Status
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Gene information
General information
TC001355 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15011261..15015454
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001355
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09710

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001355 RA
      MWRFCALCV CLLALGVIA QYEWQARDA YDEVRRAMD 
      RVNKYNCEI QHVGDLYLP ETSVSHIPD IKDVNINPV 
      FPNRTQLLH IHNMALSRA FFWSYILQS RFIRPAIND 
      TYDPGMMYY FLSTVADVS TNNHVNASA IYFAPNMSY 
      SSSYRGFFN KTFPRFAPR TFRADDFND PIHLERIST 
      LNTFTVYDL GAIKPNTSD DYTSDFYRI NEWYKKWLP 
      DRVFDRHDT KTTYQIYIR YANNTNETF TFHGPPGAD 
      ESPGPVQWT KPYFDCGRS NRWLVAAVV PIADIYPRH 
      TGFRHIEYP TYTAVSVVE MDYERIDIN QCPPGLGNK 
      EGLNMFADT ARCKKETTE CEPIHGWGF RRGGYQCRC 
      KPGYRLPMQ VRRPFLGEI VERATAEQY YNGFDCLKI 
      GWVQKLPVQ WEKASSSVR NHYLDKYRE YRYPVSGPE 
      ALHQPKINI HKSLDFILG MNKDNCKNY NKDDLILYG 
      GVEYGAYEF FQNEAKMAL RLANFISAF LQVNDPTEI 
      YSGKRVPDR PLTEDEMIG ETLALVMGN SRIWSAGTY 
      WERNKFTNR TLFAPYAYK TQLNTRKFA VEDLARLNK 
      SDEVYLNKP WFLKLKQRW LTNFDSLEK YYMKIKIRF 
      NETGETTRK YERYPNFYR GAQLKHGLW TEPYYDCDG 
      KKPMWKIKY AAPFFGWDS LKVKLEFKG VVTVSMDLL 
      KLDINQCPD RYYVPNAFK NTHKCDLRN SYCVPILGR 
      GFETGGYKC ECKQGYEYP FEDLITYYD GQVLEAEFS 
      NLVDNNETR FDMFKCRLA GASSVQVSI VTVLLLIFV 
      SFGLYGRS
    CDS sequence
    • >TC001355 RA
      atgtggcgt ttttgtgct ttatgtgtg tgtttactc 
      gcactgggt gtgatcgcc cagtacgaa tggcaagca 
      agggatgcc tatgatgag gtgcgtcga gccatggac 
      cgagtgaat aaatacaat tgtgagatt caacatgtt 
      ggagatttg tatcttccg gaaacgtcc gtatcccac 
      attcccgat attaaggat gtcaacatt aatcctgtt 
      tttcccaac cggactcaa ctgctccac attcacaac 
      atggcactg agtcgtgcc ttcttctgg agttacatc 
      ctccagagt cgattcatt cgaccggct attaacgac 
      acctacgac cccggaatg atgtactat ttcctctcc 
      accgtggcc gacgtctcc accaataac cacgtcaat 
      gctagtgct atttatttc gcccccaac atgtcgtac 
      tcatcgtca taccgtggc ttcttcaac aagacgttc 
      cctaggttt gcaccaagg acattccgc gcggatgat 
      ttcaacgac cctatccat ttagaacgt atttccact 
      ttgaatacg ttcactgtg tatgattta ggggcaatt 
      aaacccaat actagcgat gactatact tcagatttt 
      taccgaatt aatgaatgg tacaaaaag tggttgcct 
      gatcgtgtt tttgataga catgacacc aaaacgact 
      tatcagatt tatatcaga tatgcaaac aatacgaat 
      gaaacgttt acgtttcat ggaccacct ggtgctgat 
      gaaagtcca gggcctgtt caatggaca aaaccgtat 
      tttgattgc gggagatct aatagatgg ttggtggcg 
      gctgttgtg ccaattgcc gatatttat ccaaggcat 
      acagggttt aggcatata gaatatccc acttacact 
      gctgtttct gtggttgaa atggactac gaacgtatc 
      gacatcaac caatgtcca cccggtcta gggaacaaa 
      gaaggtctc aacatgttt gctgacaca gcccgatgt 
      aaaaaagaa acaaccgaa tgcgaaccg attcacggt 
      tggggcttc cggagaggt ggttatcag tgtcgttgc 
      aaacccggt tatcgtctc cctatgcaa gtccgacgt 
      ccctttttg ggagaaata gtcgaacgt gcaacggca 
      gaacaatac tacaacggt tttgactgt cttaaaatc 
      ggatgggtt caaaaatta ccggttcag tgggaaaaa 
      gcaagttcg tcggtccgt aaccactac ctcgataaa 
      taccgggag tatcgttat cccgtctca ggacctgag 
      gctttacac caacccaaa atcaacatc cacaaatca 
      ctcgatttc attttgggc atgaataag gataattgc 
      aagaattac aacaaggac gatttaatt ttatacggc 
      ggtgtcgaa tacggcgct tatgagttt ttccaaaac 
      gaagccaaa atggcctta cgacttgca aatttcatt 
      agtgccttt ttacaagtc aatgaccct actgaaatt 
      tattcgggg aaacgagtc cctgatcga ccgctaaca 
      gaagacgaa atgattggc gaaactttg gcccttgtt 
      atgggcaat agccgaatt tggtcggct ggtacatat 
      tgggaacga aacaaattc acaaaccgc acacttttc 
      gcaccatac gcctacaaa acgcaacta aacacgcga 
      aaattcgca gttgaggac ttggcccga ctcaacaaa 
      tctgacgaa gtctatttg aataaaccc tggtttttg 
      aaattgaaa caacgctgg ttgaccaac tttgacagt 
      ctcgaaaaa tattacatg aaaataaaa attcgcttt 
      aacgaaacg ggcgaaacg acaagaaag tacgaacgc 
      tatccaaac ttctacaga ggggcccaa ttaaagcac 
      ggcttatgg accgaaccg tattacgat tgcgatggt 
      aaaaaaccc atgtggaag attaaatac gcggcaccg 
      tttttcggt tgggacagt cttaaagtc aaactcgaa 
      ttcaagggc gtggtcacc gtatcaatg gatttgtta 
      aaacttgat ataaaccaa tgtcccgac agatattac 
      gtcccaaac gccttcaaa aacacacac aagtgcgat 
      ttgcgaaac tcatattgt gttccgatt ttgggccgt 
      ggattcgaa acaggcggc tacaagtgt gagtgtaag 
      caaggttac gaatatccg tttgaagat ctcatcact 
      tattacgac ggtcaagtg ttggaagcc gaatttagt 
      aatttagtt gataataat gagacacgt tttgacatg 
      ttcaagtgt cgcttagct ggcgctagc agtgttcaa 
      gttagtatc gttacagtt ttgttactg atatttgtt 
      agtttcggt ttgtatggc agaagttga
    mRNA sequence
    • >TC001355 RA
      ctaacgtaa cctaagatt taaaaccgt caaaacaaa 
      gcaaaacca cccctaaaa tcgccaccc cccgaaaga 
      caatgacgt gctaatcat acaagtgtt gttgtttag 
      acgaaaaaa tgtggcgtt tttgtgctt tatgtgtgt 
      gtttactcg cactgggtg tgatcgccc agtacgaat 
      ggcaagcaa gggatgcct atgatgagg tgcgtcgag 
      ccatggacc gagtgaata aatacaatt gtgagattc 
      aacatgttg gagatttgt atcttccgg aaacgtccg 
      tatcccaca ttcccgata ttaaggatg tcaacatta 
      atcctgttt ttcccaacc ggactcaac tgctccaca 
      ttcacaaca tggcactga gtcgtgcct tcttctgga 
      gttacatcc tccagagtc gattcattc gaccggcta 
      ttaacgaca cctacgacc ccggaatga tgtactatt 
      tcctctcca ccgtggccg acgtctcca ccaataacc 
      acgtcaatg ctagtgcta tttatttcg cccccaaca 
      tgtcgtact catcgtcat accgtggct tcttcaaca 
      agacgttcc ctaggtttg caccaagga cattccgcg 
      cggatgatt tcaacgacc ctatccatt tagaacgta 
      tttccactt tgaatacgt tcactgtgt atgatttag 
      gggcaatta aacccaata ctagcgatg actatactt 
      cagattttt accgaatta atgaatggt acaaaaagt 
      ggttgcctg atcgtgttt ttgatagac atgacacca 
      aaacgactt atcagattt atatcagat atgcaaaca 
      atacgaatg aaacgttta cgtttcatg gaccacctg 
      gtgctgatg aaagtccag ggcctgttc aatggacaa 
      aaccgtatt ttgattgcg ggagatcta atagatggt 
      tggtggcgg ctgttgtgc caattgccg atatttatc 
      caaggcata cagggttta ggcatatag aatatccca 
      cttacactg ctgtttctg tggttgaaa tggactacg 
      aacgtatcg acatcaacc aatgtccac ccggtctag 
      ggaacaaag aaggtctca acatgtttg ctgacacag 
      cccgatgta aaaaagaaa caaccgaat gcgaaccga 
      ttcacggtt ggggcttcc ggagaggtg gttatcagt 
      gtcgttgca aacccggtt atcgtctcc ctatgcaag 
      tccgacgtc cctttttgg gagaaatag tcgaacgtg 
      caacggcag aacaatact acaacggtt ttgactgtc 
      ttaaaatcg gatgggttc aaaaattac cggttcagt 
      gggaaaaag caagttcgt cggtccgta accactacc 
      tcgataaat accgggagt atcgttatc ccgtctcag 
      gacctgagg ctttacacc aacccaaaa tcaacatcc 
      acaaatcac tcgatttca ttttgggca tgaataagg 
      ataattgca agaattaca acaaggacg atttaattt 
      tatacggcg gtgtcgaat acggcgctt atgagtttt 
      tccaaaacg aagccaaaa tggccttac gacttgcaa 
      atttcatta gtgcctttt tacaagtca atgacccta 
      ctgaaattt attcgggga aacgagtcc ctgatcgac 
      cgctaacag aagacgaaa tgattggcg aaactttgg 
      cccttgtta tgggcaata gccgaattt ggtcggctg 
      gtacatatt gggaacgaa acaaattca caaaccgca 
      cacttttcg caccatacg cctacaaaa cgcaactaa 
      acacgcgaa aattcgcag ttgaggact tggcccgac 
      tcaacaaat ctgacgaag tctatttga ataaaccct 
      ggtttttga aattgaaac aacgctggt tgaccaact 
      ttgacagtc tcgaaaaat attacatga aaataaaaa 
      ttcgcttta acgaaacgg gcgaaacga caagaaagt 
      acgaacgct atccaaact tctacagag gggcccaat 
      taaagcacg gcttatgga ccgaaccgt attacgatt 
      gcgatggta aaaaaccca tgtggaaga ttaaatacg 
      cggcaccgt ttttcggtt gggacagtc ttaaagtca 
      aactcgaat tcaagggcg tggtcaccg tatcaatgg 
      atttgttaa aacttgata taaaccaat gtcccgaca 
      gatattacg tcccaaacg ccttcaaaa acacacaca 
      agtgcgatt tgcgaaact catattgtg ttccgattt 
      tgggccgtg gattcgaaa caggcggct acaagtgtg 
      agtgtaagc aaggttacg aatatccgt ttgaagatc 
      tcatcactt attacgacg gtcaagtgt tggaagccg 
      aatttagta atttagttg ataataatg agacacgtt 
      ttgacatgt tcaagtgtc gcttagctg gcgctagca 
      gtgttcaag ttagtatcg ttacagttt tgttactga 
      tatttgtta gtttcggtt tgtatggca gaagttgaa 
      accaatgac ttgaaatcc aaggccttt caagtgcct 
      tattgatga tattttata gttttaccg tccattttc 
      agttgtagt tgtaggttt ttggtacaa aaaatattt 
      tgtatgtta tattgtgta cttgttgtg cacttaatc 
      gtactatag gccgtgttg caatttgtt gggcgatcg 
      tgagttgtg tacatgtac atatttttg atattcttt 
      ttattccta agtgcacaa ttgagcaag aaattgttc 
      aattaaccg attttttta ttactgttt tattaattt 
      ctccgtccg ttattttaa caatctgat tataataat 
      ttaagttta ataaacgat tttacaagg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY