Status
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Gene information
General information
TC003434 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5148597..5156450
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003434
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09747

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003434 RA
      MLDRVNKDL LPMKAHYFL FNAGTGPVQ PFLSTYARQ 
      LGFSATIVG LIYTILPIS GMLAKPIVG AIADRFRCQ 
      KLMFLAAQL LTAAAFLAI NFSPEIPKD QKVHFACDG 
      DAVFDTSPL TKVEIDDCL VDQFQKTPG NVTCRMNCP 
      MEEQFWKVV VQYWNATEY QNYHPDNFE FTAFVIREK 
      TIRINNATY FGFKYVELD SGKTTKALC PCGKECHIS 
      TVCDITCDD YTLNSMVTQ TAVSDEDVY SKSQFWIFL 
      ILMIFGWVG QAVVVSVGD AICFQLLGD KPNRYGYQR 
      LFGALGWGI FSVIAGLMV DSLSEGSSK KNFESAFYL 
      SFAFLLLDF AVSSNLKNA QTKQSTSIL KDVGRLLVE 
      FRIVVFLVW CIAVGMCTA LMWQFLFFL IEDLAKNQG 
      CDSQEWIKT LEGIVQAIQ TLAGEMPFF FWSGKILKR 
      IGHINAMSL VLLVIGIRF ILYSVITNP WWFLPIEFL 
      NGLTFGLFY ACMTSYASV AAPPGTEAT MQGLVGAIF 
      EGVGVSLGS FVGGLIYDA YSGPWTFRL FGIGALITC 
      LLHAGVQFL IKRSDYEAR YTSPSEAFS ALYDDQQEM 
      TLVE
    CDS sequence
    • >TC003434 RA
      atgttggac cgagtgaat aaagatttg cttcctatg 
      aaagcgcat tattttctt tttaatgca gggaccggc 
      ccggtccaa ccgttcctc tccacatac gcacgacag 
      ctcggtttt tccgcaaca atcgtagga ctaatttac 
      acaattttg ccaataagt ggcatgctt gcaaaaccg 
      attgtgggt gcgatagct gaccggttc cgttgccaa 
      aaactgatg tttctggcc gctcagcta ctaacagct 
      gccgctttt ctagccatc aatttttca cctgaaatc 
      cccaaagac caaaaagtc cattttgcg tgcgacggt 
      gacgcagtt tttgatact tcaccgctc actaaagtc 
      gaaattgac gactgtctc gtggaccag ttccagaaa 
      acacccgga aacgttacg tgtcgaatg aactgtcca 
      atggaggag cagttttgg aaagtggtt gtccagtac 
      tggaacgcg acggaatac cagaactac catcctgat 
      aatttcgaa ttcactgcg ttcgtaatt cgggaaaaa 
      acaatcagg atcaacaat gccacttat ttcggtttt 
      aaatatgtg gaactggac agtggtaaa accaccaaa 
      gctttatgt ccctgtggg aaagagtgc cacatttcg 
      acggtttgt gacatcact tgtgatgac tacactctg 
      aacagtatg gtcactcaa actgcagtg agtgatgaa 
      gatgtttac agtaaatca caattttgg atttttctc 
      atactcatg atttttggt tgggtgggg caagctgta 
      gttgttagt gttggagat gccatctgt ttccaactg 
      ctgggcgat aaacccaat cggtacggc taccaacgc 
      ctgtttggg gccctaggt tggggaata ttcagcgtt 
      atagcgggc cttatggtg gactctctg agtgaaggt 
      tcctcaaag aagaatttt gagtctgcc ttttatctc 
      tcttttgcc tttttacta ctagatttc gctgtttct 
      agtaacctg aaaaatgct caaacgaag cagtccacc 
      agtattctc aaagatgtg ggccgtttg ttggtcgaa 
      ttccgcatt gtagttttc ctagtttgg tgcatagct 
      gtagggatg tgcacagcc ctcatgtgg cagttcctc 
      ttcttcctg atcgaagat cttgccaaa aatcaaggt 
      tgcgattcg caagagtgg atcaagact cttgaaggg 
      atcgttcag gctattcag acactggct ggagaaatg 
      ccgtttttc ttctggtcg gggaagatt ttgaaacgt 
      attggacac ataaatgca atgtcgttg gttcttctg 
      gtcattggg atcagattt attctctat tcggttata 
      actaatccg tggtggttt ttaccgatt gagtttctc 
      aacgggttg acttttggg ctgttttat gcctgcatg 
      acgagttat gccagtgtt gcagcgcca ccagggact 
      gaagcgaca atgcagggc ctggtgggc gccatcttc 
      gagggcgtg ggcgtctcg ttgggaagt ttcgtgggt 
      ggcctcatt tacgacgcc tacagcggc ccttggacc 
      ttcagactg ttcggcatc ggagccctt ataacatgt 
      ttgttgcat gctggtgtt caatttttg atcaaacga 
      tcagattat gaggctcgt tacacgtcg ccttccgag 
      gcctttagt gctctctat gacgatcag caagaaatg 
      accttagtc gaataa
    mRNA sequence
    • >TC003434 RA
      tttatcgtg tcattttac cgtcagttc tcacgttac 
      acaagtgaa tgcatgtta taatttaaa tattttagt 
      gataaggca ctcactttc gaagtccta ttaattcag 
      acctccgtt taaaaaaat taacatata cccatttaa 
      tgtttggta cttgaaaag acattcaaa caggcccag 
      aaaaaataa gaattcgaa aatgttgga ccgagtgaa 
      taaagattt gcttcctat gaaagcgca ttattttct 
      ttttaatgc agggaccgg cccggtcca accgttcct 
      ctccacata cgcacgaca gctcggttt ttccgcaac 
      aatcgtagg actaattta cacaatttt gccaataag 
      tggcatgct tgcaaaacc gattgtggg tgcgatagc 
      tgaccggtt ccgttgcca aaaactgat gtttctggc 
      cgctcagct actaacagc tgccgcttt tctagccat 
      caatttttc acctgaaat ccccaaaga ccaaaaagt 
      ccattttgc gtgcgacgg tgacgcagt ttttgatac 
      ttcaccgct cactaaagt cgaaattga cgactgtct 
      cgtggacca gttccagaa aacacccgg aaacgttac 
      gtgtcgaat gaactgtcc aatggagga gcagttttg 
      gaaagtggt tgtccagta ctggaacgc gacggaata 
      ccagaacta ccatcctga taatttcga attcactgc 
      gttcgtaat tcgggaaaa aacaatcag gatcaacaa 
      tgccactta tttcggttt taaatatgt ggaactgga 
      cagtggtaa aaccaccaa agctttatg tccctgtgg 
      gaaagagtg ccacatttc gacggtttg tgacatcac 
      ttgtgatga ctacactct gaacagtat ggtcactca 
      aactgcagt gagtgatga agatgttta cagtaaatc 
      acaattttg gatttttct catactcat gatttttgg 
      ttgggtggg gcaagctgt agttgttag tgttggaga 
      tgccatctg tttccaact gctgggcga taaacccaa 
      tcggtacgg ctaccaacg cctgtttgg ggccctagg 
      ttggggaat attcagcgt tatagcggg ccttatggt 
      ggactctct gagtgaagg ttcctcaaa gaagaattt 
      tgagtctgc cttttatct ctcttttgc ctttttact 
      actagattt cgctgtttc tagtaacct gaaaaatgc 
      tcaaacgaa gcagtccac cagtattct caaagatgt 
      gggccgttt gttggtcga attccgcat tgtagtttt 
      cctagtttg gtgcatagc tgtagggat gtgcacagc 
      cctcatgtg gcagttcct cttcttcct gatcgaaga 
      tcttgccaa aaatcaagg ttgcgattc gcaagagtg 
      gatcaagac tcttgaagg gatcgttca ggctattca 
      gacactggc tggagaaat gccgttttt cttctggtc 
      ggggaagat tttgaaacg tattggaca cataaatgc 
      aatgtcgtt ggttcttct ggtcattgg gatcagatt 
      tattctcta ttcggttat aactaatcc gtggtggtt 
      tttaccgat tgagtttct caacgggtt gacttttgg 
      gctgtttta tgcctgcat gacgagtta tgccagtgt 
      tgcagcgcc accagggac tgaagcgac aatgcaggg 
      cctggtggg cgccatctt cgagggcgt gggcgtctc 
      gttgggaag tttcgtggg tggcctcat ttacgacgc 
      ctacagcgg cccttggac cttcagact gttcggcat 
      cggagccct tataacatg tttgttgca tgctggtgt 
      tcaattttt gatcaaacg atcagatta tgaggctcg 
      ttacacgtc gccttccga ggcctttag tgctctcta 
      tgacgatca gcaagaaat gaccttagt cgaataaca 
      gcatggacg aagcgacca attcagcct taagcaacg 
      aaaaatacg catttacgc gttacttaa cgttggatt 
      gtgcaaaca ttttgtgat agctactta aatccctgc 
      cctaatcgg acacatcag tggtaaatt ccaccaaat 
      tatcgtctt ccttgaccc acatttgga caaacattg 
      atgtttccg ataagtctt gaatattaa gatacgatt 
      ttagtaaaa gaatgacga aatttaaaa aaccaggct 
      cagggatca taacttaag ccggcttaa gttacagac 
      gaatttatt taatccttt tattactta agtattatt 
      gtttcccga ctgtcctcg agttaacct tcaccaatc 
      acgaaaggt tattctgcg tttacttgt ggttcttgt 
      cgttaactt gggaattct tttagagaa tcgaacagg 
      gcattaaaa aatttagat tttttacaa aaaaattaa 
      attattgtg atatcttgt aaagttatt tattagcgc 
      aatttacta acaagttat cagtatttt ggttcctcg 
      aattaattt acttaccaa ttgtatgta ttatggtct 
      cttttgtac actttttaa tttattgtt tgttactta 
      cctttgtta cgtatctta tattactta cctgttgta 
      acaattatg tttttataa tttgtatat tttgttccg 
      tggttttgt gcgtagcaa gtcaataaa tttgttttt 
      attaaagtg tgttatttt ttacctttt caccaaaaa 
      aattggatg aaatccaaa aaaatcaca aacaaatat 
      aaatcagag gagaaatag ttcgacgag atcagcagt 
      caacccaca aagttccta ttaaacaca tgattacaa 
      aatcatcga tatttttaa tgcagttcg atacttact 
      atgcgaagg ctacgactg gactgacga aagtgttaa 
      aaaattata caacggtgg tatttatca gcgaaccgt 
      ttgtctcag ataagattt aatgtaatt gtatgatgt 
      aaattttat gacgctatt actgcgcga ctttcaagt 
      tttatttga gagataaca aactgcatt atattagtt 
      aaaacctga aatgttttt tttaattta attatttat 
      ttgaaatcg ctcaataat taaaatatt gcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY