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Gene information
General information
TC003279 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 687330..691413
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003279
    iBeetle screen iB_09727

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003279 RA
      MFPFVIFTL VTLASADYW SERAKLVTS DSSQAIGSS 
      IILSPKEQE VNKILMNHK LKEYDDGFK NPRTFLPSI 
      HFFKSKSGI EQSPVFDFI RKLPKGASL HSHDTALVS 
      LDYLYNLTY RENLYGSVV KNRLQLHFF DKPPTSDWE 
      LVADLRSRN TSFDKFLKS QLSIIVDDP SSEYPSIND 
      VWNRFAETF YTVFGLVAY RPVFQDYFY QTLKELYED 
      NVMYLEFRG LLPQVYELN GTVYNHVGV VQLYLETLQ 
      RFKCDYPDF HGARFIFAP SRNVDNKTV EDYVTITKE 
      LRQLFPDFV AGFDLVGQE DLGKPLVDF IPQLLELAE 
      TDTRFFFHA GETDWGGTS TDLNVFDAV LLNTTRIGH 
      GFALVKHPK ILEEVKKRQ IAIEISPIS NQVLKLVDD 
      LRNHPGAFL VKSGFPVVI TCDDPTFWG AKALSYDWY 
      LAFMGFGGR EGDLRLLKQ LALNSLEFS AMAEDEKCD 
      ALAKWEEQW EVFLDGVVG GGKWGLSDW ISVNKV
    CDS sequence
    • >TC003279 RA
      atgtttccg ttcgttatt ttcacactt gtgacacta 
      gcaagtgcc gattattgg tcagaaaga gccaaactg 
      gtcacttcc gactcaagc caggccatt ggttcgtca 
      ataatccta tcacccaaa gaacaagaa gttaacaaa 
      attttaatg aaccacaaa ctcaaagaa tacgatgac 
      ggtttcaaa aacccacgt acttttctc ccatccata 
      catttcttc aagtcaaag agtggaatt gaacaatca 
      ccagtgttt gactttata cgaaaacta ccaaaaggg 
      gcatcgctt cattcacac gacactgcc ctagtctcg 
      cttgactat ctttacaat ctgacctat cgtgagaat 
      ttgtacggg agtgtggtc aaaaacaga ctccagttg 
      cattttttt gacaaacca ccaacaagt gactgggaa 
      ttggtggca gacctacgg tcacgcaac acctccttc 
      gataagttc ctcaagtca caattgtcc ataattgtg 
      gacgaccct tccagcgag tatccttcg attaacgat 
      gtttggaat agatttgcg gagacgttt tacaccgtt 
      tttggtcta gtggcttat agaccagtc ttccaggac 
      tacttctac caaacactt aaagaactg tacgaagac 
      aatgtcatg tacttggag tttcgtgga ctcctcccc 
      caggtctac gaactcaac ggtacagtg tacaaccac 
      gtgggagtc gtccaactg tacttggag acactccaa 
      cgcttcaaa tgcgattat cccgacttc cacggtgcg 
      cgatttatt ttcgcccca agtcgtaac gtggacaac 
      aaaaccgtc gaagactac gtcacaata accaaagaa 
      ctgagacag ttattcccc gatttcgtg gccggtttc 
      gacctggtg gggcaggag gacttgggc aaacccctc 
      gttgacttc atcccccag ctcctcgaa ctcgccgaa 
      accgacacg agattcttc ttccacgct ggtgagacc 
      gactggggc ggcacctcc accgacttg aacgtcttc 
      gatgccgtt ttgctcaac accaccagg attgggcac 
      gggtttgcg ctcgtgaaa caccccaaa attttggaa 
      gaggtgaaa aaacgccaa attgctata gagatcagt 
      ccgatttcg aatcaagtc ttgaagctt gttgatgat 
      ttgaggaat catccaggg gcgtttttg gtcaagtct 
      gggtttccg gttgtgata acgtgtgat gatccgact 
      ttttggggg gcgaaggct ttgagttat gattggtat 
      ttggcgttt atggggttt gggggaagg gagggggat 
      ttgaggttg ttgaagcag ttggcgttg aattcgttg 
      gagtttagt gcgatggcg gaggacgag aagtgtgat 
      gctttggcc aagtgggag gagcaatgg gaggtgttt 
      ttggatggg gttgtggga ggggggaag tgggggtta 
      agtgattgg attagtgta aacaaggtt tga
    mRNA sequence
    • >TC003279 RA
      ctgtttcaa taatatcta ttacaagta cagataaaa 
      tgaaccgat agtgataga ataaacgtt tcaaaaaca 
      acaagaaca aaaatttga taagtgata agataatcc 
      cttggttcc agcttcttt gaaaatgga aaaagtcgg 
      cgcccatga ctgtataac caaacttcc tcctcgtag 
      ctataaatc taacattgt ggtgattta tcgctacgt 
      aaggcaaac attgtgaca cgagcttga tagatcttt 
      agatattat ttcgatgag gcagagcgt tcacgaaaa 
      attgtcggg tttcacaca tctagataa agtactaca 
      ttttaagaa aaaaaaaaa aaaacaccc caagtgcac 
      cttcaaagt acggactga tggttgaaa tgtctccaa 
      aaattcaag tgaacacaa tccggctgt gcggccgtg 
      ataataata caatatctt tgatgtaga gttccaata 
      gattcatgt agtgtttat tttactaat tacgatttt 
      tagatatac gaacaataa tgtacattt tgaaggcag 
      acatcttgc ctagtgttc agcaaacag tatactatg 
      ctgttattt caaattaat tgaatatca gcaaaaacc 
      gtattgtaa ttaacaccg ggaagtaac agtgtcgta 
      gtgctacca acagatcaa gtgatttta atcatctgt 
      ctcttcaat aagtgacag ataagataa ttttcttgt 
      attcctcag taatctcag tacattagc agtgtctga 
      aataacaag tcatgtttc cgttcgtta ttttcacac 
      ttgtgacac tagcaagtg ccgattatt ggtcagaaa 
      gagccaaac tggtcactt ccgactcaa gccaggcca 
      ttggttcgt caataatcc tatcaccca aagaacaag 
      aagttaaca aaattttaa tgaaccaca aactcaaag 
      aatacgatg acggtttca aaaacccac gtacttttc 
      tcccatcca tacatttct tcaagtcaa agagtggaa 
      ttgaacaat caccagtgt ttgacttta tacgaaaac 
      taccaaaag gggcatcgc ttcattcac acgacactg 
      ccctagtct cgcttgact atctttaca atctgacct 
      atcgtgaga atttgtacg ggagtgtgg tcaaaaaca 
      gactccagt tgcattttt ttgacaaac caccaacaa 
      gtgactggg aattggtgg cagacctac ggtcacgca 
      acacctcct tcgataagt tcctcaagt cacaattgt 
      ccataattg tggacgacc cttccagcg agtatcctt 
      cgattaacg atgtttgga atagatttg cggagacgt 
      tttacaccg tttttggtc tagtggctt atagaccag 
      tcttccagg actacttct accaaacac ttaaagaac 
      tgtacgaag acaatgtca tgtacttgg agtttcgtg 
      gactcctcc cccaggtct acgaactca acggtacag 
      tgtacaacc acgtgggag tcgtccaac tgtacttgg 
      agacactcc aacgcttca aatgcgatt atcccgact 
      tccacggtg cgcgattta ttttcgccc caagtcgta 
      acgtggaca acaaaaccg tcgaagact acgtcacaa 
      taaccaaag aactgagac agttattcc ccgatttcg 
      tggccggtt tcgacctgg tggggcagg aggacttgg 
      gcaaacccc tcgttgact tcatccccc agctcctcg 
      aactcgccg aaaccgaca cgagattct tcttccacg 
      ctggtgaga ccgactggg gcggcacct ccaccgact 
      tgaacgtct tcgatgccg ttttgctca acaccacca 
      ggattgggc acgggtttg cgctcgtga aacacccca 
      aaattttgg aagaggtga aaaaacgcc aaattgcta 
      tagagatca gtccgattt cgaatcaag tcttgaagc 
      ttgttgatg atttgagga atcatccag gggcgtttt 
      tggtcaagt ctgggtttc cggttgtga taacgtgtg 
      atgatccga ctttttggg gggcgaagg ctttgagtt 
      atgattggt atttggcgt ttatggggt ttgggggaa 
      gggaggggg atttgaggt tgttgaagc agttggcgt 
      tgaattcgt tggagttta gtgcgatgg cggaggacg 
      agaagtgtg atgctttgg ccaagtggg aggagcaat 
      gggaggtgt ttttggatg gggttgtgg gagggggga 
      agtgggggt taagtgatt ggattagtg taaacaagg 
      tttgatttt gtgtgaata aaattattg attgaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY