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Gene information
General information
TC032393
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030732
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5793670..5797372
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032393
    iBeetle screen iB_09753

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032393 RA
      MKNRKKILL PNKPIIKRS ESHIDRSHS DKPLKKQIF 
      TSVSLHQNL EFFRQQSNL FPKPLPNYG DADTYFRQS 
      LRNYNYFDI SFFGKKRNS GVKSARSVE SLKKKKSSS 
      SVKPKTPST DPRSPILKL KLPESKKET EEFDADIFY 
      SPDSQKSFK ESARTLTHN KSDSSLIEE DEKSGEITP 
      VNMAEDAED FAEEYEAQA EAEPAPIVG DETETEGEE 
      EEGEDEMKQ VEQMIKQAS AELAEVTEE NQQLIETFT 
      EEEEEGELD EGEGAPMRA AGDGSTFKF DPAIDAAVL 
      RELEEAEKQ AKATAKIIY ELKTRVTEL LKKEKNTEA 
      EARELEEKN KELKAQMLL FEEKTKRIQ FLIAQTNLF 
      DKMIPPQTP LQTPHNEDV LPKVIVCGM TEHNIPKLL 
      LCDDKKKSC SPSCDKNVN IPMSVVPKF AKRLSDSYT 
      MQEKLAAEN ADLEGMRYK LQSDLLNKD QTIEYLQRK 
      LCSIQCEMR VLCKENSEL NEKVQQICG HKQDVQKPK 
      KMPCGKGRG PCPADIEHR LNEYSETTK ILEKQLCDM 
      ENDVKTMQS ELTAVQTER EHLEQHKKI IICPPPMCH 
      PCAPPPCPP CSPCIVPCS DLQLRELRE QYCRLQDDF 
      KSKVTEVGG LRADNEKLK ATAKEAEEA QKKLEDRVR 
      ELERTLKSF KTDNNKFVG SKEQLIEQE QQLAVAKQR 
      FREAQDELE ELRSFIQDQ QGQLDDYRN KYLEAQQEV 
      EEQRRHIDM LEMDANRVN EQVNLEIQR VKSQFQEKL 
      QELLPLPDL LKTTQLKLQ EAQQMHLLA ERNNEALQR 
      DLQLYKDKI AEITGEMDK ARSDNKLGE NEKLSLAQR 
      IEEMEAKIN ELEEENFSL REDMKRTEE TLEETEREK 
      EAKMHEIAQ LAAQLETVR EESARQVAR NKERCETVR 
      RSMQNQISD LERQLAQSR AQARAAQKD RDEIRQKMQ 
      AQINNLHEN FEDAQMRIR NLQGHVNFL KESYTPNVP 
      GVGDTESVS DHQDPCNCG RDF
    CDS sequence
    • >TC032393 RA
      atgaaaaat agaaagaaa attcttctg ccaaataag 
      ccgattatt aaacgctcg gagtcgcac atcgacaga 
      agtcactcg gacaagcct ctgaaaaag caaatattc 
      accagtgtt tcgttgcat cagaatttg gagtttttt 
      cggcaacaa agcaactta tttcccaaa cctctaccg 
      aattacgga gacgccgac acttatttt agacaaagt 
      ttacgaaac tataactat tttgatatc tcatttttc 
      ggtaaaaaa cggaacagt ggtgttaaa agtgccagg 
      tctgtcgaa agtttaaaa aagaagaaa tcttcgtcg 
      tcagtaaag ccaaaaaca ccctcgaca gatccgagg 
      agtccgatt ttaaaatta aaattacct gaaagcaaa 
      aaagaaact gaagaattt gatgcagac atcttctat 
      tcaccagac agtcagaaa agttttaag gaatcagct 
      cgtactcta acacataac aaatctgat agtagctta 
      atagaagag gacgagaaa tctggtgaa ataactcca 
      gttaacatg gctgaagat gctgaagat tttgctgag 
      gaatatgaa gcacaggct gaagctgaa cccgcacca 
      atcgtggga gatgaaaca gaaaccgaa ggcgaagaa 
      gaggaaggg gaagatgaa atgaaacaa gttgaacag 
      atgataaag caagcatca gcagaacta gctgaagta 
      accgaggaa aatcaacaa cttatagaa acctttact 
      gaagaggaa gaagaaggg gagctggac gaaggagaa 
      ggagcccca atgagagca gctggtgac ggctcaacc 
      ttcaagttt gacccagca attgatgct gcggttctc 
      agagaacta gaagaagca gaaaaacaa gccaaagct 
      acggccaaa ataatctac gaactgaaa actcgcgtt 
      actgaattg ctaaagaaa gagaaaaac actgaagct 
      gaggctagg gaactggag gagaaaaat aaagaactc 
      aaagctcaa atgcttctg tttgaggag aaaaccaaa 
      cgaattcaa ttcctcatc gcccaaacc aacctattc 
      gataaaatg ataccaccg caaacccct ctccagacg 
      ccccataac gaagatgtg ctgccgaaa gtaatcgtc 
      tgcggaatg accgagcat aatattccg aaattactg 
      ttgtgtgat gataaaaag aaatcctgt tctccgtct 
      tgtgacaaa aatgtaaac attccaatg tctgttgtg 
      cccaaattt gccaaaagg ctcagtgat agttacacg 
      atgcaggaa aaattagcc gctgaaaat gccgattta 
      gaaggcatg aggtacaaa ttacagtcc gacctactc 
      aacaaagac caaacgatt gaatacctc caaagaaaa 
      ctttgcagc attcagtgt gaaatgcga gtcctctgc 
      aaggaaaac tcagaactg aacgaaaaa gtgcaacag 
      atttgcggc cacaaacaa gacgtccag aaaccgaaa 
      aaaatgccg tgtgggaaa ggaagaggg ccatgtcct 
      gccgatatc gagcaccga ctcaatgaa tattccgaa 
      accaccaaa attctggaa aaacaactc tgcgatatg 
      gaaaacgac gtaaaaacg atgcaaagt gaactaact 
      gctgtacaa accgaacgg gaacattta gaacaacac 
      aagaaaatt atcatttgc cctcccccc atgtgccac 
      ccttgtgcc cctcctccg tgtcctccc tgcagcccg 
      tgcattgtc ccttgttcc gatttgcaa ttgagggaa 
      ttgagggaa caatactgc agactccag gatgatttc 
      aaaagcaag gtgactgaa gttggggga cttagggct 
      gataacgaa aaattgaaa gcgacggct aaagaagca 
      gaagaggcg caaaagaaa cttgaagac agagtgcga 
      gaattggag aggacgtta aaaagtttc aagactgat 
      aataacaaa tttgtggga tcgaaggaa cagttaata 
      gaacaggaa cagcagctc gcagttgcg aaacaaaga 
      ttcagagaa gcgcaagat gagttggag gaattgaga 
      tcgtttatt caagaccag cagggacaa cttgatgac 
      tacagaaat aaatatttg gaagcccaa caagaagtc 
      gaggaacaa cgaagacac attgacatg ttggaaatg 
      gacgcgaat cgagtgaac gagcaagtg aatttagag 
      atccagcgc gtgaaaagc cagtttcaa gagaaattg 
      caagagctt ttgccgctt cccgattta ctgaaaacc 
      acccaactg aagctgcaa gaagcgcaa cagatgcac 
      cttctcgct gagcgcaac aacgaggct cttcagcga 
      gacttgcag ctgtataag gacaaaatt gccgagatt 
      accggcgag atggataag gctcgcagt gacaacaaa 
      ttaggcgaa aacgagaaa ctgtcgttg gcgcaaagg 
      attgaggaa atggaggcg aaaataaac gaactggag 
      gaggagaat ttcagtctg agggaagac atgaagcga 
      actgaagaa accttggaa gaaaccgag cgagaaaag 
      gaagcaaaa atgcacgaa attgcccag ctagccgcc 
      cagttggag acggtccgt gaggaatct gcccggcaa 
      gttgccagg aataaggag aggtgtgaa acggttagg 
      aggtcgatg cagaaccaa atatccgat ttggagaga 
      cagctggct caaagcagg gcccaggca agggctgca 
      cagaaggac agggacgaa attcggcaa aaaatgcaa 
      gcgcagatt aataatctt cacgaaaac ttcgaagat 
      gcgcaaatg cggattaga aacctacaa gggcacgtt 
      aatttcctg aaagagtcg tatactccg aatgttccc 
      ggagttggg gataccgaa tcggtgtcc gatcatcaa 
      gatccttgc aactgcgga cgagatttt tga
    mRNA sequence
    • >TC032393 RA
      agttcttaa ttttttgtt ccattttgc gtcaatatt 
      ctatttata ctgcttttt gtaccacat tattgcaat 
      ttctacgag tttcctgct atcccaccg tgtgttaca 
      gacacattt ctttcggaa cgtgacttt ttcctgaat 
      aatgaaaaa tagaaagaa aattcttct gccaaataa 
      gccgattat taaacgctc ggagtcgca catcgacag 
      aagtcactc ggacaagcc tctgaaaaa gcaaatatt 
      caccagtgt ttcgttgca tcagaattt ggagttttt 
      tcggcaaca aagcaactt atttcccaa acctctacc 
      gaattacgg agacgccga cacttattt tagacaaag 
      tttacgaaa ctataacta ttttgatat ctcattttt 
      cggtaaaaa acggaacag tggtgttaa aagtgccag 
      gtctgtcga aagtttaaa aaagaagaa atcttcgtc 
      gtcagtaaa gccaaaaac accctcgac agatccgag 
      gagtccgat tttaaaatt aaaattacc tgaaagcaa 
      aaaagaaac tgaagaatt tgatgcaga catcttcta 
      ttcaccaga cagtcagaa aagttttaa ggaatcagc 
      tcgtactct aacacataa caaatctga tagtagctt 
      aatagaaga ggacgagaa atctggtga aataactcc 
      agttaacat ggctgaaga tgctgaaga ttttgctga 
      ggaatatga agcacaggc tgaagctga acccgcacc 
      aatcgtggg agatgaaac agaaaccga aggcgaaga 
      agaggaagg ggaagatga aatgaaaca agttgaaca 
      gatgataaa gcaagcatc agcagaact agctgaagt 
      aaccgagga aaatcaaca acttataga aacctttac 
      tgaagagga agaagaagg ggagctgga cgaaggaga 
      aggagcccc aatgagagc agctggtga cggctcaac 
      cttcaagtt tgacccagc aattgatgc tgcggttct 
      cagagaact agaagaagc agaaaaaca agccaaagc 
      tacggccaa aataatcta cgaactgaa aactcgcgt 
      tactgaatt gctaaagaa agagaaaaa cactgaagc 
      tgaggctag ggaactgga ggagaaaaa taaagaact 
      caaagctca aatgcttct gtttgagga gaaaaccaa 
      acgaattca attcctcat cgcccaaac caacctatt 
      cgataaaat gataccacc gcaaacccc tctccagac 
      gccccataa cgaagatgt gctgccgaa agtaatcgt 
      ctgcggaat gaccgagca taatattcc gaaattact 
      gttgtgtga tgataaaaa gaaatcctg ttctccgtc 
      ttgtgacaa aaatgtaaa cattccaat gtctgttgt 
      gcccaaatt tgccaaaag gctcagtga tagttacac 
      gatgcagga aaaattagc cgctgaaaa tgccgattt 
      agaaggcat gaggtacaa attacagtc cgacctact 
      caacaaaga ccaaacgat tgaatacct ccaaagaaa 
      actttgcag cattcagtg tgaaatgcg agtcctctg 
      caaggaaaa ctcagaact gaacgaaaa agtgcaaca 
      gatttgcgg ccacaaaca agacgtcca gaaaccgaa 
      aaaaatgcc gtgtgggaa aggaagagg gccatgtcc 
      tgccgatat cgagcaccg actcaatga atattccga 
      aaccaccaa aattctgga aaaacaact ctgcgatat 
      ggaaaacga cgtaaaaac gatgcaaag tgaactaac 
      tgctgtaca aaccgaacg ggaacattt agaacaaca 
      caagaaaat tatcatttg ccctccccc catgtgcca 
      cccttgtgc ccctcctcc gtgtcctcc ctgcagccc 
      gtgcattgt cccttgttc cgatttgca attgaggga 
      attgaggga acaatactg cagactcca ggatgattt 
      caaaagcaa ggtgactga agttggggg acttagggc 
      tgataacga aaaattgaa agcgacggc taaagaagc 
      agaagaggc gcaaaagaa acttgaaga cagagtgcg 
      agaattgga gaggacgtt aaaaagttt caagactga 
      taataacaa atttgtggg atcgaagga acagttaat 
      agaacagga acagcagct cgcagttgc gaaacaaag 
      attcagaga agcgcaaga tgagttgga ggaattgag 
      atcgtttat tcaagacca gcagggaca acttgatga 
      ctacagaaa taaatattt ggaagccca acaagaagt 
      cgaggaaca acgaagaca cattgacat gttggaaat 
      ggacgcgaa tcgagtgaa cgagcaagt gaatttaga 
      gatccagcg cgtgaaaag ccagtttca agagaaatt 
      gcaagagct tttgccgct tcccgattt actgaaaac 
      cacccaact gaagctgca agaagcgca acagatgca 
      ccttctcgc tgagcgcaa caacgaggc tcttcagcg 
      agacttgca gctgtataa ggacaaaat tgccgagat 
      taccggcga gatggataa ggctcgcag tgacaacaa 
      attaggcga aaacgagaa actgtcgtt ggcgcaaag 
      gattgagga aatggaggc gaaaataaa cgaactgga 
      ggaggagaa tttcagtct gagggaaga catgaagcg 
      aactgaaga aaccttgga agaaaccga gcgagaaaa 
      ggaagcaaa aatgcacga aattgccca gctagccgc 
      ccagttgga gacggtccg tgaggaatc tgcccggca 
      agttgccag gaataagga gaggtgtga aacggttag 
      gaggtcgat gcagaacca aatatccga tttggagag 
      acagctggc tcaaagcag ggcccaggc aagggctgc 
      acagaagga cagggacga aattcggca aaaaatgca 
      agcgcagat taataatct tcacgaaaa cttcgaaga 
      tgcgcaaat gcggattag aaacctaca agggcacgt 
      taatttcct gaaagagtc gtatactcc gaatgttcc 
      cggagttgg ggataccga atcggtgtc cgatcatca 
      agatccttg caactgcgg acgagattt ttgaaaatt 
      ttattctgt aatgggact gtaaacaaa tcacctgaa 
      atatatttc gataaacca t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY