Status
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Gene information
General information
TC003021 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4316322..4323510
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003021
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09742

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003021 RA
      MSSTLDPAK LKVVELRAE LSKRGLDSK GNKPALVKR 
      LKEALEEEL KQELPDTSI ADTSTEDLD TSQTKNDSV 
      TEESKVPDK EPEKTEACP LPPKEQPPV EQPEATTEE 
      KMDTQEAPP AEPVQQPAP EVEAPKPEE TEPSEQSEP 
      KELNSNLEE NKENGEQDE PKSRKSRWG GSETDGSEA 
      PPKEEVENN QAESTEAPV SENKDGEAK GEKRRRSSP 
      SPDRSQRRR SKSPIKEDE PPIDKDKVQ LSWYDSDLH 
      LQVDKESFL SAKPYHEGA FGFAWAGVR ATHGVNTGK 
      VCFEVKVTE ELKWEDFSK YYDKHRREQ HKSSRHKDD 
      DKKKSDESK SKDEQSKDK AESSQENGE KSETTDPDS 
      ETKETSVEN DSEMKETAD SENEDNKKS ADENPPEEN 
      PQEEPMETE ESKEKSDEK DESETKETE PIPTHLFRV 
      GWSLLDTGL QLGEDKFSY GYESSGRFA LNKQFEDYG 
      KKFGVGDVV ASFLEITEA EIKISYSVN GEMQGDAIS 
      VPCSDLPEN FVFFPHVLS RNYAFELNL GNKEEAWFS 
      KPEGFEDFE FLANVEEKV AGPARPENR SDCEVILMC 
      GLPAAGKTH WVQEYVQEN PEKRYTVLG NTHLLQKMT 
      VSGDELKNK YKGSWNMLL EKLQKCLNK LVEVAALRR 
      RNYIIDQPN VYPSAQRRK LRPFEGFKR RAVVVVVGD 
      EEQAKRQSL QEAQGGKDV PDSTVLDMK ANMSLPQKG 
      EWIDEVLYV GLGEEEAKS LVQKYNSQA RETIGVQRR 
      FGHRDDRHR WQYRRNDYR GSNYRDRSY HSNRYDRGP 
      PRQGNWHPQ RPGGWNRDR RDNRGPPPR DWRNRDRGS 
      RDSHQGSNY NRNRNQVGR GPGGNWPSS WGGQSQGSW 
      SNQPWSQGS WGQGGWNNQ SSQNQWKYG GGSQSYGYS 
      NWNYYGQYP QNWSGTQGQ SAGSNTAGA NQYGQYSQQ 
      DWAQYAQQY AQQGQTTTG STATQSTPQ K
    • >TC003021 RB
      MSSTLDPAK LKVVELRAE LSKRGLDSK GNKPALVKR 
      LKEALEEEL KQELPDTSI ADTSTEDLD TSQTKNDSV 
      TEESKVPDK EPEKTEACP LPPKEQPPV EQPEATTEE 
      KMDTQEAPP AEPVQQPAP EVEAPKPEE TEPSEQSEP 
      KELNSNLEE NKENGEQDE PKSRKSRWG GSETDGSEA 
      PPKEEVENN QAESTEAPV SENKDGEAK GEKRRRSSP 
      SPDRSQRRR SKSPIKEDE PPIDKDKVQ LSWYDSDLH 
      LQVDKESFL SAKPYHEGA FGFAWAGVR ATHGVNTGK 
      VCFEVKVTE ELKWEDFSK YYDKHRREQ HKSSRHKDD 
      DKKKSDESK SKDEQSKDK AESSQENGE KSETTDPDS 
      ETKETSVEN DSEMKETAD SENEDNKKS ADENPPEEN 
      PQEEPMETE ESKEKSDEK DESETKETE PIPTHLFRV 
      GWSLLDTGL QLGEDKFSY GYESSGRFA LNKQFEDYG 
      KKFGVGDVV ASFLEITEA EIKISYSVN GEMQGDAIS 
      VPCSDLPEN FVFFPHVLS RNYAFELNL GNKEEAWFS 
      KPEGFEDFE FLANVEEKV AGPARPENR SDCEVILMC 
      GLPAAGKTH WVQEYVQEN PEKRYTVLG NTHLLQKMT 
      VSGDELKNK YKGSWNMLL EKLQKCLNK LVEVAALRR 
      RNYIIDQPN VYPSAQRRK LRPFEGFKR RAVVVVVGD 
      EEQAKRQSL QEAQGGKDV PDSTVLDMK ANMSLPQKG 
      EWIDEVLYV GLGEEEAKS LVQKYNSQA RETIGVQRR 
      FGHRDDRHR WQYRRNDYR GSNYRDRSY HSNRYDRGP 
      PRQGNWHPQ RPGGWNRDR RDNRGPPPR DWRNRGNSR 
      DRGSRDSHQ GSNYNRNRN QVGRGPGGN WPSSWGGQS 
      QGSWSNQPW SQGSWGQGG WNNQSSQNQ WKYGGGSQS 
      YGYSNWNYY GQYPQNWSG TQGQSAGSN TAGANQYGQ 
      YSQQDWAQY AQQYAQQGQ TTTGSTATQ STPQK
    CDS sequence
    • >TC003021 RA
      atgagttca acactggac cctgccaag ttaaaagtg 
      gtagaatta cgtgcagaa ctctcaaag cgtggtttg 
      gattcaaaa ggaaacaaa ccggcgtta gtcaagcgc 
      ttgaaagaa gccttggag gaagaactc aaacaagag 
      ttaccagat acttcaatt gctgatacg tcgacggaa 
      gatttggat acttcgcaa actaaaaat gattccgtt 
      accgaagaa tcgaaagta cctgataag gaacctgaa 
      aaaaccgaa gcctgtcca ctaccacca aaagaacaa 
      ccaccagtg gagcaacca gaagccaca acagaagaa 
      aaaatggat acacaagaa gcgcctcct gcagaaccc 
      gtacaacaa cctgcacct gaagttgaa gcacctaaa 
      ccagaagaa accgaacct tctgaacag tctgaacca 
      aaagaactt aattccaat ttagaagag aataaggaa 
      aacggggaa caggatgaa cccaagtcg cggaaatca 
      cgctggggt ggttctgaa accgacggt tctgaagct 
      ccgccaaag gaggaggtt gagaacaac caagctgaa 
      agcactgaa gcaccagtc agcgaaaat aaagacggt 
      gaagccaag ggcgaaaag cgaagacgt agtagtcca 
      tctccggac agatctcaa cgccgacga agtaaatcg 
      ccaattaaa gaagacgag cctcctata gataaggac 
      aaagttcag cttagctgg tatgactct gaccttcat 
      ctgcaagtt gataaagaa tcgttctta tcagcaaaa 
      ccctatcat gaaggtgct ttcggtttt gcatgggcg 
      ggagtcaga gcgacccat ggcgtcaat actggaaag 
      gtgtgtttc gaagttaaa gtaacggaa gaactcaaa 
      tgggaggat ttttccaaa tactacgac aaacaccgt 
      cgagaacaa cataaatct tcgagacac aaagatgac 
      gataaaaag aaatctgac gagtcgaaa agtaaagac 
      gagcaaagt aaagacaag gctgagagc agccaagaa 
      aacggcgaa aagtcggaa accactgat cccgactct 
      gaaactaaa gaaacgagc gtcgagaac gactcagaa 
      atgaaggaa actgccgac agtgaaaac gaagataat 
      aaaaagagt gccgacgag aacccacca gaggaaaat 
      ccacaagaa gaaccaatg gaaactgag gaaagtaaa 
      gaaaaatca gacgagaaa gacgaatcg gaaaccaaa 
      gaaactgaa cccataccc acccacttg ttccgagtc 
      gggtggtcg ctcctagac actggactc cagctagga 
      gaggacaag ttttcttac ggttatgag tcatctgga 
      agattcgcg ctcaataag cagtttgaa gattacggg 
      aaaaagttc ggcgttggc gacgttgtt gctagtttt 
      ttggaaatc actgaggct gagatcaaa atttcttac 
      tccgtcaat ggtgagatg caaggagac gcgatatcc 
      gtcccatgt tccgacctc ccggaaaat ttcgtcttt 
      ttcccccac gtcctttcc cgcaattac gcgtttgaa 
      ttgaatctg ggtaataaa gaagaggcg tggttcagc 
      aaaccggag ggttttgaa gattttgag tttttggcc 
      aatgtggag gagaaggtt gctgggcct gcaaggccc 
      gaaaataga agcgattgc gaggtgatt ctgatgtgt 
      gggttaccg gctgctggg aaaacgcac tgggttcaa 
      gaatatgtc caagagaat ccggagaag agatacacc 
      gtcttgggc aatacacat ctgttgcaa aaaatgact 
      gtttccggc gatgaattg aaaaataaa tacaaaggg 
      agttggaac atgctgctg gagaagctt caaaaatgt 
      ttgaacaag ctggtggaa gtggctgcc cttcgccga 
      agaaactac attattgat cagccaaac gtgtaccca 
      tcagcccag cgccgcaaa ttgcggcca tttgagggc 
      ttcaagagg cgtgctgtc gttgttgta gtaggagat 
      gaagaacaa gctaaacgg caatctttg caagaggct 
      cagggcggt aaagacgtt cccgatagc accgtgctt 
      gatatgaaa gcaaacatg agcttacca cagaagggg 
      gaatggatt gacgaagta ctctatgta ggccttggc 
      gaagaagaa gctaaatcc ctggttcag aagtataat 
      tcccaagcg cgcgaaaca attggcgtg caaagaaga 
      tttggacat cgagatgac cgccataga tggcagtac 
      agacgcaat gattaccgg gggagtaac taccgagac 
      aggagttac cacagcaac cgctacgac agaggcccc 
      ccgagacag ggcaactgg cacccgcaa cgtcccgga 
      ggatggaac agagacaga cgggacaac cgagggccc 
      cctccgaga gattggagg aacagagac cgcggcagc 
      cgagactcg caccagggc agcaactac aaccgcaat 
      cgcaaccaa gttggacgg gggcctggg ggcaactgg 
      ccctccagc tggggcggc cagagccag ggcagctgg 
      agcaaccag ccgtggagt cagggctcc tggggccag 
      ggcggttgg aacaaccag agcagccaa aaccagtgg 
      aagtacgga ggagggagt cagagttac ggctactcc 
      aactggaat tactacggg cagtatccc cagaactgg 
      agcggcact cagggccaa tctgctggt agtaataca 
      gcgggggca aaccagtac ggtcagtac tcccagcag 
      gattgggcg caatatgca caacaatac gcccagcaa 
      gggcagacc acaaccggg tcaaccgcc actcagtcc 
      actcctcaa aagtaa
    • >TC003021 RB
      atgagttca acactggac cctgccaag ttaaaagtg 
      gtagaatta cgtgcagaa ctctcaaag cgtggtttg 
      gattcaaaa ggaaacaaa ccggcgtta gtcaagcgc 
      ttgaaagaa gccttggag gaagaactc aaacaagag 
      ttaccagat acttcaatt gctgatacg tcgacggaa 
      gatttggat acttcgcaa actaaaaat gattccgtt 
      accgaagaa tcgaaagta cctgataag gaacctgaa 
      aaaaccgaa gcctgtcca ctaccacca aaagaacaa 
      ccaccagtg gagcaacca gaagccaca acagaagaa 
      aaaatggat acacaagaa gcgcctcct gcagaaccc 
      gtacaacaa cctgcacct gaagttgaa gcacctaaa 
      ccagaagaa accgaacct tctgaacag tctgaacca 
      aaagaactt aattccaat ttagaagag aataaggaa 
      aacggggaa caggatgaa cccaagtcg cggaaatca 
      cgctggggt ggttctgaa accgacggt tctgaagct 
      ccgccaaag gaggaggtt gagaacaac caagctgaa 
      agcactgaa gcaccagtc agcgaaaat aaagacggt 
      gaagccaag ggcgaaaag cgaagacgt agtagtcca 
      tctccggac agatctcaa cgccgacga agtaaatcg 
      ccaattaaa gaagacgag cctcctata gataaggac 
      aaagttcag cttagctgg tatgactct gaccttcat 
      ctgcaagtt gataaagaa tcgttctta tcagcaaaa 
      ccctatcat gaaggtgct ttcggtttt gcatgggcg 
      ggagtcaga gcgacccat ggcgtcaat actggaaag 
      gtgtgtttc gaagttaaa gtaacggaa gaactcaaa 
      tgggaggat ttttccaaa tactacgac aaacaccgt 
      cgagaacaa cataaatct tcgagacac aaagatgac 
      gataaaaag aaatctgac gagtcgaaa agtaaagac 
      gagcaaagt aaagacaag gctgagagc agccaagaa 
      aacggcgaa aagtcggaa accactgat cccgactct 
      gaaactaaa gaaacgagc gtcgagaac gactcagaa 
      atgaaggaa actgccgac agtgaaaac gaagataat 
      aaaaagagt gccgacgag aacccacca gaggaaaat 
      ccacaagaa gaaccaatg gaaactgag gaaagtaaa 
      gaaaaatca gacgagaaa gacgaatcg gaaaccaaa 
      gaaactgaa cccataccc acccacttg ttccgagtc 
      gggtggtcg ctcctagac actggactc cagctagga 
      gaggacaag ttttcttac ggttatgag tcatctgga 
      agattcgcg ctcaataag cagtttgaa gattacggg 
      aaaaagttc ggcgttggc gacgttgtt gctagtttt 
      ttggaaatc actgaggct gagatcaaa atttcttac 
      tccgtcaat ggtgagatg caaggagac gcgatatcc 
      gtcccatgt tccgacctc ccggaaaat ttcgtcttt 
      ttcccccac gtcctttcc cgcaattac gcgtttgaa 
      ttgaatctg ggtaataaa gaagaggcg tggttcagc 
      aaaccggag ggttttgaa gattttgag tttttggcc 
      aatgtggag gagaaggtt gctgggcct gcaaggccc 
      gaaaataga agcgattgc gaggtgatt ctgatgtgt 
      gggttaccg gctgctggg aaaacgcac tgggttcaa 
      gaatatgtc caagagaat ccggagaag agatacacc 
      gtcttgggc aatacacat ctgttgcaa aaaatgact 
      gtttccggc gatgaattg aaaaataaa tacaaaggg 
      agttggaac atgctgctg gagaagctt caaaaatgt 
      ttgaacaag ctggtggaa gtggctgcc cttcgccga 
      agaaactac attattgat cagccaaac gtgtaccca 
      tcagcccag cgccgcaaa ttgcggcca tttgagggc 
      ttcaagagg cgtgctgtc gttgttgta gtaggagat 
      gaagaacaa gctaaacgg caatctttg caagaggct 
      cagggcggt aaagacgtt cccgatagc accgtgctt 
      gatatgaaa gcaaacatg agcttacca cagaagggg 
      gaatggatt gacgaagta ctctatgta ggccttggc 
      gaagaagaa gctaaatcc ctggttcag aagtataat 
      tcccaagcg cgcgaaaca attggcgtg caaagaaga 
      tttggacat cgagatgac cgccataga tggcagtac 
      agacgcaat gattaccgg gggagtaac taccgagac 
      aggagttac cacagcaac cgctacgac agaggcccc 
      ccgagacag ggcaactgg cacccgcaa cgtcccgga 
      ggatggaac agagacaga cgggacaac cgagggccc 
      cctccgaga gattggagg aacagaggt aattctcga 
      gaccgcggc agccgagac tcgcaccag ggcagcaac 
      tacaaccgc aatcgcaac caagttgga cgggggcct 
      gggggcaac tggccctcc agctggggc ggccagagc 
      cagggcagc tggagcaac cagccgtgg agtcagggc 
      tcctggggc cagggcggt tggaacaac cagagcagc 
      caaaaccag tggaagtac ggaggaggg agtcagagt 
      tacggctac tccaactgg aattactac gggcagtat 
      ccccagaac tggagcggc actcagggc caatctgct 
      ggtagtaat acagcgggg gcaaaccag tacggtcag 
      tactcccag caggattgg gcgcaatat gcacaacaa 
      tacgcccag caagggcag accacaacc gggtcaacc 
      gccactcag tccactcct caaaagtaa
    mRNA sequence
    • >TC003021 RA
      ccttttttg gcaaaaagt agttcaaag aggatgtat 
      gtgaaagta agcacgtta agataggtg ggcatgggt 
      gccacaagt taaattctg aatgagtta cttatgata 
      agaaaccaa aattttgat ttattcttt tcttttttt 
      ctaaattca aatgtaaat ggcgggaaa gtcggagaa 
      agcgtacgc atgcgttgc cgaaccaac gcatcacgt 
      ccgccattt gcaaaacta gcgcgtgct ttctgcgtg 
      tgtgtgtta atattttgt gtttagatt tttactgtg 
      aactgctga aaatctagt agaaaaatg agttcaaca 
      ctggaccct gccaagtta aaagtggta gaattacgt 
      gcagaactc tcaaagcgt ggtttggat tcaaaagga 
      aacaaaccg gcgttagtc aagcgcttg aaagaagcc 
      ttggaggaa gaactcaaa caagagtta ccagatact 
      tcaattgct gatacgtcg acggaagat ttggatact 
      tcgcaaact aaaaatgat tccgttacc gaagaatcg 
      aaagtacct gataaggaa cctgaaaaa accgaagcc 
      tgtccacta ccaccaaaa gaacaacca ccagtggag 
      caaccagaa gccacaaca gaagaaaaa atggataca 
      caagaagcg cctcctgca gaacccgta caacaacct 
      gcacctgaa gttgaagca cctaaacca gaagaaacc 
      gaaccttct gaacagtct gaaccaaaa gaacttaat 
      tccaattta gaagagaat aaggaaaac ggggaacag 
      gatgaaccc aagtcgcgg aaatcacgc tggggtggt 
      tctgaaacc gacggttct gaagctccg ccaaaggag 
      gaggttgag aacaaccaa gctgaaagc actgaagca 
      ccagtcagc gaaaataaa gacggtgaa gccaagggc 
      gaaaagcga agacgtagt agtccatct ccggacaga 
      tctcaacgc cgacgaagt aaatcgcca attaaagaa 
      gacgagcct cctatagat aaggacaaa gttcagctt 
      agctggtat gactctgac cttcatctg caagttgat 
      aaagaatcg ttcttatca gcaaaaccc tatcatgaa 
      ggtgctttc ggttttgca tgggcggga gtcagagcg 
      acccatggc gtcaatact ggaaaggtg tgtttcgaa 
      gttaaagta acggaagaa ctcaaatgg gaggatttt 
      tccaaatac tacgacaaa caccgtcga gaacaacat 
      aaatcttcg agacacaaa gatgacgat aaaaagaaa 
      tctgacgag tcgaaaagt aaagacgag caaagtaaa 
      gacaaggct gagagcagc caagaaaac ggcgaaaag 
      tcggaaacc actgatccc gactctgaa actaaagaa 
      acgagcgtc gagaacgac tcagaaatg aaggaaact 
      gccgacagt gaaaacgaa gataataaa aagagtgcc 
      gacgagaac ccaccagag gaaaatcca caagaagaa 
      ccaatggaa actgaggaa agtaaagaa aaatcagac 
      gagaaagac gaatcggaa accaaagaa actgaaccc 
      atacccacc cacttgttc cgagtcggg tggtcgctc 
      ctagacact ggactccag ctaggagag gacaagttt 
      tcttacggt tatgagtca tctggaaga ttcgcgctc 
      aataagcag tttgaagat tacgggaaa aagttcggc 
      gttggcgac gttgttgct agttttttg gaaatcact 
      gaggctgag atcaaaatt tcttactcc gtcaatggt 
      gagatgcaa ggagacgcg atatccgtc ccatgttcc 
      gacctcccg gaaaatttc gtctttttc ccccacgtc 
      ctttcccgc aattacgcg tttgaattg aatctgggt 
      aataaagaa gaggcgtgg ttcagcaaa ccggagggt 
      tttgaagat tttgagttt ttggccaat gtggaggag 
      aaggttgct gggcctgca aggcccgaa aatagaagc 
      gattgcgag gtgattctg atgtgtggg ttaccggct 
      gctgggaaa acgcactgg gttcaagaa tatgtccaa 
      gagaatccg gagaagaga tacaccgtc ttgggcaat 
      acacatctg ttgcaaaaa atgactgtt tccggcgat 
      gaattgaaa aataaatac aaagggagt tggaacatg 
      ctgctggag aagcttcaa aaatgtttg aacaagctg 
      gtggaagtg gctgccctt cgccgaaga aactacatt 
      attgatcag ccaaacgtg tacccatca gcccagcgc 
      cgcaaattg cggccattt gagggcttc aagaggcgt 
      gctgtcgtt gttgtagta ggagatgaa gaacaagct 
      aaacggcaa tctttgcaa gaggctcag ggcggtaaa 
      gacgttccc gatagcacc gtgcttgat atgaaagca 
      aacatgagc ttaccacag aagggggaa tggattgac 
      gaagtactc tatgtaggc cttggcgaa gaagaagct 
      aaatccctg gttcagaag tataattcc caagcgcgc 
      gaaacaatt ggcgtgcaa agaagattt ggacatcga 
      gatgaccgc catagatgg cagtacaga cgcaatgat 
      taccggggg agtaactac cgagacagg agttaccac 
      agcaaccgc tacgacaga ggccccccg agacagggc 
      aactggcac ccgcaacgt cccggagga tggaacaga 
      gacagacgg gacaaccga gggccccct ccgagagat 
      tggaggaac agagaccgc ggcagccga gactcgcac 
      cagggcagc aactacaac cgcaatcgc aaccaagtt 
      ggacggggg cctgggggc aactggccc tccagctgg 
      ggcggccag agccagggc agctggagc aaccagccg 
      tggagtcag ggctcctgg ggccagggc ggttggaac 
      aaccagagc agccaaaac cagtggaag tacggagga 
      gggagtcag agttacggc tactccaac tggaattac 
      tacgggcag tatccccag aactggagc ggcactcag 
      ggccaatct gctggtagt aatacagcg ggggcaaac 
      cagtacggt cagtactcc cagcaggat tgggcgcaa 
      tatgcacaa caatacgcc cagcaaggg cagaccaca 
      accgggtca accgccact cagtccact cctcaaaag 
      taaatgtat tgcctacat taacgccta gtattttat 
      gaatagtac ctttattgt tcaggtttt agatttaac 
      accacgtag cagttgtac agagcggca gcgctgcgt 
      tgccatttg tgcaccgat tttcatttt ttaatgctt 
      taacgtttc tttcgtgca attaagtta tattctttc 
      attgactat tttattcgc acgcaaatt gaccatatt 
      caaagttta tttttataa ttttatgta tatcggagt 
      gaagtttaa ttgatttgc ttctgtaaa taaagtttt 
      atgttcaaa acattcaaa gtttattta gaaatatgt 
      atatttttg gattcgcct catggtaaa ataactgaa 
      taggtgcgc tggtgtttg tcataataa ataattttt 
      attccattt tatcaaagt tcagatatt gtaccatgg 
      aataatttt tggggttga gtcaagggc tccaatgtg 
      gtaatttta aattattta tactgtaca taacattaa 
      agtacaaca atttttatg tcgagtctg ttgtaagta 
      ttacttatt ttcactttc ttaacttta tcgtagttt 
      ataaaggcc cgtctgaca tggcgcagt aaattgatt 
      aatttgttt gaacattat tgaataggt gtcattttc 
      taagcaaac gtgtcacgg ggggcgatg cccaaaatt 
      atttcttga agtaagagt agtgttgag tgtaatata 
      agaaatatt acaaacatg atgtattta ctttagtta 
      aaaaaataa atatatgat tttgta
    • >TC003021 RB
      ccttttttg gcaaaaagt agttcaaag aggatgtat 
      gtgaaagta agcacgtta agataggtg ggcatgggt 
      gccacaagt taaattctg aatgagtta cttatgata 
      agaaaccaa aattttgat ttattcttt tcttttttt 
      ctaaattca aatgtaaat ggcgggaaa gtcggagaa 
      agcgtacgc atgcgttgc cgaaccaac gcatcacgt 
      ccgccattt gcaaaacta gcgcgtgct ttctgcgtg 
      tgtgtgtta atattttgt gtttagatt tttactgtg 
      aactgctga aaatctagt agaaaaatg agttcaaca 
      ctggaccct gccaagtta aaagtggta gaattacgt 
      gcagaactc tcaaagcgt ggtttggat tcaaaagga 
      aacaaaccg gcgttagtc aagcgcttg aaagaagcc 
      ttggaggaa gaactcaaa caagagtta ccagatact 
      tcaattgct gatacgtcg acggaagat ttggatact 
      tcgcaaact aaaaatgat tccgttacc gaagaatcg 
      aaagtacct gataaggaa cctgaaaaa accgaagcc 
      tgtccacta ccaccaaaa gaacaacca ccagtggag 
      caaccagaa gccacaaca gaagaaaaa atggataca 
      caagaagcg cctcctgca gaacccgta caacaacct 
      gcacctgaa gttgaagca cctaaacca gaagaaacc 
      gaaccttct gaacagtct gaaccaaaa gaacttaat 
      tccaattta gaagagaat aaggaaaac ggggaacag 
      gatgaaccc aagtcgcgg aaatcacgc tggggtggt 
      tctgaaacc gacggttct gaagctccg ccaaaggag 
      gaggttgag aacaaccaa gctgaaagc actgaagca 
      ccagtcagc gaaaataaa gacggtgaa gccaagggc 
      gaaaagcga agacgtagt agtccatct ccggacaga 
      tctcaacgc cgacgaagt aaatcgcca attaaagaa 
      gacgagcct cctatagat aaggacaaa gttcagctt 
      agctggtat gactctgac cttcatctg caagttgat 
      aaagaatcg ttcttatca gcaaaaccc tatcatgaa 
      ggtgctttc ggttttgca tgggcggga gtcagagcg 
      acccatggc gtcaatact ggaaaggtg tgtttcgaa 
      gttaaagta acggaagaa ctcaaatgg gaggatttt 
      tccaaatac tacgacaaa caccgtcga gaacaacat 
      aaatcttcg agacacaaa gatgacgat aaaaagaaa 
      tctgacgag tcgaaaagt aaagacgag caaagtaaa 
      gacaaggct gagagcagc caagaaaac ggcgaaaag 
      tcggaaacc actgatccc gactctgaa actaaagaa 
      acgagcgtc gagaacgac tcagaaatg aaggaaact 
      gccgacagt gaaaacgaa gataataaa aagagtgcc 
      gacgagaac ccaccagag gaaaatcca caagaagaa 
      ccaatggaa actgaggaa agtaaagaa aaatcagac 
      gagaaagac gaatcggaa accaaagaa actgaaccc 
      atacccacc cacttgttc cgagtcggg tggtcgctc 
      ctagacact ggactccag ctaggagag gacaagttt 
      tcttacggt tatgagtca tctggaaga ttcgcgctc 
      aataagcag tttgaagat tacgggaaa aagttcggc 
      gttggcgac gttgttgct agttttttg gaaatcact 
      gaggctgag atcaaaatt tcttactcc gtcaatggt 
      gagatgcaa ggagacgcg atatccgtc ccatgttcc 
      gacctcccg gaaaatttc gtctttttc ccccacgtc 
      ctttcccgc aattacgcg tttgaattg aatctgggt 
      aataaagaa gaggcgtgg ttcagcaaa ccggagggt 
      tttgaagat tttgagttt ttggccaat gtggaggag 
      aaggttgct gggcctgca aggcccgaa aatagaagc 
      gattgcgag gtgattctg atgtgtggg ttaccggct 
      gctgggaaa acgcactgg gttcaagaa tatgtccaa 
      gagaatccg gagaagaga tacaccgtc ttgggcaat 
      acacatctg ttgcaaaaa atgactgtt tccggcgat 
      gaattgaaa aataaatac aaagggagt tggaacatg 
      ctgctggag aagcttcaa aaatgtttg aacaagctg 
      gtggaagtg gctgccctt cgccgaaga aactacatt 
      attgatcag ccaaacgtg tacccatca gcccagcgc 
      cgcaaattg cggccattt gagggcttc aagaggcgt 
      gctgtcgtt gttgtagta ggagatgaa gaacaagct 
      aaacggcaa tctttgcaa gaggctcag ggcggtaaa 
      gacgttccc gatagcacc gtgcttgat atgaaagca 
      aacatgagc ttaccacag aagggggaa tggattgac 
      gaagtactc tatgtaggc cttggcgaa gaagaagct 
      aaatccctg gttcagaag tataattcc caagcgcgc 
      gaaacaatt ggcgtgcaa agaagattt ggacatcga 
      gatgaccgc catagatgg cagtacaga cgcaatgat 
      taccggggg agtaactac cgagacagg agttaccac 
      agcaaccgc tacgacaga ggccccccg agacagggc 
      aactggcac ccgcaacgt cccggagga tggaacaga 
      gacagacgg gacaaccga gggccccct ccgagagat 
      tggaggaac agaggtaat tctcgagac cgcggcagc 
      cgagactcg caccagggc agcaactac aaccgcaat 
      cgcaaccaa gttggacgg gggcctggg ggcaactgg 
      ccctccagc tggggcggc cagagccag ggcagctgg 
      agcaaccag ccgtggagt cagggctcc tggggccag 
      ggcggttgg aacaaccag agcagccaa aaccagtgg 
      aagtacgga ggagggagt cagagttac ggctactcc 
      aactggaat tactacggg cagtatccc cagaactgg 
      agcggcact cagggccaa tctgctggt agtaataca 
      gcgggggca aaccagtac ggtcagtac tcccagcag 
      gattgggcg caatatgca caacaatac gcccagcaa 
      gggcagacc acaaccggg tcaaccgcc actcagtcc 
      actcctcaa aagtaaatg tattgccta cattaacgc 
      ctagtattt tatgaatag tacctttat tgttcaggt 
      tttagattt aacaccacg tagcagttg tacagagcg 
      gcagcgctg cgttgccat ttgtgcacc gattttcat 
      tttttaatg ctttaacgt ttctttcgt gcaattaag 
      ttatattct ttcattgac tattttatt cgcacgcaa 
      attgaccat attcaaagt ttattttta taattttat 
      gtatatcgg agtgaagtt taattgatt tgcttctgt 
      aaataaagt tttatgttc aaaacattc aaagtttat 
      ttagaaata tgtatattt ttggattcg cctcatggt 
      aaaataact gaataggtg cgctggtgt ttgtcataa 
      taaataatt tttattcca ttttatcaa agttcagat 
      attgtacca tggaataat ttttggggt tgagtcaag 
      ggctccaat gtggtaatt ttaaattat ttatactgt 
      acataacat taaagtaca acaattttt atgtcgagt 
      ctgttgtaa gtattactt attttcact ttcttaact 
      ttatcgtag tttataaag gcccgtctg acatggcgc 
      agtaaattg attaatttg tttgaacat tattgaata 
      ggtgtcatt ttctaagca aacgtgtca cggggggcg 
      atgcccaaa attatttct tgaagtaag agtagtgtt 
      gagtgtaat ataagaaat attacaaac atgatgtat 
      ttactttag ttaaaaaaa taaatatat gattttgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY