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Gene information
General information
TC003093 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1889047..1890200
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003093
    iBeetle screen iB_09739

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    Comment: Potentially one extra nucleus is present in few egg chambers in different ovarioles. 
    egg chamber mostlynot present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003093 RA
      MALVSYDYE SGSEEEEQE EPSTSSEVI INSTKVNNS 
      EANEDTTEL PEILSHLPQ TKTTTPTVN VTEDKLEDF 
      IPKYEPPPK ENKKTQKVK ITIPKLSEF EEEEEEEPQ 
      RKKTKTVSK SSGLLSMLP PVKGSPLST KSFVPNVLK 
      NKPPVTQKS KANSLVPHA VKKKAEMAK KAAIKKITK 
      GSSSDAESD DDIEVPETF DDEMWQKVC GRNTKPKPP 
      PVVNVEEVA PPVINIAPE PVKPYDGLD NAAFKELVG 
      KAKRPVNIK LVDINEEEI LPDKEIWMT KSLTDPDSA 
      PKPVVEEPV DPTRKKKHH ITYLAEQAK LNEQEFQSQ 
      WSASKYARN QTRAKYGF
    CDS sequence
    • >TC003093 RA
      atggctttg gtctcgtat gactatgaa agtggcagt 
      gaagaagaa gaacaagaa gaaccctca acttcttcg 
      gaagttata atcaatagc acaaaagtt aataacagt 
      gaagcaaat gaagacaca acggaacta ccagaaata 
      ttgtcacac cttcctcag actaaaacc acaacaccc 
      acagttaac gtgacggag gataaactg gaagatttt 
      atcccgaaa tatgaaccc cctcccaaa gaaaataag 
      aaaactcaa aaagtgaaa ataaccatt ccgaaacta 
      tcagagttc gaagaggaa gaagaggag gaaccacag 
      cgcaagaaa acgaaaacc gtttcgaaa agcagtggc 
      ttgttatcc atgctgccc ccagttaaa ggttcccca 
      ctctcaact aaaagtttc gtaccaaat gtcctaaaa 
      aacaaacct ccggtcacc caaaaatcg aaagccaac 
      agtttagtg ccacacgca gttaagaaa aaggcagaa 
      atggccaaa aaagccgca attaagaaa ataacaaaa 
      ggttcaagc agtgatgcc gaaagcgac gacgatatt 
      gaagtccct gagacgttc gacgacgaa atgtggcag 
      aaggtctgt ggacgaaac actaaaccc aagccgcct 
      ccagtcgtt aacgtggag gaagttgcg cctccggtg 
      ataaacatc gcacctgag ccggtgaaa ccgtacgat 
      ggtttggat aatgcggcg tttaaagag ctggtagga 
      aaggccaaa cggccggtt aatatcaag cttgtggac 
      attaatgag gaggagatt ttgcccgac aaggaaatc 
      tggatgact aagtcatta accgatcca gatagcgcc 
      cctaaaccg gtggttgag gaaccggtg gaccccaca 
      aggaagaag aaacaccac attacttat ctggcagaa 
      caggcgaaa ttgaacgaa caggagttc caaagtcaa 
      tggtctgct agtaaatat gcgcgaaat caaacacgg 
      gccaaatac ggtttttaa
    mRNA sequence
    • >TC003093 RA
      catcgattt ttgcagaaa ccatggctt tggtctcgt 
      atgactatg aaagtggca gtgaagaag aagaacaag 
      aagaaccct caacttctt cggaagtta taatcaata 
      gcacaaaag ttaataaca gtgaagcaa atgaagaca 
      caacggaac taccagaaa tattgtcac accttcctc 
      agactaaaa ccacaacac ccacagtta acgtgacgg 
      aggataaac tggaagatt ttatcccga aatatgaac 
      cccctccca aagaaaata agaaaactc aaaaagtga 
      aaataacca ttccgaaac tatcagagt tcgaagagg 
      aagaagagg aggaaccac agcgcaaga aaacgaaaa 
      ccgtttcga aaagcagtg gcttgttat ccatgctgc 
      ccccagtta aaggttccc cactctcaa ctaaaagtt 
      tcgtaccaa atgtcctaa aaaacaaac ctccggtca 
      cccaaaaat cgaaagcca acagtttag tgccacacg 
      cagttaaga aaaaggcag aaatggcca aaaaagccg 
      caattaaga aaataacaa aaggttcaa gcagtgatg 
      ccgaaagcg acgacgata ttgaagtcc ctgagacgt 
      tcgacgacg aaatgtggc agaaggtct gtggacgaa 
      acactaaac ccaagccgc ctccagtcg ttaacgtgg 
      aggaagttg cgcctccgg tgataaaca tcgcacctg 
      agccggtga aaccgtacg atggtttgg ataatgcgg 
      cgtttaaag agctggtag gaaaggcca aacggccgg 
      ttaatatca agcttgtgg acattaatg aggaggaga 
      ttttgcccg acaaggaaa tctggatga ctaagtcat 
      taaccgatc cagatagcg cccctaaac cggtggttg 
      aggaaccgg tggacccca caaggaaga agaaacacc 
      acattactt atctggcag aacaggcga aattgaacg 
      aacaggagt tccaaagtc aatggtctg ctagtaaat 
      atgcgcgaa atcaaacac gggccaaat acggttttt 
      aaaagtgtg tataatata ttaattaaa aaaataact 
      ggtggagaa cttgttatg caacgtttt gtctgttaa 
      ccaccactc atcaggcta aagaaaaaa ttaaacatc 
      ga
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