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Gene information
General information
TC032292
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003263
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 521800..524701
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032292
    iBeetle screen iB_09723

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032292 RA
      MDFSGIWNQ LLINPTTVC VQPNPLIEG 
      IQVITPYVT SSDTNSSIN SESSVPIQE 
      NETEKNEEI NSPEDEETN EKNDQKKTI 
      QRVRYSKEE YRCMQCPYT CTTEKAFLH 
      HLRWCEDKS PKRLLSCPV CGKDRKSEE 
      ILSIHMMKH RQDKYFCCD FCKFKTIQL 
      KKLIQHRRM HTGEKPHLC PFCSYRSSR 
      RDNLRSHVR RVHKKDDLP CDTFTPRSF 
      VLSPRKTVN ECN
    CDS sequence
    • >TC032292 RA
      atggatttt tcgggaatt tggaaccaa cttttaata 
      aatccaacc acggtgtgc gtccaaccg aatccatta 
      attgaagga attcaagta attacaccc tacgtcact 
      tcaagcgac acaaattcg tcgattaat tcagagagc 
      agtgtgccg attcaagag aacgaaacc gaaaaaaat 
      gaagaaatt aacagtcca gaagacgaa gaaactaac 
      gaaaaaaat gaccaaaag aaaacaatt caaagagtt 
      agatattcg aaagaggaa tatcggtgc atgcaatgc 
      ccttacact tgcaccacc gaaaaggcg tttttgcac 
      cacttgaga tggtgcgaa gataaatcc cctaaaaga 
      ctcctctcg tgtccagtg tgtggcaaa gatcgaaaa 
      agcgaggaa atcctttcg attcatatg atgaagcac 
      agacaggac aagtatttt tgttgtgat ttttgtaaa 
      tttaaaacc attcaattg aaaaagctc attcaacat 
      cggaggatg catacaggg gaaaaaccc catttgtgt 
      cccttttgc agttaccga tcgtcacgc agagacaat 
      ttaaggtct catgtgaga agagtccat aaaaaagac 
      gatttgccg tgtgatacc tttacgcca agaagtttt 
      gttctgtct cccaggaaa acagttaat gaatgtaat 
      taa
    mRNA sequence
    • >TC032292 RA
      aacagactg aaaaataac cagaaaaat acgacatta 
      aaaattata taagtcact tttgaacga aaaattgca 
      ttaaaataa gccgcacaa cctatacct aattaaaaa 
      tctgatcat tttgagcga aaaatttac taatttatg 
      taaaaagac taaaaaatg taatggggg tagttgaaa 
      caaaaaata ttatttctg gaaaattac cagtgtatt 
      agaaactgg tccaaacat tgtcgtact ggtggaact 
      tgtattttt tactttaag gaaccttaa tctcccata 
      tgtgatttt ttcagttac tagtgtcaa aataaaatt 
      ttaaaattg tctagaggc tgtctcagc aaagatttt 
      ctctggccg gaaaagtat ttgccgaga cagcttgta 
      cgtaaatag ttcgaatga atgacttat ataaaagat 
      aaaaaaagt tgttttgtt cccatttcg gatggttcg 
      taccaacta aataattct aaatttcct taatttgat 
      aattataag gcaaaaaga acgtattgc cgtcctaaa 
      tacgaatca aactataaa ccacactaa ttggtagcg 
      ccggtacaa gccattcgt ttttgtttc gtgtttaaa 
      taattgtaa ataacttac tggttacct attatacta 
      aaaaaatac ctatatcat attatgcaa tttgcttga 
      aatgtaaaa attttatta gaatggtta gtaatccac 
      ttgcatggt ttttaggaa caaaattct tctcaacaa 
      catattcta gaaatcatg caagtcgtt tgatttaaa 
      aagcacaaa acgtgttgt gcaaacgtt cacaattca 
      aaaaagtga atttgtatt taaaaaact actattaga 
      tagttttgc taatccata tcataatta ctacattgt 
      tttattata aatataaat gagatttca cacaaatat 
      agaagctga aagcttgac gtagataag acttcgatt 
      cgattttca ttcgatttt tgtgccgcc atgggcaca 
      agtcttggt taaaccaac gagctcaca aaaattatg 
      ttactcgag taaagtttt ctcgatttt gttacagtc 
      ggaacagga aagctaaaa tttgtggcc tcgttgccc 
      atttgatgt ttttgacct taatttagt cgattcaac 
      ttgaataaa tgtcttgtg ttctatgtt gcattgttt 
      tatgtacca agttaactt gtaatacat tataaatac 
      acattatcg attcaatta caatattac aagtttcac 
      aaatccgtg ttttattat tccaatttg gcgccctgc 
      tgtttacca gttttttat ttattattt tgaccatcc 
      ctcaaaccc gattcacgc acttggaaa caacatctt 
      gttgcgaga tgaaacaga cacacctgt cctaaattt 
      cctccctgt gatctcggc ttttccaat aatgttgta 
      tataatata ttattcgaa atgtacaaa aacgcacaa 
      ataggaaac caacaaatg tgatggatt tttcgggaa 
      tttggaacc aacttttaa taaatccaa ccacggtgt 
      gcgtccaac cgaatccat taattgaag gaattcaag 
      taattacac cctacgtca cttcaagcg acacaaatt 
      cgtcgatta attcagaga gcagtgtgc cgattcaag 
      agaacgaaa ccgaaaaaa atgaagaaa ttaacagtc 
      cagaagacg aagaaacta acgaaaaaa atgaccaaa 
      agaaaacaa ttcaaagag ttagatatt cgaaagagg 
      aatatcggt gcatgcaat gcccttaca cttgcacca 
      ccgaaaagg cgtttttgc accacttga gatggtgcg 
      aagataaat cccctaaaa gactcctct cgtgtccag 
      tgtgtggca aagatcgaa aaagcgagg aaatccttt 
      cgattcata tgatgaagc acagacagg acaagtatt 
      tttgttgtg atttttgta aatttaaaa ccattcaat 
      tgaaaaagc tcattcaac atcggagga tgcatacag 
      gggaaaaac cccatttgt gtccctttt gcagttacc 
      gatcgtcac gcagagaca atttaaggt ctcatgtga 
      gaagagtcc ataaaaaag acgatttgc cgtgtgata 
      cctttacgc caagaagtt ttgttctgt ctcccagga 
      aaacagtta atgaatgta attaaatta agtgttgtt 
      gttttacga tatgtaaat ttttataat aattttact 
      tgtaatgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY