Status
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Gene information
General information
TC003222 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 61686..65753
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003222
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09718

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003222 RA
      MKKQFFRVK QLADQTFLR ADKSEVLNN EELQVADQK 
      IEYLRTALS AIIKRIPHS GQNNDIDKR IKKSIEHHL 
      ASVFFEQST RKNEQGDLQ ELPNDLFCA TLKKCALVE 
      QDLAREYAD HEKQVEDLV SAPIQALLD SDFHNILKQ 
      KRNLSKYIL DKDSASNRY HATKKEALR DDMEEADYK 
      VEQSRDALA YEMFSLLAK ENDLAGYML QILKCQRAY 
      HDSALKHLE SVIPELEKQ IGDSSVKRV YGISLQDHL 
      RVTNKKIAL PLEICISIL QKHGLHEEG LFRIAGSMS 
      RVKRLKSSI DSGCFSPKL IPEYQDMHV LASALKMYL 
      RELPDPLLT SKLYNEWLQ SMQKPESER LDIVKGLIA 
      SLPRENRDN LAFLIQFLS ELSRHPQNK MSSSNIAIV 
      VAPNLLWDK EETMNMGNC AASSMLVEL FIKEVHTLF 
      PDDVSKFVS FSWTDDEGG FTRSTIDLN ASTENISES 
      PKPNSRKKK TPAPGPPPN NFARTDQDL VQNEKNSMS 
      SSYPSGSST LNRPQKPRD KPKTSIGVN TDDRRMSLS 
      QEETHKSEV IKSAPLVNL DSSKTVAHK VITEPATLV 
      QVSKAKPVQ PVVAQNACD NKNFKTTVT HSMDGGVCQ 
      KPIAAPRSI DAGVKRSSS IKIDLKQPE MTEVTMRRP 
      EPAAKPEVP ARPASLSKR PSMDLDPTL HRTQCSVYS 
      VANKQQPSY VNVQNRNEK FQPGHDNKI AEKERFLGH 
      QPALKLDDI NSNSKTGLP PKAIKCVEK SEASKSNED 
      LGDLGEKLN GNQKTSHVR TRSDGNLIE LSDGCLAQT 
      PPSPRSLNK PTQPPPPPP IVSVVKKEP DSTDL
    CDS sequence
    • >TC003222 RA
      atgaagaag caatttttc cgagtcaaa caactcgcc 
      gaccagact ttcctcagg gccgacaaa agcgaggtt 
      ttaaataat gaagaactt caagtggct gatcagaaa 
      atcgaatac ttacgaacg gctctatct gcaatcatc 
      aagcggata ccacattcg ggccaaaat aatgacatc 
      gacaaacga attaaaaag agcattgaa catcacctg 
      gcctctgtg ttttttgag caaagtact cgaaaaaat 
      gaacaaggg gaccttcag gaactacca aatgatttg 
      ttttgtgcc acgcttaaa aaatgtgca ttggttgag 
      caggatttg gcgagggaa tatgctgat catgaaaaa 
      caagttgag gatttagtt agtgctcca atccaggcg 
      ttgcttgat tccgatttt cataatatc ctaaaacag 
      aaacgcaat ctttcgaaa tatattttg gataaagat 
      tcggcaagt aataggtac catgctacg aaaaaagag 
      gctttgcgg gatgatatg gaagaagcc gactataaa 
      gtagaacaa agtagggac gccttagct tatgaaatg 
      tttagttta ttagcgaaa gaaaatgac ttggccggc 
      tacatgttg caaatactg aaatgtcaa agagcgtat 
      catgatagt gccttaaag catctggaa agtgtgatt 
      cctgagttg gaaaagcaa ataggtgac agttcagtg 
      aaacgcgtt tatggcatt tcgcttcaa gaccacctc 
      cgagtcaca aataaaaaa atagcgctt cctttagaa 
      atttgtatt agcattctt caaaaacat ggtcttcac 
      gaagaaggt ctttttcgt attgctggc agtatgtca 
      cgcgtcaaa cgcttgaaa tcatcaata gattcaggc 
      tgtttttcc ccgaaatta attccagag tatcaggac 
      atgcatgtg ttggccagt gcccttaaa atgtatctc 
      cgtgaatta cccgatcca cttctaacg tcaaaactt 
      tacaatgag tggttgcaa tcgatgcaa aaacccgaa 
      agcgaaaga ctggacatt gttaaaggt ctaatcgcg 
      agtttgcct cgtgaaaat cgtgataat ttagcgttt 
      ttgattcaa tttttatcg gaattgtct cgtcatccg 
      caaaacaag atgagttcg tccaatatt gcaattgtt 
      gttgctccg aatttattg tgggataaa gaggagacc 
      atgaatatg ggaaattgt gcagctagt agcatgctc 
      gttgagctt tttatcaaa gaagttcac accttgttt 
      cctgatgat gttagtaag tttgtgagt ttttcatgg 
      actgatgat gaaggggga ttcacccga tcgacgatc 
      gacttgaac gcaagcacc gaaaatatc agtgaaagt 
      cctaaaccc aactcgagg aaaaagaaa actccagca 
      cctggacct ccccccaat aactttgcc cgaactgac 
      caagatctc gtccaaaac gagaaaaat tcaatgtca 
      tcgtcatat ccttcagga agttccacc ttaaaccgt 
      cctcaaaaa ccccgcgat aaaccgaag acttcaatt 
      ggtgttaac accgacgac cgaagaatg agtttatct 
      caagaggaa acgcacaaa tcggaagtg atcaaatca 
      gcgcctcta gtcaattta gatagtagc aaaacggtc 
      gctcataag gttataacc gaaccggcg actctcgtt 
      caagtgtcg aaagccaaa cctgtacag ccagtcgta 
      gcacaaaac gcatgtgat aataaaaac tttaaaaca 
      actgtcaca cattcaatg gacggtggc gtgtgtcag 
      aaaccgatc gcagcgcca cgctcgatt gatgccgga 
      gtcaagcga agttcctca atcaagatc gatcttaag 
      cagccggaa atgaccgaa gttacgatg cgaaggccc 
      gaacccgcc gccaaacca gaggtgcct gcaaggccg 
      gcctcactc tcgaaacgg ccgagtatg gacctggat 
      ccaaccctg caccgcacc cagtgctca gtctacagt 
      gtagccaac aaacaacaa cccagctac gtcaacgtc 
      cagaaccgt aatgagaaa ttccagccg ggtcacgac 
      aacaaaatc gccgaaaaa gagcgtttt ctaggccac 
      cagccagcg ctcaaactc gacgatatc aattcaaac 
      agtaaaaca gggctgcca cctaaagca ataaaatgt 
      gtggagaaa tcggaagcg agcaaatca aatgaggat 
      ttgggcgat ttgggagaa aaactgaac ggtaatcag 
      aaaactagt catgtgcga accagatcg gacggaaat 
      ttgattgaa ctaagtgat ggttgtttg gctcagacg 
      ccgccgtca ccacgcagt ttgaataaa ccgacacag 
      ccgccaccg ccgcccccg attgttagt gttgtcaag 
      aaagagcct gacagtact gatttgtag
    mRNA sequence
    • >TC003222 RA
      ccagtaata gaaagtgat gtggttgtg ttggtcttt 
      ttttgtcgc ataaccaat gatgattta ttgatattt 
      tagcatttg tgttaattt gtgacgttt gtacaccgc 
      tacaataat gccaaaaag tgtcgttat tgacgcaat 
      cgaatgaaa agtcatact tttgtttac gtgaaatgg 
      aaagtgata tttgccttc tctatcaca atatgatct 
      catcacgta aaagttaat aataattcc gattggtgc 
      attatcggc tttttaaat accgatttt taaataccg 
      aatttattt ggaatttta taatgaaga agcaatttt 
      tccgagtca aacaactcg ccgaccaga ctttcctca 
      gggccgaca aaagcgagg ttttaaata atgaagaac 
      ttcaagtgg ctgatcaga aaatcgaat acttacgaa 
      cggctctat ctgcaatca tcaagcgga taccacatt 
      cgggccaaa ataatgaca tcgacaaac gaattaaaa 
      agagcattg aacatcacc tggcctctg tgttttttg 
      agcaaagta ctcgaaaaa atgaacaag gggaccttc 
      aggaactac caaatgatt tgttttgtg ccacgctta 
      aaaaatgtg cattggttg agcaggatt tggcgaggg 
      aatatgctg atcatgaaa aacaagttg aggatttag 
      ttagtgctc caatccagg cgttgcttg attccgatt 
      ttcataata tcctaaaac agaaacgca atctttcga 
      aatatattt tggataaag attcggcaa gtaataggt 
      accatgcta cgaaaaaag aggctttgc gggatgata 
      tggaagaag ccgactata aagtagaac aaagtaggg 
      acgccttag cttatgaaa tgtttagtt tattagcga 
      aagaaaatg acttggccg gctacatgt tgcaaatac 
      tgaaatgtc aaagagcgt atcatgata gtgccttaa 
      agcatctgg aaagtgtga ttcctgagt tggaaaagc 
      aaataggtg acagttcag tgaaacgcg tttatggca 
      tttcgcttc aagaccacc tccgagtca caaataaaa 
      aaatagcgc ttcctttag aaatttgta ttagcattc 
      ttcaaaaac atggtcttc acgaagaag gtctttttc 
      gtattgctg gcagtatgt cacgcgtca aacgcttga 
      aatcatcaa tagattcag gctgttttt ccccgaaat 
      taattccag agtatcagg acatgcatg tgttggcca 
      gtgccctta aaatgtatc tccgtgaat tacccgatc 
      cacttctaa cgtcaaaac tttacaatg agtggttgc 
      aatcgatgc aaaaacccg aaagcgaaa gactggaca 
      ttgttaaag gtctaatcg cgagtttgc ctcgtgaaa 
      atcgtgata atttagcgt ttttgattc aatttttat 
      cggaattgt ctcgtcatc cgcaaaaca agatgagtt 
      cgtccaata ttgcaattg ttgttgctc cgaatttat 
      tgtgggata aagaggaga ccatgaata tgggaaatt 
      gtgcagcta gtagcatgc tcgttgagc tttttatca 
      aagaagttc acaccttgt ttcctgatg atgttagta 
      agtttgtga gtttttcat ggactgatg atgaagggg 
      gattcaccc gatcgacga tcgacttga acgcaagca 
      ccgaaaata tcagtgaaa gtcctaaac ccaactcga 
      ggaaaaaga aaactccag cacctggac ctcccccca 
      ataactttg cccgaactg accaagatc tcgtccaaa 
      acgagaaaa attcaatgt catcgtcat atccttcag 
      gaagttcca ccttaaacc gtcctcaaa aaccccgcg 
      ataaaccga agacttcaa ttggtgtta acaccgacg 
      accgaagaa tgagtttat ctcaagagg aaacgcaca 
      aatcggaag tgatcaaat cagcgcctc tagtcaatt 
      tagatagta gcaaaacgg tcgctcata aggttataa 
      ccgaaccgg cgactctcg ttcaagtgt cgaaagcca 
      aacctgtac agccagtcg tagcacaaa acgcatgtg 
      ataataaaa actttaaaa caactgtca cacattcaa 
      tggacggtg gcgtgtgtc agaaaccga tcgcagcgc 
      cacgctcga ttgatgccg gagtcaagc gaagttcct 
      caatcaaga tcgatctta agcagccgg aaatgaccg 
      aagttacga tgcgaaggc ccgaacccg ccgccaaac 
      cagaggtgc ctgcaaggc cggcctcac tctcgaaac 
      ggccgagta tggacctgg atccaaccc tgcaccgca 
      cccagtgct cagtctaca gtgtagcca acaaacaac 
      aacccagct acgtcaacg tccagaacc gtaatgaga 
      aattccagc cgggtcacg acaacaaaa tcgccgaaa 
      aagagcgtt ttctaggcc accagccag cgctcaaac 
      tcgacgata tcaattcaa acagtaaaa cagggctgc 
      cacctaaag caataaaat gtgtggaga aatcggaag 
      cgagcaaat caaatgagg atttgggcg atttgggag 
      aaaaactga acggtaatc agaaaacta gtcatgtgc 
      gaaccagat cggacggaa atttgattg aactaagtg 
      atggttgtt tggctcaga cgccgccgt caccacgca 
      gtttgaata aaccgacac agccgccac cgccgcccc 
      cgattgtta gtgttgtca agaaagagc ctgacagta 
      ctgatttgt agttgaaag agtgatttt tttgcagag 
      tttgtaagt ctattgaac aaatagttg ctatttgac 
      tgaagttaa aattttaaa ttttaatgt tgatgttaa 
      agttatgaa acggatttt atttattcg gatggctac 
      tggttaata aggcacacg aaataccaa tttattata 
      atgctttaa caaaactga ttgcagtat aatttttag 
      attttagga attttgata ttctttcat tttatcaac 
      aaaagtgct gtacgagac acaatcaat aaaaagttc 
      gttatttat atttcattg aacatcaga ttgcccact 
      ttccgttta tgtgaacca aatccaaac acttccttc 
      taaaaatac cctaaagac ccaatacaa tagtttcag 
      tcgcgaatt gcacagcaa aatggaatg ttttaccaa 
      caagtacgc gttcaaaat ttacgattc ggtcgatta 
      tgacggaat tacacttcc ccgaaaccg ttcaattat 
      ccaacccat ttatatttt acaccagga ttactttta 
      ccagttcta ttacggtaa gtggaaaga aactattcg 
      attctca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY