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Gene information
General information
TC003184 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 577607..583268
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003184
    iBeetle screen iB_09724

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003184 RA
      MSDIKSFLH QFCQKTKKE QPQFEVRPT GPKHRQRFL 
      CEVRIDGYS YVGAGNSTN KKDAQSNAA RDFVSYLVR 
      EGIVNPADV PDFQDAPPG PNTVPMNRG PAPSVFRQG 
      MGPSSFGEA YRPLGRDHD DWHYMNRAQ EQKSVEDAE 
      DLDVNAQIH GNWTVENAK SKLHQFMQS NKIHADYKY 
      TQVGPDHSR TFVAEMSFY VKQLGRTIT GRDSGSNKQ 
      TASKSCALS IIRQLFHLG VIEAFSGTL KKEKTGSEM 
      KPFDVKISP DLQKQIKDC LDTLNITPV EVKPESESQ 
      PVSLLSNHI LDDFQASKP IAAGVVPWS PPQQNWNPW 
      TGCNIDEGP LANANLDEL SEHLAEETK QKLNHDVDL 
      KAIISERQG LPVYSMKGQ IMAAINDNP VIIIKGNTG 
      CGKTTQVCQ YILEDYIMS GQGAWCNIC ITQPRRISA 
      VSVSERVAN ERCEALGQS VGYSVRFES VLPRPYGSI 
      MFCTVGVLL KKLENGLRG VSHVIVDEI HERDVNSDF 
      IMVVLRDMI HTYPDLRVI LMSATIDTS LFSKYFNNC 
      PVVEIAGRT YPVQQYFLE DCVELTGFV PPTETRKRK 
      AGGGGGGGG DDDETTIGD DGDENMNKI IDPKFSTQT 
      RNAMARMSE REVSFELME ALLKYIKSQ EIPGAVLVF 
      LPGWNLIFA MMRHLQQHP VFGGAAYRV LPLHSQIPR 
      EDQRRVFEP VPPHVTKVI LATNIAETS ITINDVVFV 
      IDSCKAKIK LFTSHNNMT SYATVWASR TNLEQRKGR 
      AGRVRPGFC FHLCSKARF DKLDEHMTP EMFRTPLHE 
      LALSIKLLK LGSIGHFLS KAIEPPPLD AVIEAEVLL 
      REMKCLDSN DELTPLGRI IAKLPIEPR LGKMMVLGC 
      IFMCGGPLA TMAANSSTF PEIFTLDMG QRRLSHHQK 
      ALAGDRCSD HVAMLTAFE LWDQARAGG EEAEQRFCE 
      YKGISMPTM RVTWEAKHQ LQQILNSVG FPEETLSPI 
      PMDTVGPDP KLDVVMALM CYGLYPNVC YHKEKRKVL 
      TTESKAALI HKTSVNCSN FEQTFPYPF FVFGEKIRT 
      RAVSCKQMS MVTPIHLLL FGSRKIDWV DGVVRLDNW 
      INLNMDPEV AALIVALRP ALESLVVRA SQEPEQILE 
      MSPGDLKFV ATIKELCKL HAGSYQIER ARPQMQQSR 
      RPPRGHSLG FQGPPPKFF RGGSRGGFG DNRGGFRGG 
      FDGGHGFGG RGGFRGRGG FPRGRGFRG YSNY
    CDS sequence
    • >TC003184 RA
      atgtccgat ataaaatca ttcttgcac cagttctgc 
      cagaagacc aagaaagag cagccccag tttgaagtc 
      cggccaacc ggccccaaa caccgccag agattcctc 
      tgcgaagtc cgcatcgat ggctacagt tacgtgggg 
      gctggcaat tccaccaac aaaaaggac gcccaaagc 
      aacgccgcc cgcgatttc gtcagttac ctcgtccgg 
      gaaggcatt gttaacccc gccgacgtg cccgacttc 
      caggatgcg cctccaggt ccaaacacc gttccgatg 
      aatcggggg cccgcccct tcggtgttt cgacaggga 
      atggggccc tcgtcgttt ggggaggcc tacaggcct 
      ctggggcgc gatcacgac gattggcat tacatgaac 
      agggcgcag gaacagaag agtgtggag gatgcagag 
      gatttggat gtgaatgct cagattcat ggcaattgg 
      actgtggag aatgcaaag tcaaagctg catcagttt 
      atgcaaagc aataaaata cacgcggat tataagtat 
      acgcaagtg gggccagac cattccagg acttttgtg 
      gccgaaatg agcttctac gtcaagcaa ctcggacgt 
      acaataacc ggtcgtgat tccggttca aataagcaa 
      accgctagt aaaagttgc gctctttcc atcattcgc 
      caactgttc catctcggt gtaattgaa gcattttcc 
      ggaacactt aaaaaagag aaaaccggc agtgaaatg 
      aaacctttc gatgtaaaa atctcccca gatttacaa 
      aaacaaatc aaagattgc ctcgacact ttgaatatc 
      actccggtt gaggttaaa ccggaaagc gaaagccaa 
      ccggtttca cttttgagc aatcacatt ttggacgac 
      tttcaagcc tccaaaccg atagctgca ggagttgtc 
      ccatggtcg cctcctcaa caaaattgg aatccttgg 
      actgggtgc aacattgat gagggcccc cttgccaac 
      gccaacttg gacgagttg agcgaacat ttggccgaa 
      gaaaccaaa caaaaattg aatcatgac gttgatttg 
      aaagctatc attagtgag agacaaggt ttgccagtg 
      tattcaatg aaggggcaa attatggcc gcaattaac 
      gataatccc gttatcata attaaggga aataccgga 
      tgcggtaag actacacaa gtgtgtcag tacattctc 
      gaggattat attatgtcg ggtcaaggt gcctggtgc 
      aatatttgt ataacacag cctcggaga atttcggct 
      gtttccgtt tcggaacga gtggccaat gaacgttgc 
      gaagcattg ggacaatcg gttgggtat tcggttcgg 
      tttgaatcg gttcttccc cgaccttat ggttccatt 
      atgttttgt acggttggc gtgcttttg aagaagttg 
      gaaaatggg ctcagaggc gtcagtcat gtgattgtc 
      gacgaaata cacgaaagg gacgtcaat agtgacttt 
      attatggtg gttttacgc gacatgatc cacacctac 
      ccagacttg cgcgtaatt ctaatgtcg gcaacaatt 
      gacacgagt ttattttcg aaatatttc aacaattgt 
      ccagttgtt gaaatcgcc ggccgaact tatcccgtc 
      cagcagtac ttcctggag gactgtgtc gaactcacc 
      ggttttgtg ccacctaca gaaacacgc aaacgtaag 
      gctggagga ggcggtggt ggtggaggc gacgatgac 
      gaaacaacc attggagac gacggcgat gaaaacatg 
      aacaaaata atcgaccca aaattcagt actcaaacc 
      agaaatgca atggctcga atgagcgaa cgcgaagtg 
      agcttcgaa ttgatggaa gccttgctc aaatacatc 
      aaatcgcaa gaaattccg ggagctgtt ctagttttt 
      ctccccggt tggaacctg atttttgca atgatgcgc 
      cacttgcaa cagcatcca gtgtttgga ggggctgcc 
      tatagggtg ctgccgttg cattcgcag attccacga 
      gaggatcaa agacgtgtc tttgagccg gtgccccct 
      catgtcacc aaagttatt ctggcaaca aatattgcc 
      gaaacttcc atcacaatt aatgatgta gtgtttgtg 
      attgatagt tgcaaggcc aagattaaa ttgttcact 
      tcgcataat aatatgacg agttatgcg actgtttgg 
      gcctcaagg actaatctc gagcagagg aaaggacgt 
      gcagggaga gtcagaccg gggttttgt ttccatttg 
      tgttcgaag gcacgcttt gataaactg gacgaacat 
      atgacaccg gaaatgttc cgaacaccg ctgcatgaa 
      ttggcactg agtattaag ttgttaaag ttgggctcg 
      attgggcat tttttgagc aaggcgatc gagccaccg 
      ccactagat gccgtcatt gaagcagag gttttgtta 
      cgtgagatg aaatgtttg gacagtaac gatgagctg 
      acaccactt ggtcgcatc attgccaaa cttccgatt 
      gaaccacga ttggggaaa atgatggtt ctgggttgc 
      atttttatg tgtggtggt cccttggcc acaatggct 
      gctaattcg tccacattt cccgaaatt ttcacactt 
      gatatgggc cagagacgt ttaagccac caccagaag 
      gctctggct ggggatagg tgctctgat cacgtcgcc 
      atgttgaca gcttttgag ttgtgggat caagcacgt 
      gctggtggc gaagaagcc gagcaacgt ttctgcgaa 
      tacaaaggc ataagtatg ccgacaatg cgtgtcacg 
      tgggaggcc aaacaccaa ttgcaacaa attctcaat 
      tcggtgggt ttccccgaa gagacttta tcaccgatt 
      cctatggat acagttggc cctgatccg aaattggac 
      gttgtgatg gcattaatg tgttatggt ctttatcca 
      aatgtttgt tatcacaaa gagaaacga aaagttttg 
      acaaccgag agcaaggcc gccttgata cataaaaca 
      tcagtcaat tgcagtaac tttgaacaa acgttcccg 
      tatccattt tttgttttt ggggagaaa atacggaca 
      agggccgtt tcgtgtaaa caaatgagt atggtgacg 
      ccgattcat ttgttattg tttggttcg agaaaaatt 
      gattgggtt gatggtgtt gtgcgactt gataattgg 
      attaatttg aatatggac ccagaagtg gcggccctt 
      attgtggcc ctaaggccg gccctggaa agtttagtt 
      gttcgggcg tcgcaagaa ccggaacaa atactcgaa 
      atgtcacca ggagatctc aaatttgtt gcaacaatt 
      aaagagctg tgcaagttg cacgctggg agttaccaa 
      atcgagagg gctagaccc caaatgcaa caatcgagg 
      cgtccacca agggggcac tctttgggt tttcaagga 
      ccaccacca aagtttttc agagggggg tctcgaggg 
      ggctttgga gataatcgg ggtggtttt cgagggggt 
      tttgatggg ggccatgga tttggggga agagggggt 
      ttcagaggc aggggcgga tttccaaga ggtcgtggt 
      ttcagaggc tatagtaat tattaa
    mRNA sequence
    • >TC003184 RA
      tattctgtg gttaaatgt caaaacttt tccaaggtc 
      gtatttttt accggcatt ttcttcgat taaaaaata 
      cgaaatttc aataggatg tccgatata aaatcattc 
      ttgcaccag ttctgccag aagaccaag aaagagcag 
      ccccagttt gaagtccgg ccaaccggc cccaaacac 
      cgccagaga ttcctctgc gaagtccgc atcgatggc 
      tacagttac gtgggggct ggcaattcc accaacaaa 
      aaggacgcc caaagcaac gccgcccgc gatttcgtc 
      agttacctc gtccgggaa ggcattgtt aaccccgcc 
      gacgtgccc gacttccag gatgcgcct ccaggtcca 
      aacaccgtt ccgatgaat cgggggccc gccccttcg 
      gtgtttcga cagggaatg gggccctcg tcgtttggg 
      gaggcctac aggcctctg gggcgcgat cacgacgat 
      tggcattac atgaacagg gcgcaggaa cagaagagt 
      gtggaggat gcagaggat ttggatgtg aatgctcag 
      attcatggc aattggact gtggagaat gcaaagtca 
      aagctgcat cagtttatg caaagcaat aaaatacac 
      gcggattat aagtatacg caagtgggg ccagaccat 
      tccaggact tttgtggcc gaaatgagc ttctacgtc 
      aagcaactc ggacgtaca ataaccggt cgtgattcc 
      ggttcaaat aagcaaacc gctagtaaa agttgcgct 
      ctttccatc attcgccaa ctgttccat ctcggtgta 
      attgaagca ttttccgga acacttaaa aaagagaaa 
      accggcagt gaaatgaaa cctttcgat gtaaaaatc 
      tccccagat ttacaaaaa caaatcaaa gattgcctc 
      gacactttg aatatcact ccggttgag gttaaaccg 
      gaaagcgaa agccaaccg gtttcactt ttgagcaat 
      cacattttg gacgacttt caagcctcc aaaccgata 
      gctgcagga gttgtccca tggtcgcct cctcaacaa 
      aattggaat ccttggact gggtgcaac attgatgag 
      ggccccctt gccaacgcc aacttggac gagttgagc 
      gaacatttg gccgaagaa accaaacaa aaattgaat 
      catgacgtt gatttgaaa gctatcatt agtgagaga 
      caaggtttg ccagtgtat tcaatgaag gggcaaatt 
      atggccgca attaacgat aatcccgtt atcataatt 
      aagggaaat accggatgc ggtaagact acacaagtg 
      tgtcagtac attctcgag gattatatt atgtcgggt 
      caaggtgcc tggtgcaat atttgtata acacagcct 
      cggagaatt tcggctgtt tccgtttcg gaacgagtg 
      gccaatgaa cgttgcgaa gcattggga caatcggtt 
      gggtattcg gttcggttt gaatcggtt cttccccga 
      ccttatggt tccattatg ttttgtacg gttggcgtg 
      cttttgaag aagttggaa aatgggctc agaggcgtc 
      agtcatgtg attgtcgac gaaatacac gaaagggac 
      gtcaatagt gactttatt atggtggtt ttacgcgac 
      atgatccac acctaccca gacttgcgc gtaattcta 
      atgtcggca acaattgac acgagttta ttttcgaaa 
      tatttcaac aattgtcca gttgttgaa atcgccggc 
      cgaacttat cccgtccag cagtacttc ctggaggac 
      tgtgtcgaa ctcaccggt tttgtgcca cctacagaa 
      acacgcaaa cgtaaggct ggaggaggc ggtggtggt 
      ggaggcgac gatgacgaa acaaccatt ggagacgac 
      ggcgatgaa aacatgaac aaaataatc gacccaaaa 
      ttcagtact caaaccaga aatgcaatg gctcgaatg 
      agcgaacgc gaagtgagc ttcgaattg atggaagcc 
      ttgctcaaa tacatcaaa tcgcaagaa attccggga 
      gctgttcta gtttttctc cccggttgg aacctgatt 
      tttgcaatg atgcgccac ttgcaacag catccagtg 
      tttggaggg gctgcctat agggtgctg ccgttgcat 
      tcgcagatt ccacgagag gatcaaaga cgtgtcttt 
      gagccggtg ccccctcat gtcaccaaa gttattctg 
      gcaacaaat attgccgaa acttccatc acaattaat 
      gatgtagtg tttgtgatt gatagttgc aaggccaag 
      attaaattg ttcacttcg cataataat atgacgagt 
      tatgcgact gtttgggcc tcaaggact aatctcgag 
      cagaggaaa ggacgtgca gggagagtc agaccgggg 
      ttttgtttc catttgtgt tcgaaggca cgctttgat 
      aaactggac gaacatatg acaccggaa atgttccga 
      acaccgctg catgaattg gcactgagt attaagttg 
      ttaaagttg ggctcgatt gggcatttt ttgagcaag 
      gcgatcgag ccaccgcca ctagatgcc gtcattgaa 
      gcagaggtt ttgttacgt gagatgaaa tgtttggac 
      agtaacgat gagctgaca ccacttggt cgcatcatt 
      gccaaactt ccgattgaa ccacgattg gggaaaatg 
      atggttctg ggttgcatt tttatgtgt ggtggtccc 
      ttggccaca atggctgct aattcgtcc acatttccc 
      gaaattttc acacttgat atgggccag agacgttta 
      agccaccac cagaaggct ctggctggg gataggtgc 
      tctgatcac gtcgccatg ttgacagct tttgagttg 
      tgggatcaa gcacgtgct ggtggcgaa gaagccgag 
      caacgtttc tgcgaatac aaaggcata agtatgccg 
      acaatgcgt gtcacgtgg gaggccaaa caccaattg 
      caacaaatt ctcaattcg gtgggtttc cccgaagag 
      actttatca ccgattcct atggataca gttggccct 
      gatccgaaa ttggacgtt gtgatggca ttaatgtgt 
      tatggtctt tatccaaat gtttgttat cacaaagag 
      aaacgaaaa gttttgaca accgagagc aaggccgcc 
      ttgatacat aaaacatca gtcaattgc agtaacttt 
      gaacaaacg ttcccgtat ccatttttt gtttttggg 
      gagaaaata cggacaagg gccgtttcg tgtaaacaa 
      atgagtatg gtgacgccg attcatttg ttattgttt 
      ggttcgaga aaaattgat tgggttgat ggtgttgtg 
      cgacttgat aattggatt aatttgaat atggaccca 
      gaagtggcg gcccttatt gtggcccta aggccggcc 
      ctggaaagt ttagttgtt cgggcgtcg caagaaccg 
      gaacaaata ctcgaaatg tcaccagga gatctcaaa 
      tttgttgca acaattaaa gagctgtgc aagttgcac 
      gctgggagt taccaaatc gagagggct agaccccaa 
      atgcaacaa tcgaggcgt ccaccaagg gggcactct 
      ttgggtttt caaggacca ccaccaaag tttttcaga 
      ggggggtct cgagggggc tttggagat aatcggggt 
      ggttttcga gggggtttt gatgggggc catggattt 
      gggggaaga gggggtttc agaggcagg ggcggattt 
      ccaagaggt cgtggtttc agaggctat agtaattat 
      taattgtat cggtagaca taaatgtgt ttgatcatc 
      tcggtattc aaaaattaa aaattttaa caaaataaa 
      tattagttt tttcttctc gaacttgag gccttggct 
      cactagatt cactacaaa gaacaaaac aatatatat 
      tttttgttg agtttgtta ataaaattt aaatttgaa 
      g
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