Status
Please wait ...
Gene information
General information
TC001327 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14541795..14544556
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001327
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09704

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001327 RA
      MIIFENCDL DSACHAVVN AVWGFTGML PWSVRNIFV 
      QENVFETFI QKLKHKVGE IRVGQSDQK NLDISSSLD 
      NKTLQKLVK IIERAKDEG VEVYQPNNN AIPTLFIGG 
      NVFHNNVIV EDAIKVPIV TLTAFRTIN EAAALSNNT 
      KQGLAASVW TESTSLANE IIEKLKVGT VWINSHGLF 
      APDIPFSPF KSSGNAFFG GTEGFYEYV DTKNLKTET 
      PSQVRNATS MEKAINEAK KAQNAWFKL DHFERTKIL 
      QTVAHEIQN KVWGLPDNW INNWVTLIH DCLSNHSLN 
      IVTKKQSFN VVTMREPCG LIAIEISDD KETHNKKMI 
      IAALIEGNS VIILNGAKD TSDFYTFVA KLLPKGVLT 
      LVEHSQEAT KIAALHKEL NVYFGERNK VFGSLPLKT 
      SSKFKVVQT DDWNCVRNK VTLVKSVWF NKGTSV
    • >TC001327 RB
      MGDIGDPKH SVIKQIFET MSYGDDFEN LTEATEWLK 
      TKENRIFLH IDKKDETPN GEPLSIKNI NNKESLCEI 
      YVPEAATID AVLKKLGGA KNAWAEVAP LKRAEIIHK 
      FGGEICKKQ DLFTQLEVA VRGILSKDT RKSLPLLTD 
      WLQYYSSFS VKCNSKNSS WKPEGLAVA ILSDVNSLA 
      TFGYILGPC LAAGYNVVL QTGPQLSLV ASLLIEIAI 
      QAGIPDDTI RLIPGDVDF NIFLIHPQV SVISIFDDL 
      YKEKYLIAN HFNKKLINF YSSGIPMII FENCDLDSA 
      CHAVVNAVW GFTGMLPWS VRNIFVQEN VFETFIQKL 
      KHKVGEIRV GQSDQKNLD ISSSLDNKT LQKLVKIIE 
      RAKDEGVEV YQPNNNAIP TLFIGGNVF HNNVIVEDA 
      IKVPIVTLT AFRTINEAA ALSNNTKQG LAASVWTES 
      TSLANEIIE KLKVGTVWI NSHGLFAPD IPFSPFKSS 
      GNAFFGGTE GFYEYVDTK NLKTETPSQ VRNATSMEK 
      AINEAKKAQ NAWFKLDHF ERTKILQTV AHEIQNKVW 
      GLPDNWINN WVTLIHDCL SNHSLNIVT KKQSFNVVT 
      MREPCGLIA IEISDDKET HNKKMIIAA LIEGNSVII 
      LNGAKDTSD FYTFVAKLL PKGVLTLVE HSQEATKIA 
      ALHKELNVY FGERNKVFG SLPLKTSSK FKVVQTDDW 
      NCVRNKVTL VKSVWFNKG TSV
    CDS sequence
    • >TC001327 RA
      atgataatt tttgaaaac tgtgacttg gattcagcg 
      tgtcatgcg gtggtcaat gcagtttgg gggttcacc 
      ggaatgctg ccttggtcc gtacggaat atttttgtt 
      caggaaaac gttttcgaa acatttatc caaaaattg 
      aaacacaaa gttggtgaa ataagagtg ggacaaagc 
      gaccaaaaa aatttagat atttcgtca tcacttgat 
      aataaaacg ttgcaaaaa ttggtgaaa atcattgaa 
      agggcgaag gatgagggc gtggaagtg taccaacca 
      aacaataac gcaattccg actctattc atcggaggc 
      aacgttttc cacaataat gttatagtt gaggatgct 
      attaaagtc ccaattgta actttaaca gcttttcga 
      acgattaat gaagccgct gcattgtca aacaacact 
      aagcaaggc ctagcggct tcagtatgg acggaaagt 
      acgagttta gcaaacgaa ataatcgaa aaactcaag 
      gtcggaaca gtttggatc aattctcat ggtttattc 
      gcccccgat attcccttt tcaccgttc aaaagcagc 
      ggaaacgct ttcttcggc ggaactgaa ggattctac 
      gaatacgtt gatacaaaa aacttgaaa accgagaca 
      ccttcacaa gtgcgaaac gctaccagc atggaaaaa 
      gccataaat gaggcaaaa aaggcccaa aacgcctgg 
      ttcaaactt gaccacttt gaacgaacg aaaattctt 
      caaacagtg gctcacgaa atccaaaac aaagtatgg 
      ggtttaccc gacaattgg ataaataat tgggtcacc 
      ctgatccac gattgcctt tcaaatcac tcgttaaat 
      attgttacg aaaaaacaa agcttcaat gttgtaaca 
      atgcgagaa ccttgcgga cttatcgcg attgaaatt 
      tccgacgat aaagaaacg cataacaaa aaaatgata 
      attgcggct ttaattgaa ggcaattct gttattatt 
      ctcaatggt gccaaagac acgagtgat ttttacaca 
      ttcgttgcg aaactgctc ccaaagggc gttctaacg 
      ctcgttgaa cattcacaa gaggctacg aaaattgca 
      gcgttacat aaggagctg aatgtttat tttggggaa 
      aggaacaaa gtttttggc tccttgccg ctaaaaacc 
      tcaagtaaa tttaaagtg gtgcaaacg gacgactgg 
      aattgcgtc cgcaacaaa gtcactctg gttaaaagt 
      gtttggttc aataaggga acatctgtt tga
    • >TC001327 RB
      atgggcgat attggggac cctaagcat tccgtaatt 
      aaacaaata tttgaaaca atgagttat ggagacgat 
      ttcgaaaac ctgactgaa gcgacagag tggctcaag 
      accaaagaa aatcgcata tttctccat attgataaa 
      aaagatgaa acaccaaat ggagaaccc ttatcaatt 
      aaaaatatc aacaacaaa gagagttta tgtgagatt 
      tatgtccct gaagcagct acaattgac gcagttttg 
      aaaaagtta gggggggct aaaaatgca tgggctgag 
      gtcgccccc ttgaaacga gcagaaatc attcacaaa 
      ttcgggggc gagatttgc aagaaacag gatttattc 
      acacagctt gaagttgca gttcgtggc attctcagt 
      aaagacact agaaaaagc ttaccgtta cttaccgac 
      tggttgcaa tactattcc agcttcagt gtaaagtgt 
      aattcgaaa aattcttca tggaaaccc gagggctta 
      gccgtggca attttatca gatgtgaac tcgctagcg 
      acctttggc tacatactg gggccttgt ttagccgca 
      ggttacaat gtagtgctc cagactggt ccgcaactt 
      tctctagtt gcgtccctt ttgattgag attgcaatt 
      caagcggga ataccagac gacactatt cgattaata 
      ccgggggat gtggacttt aatatattt ctaatccac 
      ccccaagtg tccgtaatt tcaattttt gatgatctt 
      tacaaagaa aaatattta atagcaaac cattttaat 
      aagaaattg atcaatttt tattcgtcg ggcattcca 
      atgataatt tttgaaaac tgtgacttg gattcagcg 
      tgtcatgcg gtggtcaat gcagtttgg gggttcacc 
      ggaatgctg ccttggtcc gtacggaat atttttgtt 
      caggaaaac gttttcgaa acatttatc caaaaattg 
      aaacacaaa gttggtgaa ataagagtg ggacaaagc 
      gaccaaaaa aatttagat atttcgtca tcacttgat 
      aataaaacg ttgcaaaaa ttggtgaaa atcattgaa 
      agggcgaag gatgagggc gtggaagtg taccaacca 
      aacaataac gcaattccg actctattc atcggaggc 
      aacgttttc cacaataat gttatagtt gaggatgct 
      attaaagtc ccaattgta actttaaca gcttttcga 
      acgattaat gaagccgct gcattgtca aacaacact 
      aagcaaggc ctagcggct tcagtatgg acggaaagt 
      acgagttta gcaaacgaa ataatcgaa aaactcaag 
      gtcggaaca gtttggatc aattctcat ggtttattc 
      gcccccgat attcccttt tcaccgttc aaaagcagc 
      ggaaacgct ttcttcggc ggaactgaa ggattctac 
      gaatacgtt gatacaaaa aacttgaaa accgagaca 
      ccttcacaa gtgcgaaac gctaccagc atggaaaaa 
      gccataaat gaggcaaaa aaggcccaa aacgcctgg 
      ttcaaactt gaccacttt gaacgaacg aaaattctt 
      caaacagtg gctcacgaa atccaaaac aaagtatgg 
      ggtttaccc gacaattgg ataaataat tgggtcacc 
      ctgatccac gattgcctt tcaaatcac tcgttaaat 
      attgttacg aaaaaacaa agcttcaat gttgtaaca 
      atgcgagaa ccttgcgga cttatcgcg attgaaatt 
      tccgacgat aaagaaacg cataacaaa aaaatgata 
      attgcggct ttaattgaa ggcaattct gttattatt 
      ctcaatggt gccaaagac acgagtgat ttttacaca 
      ttcgttgcg aaactgctc ccaaagggc gttctaacg 
      ctcgttgaa cattcacaa gaggctacg aaaattgca 
      gcgttacat aaggagctg aatgtttat tttggggaa 
      aggaacaaa gtttttggc tccttgccg ctaaaaacc 
      tcaagtaaa tttaaagtg gtgcaaacg gacgactgg 
      aattgcgtc cgcaacaaa gtcactctg gttaaaagt 
      gtttggttc aataaggga acatctgtt tga
    mRNA sequence
    • >TC001327 RA
      gtggtgtcg cgttttgat aacagtgct tgataatga 
      taaggttct attagtgtt ttgaccgct ttcaacttt 
      taatcgcgt aaaatgggc gatattggg gaccctaag 
      cattccgta attaaacaa atatttgaa acaatgagt 
      tatggagac gatttcgaa aacctgact gaagcgaca 
      gagtggctc aagaccaaa gaaaatcgc atatttctc 
      catattgat aaaaaagat gaaacacca aatggagaa 
      cccttatca attaaaaat atcaacaac aaagagagt 
      ttatgtgag atttatgtc cctgaagca gctacaatt 
      gacgcagtt ttgaaaaag ttagggggg gctaaaaat 
      gcatgggct gaggtcgcc cccttgaaa cgagcagaa 
      atcattcac aagtacgta ttttgaaca cgtgggaac 
      gcaaagcga cgtttattt gttagattc gggggcgag 
      atttgcaag aaacaggat ttattcaca cagcttgaa 
      gttgcagtt cgtggcatt ctcagtaaa gacactaga 
      aaaagctta ccgttactt accgactgg ttgcaatac 
      tattccagc ttcagtgta aagtgtaat tcgaaaaat 
      tcttcatgg aaacccgag ggtgcgtac aatgagcgg 
      tttttccag acacgtttt tagacaaca tatgatata 
      acctcacct tcaagtgtc acaattttt gactgtttt 
      ttgtgtttg gacacaggg ccctattaa aaaggcgtt 
      ttaatttga ttttcaagt gataaaatg cgtgattta 
      agtttaaat gtctcaatt tttcgatat ttagtgttt 
      agtaacttc gatatttta atggcggta cgtctgatt 
      cgtctttta ggtttttat ttacgcgcg gatttaaat 
      tttttttag gcttagccg tggcaattt tatcagatg 
      tgaactcgc tagcgacct ttggctaca tactggggc 
      cttgtttag ccgcaggtt acaatgtag tgctccaga 
      ctggtccgc aactttctc tagttgcgt cccttttga 
      ttgagattg caattcaag cggggttgg ttatcacat 
      tttaatttt tttactaga gtcacaaca aatcttgcc 
      acgttttta gaataccag acgacacta ttcgattaa 
      taccggggg atgtggact ttaatatat ttctaatcc 
      acccccaag tgtccgtaa tttcaattt ttgatgatc 
      tttacaaag aaaaatatt taatagcaa accatttta 
      ataagaaat tgatcaatt tttattcgt cgggcattc 
      caatgataa tttttgaaa actgtgact tggattcag 
      cgtgtcatg cggtggtca atgcagttt gggggttca 
      ccggaatgc tgccttggt ccgtacgga atatttttg 
      ttcaggaaa acgttttcg aaacattta tccaaaaat 
      tgaaacaca aagttggtg aaataagag tgggacaaa 
      gcgaccaaa aaaatttag atatttcgt catcacttg 
      ataataaaa cgttgcaaa aattggtga aaatcattg 
      aaagggcga aggatgagg gcgtggaag tgtaccaac 
      caaacaata acgcaattc cgactctat tcatcggag 
      gcaacgttt tccacaata atgttatag ttgaggatg 
      ctattaaag tcccaattg taactttaa cagcttttc 
      gaacgatta atgaagccg ctgcattgt caaacaaca 
      ctaagcaag gcctagcgg cttcagtat ggacggaaa 
      gtacgagtt tagcaaacg aaataatcg aaaaactca 
      aggtcggaa cagtttgga tcaattctc atggtttat 
      tcgcccccg atattccct tttcaccgt tcaaaagca 
      gcggaaacg ctttcttcg gcggaactg aaggattct 
      acgaatacg ttgatacaa aaaacttga aaaccgaga 
      caccttcac aagtgcgaa acgctacca gcatggaaa 
      aagccataa atgaggcaa aaaaggccc aaaacgcct 
      ggttcaaac ttgaccact ttgaacgaa cgaaaattc 
      ttcaaacag tggctcacg aaatccaaa acaaagtat 
      ggggtttac ccgacaatt ggataaata attgggtca 
      ccctgatcc acgattgcc tttcaaatc actcgttaa 
      atattgtta cgaaaaaac aaagcttca atgttgtaa 
      caatgcgag aaccttgcg gacttatcg cgattgaaa 
      tttccgacg ataaagaaa cgcataaca aaaaaatga 
      taattgcgg ctttaattg aaggcaatt ctgttatta 
      ttctcaatg gtgccaaag acacgagtg atttttaca 
      cattcgttg cgaaactgc tcccaaagg gcgttctaa 
      cgctcgttg aacattcac aagaggcta cgaaaattg 
      cagcgttac ataaggagc tgaatgttt attttgggg 
      aaaggaaca aagtttttg gctccttgc cgctaaaaa 
      cctcaagta aatttaaag tggtgcaaa cggacgact 
      ggaattgcg tccgcaaca aagtcactc tggttaaaa 
      gtgtttggt tcaataagg gaacatctg tttgaaaat 
      ttgaaaaaa taaactact gttgcagat
    • >TC001327 RB
      gtggtgtcg cgttttgat aacagtgct tgataatga 
      taaggttct attagtgtt ttgaccgct ttcaacttt 
      taatcgcgt aaaatgggc gatattggg gaccctaag 
      cattccgta attaaacaa atatttgaa acaatgagt 
      tatggagac gatttcgaa aacctgact gaagcgaca 
      gagtggctc aagaccaaa gaaaatcgc atatttctc 
      catattgat aaaaaagat gaaacacca aatggagaa 
      cccttatca attaaaaat atcaacaac aaagagagt 
      ttatgtgag atttatgtc cctgaagca gctacaatt 
      gacgcagtt ttgaaaaag ttagggggg gctaaaaat 
      gcatgggct gaggtcgcc cccttgaaa cgagcagaa 
      atcattcac aaattcggg ggcgagatt tgcaagaaa 
      caggattta ttcacacag cttgaagtt gcagttcgt 
      ggcattctc agtaaagac actagaaaa agcttaccg 
      ttacttacc gactggttg caatactat tccagcttc 
      agtgtaaag tgtaattcg aaaaattct tcatggaaa 
      cccgagggc ttagccgtg gcaatttta tcagatgtg 
      aactcgcta gcgaccttt ggctacata ctggggcct 
      tgtttagcc gcaggttac aatgtagtg ctccagact 
      ggtccgcaa ctttctcta gttgcgtcc cttttgatt 
      gagattgca attcaagcg ggaatacca gacgacact 
      attcgatta ataccgggg gatgtggac tttaatata 
      tttctaatc cacccccaa gtgtccgta atttcaatt 
      tttgatgat ctttacaaa gaaaaatat ttaatagca 
      aaccatttt aataagaaa ttgatcaat ttttattcg 
      tcgggcatt ccaatgata atttttgaa aactgtgac 
      ttggattca gcgtgtcat gcggtggtc aatgcagtt 
      tgggggttc accggaatg ctgccttgg tccgtacgg 
      aatattttt gttcaggaa aacgttttc gaaacattt 
      atccaaaaa ttgaaacac aaagttggt gaaataaga 
      gtgggacaa agcgaccaa aaaaattta gatatttcg 
      tcatcactt gataataaa acgttgcaa aaattggtg 
      aaaatcatt gaaagggcg aaggatgag ggcgtggaa 
      gtgtaccaa ccaaacaat aacgcaatt ccgactcta 
      ttcatcgga ggcaacgtt ttccacaat aatgttata 
      gttgaggat gctattaaa gtcccaatt gtaacttta 
      acagctttt cgaacgatt aatgaagcc gctgcattg 
      tcaaacaac actaagcaa ggcctagcg gcttcagta 
      tggacggaa agtacgagt ttagcaaac gaaataatc 
      gaaaaactc aaggtcgga acagtttgg atcaattct 
      catggttta ttcgccccc gatattccc ttttcaccg 
      ttcaaaagc agcggaaac gctttcttc ggcggaact 
      gaaggattc tacgaatac gttgataca aaaaacttg 
      aaaaccgag acaccttca caagtgcga aacgctacc 
      agcatggaa aaagccata aatgaggca aaaaaggcc 
      caaaacgcc tggttcaaa cttgaccac tttgaacga 
      acgaaaatt cttcaaaca gtggctcac gaaatccaa 
      aacaaagta tggggttta cccgacaat tggataaat 
      aattgggtc accctgatc cacgattgc ctttcaaat 
      cactcgtta aatattgtt acgaaaaaa caaagcttc 
      aatgttgta acaatgcga gaaccttgc ggacttatc 
      gcgattgaa atttccgac gataaagaa acgcataac 
      aaaaaaatg ataattgcg gctttaatt gaaggcaat 
      tctgttatt attctcaat ggtgccaaa gacacgagt 
      gatttttac acattcgtt gcgaaactg ctcccaaag 
      ggcgttcta acgctcgtt gaacattca caagaggct 
      acgaaaatt gcagcgtta cataaggag ctgaatgtt 
      tattttggg gaaaggaac aaagttttt ggctccttg 
      ccgctaaaa acctcaagt aaatttaaa gtggtgcaa 
      acggacgac tggaattgc gtccgcaac aaagtcact 
      ctggttaaa agtgtttgg ttcaataag ggaacatct 
      gtttgaaaa tttgaaaaa ataaactac tgttgcaga 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY