IEEARGGAP 
      AMGSRGNFS NVCWVAARN GEVPPRAFA GGEDNGEPV 
      YVARANFNG GLIPGKLVA SHGTAYVPW GGQENAVPE 
      YEVLCDFPG NWIACSGGN VPPNAVTAG QSEEGEPLY 
      VGRVVHDGS LTVGKVQPS HGVVYIPYG GTELGFQDY 
      EILVQ
    CDS sequence
    • >TC003485 RA
      atgcctatc gaattgcaa accgaggac cgcctcgaa 
      tacaccttt ttcccgaac gcctccggg ctcctgcaa 
      ttccgcgtg cgcgccccc aacgatgcc catatcgcc 
      ctcagcccc tccgcgtcc gaggccacc cccatgtac 
      gaggtcttc atcggcggc tggggcaac tccaaatca 
      gtgatccgc aagaaccgc accaaaccc gacgtggcc 
      gaggccagc acgcccggc ttcctcaac cccgacgag 
      ttccgcggc ttctggatc cgctgggag agcggcctc 
      atctccgtg ggccacgag ggcaacgcc gcccccttc 
      ctcgagtgg cgcgacttc gagcaggtg cccatcgag 
      tacgtgggc gtgtgcacg ggctggggc gccacgggc 
      gcctggatc attgaggag gcgcgcggc ggcgccccc 
      gcgatgggg tcccgcggc aacttctcc aacgtgtgc 
      tgggtggcg gcgcgcaac ggcgaggtg ccccccagg 
      gccttcgcc ggcggcgag gacaacggc gagcccgtt 
      tacgtcgcc agagccaac ttcaacggg gggctgatc 
      cccgggaag ctggtcgcg agccacggc accgcctac 
      gtgccctgg ggcgggcag gagaacgcg gtccccgag 
      tacgaggtg ctgtgcgac ttccccggg aactggatc 
      gcctgcagc ggcggcaac gtgcccccg aatgcggtc 
      actgcgggg cagagcgag gagggagag cccctgtac 
      gtgggcagg gtggtccac gatgggtcg ctcacggtg 
      gggaaggtg cagcccagc catggggtg gtttatatt 
      ccctatggg gggaccgag ttagggttc caggattat 
      gaaattttg gtacaataa
    mRNA sequence
    • >TC003485 RA
      gtcagtttg aattgttga cctgtgtgg tgttcaggt 
      ggaatttca taaataaat tgcaacatg cctatcgaa 
      ttgcaaacc gaggaccgc ctcgaatac acctttttc 
      ccgaacgcc tccgggctc ctgcaattc cgcgtgcgc 
      gcccccaac gatgcccat atcgccctc agcccctcc 
      gcgtccgag gccaccccc atgtacgag gtcttcatc 
      ggcggctgg ggcaactcc aaatcagtg atccgcaag 
      aaccgcacc aaacccgac gtggccgag gccagcacg 
      cccggcttc ctcaacccc gacgagttc cgcggcttc 
      tggatccgc tgggagagc ggcctcatc tccgtgggc 
      cacgagggc aacgccgcc cccttcctc gagtggcgc 
      gacttcgag caggtgccc atcgagtac gtgggcgtg 
      tgcacgggc tggggcgcc acgggcgcc tggatcatt 
      gaggaggcg cgcggcggc gcccccgcg atggggtcc 
      cgcggcaac ttctccaac gtgtgctgg gtggcggcg 
      cgcaacggc gaggtgccc cccagggcc ttcgccggc 
      ggcgaggac aacggcgag cccgtttac gtcgccaga 
      gccaacttc aacgggggg ctgatcccc gggaagctg 
      gtcgcgagc cacggcacc gcctacgtg ccctggggc 
      gggcaggag aacgcggtc cccgagtac gaggtgctg 
      tgcgacttc cccgggaac tggatcgcc tgcagcggc 
      ggcaacgtg cccccgaat gcggtcact gcggggcag 
      agcgaggag ggagagccc ctgtacgtg ggcagggtg 
      gtccacgat gggtcgctc acggtgggg aaggtgcag 
      cccagccat ggggtggtt tatattccc tatgggggg 
      accgagtta gggttccag gattatgaa attttggta 
      caataaagg actgatgga tggattttg ttaatcgct 
      ttaatttgt tgctgccta ggaattcga tccagcata 
      aatcaaatg ttattaagg ttagataac gctagactg 
      caccgttgt ggcttctag gtgccttat gtaatgtgt 
      accgatatg aagtgtatt atttttgta taatggaaa 
      cggtctcga aatttttta tttcgtaat agtggctga 
      tttggttgg tgccactct aatatagtt ttttaagtc 
      tataatgta ataaaaaga ataaaattt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY