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Gene information
General information
TC002978 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5427020..5431777
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002978
    iBeetle screen iB_09749

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002978 RA
      MPASTLEPV NAALRSALT KTDDSSLDT QLAGSTKKA 
      LLSNIDFEP AGSYQSTVL DKLKSKYIV LKSSETPQP 
      SKPEGDMGA DETLKLASY ELYPFEAVQ LGWSKCDWS 
      VGAGMINMG NTCYLNSTL QALFHVPAL VNWLISDKE 
      HTAECQDSG GLCIICAMR KTLQDSQQR NTNTIRPLL 
      IYNKLRLVC RNLIPGRQE DAHEFLRYL VEAMEKSYL 
      SRFKNHSEF DSKVKETTP LNQILGGYL RSAVRCLKC 
      GHVSTTFQH FQDLLLDIR KAQTLDEAL EGYFSREKL 
      DDDSYHCQS CQKKVPATK QFSLERAPM VLCIQLKRF 
      SVSNNKITK HIHFRQRLD LTRYARHRP SVPLIYRLV 
      ALVTHMGPT VNCGHYTAV AQAPSGNFY QFDDSMVRV 
      ISHQAVFNT NAYIMLYEL ESSPFTKSN NGTNNKIKP 
      PLSPEANTS NNTSKASLN GIGFTSDKV YGPELPPDR 
      VEKKINGVN PTPVTNGNG NHSESSGTS DSEPENPQK 
      SPQKENKPV ATPPLPPKK SMPSINNEE TAKSPLMQK 
      NRTLSPRNT NSTTPISST NAEKKGSFQ PVTTKLVPY 
      ETDDSSCSD ESNQSPQDV EYRVSTKAA VGDWKVTST 
      EPTTQSKSW GEKKQDGTV SELFKMSHS GYSAPVSSW 
      NGTRSQLDK EISNERRED RKRALSDNS DQGKVKHQK 
      LNNSYKSNP GYNPVQEFH NTKNWSQNN GYKPFYSGN 
      RHRRNFHHK SFHKNYRFR Q
    CDS sequence
    • >TC002978 RA
      atgccggcg tccaccctc gagcccgtg aacgcggct 
      ttgcgctcc gccctcacc aagaccgac gacagttct 
      ctggacacg cagttggcg ggatccacc aagaaggcc 
      ctcctgtcc aacatcgac ttcgagccg gccgggagc 
      taccaaagc accgtcttg gacaaactc aagtcgaag 
      tacatcgtg ttgaaaagc tcggagact ccgcagccc 
      agcaagcct gagggagac atgggcgcg gatgaaacg 
      ctcaaattg gctagttac gagctgtat ccttttgaa 
      gctgtgcag ttaggttgg agtaaatgt gactggtca 
      gtgggggcc ggcatgatt aacatggga aatacctgt 
      tatctcaac tctacgctt caagcactt ttccacgtt 
      cccgctctt gtcaattgg ctcatttct gataaggaa 
      cacacagct gaatgccag gactcaggt gggctgtgc 
      ataatatgt gcaatgcgg aagactctc caagactcc 
      caacaacgt aacacaaac acaatccgt cctctcctc 
      atctacaac aagctgcgt ttggtgtgt cgaaacctc 
      atcccgggt cggcaagaa gatgcgcac gaatttctt 
      agatatcta gtggaagca atggaaaaa tcctattta 
      agtcgattt aaaaaccat tccgaattt gattcaaaa 
      gtgaaagag accacacct ctcaatcaa atactaggc 
      ggatattta agatcggca gtgagatgt ttgaagtgt 
      ggtcacgtc agcaccacc ttccaacac tttcaagat 
      ttactatta gacattagg aaagctcaa acgctcgac 
      gaggcgttg gaaggttac ttctctagg gaaaaattg 
      gacgatgac tcgtatcat tgtcagtct tgtcagaaa 
      aaagtacct gcaacaaaa cagttctcc cttgaaaga 
      gcccctatg gttctttgt atacaactc aaacggttt 
      tctgttagc aataataaa attactaaa catatacat 
      tttcgacaa agactggat ctaacaagg tatgccaga 
      catcgtccc agtgttcca ctgatctat agacttgtt 
      gccttagtc acacatatg ggacctact gttaattgt 
      ggccactac actgcagtt gcccaagcg ccgtctggt 
      aatttttac cagtttgat gatagtatg gtgcgggtg 
      atatctcac caagctgtg ttcaataca aacgcatac 
      ataatgctg tacgaatta gagtcatct ccatttacc 
      aaatcaaat aacgggaca aacaacaag ataaaacct 
      ccgctttct cccgaagca aatactagc aacaacaca 
      tcaaaagct tcactgaac ggtatcggt tttacgagc 
      gacaaagtc tacggcccc gaactgccc cccgaccga 
      gtagaaaag aaaatcaac ggtgttaac cccacaccg 
      gtcactaac ggtaacgga aatcactca gagagttcc 
      ggaacgagc gacagcgaa ccggaaaat ccgcaaaaa 
      agtccacaa aaagaaaac aaaccggtg gcgacacct 
      ccgcttccc ccgaaaaaa tcaatgcct agcataaat 
      aacgaggaa accgccaaa tcaccacta atgcaaaag 
      aatcggacg ttatcgcca cgcaatact aatagtaca 
      acaccgatt agttcaaca aatgcggaa aagaaaggg 
      tcatttcag ccggttact acgaaattg gttccgtat 
      gagacggac gattcgagt tgttccgac gaatcgaat 
      cagtcgcca caggatgtg gagtataga gtttctacg 
      aaggcggca gtgggcgat tggaaggtt acttcaact 
      gaaccaacg acgcagagt aaaagttgg ggagagaaa 
      aaacaagat gggactgtt agcgaattg ttcaaaatg 
      tcccacagt gggtattcg gcacctgtt tcctcgtgg 
      aacggtact aggtctcag ctagataag gaaattagc 
      aacgaaagg agagaagac agaaagaga gctttgagt 
      gataattcg gaccaaggc aaagtcaaa catcaaaaa 
      ctgaataac tcctacaag tctaatcct ggttacaat 
      cccgtacaa gaatttcac aacactaag aactggagt 
      cagaacaac ggttacaag ccgttttat tcgggcaac 
      cggcaccgt cgtaatttc caccacaaa agctttcat 
      aaaaattac cgctttagg cagtga
    mRNA sequence
    • >TC002978 RA
      aagagctcg tagttgtcg cgctgccct tggggcgaa 
      ttgacatgc gcactagtg acaacaagc tgaaaatcg 
      tcgttcgga gcaaattct ggtggtgcc actgatgtt 
      ctaaataaa tcgcgcaat tggcgttat ctgataaaa 
      aaccgcgtc gcgccaact cgcccccga gccagtgtt 
      cgagactat gctgcaccg acagccccc gagccccag 
      ctagcaccg ttcgaagcc agacaatac gggatgccg 
      gcgtccacc ctcgagccc gtgaacgcg gctttgcgc 
      tccgccctc accaagacc gacgacagt tctctggac 
      acgcagttg gcgggatcc accaagaag gccctcctg 
      tccaacatc gacttcgag ccggccggg agctaccaa 
      agcaccgtc ttggacaaa ctcaagtcg aagtacatc 
      gtgttgaaa agctcggag actccgcag cccagcaag 
      cctgaggga gacatgggc gcggatgaa acgctcaaa 
      ttggctagt tacgagctg tatcctttt gaagctgtg 
      cagttaggt tggagtaaa tgtgactgg tcagtgggg 
      gccggcatg attaacatg ggaaatacc tgttatctc 
      aactctacg cttcaagca cttttccac gttcccgct 
      cttgtcaat tggctcatt tctgataag gaacacaca 
      gctgaatgc caggactca ggtgggctg tgcataata 
      tgtgcaatg cggaagact ctccaagac tcccaacaa 
      cgtaacaca aacacaatc cgtcctctc ctcatctac 
      aacaagctg cgtttggtg tgtcgaaac ctcatcccg 
      ggtcggcaa gaagatgcg cacgaattt cttagatat 
      ctagtggaa gcaatggaa aaatcctat ttaagtcga 
      tttaaaaac cattccgaa tttgattca aaagtgaaa 
      gagaccaca cctctcaat caaatacta ggcggatat 
      ttaagatcg gcagtgaga tgtttgaag tgtggtcac 
      gtcagcacc accttccaa cactttcaa gatttacta 
      ttagacatt aggaaagct caaacgctc gacgaggcg 
      ttggaaggt tacttctct agggaaaaa ttggacgat 
      gactcgtat cattgtcag tcttgtcag aaaaaagta 
      cctgcaaca aaacagttc tcccttgaa agagcccct 
      atggttctt tgtatacaa ctcaaacgg ttttctgtt 
      agcaataat aaaattact aaacatata cattttcga 
      caaagactg gatctaaca aggtatgcc agacatcgt 
      cccagtgtt ccactgatc tatagactt gttgcctta 
      gtcacacat atgggacct actgttaat tgtggccac 
      tacactgca gttgcccaa gcgccgtct ggtaatttt 
      taccagttt gatgatagt atggtgcgg gtgatatct 
      caccaagct gtgttcaat acaaacgca tacataatg 
      ctgtacgaa ttagagtca tctccattt accaaatca 
      aataacggg acaaacaac aagataaaa cctccgctt 
      tctcccgaa gcaaatact agcaacaac acatcaaaa 
      gcttcactg aacggtatc ggttttacg agcgacaaa 
      gtctacggc cccgaactg ccccccgac cgagtagaa 
      aagaaaatc aacggtgtt aaccccaca ccggtcact 
      aacggtaac ggaaatcac tcagagagt tccggaacg 
      agcgacagc gaaccggaa aatccgcaa aaaagtcca 
      caaaaagaa aacaaaccg gtggcgaca cctccgctt 
      cccccgaaa aaatcaatg cctagcata aataacgag 
      gaaaccgcc aaatcacca ctaatgcaa aagaatcgg 
      acgttatcg ccacgcaat actaatagt acaacaccg 
      attagttca acaaatgcg gaaaagaaa gggtcattt 
      cagccggtt actacgaaa ttggttccg tatgagacg 
      gacgattcg agttgttcc gacgaatcg aatcagtcg 
      ccacaggat gtggagtat agagtttct acgaaggcg 
      gcagtgggc gattggaag gttacttca actgaacca 
      acgacgcag agtaaaagt tggggagag aaaaaacaa 
      gatgggact gttagcgaa ttgttcaaa atgtcccac 
      agtgggtat tcggcacct gtttcctcg tggaacggt 
      actaggtct cagctagat aaggaaatt agcaacgaa 
      aggagagaa gacagaaag agagctttg agtgataat 
      tcggaccaa ggcaaagtc aaacatcaa aaactgaat 
      aactcctac aagtctaat cctggttac aatcccgta 
      caagaattt cacaacact aagaactgg agtcagaac 
      aacggttac aagccgttt tattcgggc aaccggcac 
      cgtcgtaat ttccaccac aaaagcttt cataaaaat 
      taccgcttt aggcagtga ttaatttta aggttgaat 
      atttttaca taggcaatt gaacttgta taattgtgc 
      atattatgt caccattgt ttagcataa ctgaatttg 
      tgaatttat aatgtaagt atttatatt tttaaacaa 
      actataatt cctaatttt gattgaaaa accgatgta 
      ataaagtag cgatagtat aattaacaa tgaaatctt 
      ttaggaagt ggtatttgt ttcattttt ttgagctta 
      atgttcaca gtgcattta aaatactgt attttttct 
      atttct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY