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Gene information
General information
TC000248 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14191742..14194641
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000248
    iBeetle screen iB_09699

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000248 RA
      MGGFGAQEK CGEGSKVHF KCVPYHRCD GNTNTIIPE 
      EEDTDTNDY SLLIDIRLG VPKLCSKAL DVCCQIPNT 
      TSEIPPTTK PQPTDNTPK PQPTPNLSF CGIRNNNGI 
      DFKITGNSH NEAEYGEFP WMVAVLKKN YDPSVDQGF 
      ALCGGSLIA PRVILTGAH CVHKFKPEE IKIRAGEWD 
      TQTEDERFP YQERQVEEI IIHEAFKDG TLLNDVALI 
      VLEKPFLKA KHIGTVCLP NQDQVISSQ ECFGTGWGK 
      NAYGSEGQY QMILKKIEL PIVPHAQCE TALRKTKLG 
      NFFKLHKSF TCAGGIPGR DTCTGDGGS PLVCPDPEN 
      PARYIQAGI VAWGVGCGT TTPGVYADV AKFRGWVDA 
      QMERLELSN KPYTT
    CDS sequence
    • >TC000248 RA
      atgggcggg tttggggca caggagaaa tgtggcgaa 
      ggcagtaaa gttcatttt aaatgcgtg ccgtaccat 
      cgctgtgac ggtaacacg aataccatt ataccggag 
      gaggaagac acggatact aatgattat agtttatta 
      attgatatc aggctaggt gtacccaaa ctatgctcc 
      aaagctctg gacgtgtgt tgtcaaata ccaaatacc 
      acatctgaa attccaccc acaacaaaa ccacaacca 
      actgacaac accccaaaa ccacaacca acccccaac 
      ttaagcttc tgtggtatt cgcaataac aacggcatt 
      gattttaaa atcacgggc aattcgcac aacgaagcc 
      gaatatggc gaatttccc tggatggta gcagttttg 
      aaaaaaaat tacgaccca tctgtcgat caaggattc 
      gccttgtgt ggaggctca ctcatcgcg cctcgagtt 
      attttaacc ggagctcat tgtgtccac aagtttaaa 
      cctgaagag attaaaata cgggcgggg gaatgggac 
      actcagact gaagacgag cgattcccg tatcaggaa 
      agacaagtc gaggaaata ataattcat gaagctttc 
      aaagacggg acgcttctg aacgacgtc gctttgata 
      gttttggaa aaaccgttt cttaaagcc aaacacatc 
      ggaacggtt tgtttgcca aaccaggac caagttatt 
      agctcgcag gagtgtttc gggacgggt tgggggaaa 
      aacgcttat gggagcgaa ggccagtac caaatgatt 
      ttgaaaaaa atagagctc ccaattgtg ccacacgcg 
      cagtgcgaa actgcactc aggaaaacc aaattgggg 
      aatttcttc aagttgcac aagagtttt acttgcgct 
      gggggaatt cccgggagg gacacttgc actggagac 
      gggggaagt cccttggtt tgcccagac cccgaaaac 
      ccagcgcgc tacatccaa gccggaatc gtagcctgg 
      ggcgtgggt tgcgggaca acaactccg ggggtttac 
      gctgatgtt gcaaaattc cggggttgg gtggacgcg 
      caaatggaa agactggag cttagtaat aagccttac 
      acgacctag
    mRNA sequence
    • >TC000248 RA
      tacctgtga gcaaagaat ttttaagtg agtttggct 
      gctgtgttg taacctgtt gttatataa ttgactcaa 
      ggtgaccgt ggaatcagt tacagtact ctttgagaa 
      tgttgagtg ttattttac tgacggttt ggtgcggac 
      ttgtttgga cgcaaactg attttgttc ctacaaatt 
      ttgaggagg ttactaagc ccactatgg gcgggtttg 
      gggcacagg agaaatgtg gcgaaggca gtaaagttc 
      attttaaat gcgtgccgt accatcgct gtgacggta 
      acacgaata ccattatac cggaggagg aagacacgg 
      atactaatg attatagtt tattaattg atatcaggc 
      taggtgtac ccaaactat gctccaaag ctctggacg 
      tgtgttgtc aaataccaa ataccacat ctgaaattc 
      cacccacaa caaaaccac aaccaactg acaacaccc 
      caaaaccac aaccaaccc ccaacttaa gcttctgtg 
      gtattcgca ataacaacg gcattgatt ttaaaatca 
      cgggcaatt cgcacaacg aagccgaat atggcgaat 
      ttccctgga tggtagcag ttttgaaaa aaaattacg 
      acccatctg tcgatcaag gattcgcct tgtgtggag 
      gctcactca tcgcgcctc gagttattt taaccggag 
      ctcattgtg tccacaagt ttaaacctg aagagatta 
      aaatacggg cgggggaat gggacactc agactgaag 
      acgagcgat tcccgtatc aggaaagac aagtcgagg 
      aaataataa ttcatgaag ctttcaaag acgggacgc 
      ttctgaacg acgtcgctt tgatagttt tggaaaaac 
      cgtttctta aagccaaac acatcggaa cggtttgtt 
      tgccaaacc aggaccaag ttattagct cgcaggagt 
      gtttcggga cgggttggg ggaaaaacg cttatggga 
      gcgaaggcc agtaccaaa tgattttga aaaaaatag 
      agctcccaa ttgtgccac acgcgcagt gcgaaactg 
      cactcagga aaaccaaat tggggaatt tcttcaagt 
      tgcacaaga gttttactt gcgctgggg gaattcccg 
      ggagggaca cttgcactg gagacgggg gaagtccct 
      tggtttgcc cagaccccg aaaacccag cgcgctaca 
      tccaagccg gaatcgtag cctggggcg tgggttgcg 
      ggacaacaa ctccggggg tttacgctg atgttgcaa 
      aattccggg gttgggtgg acgcgcaaa tggaaagac 
      tggagctta gtaataagc cttacacga cctagttgt 
      acatacgat aacttttga tctcgaaat aaacacaag 
      gcgataat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY