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Gene information
General information
TC001340 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14778515..14779966
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001340
    iBeetle screen iB_09708

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001340 RA
      MNFVRFFLS LVFFSATSL ANLAELVGK KHETLVLLH 
      VLFRHGDRT PDKASLFPN DLYTEATYE PFGYSQLTT 
      KGKKTEYSI GKYLRRTYA DFIPEQYSP DVVYALSTN 
      VKRTKMSLQ LVLASLFPP LWGETFELG LGWQPVPFN 
      IEQGGNLIS VASGYCPNY ISKYYSYVL SDEAQKILA 
      VYTDLYAKL SRYSGMDII TPKDAANIY FTLKCEEDF 
      GLKLPQWAS EVYPQVLED ASAVDYELS TATPDLKKL 
      SAGFLLRKI IQDSLAKQN GTLPEKRKI FLYSAHEWN 
      VATMLRTLG VFYRHVPPF GATVFFEIH NIDGEFGLK 
      LYYQDYKQT NPKLLVIPG CTSFCPLDE LIKLMGDNL 
      PAEDDVCST TEELM
    CDS sequence
    • >TC001340 RA
      atgaacttt gttcgattc ttcctgtct ctcgtgttt 
      ttttcagct acgagtttg gcaaatctg gctgaactt 
      gtagggaaa aaacacgag actttggtg ttactccac 
      gtgttgttt agacacggt gaccgaaca cctgacaaa 
      gcaagtctt ttccccaat gatctctac actgaagca 
      acttacgaa ccgttcggt tacagccaa ctgaccact 
      aaagggaaa aagactgaa tacagtatt ggaaaatac 
      ttgcgaagg acttatgca gatttcatt ccggagcag 
      tacagccct gatgttgtt tacgccctt tccacaaac 
      gtgaaaagg actaaaatg tccctacag ttggtccta 
      gcgtccctt tttccgcct ctttggggc gaaaccttc 
      gagctaggt ttagggtgg caaccagta ccatttaat 
      atcgaacaa ggagggaat ttaatcagt gtagctagt 
      ggttactgc cctaactac attagcaaa tattacagt 
      tacgttttg tccgatgaa gcccaaaaa attctagcc 
      gtttacacg gacctatat gccaaactt agccgatat 
      tccggaatg gacatcata acaccaaag gacgcagct 
      aatatttat ttcaccctc aaatgtgaa gaggatttc 
      gggttgaaa ttgccacaa tgggcatca gaggtttac 
      ccccaagtc ttggaagac gcttcagct gttgactac 
      gaattgtcc acagctacc cctgacctt aaaaaatta 
      tctgccggg tttttgctg agaaaaatc atacaggat 
      agtttggcg aagcaaaat gggacttta ccggaaaaa 
      agaaaaatc tttctgtat tctgcacat gagtggaat 
      gttgcaacc atgttgagg actttgggt gtgttttac 
      agacatgtg cctccattc ggggccact gtgtttttc 
      gagattcac aacatcgat ggagaattt ggtttaaag 
      ttgtactac caggactac aagcaaacg aatcccaaa 
      ctgttggta attcctggt tgtaccagc ttttgtcct 
      cttgacgaa ctgatcaag ttaatgggt gataactta 
      cctgctgag gatgatgtg tgcagcact acggaagaa 
      cttatgtaa
    mRNA sequence
    • >TC001340 RA
      tgtaccatg aactttgtt cgattcttc ctgtctctc 
      gtgtttttt tcagctacg agtttggca aatctggct 
      gaacttgta gggaaaaaa cacgagact ttggtgtta 
      ctccacgtg ttgtttaga cacggtgac cgaacacct 
      gacaaagca agtcttttc cccaatgat ctctacact 
      gaagcaact tacgaaccg ttcggttac agccaactg 
      accactaaa gggaaaaag actgaatac agtattgga 
      aaatacttg cgaaggact tatgcagat ttcattccg 
      gagcagtac agccctgat gttgtttac gccctttcc 
      acaaacgtg aaaaggact aaaatgtcc ctacagttg 
      gtcctagcg tcccttttt ccgcctctt tggggcgaa 
      accttcgag ctaggttta gggtggcaa ccagtacca 
      tttaatatc gaacaagga gggaattta atcagtgta 
      gctagtggt tactgccct aactacatt agcaaatat 
      tacagttac gttttgtcc gatgaagcc caaaaaatt 
      ctagccgtt tacacggac ctatatgcc aaacttagc 
      cgatattcc ggaatggac atcataaca ccaaaggac 
      gcagctaat atttatttc accctcaaa tgtgaagag 
      gatttcggg ttgaaattg ccacaatgg gcatcagag 
      gtttacccc caagtcttg gaagacgct tcagctgtt 
      gactacgaa ttgtccaca gctacccct gaccttaaa 
      aaattatct gccgggttt ttgctgaga aaaatcata 
      caggatagt ttggcgaag caaaatggg actttaccg 
      gaaaaaaga aaaatcttt ctgtattct gcacatgag 
      tggaatgtt gcaaccatg ttgaggact ttgggtgtg 
      ttttacaga catgtgcct ccattcggg gccactgtg 
      tttttcgag attcacaac atcgatgga gaatttggt 
      ttaaagttg tactaccag gactacaag caaacgaat 
      cccaaactg ttggtaatt cctggttgt accagcttt 
      tgtcctctt gacgaactg atcaagtta atgggtgat 
      aacttacct gctgaggat gatgtgtgc agcactacg 
      gaagaactt atgtaaatt tgatagctg ttgtgcata 
      caataaggg aataaagga ataatacaa gaagttttt 
      tgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY