Status
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Gene information
General information
TC000208 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14698403..14702243
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000208
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09705

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000208 RA
      MDEELRCPI CHEFRQDNQ HNQTLNHRF YFAKWSYNE 
      YRKALISDR HGIRLNVAP EPINNFQID FKYYKGKYT 
      TKVTPEQLR LHNSTITYK CTIQNAKKD DPVYITSTD 
      IIHPSSYFT TKHPHVFGD DERSFILLQ PGEQYTFKV 
      VFQTDILAR ASYKIPLTC DIQTESKKE SFTIVRSFL 
      TLVQSQGEK DEKKGKSPF TSQPWKEVT KTVPTTKEL 
      PYKDKYPIP GKYIKILTY GIGAYSGMT VTEEETLNT 
      LSRLVAQLT RENYNRFWH VVLWLEERA QALMLMKFN 
      MQGVTMSLR DDDVLILTV PGLAEKRPS VIVGDFIKI 
      RITNDHTAY KGFVGAVNE KTVEISHVD NELLEYIRE 
      NPDIELDVA FMLSRLAFE RMHAAVDKI VSSGLVAKL 
      FPVERRLPR SANAHNLDN STLFNKTIA QNPEQKNAV 
      DKIVNNIQD IPYIVFGPP GTGKTVTIV EAILQIKKR 
      TKKRILVCA PANSACDML ATKLMPHCT TEELIRINS 
      TTRERTTMT EDLKEYSNM EDDEFTRVV IDRLLSYRI 
      VVTTLTLIG RYATGYRPD CVFIDEAAQ ASEPESDIA 
      IALAGVGKQ VVLAGDPKQ LGPMVTKSA EKFGLGKSL 
      LERLMEFEV YQLNRTTRN YDTNFITML RLNFRSHPR 
      ILEIPNNLF YDDLLKPVS RIALQDPIA NIPIYAKIV 
      SSCNNNGQA IEMCAISAK EQKEGSSPS FFNLPEAEM 
      VGKYVKALV NLPLAEAYK VRLDQIGVV TPYKRQVFK 
      IKEELMYKG YQEVEVGTT ESFQGREKR IIIISTVRA 
      QHSLLLHDR KYDLGFVKN DQRFNVAIT RAISKLIVI 
      GNTPVLRTD HKWNRFIQA CEEHNTCFG HQLENSSNV 
      FKTGVVKRT KRFKSKDDQ YHR
    CDS sequence
    • >TC000208 RA
      atggatgag gaattacgg tgtcccatt tgccatgag 
      tttaggcaa gacaaccag cacaatcaa acactgaat 
      catcgattt tatttcgcg aaatggagc tataatgag 
      tatagaaaa gctctgata agtgatagg cacggtatt 
      cgcctaaat gtagcccca gaacccata aataacttt 
      cagattgat ttcaaatat tacaaggga aagtacaca 
      acgaaggtc actccagag cagctaaga ttgcataac 
      agcacaata acatacaaa tgcacgatt caaaatgcg 
      aaaaaagac gaccctgtt tacattaca agcacagac 
      ataatacat ccttcgtct tattttaca actaaacat 
      ccacatgtc tttggcgat gacgaacgt agttttata 
      ttgcttcag ccaggtgaa cagtatacc tttaaagtg 
      gtatttcaa acagacata ttagccaga gccagctac 
      aaaatccct ctaacttgc gatatccaa accgagagc 
      aaaaaggaa agcttcaca atagtgcgg tcttttctc 
      acattagtt caatcacaa ggcgaaaaa gatgaaaag 
      aagggtaag agtccattt acatcccag ccttggaaa 
      gaggtcacc aagaccgtt ccaacaact aaagaactc 
      ccctataaa gacaaatat ccaattcct ggaaaatat 
      atcaagatt ttgacatac ggtattgga gcttatagt 
      gggatgacg gtaactgaa gaggaaact ttgaatact 
      ctcagccga cttgttgcc cagttaaca agagagaat 
      tacaaccgt ttttggcac gtggtcttg tggttggaa 
      gaaagagcg caagctctg atgctcatg aagttcaac 
      atgcaagga gtgacaatg agtttaaga gatgacgac 
      gtcttaatt cttacggta cctggttta gctgaaaaa 
      cggccgtct gtcatagtg ggtgatttt ataaagatc 
      aggataaca aacgaccac actgcctac aagggattt 
      gttggagca gttaacgaa aaaactgtg gaaatatcg 
      cacgtggac aatgaatta ctggagtac attcgcgaa 
      aacccagac attgaatta gatgttgcg tttatgttg 
      agtcggtta gccttcgag agaatgcat gcggccgtc 
      gataaaatc gtatcatca ggcttagtc gcgaaactt 
      tttcctgtt gagagaaga cttccaagg tcggcaaat 
      gcacataac ttagataac tcaacattg tttaataag 
      acgattgca caaaacccg gaacaaaaa aatgcagta 
      gataaaatc gtaaataac attcaggac attccttac 
      atcgttttt gggcctcct gggactggc aaaaccgtg 
      actatcgtc gaagccatc ttacagatt aaaaaacgc 
      acaaaaaag cggatactg gtgtgcgcc ccagcaaat 
      tccgcgtgt gacatgtta gcaacaaaa cttatgccg 
      cactgtacc acagaggaa ttgatccga atcaattct 
      accactagg gagagaacc actatgacc gaggatttg 
      aaagaatac agtaatatg gaagacgac gaatttaca 
      agagttgta attgatcgg ctcctctcg taccgaatt 
      gtggttaca accctaact cttatcggt cgctacgcc 
      acaggctat cgccctgac tgtgttttc atcgacgag 
      gcggctcaa gccagcgaa cctgaatcc gatattgca 
      attgcactt gctggcgtt ggtaaacaa gtggtccta 
      gcaggagat cccaaacag ttggggcca atggtaaca 
      aaatcagcc gaaaagttc ggtttgggg aaatcacta 
      ttggaacga ttgatggaa tttgaagtc taccaactc 
      aaccgcact accagaaac tacgacacg aattttata 
      accatgctc aggttaaat ttcaggtca catccccga 
      attttagaa attccgaac aatttgttc tacgatgat 
      ttattaaag ccagtgtcc agaatcgca ctacaggac 
      ccaatcgca aacataccc atttatgca aaaatagta 
      tcatcgtgt aacaataat ggtcaagcg attgaaatg 
      tgtgccatt agtgcgaaa gaacaaaaa gaagggtcg 
      tcgccaagt tttttcaat ttaccggaa gctgaaatg 
      gttgggaaa tatgtaaag gctttagta aacctaccc 
      cttgcggaa gcgtacaaa gtgagatta gatcaaatc 
      ggagtggtc acaccgtac aaacgtcaa gtttttaaa 
      atcaaagaa gaattaatg tataaagga tatcaagaa 
      gtggaggtt ggcacaact gaatctttc caaggccgc 
      gagaaacgc atcataata attagtaca gtgagagcg 
      caacattcc ttactatta catgaccgt aaatatgat 
      ttgggtttt gtgaagaat gatcagcga ttcaacgta 
      gcaatcaca cgagcaatc agtaaatta attgtcatt 
      ggaaacaca ccagtcttg aggactgac cacaagtgg 
      aataggttt atacaggca tgcgaggaa cataacact 
      tgctttgga catcaattg gaaaactcc agtaatgtg 
      ttcaagact ggggtcgtt aaaaggaca aagcgattc 
      aagagtaaa gatgatcaa taccataga taa
    mRNA sequence
    • >TC000208 RA
      tgacattga atttacata aagaaaaac ttgcacatg 
      agcgcagtt atcgactta gcggtagag tatgcatga 
      aataattgt agttagtgt gaactaagt gaaatttca 
      cgtagcggt tgtaacttg tttcgtttc attaagaat 
      gtatttaga ttgtttgag agtgaacat tgtgatatc 
      tttcatttt tatcgcact tattttgtt atcacgatt 
      cgactaaat cgttcgtga tcgacttta gtttgcgtc 
      acacaatat gtagtgttt gttagtcca tgtatgtac 
      ttcacctgt gaaagagag ggattcccc tgagtcctt 
      taggcgcaa tggatgagg aattacggt gtcccattt 
      gccatgagt ttaggcaag acaaccagc acaatcaaa 
      cactgaatc atcgatttt atttcgcga aatggagct 
      ataatgagt atagaaaag ctctgataa gtgataggc 
      acggtattc gcctaaatg tagccccag aacccataa 
      ataactttc agattgatt tcaaatatt acaagggaa 
      agtacacaa cgaaggtca ctccagagc agctaagat 
      tgcataaca gcacaataa catacaaat gcacgattc 
      aaaatgcga aaaaagacg accctgttt acattacaa 
      gcacagaca taatacatc cttcgtctt attttacaa 
      ctaaacatc cacatgtct ttggcgatg acgaacgta 
      gttttatat tgcttcagc caggtgaac agtatacct 
      ttaaagtgg tatttcaaa cagacatat tagccagag 
      ccagctaca aaatccctc taacttgcg atatccaaa 
      ccgagagca aaaaggaaa gcttcacaa tagtgcggt 
      cttttctca cattagttc aatcacaag gcgaaaaag 
      atgaaaaga agggtaaga gtccattta catcccagc 
      cttggaaag aggtcacca agaccgttc caacaacta 
      aagaactcc cctataaag acaaatatc caattcctg 
      gaaaatata tcaagattt tgacatacg gtattggag 
      cttatagtg ggatgacgg taactgaag aggaaactt 
      tgaatactc tcagccgac ttgttgccc agttaacaa 
      gagagaatt acaaccgtt tttggcacg tggtcttgt 
      ggttggaag aaagagcgc aagctctga tgctcatga 
      agttcaaca tgcaaggag tgacaatga gtttaagag 
      atgacgacg tcttaattc ttacggtac ctggtttag 
      ctgaaaaac ggccgtctg tcatagtgg gtgatttta 
      taaagatca ggataacaa acgaccaca ctgcctaca 
      agggatttg ttggagcag ttaacgaaa aaactgtgg 
      aaatatcgc acgtggaca atgaattac tggagtaca 
      ttcgcgaaa acccagaca ttgaattag atgttgcgt 
      ttatgttga gtcggttag ccttcgaga gaatgcatg 
      cggccgtcg ataaaatcg tatcatcag gcttagtcg 
      cgaaacttt ttcctgttg agagaagac ttccaaggt 
      cggcaaatg cacataact tagataact caacattgt 
      ttaataaga cgattgcac aaaacccgg aacaaaaaa 
      atgcagtag ataaaatcg taaataaca ttcaggaca 
      ttccttaca tcgtttttg ggcctcctg ggactggca 
      aaaccgtga ctatcgtcg aagccatct tacagatta 
      aaaaacgca caaaaaagc ggatactgg tgtgcgccc 
      cagcaaatt ccgcgtgtg acatgttag caacaaaac 
      ttatgccgc actgtacca cagaggaat tgatccgaa 
      tcaattcta ccactaggg agagaacca ctatgaccg 
      aggatttga aagaataca gtaatatgg aagacgacg 
      aatttacaa gagttgtaa ttgatcggc tcctctcgt 
      accgaattg tggttacaa ccctaactc ttatcggtc 
      gctacgcca caggctatc gccctgact gtgttttca 
      tcgacgagg cggctcaag ccagcgaac ctgaatccg 
      atattgcaa ttgcacttg ctggcgttg gtaaacaag 
      tggtcctag caggagatc ccaaacagt tggggccaa 
      tggtaacaa aatcagccg aaaagttcg gtttgggga 
      aatcactat tggaacgat tgatggaat ttgaagtct 
      accaactca accgcacta ccagaaact acgacacga 
      attttataa ccatgctca ggttaaatt tcaggtcac 
      atccccgaa ttttagaaa ttccgaaca atttgttct 
      acgatgatt tattaaagc cagtgtcca gaatcgcac 
      tacaggacc caatcgcaa acataccca tttatgcaa 
      aaatagtat catcgtgta acaataatg gtcaagcga 
      ttgaaatgt gtgccatta gtgcgaaag aacaaaaag 
      aagggtcgt cgccaagtt ttttcaatt taccggaag 
      ctgaaatgg ttgggaaat atgtaaagg ctttagtaa 
      acctacccc ttgcggaag cgtacaaag tgagattag 
      atcaaatcg gagtggtca caccgtaca aacgtcaag 
      tttttaaaa tcaaagaag aattaatgt ataaaggat 
      atcaagaag tggaggttg gcacaactg aatctttcc 
      aaggccgcg agaaacgca tcataataa ttagtacag 
      tgagagcgc aacattcct tactattac atgaccgta 
      aatatgatt tgggttttg tgaagaatg atcagcgat 
      tcaacgtag caatcacac gagcaatca gtaaattaa 
      ttgtcattg gaaacacac cagtcttga ggactgacc 
      acaagtgga ataggttta tacaggcat gcgaggaac 
      ataacactt gctttggac atcaattgg aaaactcca 
      gtaatgtgt tcaagactg gggtcgtta aaaggacaa 
      agcgattca agagtaaag atgatcaat accatagat 
      aatatattt tttataatc cgatacttt taataaact 
      ttaccagtg aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY