Status
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Gene information
General information
TC001321 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14462193..14468009
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001321
    iBeetle screen iB_09701

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001321 RA
      MLNLEQEVG VWDSVLLEP LSEDTFIAN LQQRFKRDH 
      IYTYIGNVL VSVNPYKKL ALYSTDLVE TYVKRGPFQ 
      LPPHIYAIA GSAYRWLND RNEDQCIVV TGESGSGKT 
      EAARIVLQF LVLVSGDIV GVKLMKDRL VQANTLLEA 
      FGNARTMKN DNASRFGKF LDIEFDYKG DPVGGHLTH 
      YFLDKARVT SLSPGDRNF HIFYQLLAG ADIHLLKCL 
      KLQRNVEHY NILQTVNNQ PTLTDDEDK KLFVYTKSA 
      LEVLGFSVE DIIDIFQVI AIVLKLGNL QFVPCNNID 
      GTEGCAISN EYELFEICE LLGAEHRWL QRALTSRQI 
      EDGILADLN AYEASKIRD ILCKTLYSR LFTWLINKI 
      NEIIKGQRL GKRKVLGVL DLYGFEVFE KNSFEQLII 
      NYCNEKLHQ FITNVTLKE EQEEIIREG LEWAKIEYF 
      NNIVICQLL EHESHGILS LLEESHVQS DSCYLNRVE 
      QCCAGHSYC LAADATLPQ YSFQIRHYA GTVTYNVHE 
      FLEKNRDSL PKEISRAMF RFDHPLMKS LFPEGNPKR 
      CTFKRPVSK SVQLRVSLN SLLKSLNSR QIHYIRCLK 
      PNEMKQPRI LEMALIQHQ VRYLGLVPT VQIWRSGYC 
      YRLAYPQFL CRYKMICGN TWPRWRGSP IEGVSVLLR 
      SLPIPSAEF TFGRNKLFV RSPRTVFEL EDFRRVRLH 
      DLALLIQKS WRGFSQKRK YQKLRRCQM VIASAWRSW 
      RAQEEYRTL KHRKQVEWA ARVIQRHYI QWKRRRFLQ 
      TFCKLLPPD ADSPICREW PSCSSRLAE CNNLLKKIH 
      HRWRCGKYR LKFDQTARN RMREKVTAS FIFKDRKCS 
      YPRSVSHPF LGDYVRLRQ NIQWKKICL ESNDQYVVF 
      ADIINKIAR SSGKFVPIL LVVSTRSML ILDQRTLQI 
      KYRVPATEI YRMSLSPFL DDVAVVHVR ASSIANADS 
      ASSNSDATG CLFQSDLSK KKGDFVFQT GHVIEVVTK 
      LFLVVQNAT GKPPEVNIT TEFEANFGS QTVTFTFKC 
      MGLPEVQPG QIRVLRKGN KMEVHV
    CDS sequence
    • >TC001321 RA
      atgctcaac ttggagcag gaagtggga gtttgggat 
      tctgtactt cttgagccc ttgtcggaa gataccttc 
      attgctaat ttacaacaa cggttcaag agagaccat 
      atctacaca tacattggg aacgtgctt gtctccgtt 
      aacccctac aaaaaacta gctttatat tcgactgat 
      cttgtagaa acgtacgtc aaaaggggc ccattccag 
      cttccccct cacatatat gcgattgct gggtccgct 
      tacaggtgg ctaaatgac cgaaatgag gaccagtgc 
      attgttgtc acaggcgaa agtggctca ggaaaaacg 
      gaagctgca agaattgtt ttacagttt ttagtttta 
      gtttcgggc gatattgtt ggagttaaa cttatgaaa 
      gaccgccta gttcaagct aacacttta ctggaagct 
      ttcggaaat gctagaacg atgaaaaat gataacgcg 
      tccagattt ggtaaattt ttggacatt gagtttgac 
      tacaagggg gacccggtc gggggccac ttgacccac 
      tattttttg gataaggcc agagttacg agtttatcg 
      ccaggtgat cgcaatttt catattttt tatcaatta 
      cttgctggt gctgatatt catctttta aaatgccta 
      aaacttcaa cgcaatgtc gagcattac aacatcttg 
      caaactgta aataaccaa ccgacctta accgacgac 
      gaagataaa aagttgttc gtttacacg aaaagtgca 
      ctggaggtg ttgggtttt agcgttgaa gacattatc 
      gatatattc caagttatt gcaattgtg ttaaaatta 
      ggcaatttg caatttgtg ccttgtaat aacatagat 
      ggtaccgaa ggttgcgca atcagtaac gaatacgaa 
      ttgttcgaa atttgcgaa ttgttgggc gctgagcac 
      cgatggctt cagagagcc ttaacgagt cggcaaatt 
      gaagacgga attttagct gacctcaac gcgtacgaa 
      gcgtcaaaa atcagggat attttatgc aaaactttg 
      tacagccgg ctttttaca tggctgatt aacaaaata 
      aacgaaata attaagggc caaaggctg ggcaaacgt 
      aaggttttg ggcgtgttg gacttgtac gggtttgaa 
      gtgtttgag aaaaactca ttcgagcag cttatcatc 
      aactactgc aacgagaag ttgcatcag ttcattacg 
      aatgttacg ctgaaggag gagcaagaa gagataatc 
      agggagggg ctcgagtgg gcaaaaata gaatatttt 
      aacaacata gttatttgt caattgttg gagcatgaa 
      agtcacggc attttgtca ctgttggag gaatcgcac 
      gttcagagc gactcttgc tatttgaat agagtcgaa 
      cagtgttgt gctgggcat tcgtattgt ctagcagcc 
      gatgccaca ctgccacaa tatagtttt caaattcgt 
      cattatgcg ggtactgtc acttacaac gtgcacgaa 
      tttctggaa aaaaatcga gattcgttg cctaaagaa 
      atatctcga gctatgttt cgtttcgat caccctcta 
      atgaaatcg ctttttccc gaaggaaac cccaaacgt 
      tgcactttc aaacgacca gtctctaag tcagtccaa 
      ttgcgggtg tctttaaac tcgcttttg aaaagttta 
      aactcgcgt cagattcac tacatccgg tgcttgaaa 
      ccgaacgaa atgaaacaa ccccgcatt ttagaaatg 
      gccctcatc caacaccag gtgcgttat ctggggttg 
      gtcccaacg gtgcaaatc tggcgctcg ggctattgc 
      tatcgcttg gcctatccg cagtttctt tgccggtat 
      aagatgatt tgtgggaac acctggccc agatggcgc 
      gggtctccc atagaaggt gtttcagtg cttttacga 
      tcactgcca attccaagt gcggaattt accttcggt 
      cgaaataaa ttgttcgtg aggagtcca cgaactgtt 
      tttgaattg gaggatttt agaagggtg aggttgcac 
      gatttggcg cttttgata cagaaatcc tggagaggg 
      ttcagtcag aagaggaag taccagaag ttgaggagg 
      tgtcagatg gtcattgct tcggcctgg aggagttgg 
      cgggcacag gaagagtac aggacgtta aaacaccgc 
      aaacaagtc gaatgggcc gctagagta attcaaaga 
      cattatatt caatggaag cgaagacga tttcttcaa 
      acattttgt aaactattg ccgcccgat gccgactct 
      cctatttgt agagaatgg ccatcttgt tcttccaga 
      ttagctgaa tgtaataat ctattgaaa aaaatccac 
      catcgttgg cgctgtggt aaataccgc cttaagttc 
      gaccaaact gcgcgaaat cgcatgagg gaaaaagta 
      accgccagt tttattttc aaagacagg aagtgttca 
      tatcctaga agcgtttcg catccattt cttggggat 
      tacgttcgg ctaaggcaa aacattcaa tggaaaaaa 
      atctgttta gagagcaac gaccaatat gtagttttc 
      gctgacatt attaataaa atagcgcgt tctagtggc 
      aagttcgtg ccaatcctc ctggttgtt tccacacga 
      tcgatgttg attttggat caacggacg ttgcagata 
      aaatatcgc gtgcctgcg actgaaatt tacaggatg 
      tctctgtcg ccgttcctc gacgacgtg gccgtggtc 
      cacgtgcgt gcgtcgtcg atagccaac gcagacagc 
      gctagttcg aattcggac gcaactggt tgcttattt 
      cagagcgat ttgagtaag aaaaagggc gatttcgtg 
      ttccaaacg gggcacgtg atcgaagtc gtaacgaaa 
      ttgttcctg gtcgtgcaa aacgccaca gggaagccg 
      cccgaagtc aacataacg acagagttt gaggcgaat 
      ttcgggtcg caaacggtg actttcacc ttcaaatgc 
      atgggcttg cctgaagtg caacccggg cagatccgc 
      gtgcttcgc aaaggcaac aaaatggaa gttcatgtg 
      tga
    mRNA sequence
    • >TC001321 RA
      tggcacaac ccgacctgt caagtgaaa agttttatt 
      ggataattt ttaacttaa ttaaaatgg gacggctgt 
      tctaattac ggcgataat tataaaatg gaccgaacc 
      gttttttcc accagttac atcccagtc atttaaaat 
      ttgctattt gaaattagt ttgaatttg gggcacagt 
      gaaaagaag tggaaggac tagatgccg cgctggcgg 
      cagtacttc aaggggcgg tgcgatgct caacttgga 
      gcaggaagt gggagtttg ggattctgt acttcttga 
      gcccttgtc ggaagatac cttcattgc taatttaca 
      acaacggtt caagagaga ccatatcta cacatacat 
      tgggaacgt gcttgtctc cgttaaccc ctacaaaaa 
      actagcttt atattcgac tgatcttgt agaaacgta 
      cgtcaaaag gggcccatt ccagcttcc ccctcacat 
      atatgcgat tgctgggtc cgcttacag gtggctaaa 
      tgaccgaaa tgaggacca gtgcattgt tgtcacagg 
      cgaaagtgg ctcaggaaa aacggaagc tgcaagaat 
      tgttttaca gtttttagt tttagtttc gggcgatat 
      tgttggagt taaacttat gaaagaccg cctagttca 
      agctaacac tttactgga agctttcgg aaatgctag 
      aacgatgaa aaatgataa cgcgtccag atttggtaa 
      atttttgga cattgagtt tgactacaa gggggaccc 
      ggtcggggg ccacttgac ccactattt tttggataa 
      ggccagagt tacgagttt atcgccagg tgatcgcaa 
      ttttcatat tttttatca attacttgc tggtgctga 
      tattcatct tttaaaatg cctaaaact tcaacgcaa 
      tgtcgagca ttacaacat cttgcaaac tgtaaataa 
      ccaaccgac cttaaccga cgacgaaga taaaaagtt 
      gttcgttta cacgaaaag tgcactgga ggtgttggg 
      ttttagcgt tgaagacat tatcgatat attccaagt 
      tattgcaat tgtgttaaa attaggcaa tttgcaatt 
      tgtgccttg taataacat agatggtac cgaaggttg 
      cgcaatcag taacgaata cgaattgtt cgaaatttg 
      cgaattgtt gggcgctga gcaccgatg gcttcagag 
      agccttaac gagtcggca aattgaaga cggaatttt 
      agctgacct caacgcgta cgaagcgtc aaaaatcag 
      ggatatttt atgcaaaac tttgtacag ccggctttt 
      tacatggct gattaacaa aataaacga aataattaa 
      gggccaaag gctgggcaa acgtaaggt tttgggcgt 
      gttggactt gtacgggtt tgaagtgtt tgagaaaaa 
      ctcattcga gcagcttat catcaacta ctgcaacga 
      gaagttgca tcagttcat tacgaatgt tacgctgaa 
      ggaggagca agaagagat aatcaggga ggggctcga 
      gtgggcaaa aatagaata ttttaacaa catagttat 
      ttgtcaatt gttggagca tgaaagtca cggcatttt 
      gtcactgtt ggaggaatc gcacgttca gagcgactc 
      ttgctattt gaatagagt cgaacagtg ttgtgctgg 
      gcattcgta ttgtctagc agccgatgc cacactgcc 
      acaatatag ttttcaaat tcgtcatta tgcgggtac 
      tgtcactta caacgtgca cgaatttct ggaaaaaaa 
      tcgagattc gttgcctaa agaaatatc tcgagctat 
      gtttcgttt cgatcaccc tctaatgaa atcgctttt 
      tcccgaagg aaaccccaa acgttgcac tttcaaacg 
      accagtctc taagtcagt ccaattgcg ggtgtcttt 
      aaactcgct tttgaaaag tttaaactc gcgtcagat 
      tcactacat ccggtgctt gaaaccgaa cgaaatgaa 
      acaaccccg cattttaga aatggccct catccaaca 
      ccaggtgcg ttatctggg gttggtccc aacggtgca 
      aatctggcg ctcgggcta ttgctatcg cttggccta 
      tccgcagtt tctttgccg gtataagat gatttgtgg 
      gaacacctg gcccagatg gcgcgggtc tcccataga 
      aggtgtttc agtgctttt acgatcact gccaattcc 
      aagtgcgga atttacctt cggtcgaaa taaattgtt 
      cgtgaggag tccacgaac tgtttttga attggagga 
      ttttagaag ggtgaggtt gcacgattt ggcgctttt 
      gatacagaa atcctggag agggttcag tcagaagag 
      gaagtacca gaagttgag gaggtgtca gatggtcat 
      tgcttcggc ctggaggag ttggcgggc acaggaaga 
      gtacaggac gttaaaaca ccgcaaaca agtcgaatg 
      ggccgctag agtaattca aagacatta tattcaatg 
      gaagcgaag acgatttct tcaaacatt ttgtaaact 
      attgccgcc cgatgccga ctctcctat ttgtagaga 
      atggccatc ttgttcttc cagattagc tgaatgtaa 
      taatctatt gaaaaaaat ccaccatcg ttggcgctg 
      tggtaaata ccgccttaa gttcgacca aactgcgcg 
      aaatcgcat gagggaaaa agtaaccgc cagttttat 
      tttcaaaga caggaagtg ttcatatcc tagaagcgt 
      ttcgcatcc atttcttgg ggattacgt tcggctaag 
      gcaaaacat tcaatggaa aaaaatctg tttagagag 
      caacgacca atatgtagt tttcgctga cattattaa 
      taaaatagc gcgttctag tggcaagtt cgtgccaat 
      cctcctggt tgtttccac acgatcgat gttgatttt 
      ggatcaacg gacgttgca gataaaata tcgcgtgcc 
      tgcgactga aatttacag gatgtctct gtcgccgtt 
      cctcgacga cgtggccgt ggtccacgt gcgtgcgtc 
      gtcgatagc caacgcaga cagcgctag ttcgaattc 
      ggacgcaac tggttgctt atttcagag cgatttgag 
      taagaaaaa gggcgattt cgtgttcca aacggggca 
      cgtgatcga agtcgtaac gaaattgtt cctggtcgt 
      gcaaaacgc cacagggaa gccgcccga agtcaacat 
      aacgacaga gtttgaggc gaatttcgg gtcgcaaac 
      ggtgacttt caccttcaa atgcatggg cttgcctga 
      agtgcaacc cgggcagat ccgcgtgct tcgcaaagg 
      caacaaaat ggaagttca tgtgtgaca ttttgccaa 
      acatatcct cgagaccag cgtattgat cactgccat 
      tagatttgc acaatagat tgggagaaa tgttccgtt 
      gaccaaaaa ttgatccta gcttagcgt gagagtttt 
      aaatttgtg ttatggctg aaattttaa agtaccttt 
      acattgaac aatcacgga cgaactagc ttacgtatt 
      cataaaccc cattaagat gcaaggtat aaggatagt 
      tcgacaatt cttgttaat aaattacgt ctcgtata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY