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Gene information
General information
TC003417 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4783101..4786362
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003417
    iBeetle screen iB_09745

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003417 RA
      MSDLNIGVL GAGVIGLTT AFELKKKFR NANIDVISH 
      QFNDKTTSY VAAGLFRPG TSFSGPTVE ITRQWINDS 
      YHYWDNIWN TPEASTAGV AQVSGYIFS SQYPNIVKN 
      DYLDKLLPI YRRATEDEL QLCPGTWKY GCFFTTVLT 
      QCSYYLPWI TSKLQRDGV HILSQKIES FAQLDKKYD 
      IILNCTGLG AKFLCNDNK LVPMRGQVL KVRAPWIKT 
      FFYGDYDTY VIPGIDCVT LGGCRQYDS YNLEVNKYD 
      GLSIKERCE SLVPSLRGA ELIAEKVGL RPHRDVVRV 
      EKEIQVING RKVKIVHNY GHGGYGVTT APGTSLYAV 
      KLATELLSG NSKL
    CDS sequence
    • >TC003417 RA
      atgtcggac ctcaatatt ggtgtatta ggagccggt 
      gtgattggt ttaacaaca gcgttcgaa ttgaaaaag 
      aaattccga aacgcaaac attgatgtt atttcccat 
      cagttcaat gacaagacc accagttac gtagcagct 
      ggacttttc cgtccaggg actagtttt tcaggcccc 
      acggtggaa atcacaaga caatggatc aatgattct 
      tatcactac tgggataac atttggaac acacctgag 
      gcaagcaca gctggtgtt gcccaagtg tcgggctac 
      attttctcc agtcagtac ccaaatatc gtcaaaaac 
      gattatctc gacaagtta ttgcccatt tatcgacgg 
      gccactgaa gatgaatta caattatgt cccgggacg 
      tggaaatac ggctgtttt ttcacaaca gtcctcacc 
      caatgctcg tactacttg ccctggatc acttcaaaa 
      ctgcaaagg gacggcgtc cacattctg agccaaaaa 
      atcgagtcg tttgcacaa ctcgataaa aagtacgac 
      ataatctta aactgcacc ggccttggg gcgaaattt 
      ctgtgcaat gacaataaa ctcgtgcca atgcgaggc 
      caagtcctg aaagtgagg gctccttgg attaaaaca 
      tttttctac ggggattac gacacttat gttattccg 
      ggcattgac tgtgtgact ttgggcggt tgtcgtcag 
      tacgactcg tataacttg gaagtgaat aaatacgac 
      ggtttgtcg atcaaagaa cggtgcgag tctctggtg 
      ccaagtctg agaggtgcc gaattaatc gcggaaaaa 
      gtgggcctg agaccgcac agagatgtg gtcagggtg 
      gagaaggaa attcaggtg attaacggc aggaaagtg 
      aaaattgtg cacaattac gggcacggt ggctatggg 
      gttaccaca gctccgggg acgtcgctg tatgccgtg 
      aaactagcg actgagttg ctttcaggc aacagcaag 
      ttataa
    mRNA sequence
    • >TC003417 RA
      ggcggagct acaaataaa tgttatctt attatcaca 
      attctaatt cagttgtga caattaaac aaaggtcat 
      catgtttac ataaaagcc tggagataa acacttaac 
      attcattgt gactaacaa attttctgc acgaaaatg 
      tcggacctc aatattggt gtattagga gccggtgtg 
      attggttta acaacagcg ttcgaattg aaaaagaaa 
      ttccgaaac gcaaacatt gatgttatt tcccatcag 
      ttcaatgac aagaccacc agttacgta gcagctgga 
      cttttccgt ccagggact agtttttca ggccccacg 
      gtggaaatc acaagacaa tggatcaat gattcttat 
      cactactgg gataacatt tggaacaca cctgaggca 
      agcacagct ggtgttgcc caagtgtcg ggctacatt 
      ttctccagt cagtaccca aatatcgtc aaaaacgat 
      tatctcgac aagttattg cccatttat cgacgggcc 
      actgaagat gaattacaa ttatgtccc gggacgtgg 
      aaatacggc tgttttttc acaacagtc ctcacccaa 
      tgctcgtac tacttgccc tggatcact tcaaaactg 
      caaagggac ggcgtccac attctgagc caaaaaatc 
      gagtcgttt gcacaactc gataaaaag tacgacata 
      atcttaaac tgcaccggc cttggggcg aaatttctg 
      tgcaatgac aataaactc gtgccaatg cgaggccaa 
      gtcctgaaa gtgagggct ccttggatt aaaacattt 
      ttctacggg gattacgac acttatgtt attccgggc 
      attgactgt gtgactttg ggcggttgt cgtcagtac 
      gactcgtat aacttggaa gtgaataaa tacgacggt 
      ttgtcgatc aaagaacgg tgcgagtct ctggtgcca 
      agtctgaga ggtgccgaa ttaatcgcg gaaaaagtg 
      ggcctgaga ccgcacaga gatgtggtc agggtggag 
      aaggaaatt caggtgatt aacggcagg aaagtgaaa 
      attgtgcac aattacggg cacggtggc tatggggtt 
      accacagct ccggggacg tcgctgtat gccgtgaaa 
      ctagcgact gagttgctt tcaggcaac agcaagtta 
      taagactcc gaaagataa atagttata tttttaagt 
      aataaaata tcaattagt ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY