Status
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Gene information
General information
TC001338 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14768365..14775139
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001338
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09706

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001338 RA
      MMLGRKQSL KGEPVLADY GPDEAFNES ADIEWVNKR 
      WVRRLMRTC ALLSLASVS CNTPKTFER IPPMQGITF 
      EVDIVVTIL FTSEMVAKM HIRGILKGE KPYLKDHWC 
      QFDASMVFF LWISVILQV FEIMGTVPR FSYLSILRA 
      PRPLIMIRF LRVFLKFSM PKSRINQIF KRSSQQIYN 
      VTLFFLFFM SLYGLLGVQ FFGELKNHC VLNHTDLKH 
      ITINSLAIP DTFCSLDPT SGYQCPEGM KCMKLEGLS 
      RYIIGFNGF DEFVTSFFT VYQAASQEG WVFIMYRAI 
      DSLPGWRAV FYFSTMIFF LAWLVKNVF IAVITETFN 
      EIRVQFQQM WGVRGHIQN STASQILIG DDRGWKLVT 
      LDENKHGGL APQFCHTIL QSPHFRLIV MGVILANGI 
      VTATMRFKH DERPREVFY EKYYYMEVG FTILLDLET 
      IFKIWCLGW RSYWKHSIH KFELLLVVG TTIHVIPVF 
      YMSVFTYFQ VLRVVRLIK ASPLLEDFV YKIFGPGKK 
      LGSLIIFTM CLLIISSSI SMQLFCFLD FTKFETFAE 
      AFMSMFQIL TQEAWVEVM DETMLRTRS MLAPLVAVY 
      FILYHLFVT LIVLSLFVA VILDNLELD EDIKKLKQL 
      KYREQSAEI KETLPFRLR IFEKFPDSP LMVTLHKVP 
      SDFNLPKVR ESFMRQFVY ETEEDENSE GGIKKLSEE 
      TYDSKIIYR KQKPLKVLN NLPKVRVEN IKLKKTSVS 
      FIINDSNNQ RLMLGDSSM IPVPGTKGP LKPQGTVNS 
      MKQLRIDLK KFGSRSIRR SVRSGSIKL KQTYEHLME 
      NGDIGVNRV TSNRARPHD LDIKLLQAK RQQAEMRRN 
      QREEDLREN HPFFDTPLF AVPRESRFR KFCQLIVYA 
      RYDARLKDP LTGKERKVK YKSLHNFLG LVTYLDWVM 
      ITMTTLSCL SMMFETPYY RVMENVSLQ IAEYCFVIF 
      MSIELALKI LADGLFFTP KAYVKDVAA LLDVFIYLV 
      SLVFLCWMP KRVPPNSGA QLLMILRCL RPLRIFTLV 
      PHMRKVVYE LCRGFKEIL LVSTLLVLL MFIFASYGV 
      QLYGGKLAR CNDPSITQR KDCVGVFFR RVFVTKMKL 
      IPGENETYP SILVPRVWA NPRRFNFDN IGDAMLTLF 
      EVLSFKGWL DVRDVLIKA LGPAHAIYI HVYIFLGCM 
      IGLTLFVGV VIANYSENK GTALLTVDQ RRWCDLKKR 
      LKIAQPLHL PPRPDGKKF RAFIYDITQ NIKFKRYIA 
      MMVLINSAL LAVTWNKED DHTDILATV GMICTLLFL 
      VEVVMKNIA FTPRGYLQS RRNRYDLFV TVMGVIWIF 
      FHIMFKNDL TYFVGFMVV ILRFFTITG KHATLKMLM 
      LTVGVSVCK SFFIIFGMF LLVFFYALA GTIIFGTVK 
      YGEGIGRRA NFESPVTGV AMLFRIVTG EDWNKIMHD 
      CMIQPPYCT PADNYWQTD CGNFTGSLI YFCTFYVII 
      TYIVLNLLV AIIMENFSL FYSNEEDAL LSYADIRNF 
      QNTWNIVDI HQRGVIPVR RVKFILRLL KGRLEVDPQ 
      KDRLLFKHM CYELERLHN GEDVTFHDV INMLSYRSV 
      DIRKALQLE ELLAREEFE YIIEEEVAK QTIRTWLEG 
      CLKKIRSTN KQQNSLIAG LRATNEVIT MPETTEETK 
      NGAQDQEAE TEVKETEPR KQKLVTLPR SDSIGSSSG 
      RKYLAPTLS DPAARTEKE RVATKKKNN RPTVVSKNL 
      PHVNETNEA NRMPREVFN NKTTVPKAS NAMHEIRDW 
      WNEQLAYAS QSSDEE
    CDS sequence
    • >TC001338 RA
      atgatgttg gggagaaaa cagagcttg aaaggggag 
      cctgttttg gctgactat ggtccagat gaggcgttc 
      aatgagagc gctgatatc gaatgggtc aacaagaga 
      tgggtacgg agacttatg cgcacttgt gctttacta 
      agcctggct tctgtaagc tgcaacact cccaaaacc 
      ttcgagaga ataccgcca atgcaaggc ataactttc 
      gaagtcgat atcgtcgtc actatttta ttcacctcg 
      gaaatggtg gcgaaaatg cacataaga ggcattttg 
      aaaggcgaa aaaccctac ttgaaggac cactggtgt 
      cagtttgat gccagtatg gtttttttt ctatggatt 
      tcagtaatt ttgcaagtt tttgaaatc atggggacg 
      gtgccacgt ttcagttac ttatctatt ttaagagcg 
      cctcggcct ttgattatg atacgattt ctgcgcgtt 
      ttcttgaag ttttcgatg ccaaaatcg agaatcaac 
      caaattttc aagcgctcg agtcaacag atttacaac 
      gtgacctta tttttccta ttttttatg tccttgtat 
      ggtcttctc ggagtccag tttttcgga gaactcaaa 
      aatcactgc gttctcaac cataccgac ttaaaacac 
      attactatc aatagcctt gccattcct gacactttc 
      tgttctttg gaccccacc tcgggctac caatgccca 
      gaaggcatg aaatgtatg aaactagaa ggtttatcc 
      cgttacata atcgggttc aatggtttt gacgaattt 
      gttacaagt tttttcact gtgtaccaa gcagcctcg 
      caagaaggc tgggtcttt atcatgtac agagccata 
      gattctctg cccggatgg cgagctgtg ttttacttc 
      agcacaatg atttttttc ctggcttgg ttagtcaag 
      aacgttttc attgctgtg atcacggaa acgttcaac 
      gaaatcaga gtccagttt cagcaaatg tggggcgtt 
      agggggcac attcaaaac agtacagcg tcacaaatt 
      ttgattggt gatgacaga gggtggaag cttgtaaca 
      cttgacgaa aacaagcat gggggcctt gccccccaa 
      ttttgccac acgatttta caatccccc catttccgg 
      ctaatcgta atgggggtg attctcgca aatgggata 
      gtcacggct acaatgcgc ttcaaacat gacgaaaga 
      ccaagggaa gttttctac gaaaaatac tactacatg 
      gaggtcggt tttacaatt ctcctggac ttggagaca 
      attttcaag atttggtgc ctcggttgg aggagttac 
      tggaaacat tcgatccac aaatttgaa cttctccta 
      gtcgtgggg acgactatt cacgtcatt cctgtgttc 
      tacatgagt gtgttcacc tacttccaa gttttgaga 
      gttgtgcgg ttgatcaaa gcgtcgcct cttttggaa 
      gactttgtc tacaagatt tttgggcct gggaaaaaa 
      ctaggaagc ttgattata ttcaccatg tgtttgttg 
      atcatttcg tccagtatt tcaatgcaa ctgttctgt 
      tttttggat tttacgaaa tttgaaacc tttgccgaa 
      gcgttcatg tccatgttc cagattctg acccaggaa 
      gcttgggtc gaagtcatg gacgagaca atgttgcgg 
      acacggtca atgcttgca cctctagtc gcagtttac 
      tttattttg taccacctg ttcgtcact ctgatcgtg 
      ttaagtttg ttcgtagca gtcattctg gacaatttg 
      gagttggac gaagacatt aagaaacta aaacagctc 
      aaatatcgg gaacagagc gctgaaatt aaagaaacg 
      ttgcctttt cgtttacgg atttttgag aaattcccc 
      gatagtccc ttaatggtg acgctacat aaagtacca 
      tctgatttc aatttgccc aaagtgagg gaaagtttc 
      atgcgacag tttgtgtac gagacggaa gaagacgaa 
      aattcggaa ggtggcatc aagaaacta agcgaagag 
      acttacgat tctaaaatt atttatcgg aagcaaaag 
      ccactcaaa gttttgaac aatttgcca aaagtccgg 
      gtcgagaat attaaactg aaaaaaact tcagtctcg 
      ttcattatc aatgattca aataatcaa agattaatg 
      ttgggtgac tcgtccatg attccagtt ccgggcact 
      aaaggccct ctcaagcct caaggcact gttaacagt 
      atgaaacag ttacgtatt gatctgaag aaatttggt 
      tcacgatca attcggcga agtgttcgc agcggttcc 
      atcaaacta aaacaaacc tacgaacat ttaatggaa 
      aacggtgat attggagtg aatcgagtg acttcaaac 
      cgggcacga ccgcacgat cttgacatt aaattactg 
      caagccaag cgacaacaa gcggaaatg cggagaaac 
      caacgagag gaagatttg agggaaaac cacccgttt 
      ttcgacaca cccttattt gcagtcccg cgggaaagc 
      aggttcagg aaattctgc cagttgatt gtgtatgcc 
      aggtatgac gcccgcttg aaggacccc ttgactggc 
      aaagagcgc aaagtgaaa tacaaaagt ctccacaat 
      tttttgggt ttggtgact tatctggac tgggtcatg 
      ataacaatg acgacactg tcgtgtctc tccatgatg 
      ttcgaaaca ccgtactat cgcgtcatg gaaaatgtc 
      agtctccaa atcgccgaa tattgtttc gttattttc 
      atgagcata gaactagcg ctaaaaatc ctagccgac 
      gggcttttc ttcacaccc aaagcctac gtgaaagac 
      gtggctgcg ttattggac gttttcatc taccttgtg 
      agtctagtt tttctctgc tggatgcca aaaagggtc 
      ccacctaac tcaggggcc caacttttg atgatcctc 
      cgttgtttg cgtccgtta cgaattttc actctagta 
      ccacacatg aggaaagtg gtttatgag ctttgccgc 
      ggcttcaag gagattctc ttagtatca acacttttg 
      gtacttttg atgtttatt tttgcaagt tacggggtc 
      cagctttac ggtggtaaa ttagcacgg tgtaatgac 
      cccagcatt acgcaaagg aaggactgt gttggggtt 
      tttttccgg agagttttc gtaacgaaa atgaagcta 
      ataccgggc gaaaacgaa acgtacccc tcgattctt 
      gtaccccga gtttgggca aacccacga cgtttcaat 
      tttgataat attggggac gcgatgttg actctattc 
      gaagttttg tcgtttaaa ggatggctg gacgttaga 
      gacgtgcta attaaagcc ctaggaccc gcccacgct 
      atttacatt catgtttac atatttctg ggctgcatg 
      attggtttg acacttttt gtgggcgta gttattgca 
      aattattcc gaaaataaa ggcacagct ttgctaact 
      gtagatcag aggagatgg tgcgatttg aagaagcga 
      ctaaaaatc gcccaaccg ttgcacctg ccaccaaga 
      ccagacggg aaaaaattc agagctttc atctatgat 
      atcacccaa aatattaag tttaaacgc tacatagcc 
      atgatggtt ttgatcaat agtgcccta ctggcggtc 
      acgtggaat aaggaagac gaccacaca gacatcctt 
      gcaactgtc ggaatgata tgtacgttg ttgttcttg 
      gtcgaagtt gtgatgaaa aatatagct ttcaccccg 
      agaggctat ttacagtcg aggagaaac agatacgac 
      cttttcgtc acagttatg ggagtaatt tggattttc 
      ttccacata atgtttaaa aacgatttg acgtatttt 
      gttggtttc atggtcgta attttacga tttttcacc 
      ataactggg aaacacgcc actcttaaa atgttgatg 
      cttacggtc ggtgtttca gtttgcaaa agtttcttc 
      attattttt ggaatgttt ctgcttgtg tttttctac 
      gctttggca gggactatt atttttgga acggtgaaa 
      tacggggag ggaattggg aggagagct aatttcgaa 
      tcacctgta acaggggtt gcaatgtta tttagaatc 
      gttactggg gaagactgg aataagata atgcatgat 
      tgtatgatt caacccccg tattgtacc ccagctgat 
      aattactgg cagacggat tgtggcaat tttactggg 
      tctttgatt tatttctgt actttttac gtgattatt 
      acctacatt gtgctcaat ttgttagtg gctattatc 
      atggagaac ttttcttta ttttattca aacgaagaa 
      gacgcttta ctgtcgtac gctgatatt cggaacttc 
      caaaacact tggaatatt gttgatatt catcaaaga 
      ggtgttata ccagtacga cgggtcaaa tttattcta 
      cggctgcta aaaggcaga ttggaagta gatccacaa 
      aaagacagg cttttattt aagcacatg tgttacgaa 
      ctggagcgc ttacacaat ggggaagat gttacgttt 
      cacgacgtt atcaatatg ttgtcgtac aggtcagtt 
      gacatccga aaagccctc caactcgaa gaattacta 
      gctcgcgaa gaattcgaa tacattata gaagaagaa 
      gttgcaaaa caaaccata aggacatgg cttgaaggc 
      tgcctcaaa aaaatcaga tccactaat aagcaacaa 
      aacagtcta atagctgga ttacgcgca accaacgaa 
      gttataacc atgcctgaa acaacagaa gagaccaaa 
      aacggggct caagaccaa gaagctgaa actgaagtt 
      aaggaaact gaacctagg aagcaaaaa cttgtcacg 
      cttccgcgt tccgacagt attgggagc agttcaggc 
      cggaaatat ttagcaccg actttgtcc gatcctgcg 
      gcacggact gaaaaagaa cgagttgcc accaaaaag 
      aagaataat cgaccaact gtcgtaagc aagaatctc 
      ccccatgtt aatgaaacc aacgaagct aatcgaatg 
      cccagggag gtgtttaat aataaaacg actgtgccc 
      aaagcttca aacgccatg catgagatt agggactgg 
      tggaatgag caattggct tatgcgtcg cagtccagt 
      gatgaggaa taa
    mRNA sequence
    • >TC001338 RA
      tattggtag aagatattg atttttgaa tcagagcgg 
      ggctccaga ttcagttca agcagcatc cctgagctt 
      gaaaggtcg acaaatgta acaaaaact ttgcgcaaa 
      atggtgtgt ttgaccact gacgccacc ggaatttat 
      taaatggcc acaaaccca atattttaa caattatgg 
      taattatgg tttagttat tatttgttc aatatgatg 
      ttggggaga aaacagagc ttgaaaggg gagcctgtt 
      ttggctgac tatggtcca gatgaggcg ttcaatgag 
      agcgctgat atcgaatgg gtcaacaag agatgggta 
      cggagactt atgcgcact tgtgcttta ctaagcctg 
      gcttctgta agctgcaac actcccaaa accttcgag 
      agaataccg ccaatgcaa ggcataact ttcgaagtc 
      gatatcgtc gtcactatt ttattcacc tcggaaatg 
      gtggcgaaa atgcacata agaggcatt ttgaaaggc 
      gaaaaaccc tacttgaag gaccactgg tgtcagttt 
      gatgccagt atggttttt tttctatgg atttcagta 
      attttgcaa gtttttgaa atcatgggg acggtgcca 
      cgtttcagt tacttatct attttaaga gcgcctcgg 
      cctttgatt atgatacga tttctgcgc gttttcttg 
      aagttttcg atgccaaaa tcgagaatc aaccaaatt 
      ttcaagcgc tcgagtcaa cagatttac aacgtgacc 
      ttatttttc ctatttttt atgtccttg tatggtctt 
      ctcggagtc cagtttttc ggagaactc aaaaatcac 
      tgcgttctc aaccatacc gacttaaaa cacattact 
      atcaatagc cttgccatt cctgacact ttctgttct 
      ttggacccc acctcgggc taccaatgc ccagaaggc 
      atgaaatgt atgaaacta gaaggttta tcccgttac 
      ataatcggg ttcaatggt tttgacgaa tttgttaca 
      agttttttc actgtgtac caagcagcc tcgcaagaa 
      ggctgggtc tttatcatg tacagagcc atagattct 
      ctgcccgga tggcgagct gtgttttac ttcagcaca 
      atgattttt ttcctggct tggttagtc aagaacgtt 
      ttcattgct gtgatcacg gaaacgttc aacgaaatc 
      agagtccag tttcagcaa atgtggggc gttaggggg 
      cacattcaa aacagtaca gcgtcacaa attttgatt 
      ggtgatgac agagggtgg aagcttgta acacttgac 
      gaaaacaag catgggggc cttgccccc caattttgc 
      cacacgatt ttacaatcc ccccatttc cggctaatc 
      gtaatgggg gtgattctc gcaaatggg atagtcacg 
      gctacaatg cgcttcaaa catgacgaa agaccaagg 
      gaagttttc tacgaaaaa tactactac atggaggtc 
      ggttttaca attctcctg gacttggag acaattttc 
      aagatttgg tgcctcggt tggaggagt tactggaaa 
      cattcgatc cacaaattt gaacttctc ctagtcgtg 
      gggacgact attcacgtc attcctgtg ttctacatg 
      agtgtgttc acctacttc caagttttg agagttgtg 
      cggttgatc aaagcgtcg cctcttttg gaagacttt 
      gtctacaag atttttggg cctgggaaa aaactagga 
      agcttgatt atattcacc atgtgtttg ttgatcatt 
      tcgtccagt atttcaatg caactgttc tgttttttg 
      gattttacg aaatttgaa acctttgcc gaagcgttc 
      atgtccatg ttccagatt ctgacccag gaagcttgg 
      gtcgaagtc atggacgag acaatgttg cggacacgg 
      tcaatgctt gcacctcta gtcgcagtt tactttatt 
      ttgtaccac ctgttcgtc actctgatc gtgttaagt 
      ttgttcgta gcagtcatt ctggacaat ttggagttg 
      gacgaagac attaagaaa ctaaaacag ctcaaatat 
      cgggaacag agcgctgaa attaaagaa acgttgcct 
      tttcgttta cggattttt gagaaattc cccgatagt 
      cccttaatg gtgacgcta cataaagta ccatctgat 
      ttcaatttg cccaaagtg agggaaagt ttcatgcga 
      cagtttgtg tacgagacg gaagaagac gaaaattcg 
      gaaggtggc atcaagaaa ctaagcgaa gagacttac 
      gattctaaa attatttat cggaagcaa aagccactc 
      aaagttttg aacaatttg ccaaaagtc cgggtcgag 
      aatattaaa ctgaaaaaa acttcagtc tcgttcatt 
      atcaatgat tcaaataat caaagatta atgttgggt 
      gactcgtcc atgattcca gttccgggc actaaaggc 
      cctctcaag cctcaaggc actgttaac agtatgaaa 
      cagttacgt attgatctg aagaaattt ggttcacga 
      tcaattcgg cgaagtgtt cgcagcggt tccatcaaa 
      ctaaaacaa acctacgaa catttaatg gaaaacggt 
      gatattgga gtgaatcga gtgacttca aaccgggca 
      cgaccgcac gatcttgac attaaatta ctgcaagcc 
      aagcgacaa caagcggaa atgcggaga aaccaacga 
      gaggaagat ttgagggaa aaccacccg tttttcgac 
      acaccctta tttgcagtc ccgcgggaa agcaggttc 
      aggaaattc tgccagttg attgtgtat gccaggtat 
      gacgcccgc ttgaaggac cccttgact ggcaaagag 
      cgcaaagtg aaatacaaa agtctccac aattttttg 
      ggtttggtg acttatctg gactgggtc atgataaca 
      atgacgaca ctgtcgtgt ctctccatg atgttcgaa 
      acaccgtac tatcgcgtc atggaaaat gtcagtctc 
      caaatcgcc gaatattgt ttcgttatt ttcatgagc 
      atagaacta gcgctaaaa atcctagcc gacgggctt 
      ttcttcaca cccaaagcc tacgtgaaa gacgtggct 
      gcgttattg gacgttttc atctacctt gtgagtcta 
      gtttttctc tgctggatg ccaaaaagg gtcccacct 
      aactcaggg gcccaactt ttgatgatc ctccgttgt 
      ttgcgtccg ttacgaatt ttcactcta gtaccacac 
      atgaggaaa gtggtttat gagctttgc cgcggcttc 
      aaggagatt ctcttagta tcaacactt ttggtactt 
      ttgatgttt atttttgca agttacggg gtccagctt 
      tacggtggt aaattagca cggtgtaat gaccccagc 
      attacgcaa aggaaggac tgtgttggg gtttttttc 
      cggagagtt ttcgtaacg aaaatgaag ctaataccg 
      ggcgaaaac gaaacgtac ccctcgatt cttgtaccc 
      cgagtttgg gcaaaccca cgacgtttc aattttgat 
      aatattggg gacgcgatg ttgactcta ttcgaagtt 
      ttgtcgttt aaaggatgg ctggacgtt agagacgtg 
      ctaattaaa gccctagga cccgcccac gctatttac 
      attcatgtt tacatattt ctgggctgc atgattggt 
      ttgacactt tttgtgggc gtagttatt gcaaattat 
      tccgaaaat aaaggcaca gctttgcta actgtagat 
      cagaggaga tggtgcgat ttgaagaag cgactaaaa 
      atcgcccaa ccgttgcac ctgccacca agaccagac 
      gggaaaaaa ttcagagct ttcatctat gatatcacc 
      caaaatatt aagtttaaa cgctacata gccatgatg 
      gttttgatc aatagtgcc ctactggcg gtcacgtgg 
      aataaggaa gacgaccac acagacatc cttgcaact 
      gtcggaatg atatgtacg ttgttgttc ttggtcgaa 
      gttgtgatg aaaaatata gctttcacc ccgagaggc 
      tatttacag tcgaggaga aacagatac gaccttttc 
      gtcacagtt atgggagta atttggatt ttcttccac 
      ataatgttt aaaaacgat ttgacgtat tttgttggt 
      ttcatggtc gtaatttta cgatttttc accataact 
      gggaaacac gccactctt aaaatgttg atgcttacg 
      gtcggtgtt tcagtttgc aaaagtttc ttcattatt 
      tttggaatg tttctgctt gtgtttttc tacgctttg 
      gcagggact attattttt ggaacggtg aaatacggg 
      gagggaatt gggaggaga gctaatttc gaatcacct 
      gtaacaggg gttgcaatg ttatttaga atcgttact 
      ggggaagac tggaataag ataatgcat gattgtatg 
      attcaaccc ccgtattgt accccagct gataattac 
      tggcagacg gattgtggc aattttact gggtctttg 
      atttatttc tgtactttt tacgtgatt attacctac 
      attgtgctc aatttgtta gtggctatt atcatggag 
      aacttttct ttattttat tcaaacgaa gaagacgct 
      ttactgtcg tacgctgat attcggaac ttccaaaac 
      acttggaat attgttgat attcatcaa agaggtgtt 
      ataccagta cgacgggtc aaatttatt ctacggctg 
      ctaaaaggc agattggaa gtagatcca caaaaagac 
      aggctttta tttaagcac atgtgttac gaactggag 
      cgcttacac aatggggaa gatgttacg tttcacgac 
      gttatcaat atgttgtcg tacaggtca gttgacatc 
      cgaaaagcc ctccaactc gaagaatta ctagctcgc 
      gaagaattc gaatacatt atagaagaa gaagttgca 
      aaacaaacc ataaggaca tggcttgaa ggctgcctc 
      aaaaaaatc agatccact aataagcaa caaaacagt 
      ctaatagct ggattacgc gcaaccaac gaagttata 
      accatgcct gaaacaaca gaagagacc aaaaacggg 
      gctcaagac caagaagct gaaactgaa gttaaggaa 
      actgaacct aggaagcaa aaacttgtc acgcttccg 
      cgttccgac agtattggg agcagttca ggccggaaa 
      tatttagca ccgactttg tccgatcct gcggcacgg 
      actgaaaaa gaacgagtt gccaccaaa aagaagaat 
      aatcgacca actgtcgta agcaagaat ctcccccat 
      gttaatgaa accaacgaa gctaatcga atgcccagg 
      gaggtgttt aataataaa acgactgtg cccaaagct 
      tcaaacgcc atgcatgag attagggac tggtggaat 
      gagcaattg gcttatgcg tcgcagtcc agtgatgag 
      gaataatta ttgtttacg aatatatta aatgtttta 
      ggaagtgtt attttatta ataaagata tatgcaagt 
      a
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