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Gene information
General information
TC032250
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000252
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14127510..14168263
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032250
    iBeetle screen iB_09698

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032250 RA
      MDNADIQLV GGPCGQHGP YTFYKAFKY TKNGVKRIV 
      TLSEFFFVK LWIDSDLLC IGELQLLWS DKNSDQVLA 
      SLRLYFLPE NTPEGRMDH GEDEVLAIS EKVVLRVED 
      LITWITVDA EWTWGRLAK CEKDVKDPP KSEDNKNTI 
      GSLTESSLD FSDVEKERK ASDCNSEHS GPPVVILSY 
      PRYCRYRAM MRRLEGVEN EYLKYKIVN ALGGFTAYT 
      KNTRVLFCK DTFDYPELE GHELLCNHL APKLKGRPR 
      GKRKKRSVS PGSESNESE SSVSNVFNS SKENGATDG 
      GKNGLRCDN THLRRSTRA TESNESKDF IKELTVFMK 
      TNYTPIGKI PSLGYKELD LYSFYTKVQ KLGGYDSVT 
      ANRLWKSIF DDMSGHASS TSAATVIRR HYERFLLPY 
      ERHVKGEEY KPLPISERR RLKSKTGSS TSDAETSES 
      TSRSGNSTP VPVTPTTPT ENKEFNLQG KTSSLRSVR 
      VKPERQNKQ ISDKLNIQN NNNEVKTED ESEVKCEVV 
      AVKAEETVP EKDAEPLTS AVAPIAQKL TPEKSHSPV 
      EGKENIPIK TGDTPAKSP EVIEVPYKP KTPEIIDLE 
      ADTYPKTTL HQPFLHEVK KRKLEILKE GGLEVTPVR 
      SFAPPTSTK EIRPSVIQT AGTQVVHRP IKNEMPPPP 
      PKRPQIAIP QALSISHVK LDQKQPPPK PFPYVNGNV 
      PPKVLQSKS IYSYSEKTV YGNPKDLMP STQPVHTPK 
      FVGTRTGGD ILDLTVTSP QKPIVEIMK IPTPTPQPP 
      TFTSPSKVP YKPPLIDGR RLGSNLEIT LVGSNKPSV 
      HQSSKYLPP PSLTNYNMS RYHPQKRVS SDHYTNHKI 
      PRIDENGKY GKLPPAPVL STRENKSLE LSVPQPYGS 
      RNHQAPIKS EAKVPPQMP PVSNAKHVP GPAFPYLSH 
      LYEGNKGVA PYVPFLDPI YYTAAMQSL YSHSPLTAT 
      HPMPPFPST TEHLKLYSD LLAHNSRLN FPFPIGSEG 
      NPSILNNNN SLKKP
    CDS sequence
    • >TC032250 RA
      atggataat gccgacata cagctggtg ggggggccg 
      tgcggccaa cacgggccg tatacgttc tacaaggcg 
      tttaaatac acgaaaaac ggtgtcaaa agaatagtg 
      actttgagt gaatttttc tttgttaag ctgtggatt 
      gactcggat ttgttgtgc attggtgaa ttgcagttg 
      ttatggagt gataaaaat agtgaccaa gtgctggcc 
      agtttacgg ctttacttc cttccagag aatacgccg 
      gaaggtcgc atggaccac ggagaggac gaagtgctg 
      gcaatctcc gaaaaagtc gtcctgcga gtggaagat 
      ttaataact tggatcaca gtagatgca gaatggacg 
      tggggtaga ttagccaag tgcgaaaag gacgtaaaa 
      gatccgcca aaaagcgag gacaacaaa aacaccatc 
      ggatcgctt actgaaagc tccttggac ttttcggac 
      gtggaaaag gaaagaaaa gcttcagat tgcaactcg 
      gagcactcg ggaccgccg gtggtgatc ctgagctat 
      ccgcgctac tgcagatac cgtgcaatg atgagaaga 
      ttagaaggt gtcgagaac gaatacctc aaatacaaa 
      attgtaaac gctttgggc ggcttcaca gcctacact 
      aaaaacaca cgcgtcctg ttttgcaag gacactttc 
      gattatccc gaattagaa ggtcacgaa ttactttgc 
      aaccacctc gctcccaaa ctcaaaggc agacccaga 
      ggcaagcgg aaaaaacga agtgtctct ccgggttca 
      gagagcaac gaatcagag tcttcagta tcaaatgtg 
      ttcaattcg tctaaagag aatggtgct actgacggt 
      ggcaaaaac gggctacgg tgcgacaac actcacctc 
      agacgcagc acaagagct acggaaagt aacgaaagc 
      aaagatttc atcaaagaa ttgactgtt ttcatgaaa 
      actaactat acgcctata ggaaaaata ccctcgtta 
      ggatacaaa gaactcgat ttgtattcg ttttacacc 
      aaggtgcaa aagttggga ggctacgat tcggtcaca 
      gcaaatcga ctctggaag tctattttt gatgatatg 
      agcgggcac gctagtagc acaagtgct gccaccgtc 
      atacgaaga cactacgaa aggtttttg ctgccgtat 
      gagcgccac gtgaaaggc gaagagtac aagccgttg 
      ccaatttca gaaagacga agactgaaa agtaaaact 
      ggaagcagc accagcgat gctgaaaca agcgaaagc 
      acatcaagg agtggcaat agcactcca gttccggtt 
      actccaacc actccaact gaaaacaag gaatttaat 
      ctacaaggg aagacgtcg tccttgcgg agtgtgcgc 
      gttaaaccg gaaagacag aacaaacaa attagtgac 
      aagttgaac atacaaaac aataataac gaagttaaa 
      accgaagac gaatccgag gtgaaatgt gaggtagtg 
      gcggtcaaa gctgaagaa acagtgccc gaaaaggac 
      gccgaaccg ttgacttcg gcagtggcg ccaatcgca 
      caaaaactc acccctgaa aaatctcat tcgccagtc 
      gagggcaaa gaaaacatc cccatcaaa acgggggat 
      accccggcc aaaagtccc gaagtcatc gaggtgcca 
      tacaaaccc aaaaccccc gaaatcatc gaccttgaa 
      gcggacact taccccaaa accaccctc caccaacca 
      ttcctccac gaagtcaag aaacgcaaa ttagagatt 
      ttgaaggaa ggcggtctc gaagtgaca ccagttcgt 
      agtttcgct ccacccacg tcaacaaaa gagatccgg 
      ccgtcggtt atccaaaca gcaggaacg caagtagtc 
      cacagacca atcaaaaat gaaatgcca ccgccgccg 
      ccaaaacgg ccccaaatc gcgatccca caagccctt 
      agcataagt cacgttaaa ctcgatcaa aaacaacca 
      ccccccaaa ccattccca tacgttaac ggcaacgtc 
      ccccccaaa gtcctccaa tcgaaatca atctactca 
      tattcggaa aaaaccgtc tacggcaac cccaaagac 
      ctaatgccg agcacccaa ccagtccac acccccaag 
      ttcgtgggc acgcgaacc gggggcgac atcctcgac 
      ctgacggta accagcccc caaaaacca atagtcgaa 
      atcatgaaa ataccaacc cccacgcca caacccccc 
      acgttcacg tccccgtcg aaagttccg tacaaaccg 
      ccactgatc gacggccgc cggttaggc tcaaacctt 
      gaaataaca ctagttggg tcaaataaa ccaagcgtt 
      caccaatcc tcgaaatac ctaccgcca ccgtctctc 
      acaaactac aacatgagc agataccac ccccagaaa 
      cgtgtatcg tccgaccat tacaccaac cacaaaatt 
      ccccgaatc gacgaaaac ggaaagtat ggcaaactc 
      ccgccggct ccggttttg tcgactcgg gagaacaag 
      tcgttggaa ctgagcgtt ccgcagcct tatggctcc 
      aggaaccac caagcgcca atcaaaagc gaggccaaa 
      gtgccgccg cagatgccc ccggtgtcc aacgccaaa 
      catgtaccg ggtccggcg tttccgtat ttgtctcac 
      ttgtacgag ggtaataag ggggtggcg ccctatgtg 
      ccctttttg gacccgatt tattacacg gcagcgatg 
      caaagtctg tactcgcac agtccgctg actgcgacc 
      catccaatg ccgccgttc ccctcgaca acggaacat 
      ttaaagttg tattcggat ttgttggcg cacaattcg 
      cgcttgaat tttccgttt ccgattggg tcagaaggg 
      aatccttcc attcttaac aataacaac agtttgaag 
      aagccgtaa
    mRNA sequence
    • >TC032250 RA
      aataaaaaa ccatttatg taagaccga tttttttat 
      tttaatact tttagttac attcaactc ggagaatat 
      caattaaaa acaataaag gccggcatt gaatagttc 
      tataggatg agtcaacag aataacccc ctattaact 
      caacctgta taatgtaaa aatcaattc cctatcttc 
      ccaaatatc gactaatta cctattttt aatacaact 
      taaaaataa cacaggaag ttctacaaa aatataatt 
      aacataata atgactata cacattaca tgttttttt 
      taatttata ctgcagaaa cgttattgt ctttcaaat 
      atgcacctg cagaaatca ttaattatt tacacatta 
      atactcttt acttgctac aacctaaca taatgtgcc 
      aaacttccg tatattatt gtaatcatt acacttaca 
      acggaacta cactttcta aattactta taaacaatc 
      ttagtcaca aattatcta aactaaatt atacaaaca 
      tttttttaa gttttacta caaaagaca aacacttac 
      aacaaagaa tattttcca taagctaaa catttttta 
      aatgcaaaa taaacaaaa aaaactcca attacgtaa 
      ttcatctgc gtcagtgct aacatttta ttattcttc 
      tgtgaaaaa agaaaatgc ctgatttat gaaactgtt 
      aatttgctt ttataaata ggtgaaata aaaataaac 
      ggagtgcgt cgttcccca gcgcgaaaa ttcgaaatt 
      aataaacaa attaaaaat acttttgtt tatgtaccc 
      taaatctat acgacctca attaaataa aaaaaagtc 
      acacatgag agaaaatca aaaccgcga tactgaatc 
      atttttagg attgtgctc atgacgcac atgcaaata 
      tcacaatta tgcattttt ttataatta taccactgg 
      acaaagtat aaataaaga cccgttcac cttgaactt 
      catcggtta ggcaaccaa caatacgtc cggtcagct 
      gttcccgaa aacaatggc aaaaaccgg gaactctgg 
      acagcaccc tgcgatcgc cccctcgac gtgggaacg 
      tccctgcaa ccaggaaat taccacact cacacttac 
      ggctcactt tccctcgct ggaaccgct ttcgttgag 
      tgcaagcac ggcagtgtc atttctttg tgccccatt 
      cctccttcc accatttgt tgcagaagc gccagcatg 
      agcgaagcc tcgcggaag aagccgact ttttccgag 
      cgcagctct gtatcgtga cgtcatata attagagta 
      aatacaaag tagacgatg gtgtcagcg tgttgtttg 
      tagtgctct gtgattaat agtgaaaaa aaatggata 
      atgccgaca tacagctgg tgggggggc cgtgcggcc 
      aacacgggc cgtatacgt tctacaagg cgtttaaat 
      acacgaaaa acggtgtca aaagaatag tgactttga 
      gtgaatttt tctttgtta agctgtgga ttgactcgg 
      atttgttgt gcattggtg aattgcagt tgttatgga 
      gtgataaaa atagtgacc aagtgctgg ccagtttac 
      ggctttact tccttccag agaatacgc cggaaggtc 
      gcatggacc acggagagg acgaagtgc tggcaatct 
      ccgaaaaag tcgtcctgc gagtggaag atttaataa 
      cttggatca cagtagatg cagaatgga cgtggggta 
      gattagcca agtgcgaaa aggacgtaa aagatccgc 
      caaaaagcg aggacaaca aaaacacca tcggatcgc 
      ttactgaaa gctccttgg acttttcgg acgtggaaa 
      aggaaagaa aagcttcag attgcaact cggagcact 
      cgggaccgc cggtggtga tcctgagct atccgcgct 
      actgcagat accgtgcaa tgatgagaa gattagaag 
      gtgtcgaga acgaatacc tcaaataca aaattgtaa 
      acgctttgg gcggcttca cagcctaca ctaaaaaca 
      cacgcgtcc tgttttgca aggacactt tcgattatc 
      ccgaattag aaggtcacg aattacttt gcaaccacc 
      tcgctccca aactcaaag gcagaccca gaggcaagc 
      ggaaaaaac gaagtgtct ctccgggtt cagagagca 
      acgaatcag agtcttcag tatcaaatg tgttcaatt 
      cgtctaaag agaatggtg ctactgacg gtggcaaaa 
      acgggctac ggtgcgaca acactcacc tcagacgca 
      gcacaagag ctacggaaa gtaacgaaa gcaaagatt 
      tcatcaaag aattgactg ttttcatga aaactaact 
      atacgccta taggaaaaa taccctcgt taggataca 
      aagaactcg atttgtatt cgttttaca ccaaggtgc 
      aaaagttgg gaggctacg attcggtca cagcaaatc 
      gactctgga agtctattt ttgatgata tgagcgggc 
      acgctagta gcacaagtg ctgccaccg tcatacgaa 
      gacactacg aaaggtttt tgctgccgt atgagcgcc 
      acgtgaaag gcgaagagt acaagccgt tgccaattt 
      cagaaagac gaagactga aaagtaaaa ctggaagca 
      gcaccagcg atgctgaaa caagcgaaa gcacatcaa 
      ggagtggca atagcactc cagttccgg ttactccaa 
      ccactccaa ctgaaaaca aggaattta atctacaag 
      ggaagacgt cgtccttgc ggagtgtgc gcgttaaac 
      cggaaagac agaacaaac aaattagtg acaagttga 
      acatacaaa acaataata acgaagtta aaaccgaag 
      acgaatccg aggtgaaat gtgaggtag tggcggtca 
      aagctgaag aaacagtgc ccgaaaagg acgccgaac 
      cgttgactt cggcagtgg cgccaatcg cacaaaaac 
      tcacccctg aaaaatctc attcgccag tcgagggca 
      aagaaaaca tccccatca aaacggggg ataccccgg 
      ccaaaagtc ccgaagtca tcgaggtgc catacaaac 
      ccaaaaccc ccgaaatca tcgaccttg aagcggaca 
      cttacccca aaaccaccc tccaccaac cattcctcc 
      acgaagtca agaaacgca aattagaga ttttgaagg 
      aaggcggtc tcgaagtga caccagttc gtagtttcg 
      ctccaccca cgtcaacaa aagagatcc ggccgtcgg 
      ttatccaaa cagcaggaa cgcaagtag tccacagac 
      caatcaaaa atgaaatgc caccgccgc cgccaaaac 
      ggccccaaa tcgcgatcc cacaagccc ttagcataa 
      gtcacgtta aactcgatc aaaaacaac cacccccca 
      aaccattcc catacgtta acggcaacg tccccccca 
      aagtcctcc aatcgaaat caatctact catattcgg 
      aaaaaaccg tctacggca accccaaag acctaatgc 
      cgagcaccc aaccagtcc acaccccca agttcgtgg 
      gcacgcgaa ccgggggcg acatcctcg acctgacgg 
      taaccagcc cccaaaaac caatagtcg aaatcatga 
      aaataccaa cccccacgc cacaacccc ccacgttca 
      cgtccccgt cgaaagttc cgtacaaac cgccactga 
      tcgacggcc gccggttag gctcaaacc ttgaaataa 
      cactagttg ggtcaaata aaccaagcg ttcaccaat 
      cctcgaaat acctaccgc caccgtctc tcacaaact 
      acaacatga gcagatacc acccccaga aacgtgtat 
      cgtccgacc attacacca accacaaaa ttccccgaa 
      tcgacgaaa acggaaagt atggcaaac tcccgccgg 
      ctccggttt tgtcgactc gggagaaca agtcgttgg 
      aactgagcg ttccgcagc cttatggct ccaggaacc 
      accaagcgc caatcaaaa gcgaggcca aagtgccgc 
      cgcagatgc ccccggtgt ccaacgcca aacatgtac 
      cgggtccgg cgtttccgt atttgtctc acttgtacg 
      agggtaata agggggtgg cgccctatg tgccctttt 
      tggacccga tttattaca cggcagcga tgcaaagtc 
      tgtactcgc acagtccgc tgactgcga cccatccaa 
      tgccgccgt tcccctcga caacggaac atttaaagt 
      tgtattcgg atttgttgg cgcacaatt cgcgcttga 
      attttccgt ttccgattg ggtcagaag ggaatcctt 
      ccattctta acaataaca acagtttga agaagccgt 
      aaacatatc agttttggt ttgttgttt ctggtcttt 
      gatgggcac ttgacgtac gtgataaat tttttgata 
      aaaagaatg acaatagta aaatatatt atacatacg 
      tgtaaaaag gtgtgattc gaataagtt gtaaagtaa 
      atgtttatg aagatgtgt atcatttac atgttaatg 
      ttggacctt ttttcttcg gggtagatg ttgaatgga 
      tgttgattt ttttatttt gtcttttaa ggatttgtt 
      ttgtttttt caatgactt ggttgccga tggggcaat 
      gtcacacga tttccaaat gactaagtt gtacagttg 
      ttgaatttg gaagaaagt attactgat cttttgcaa 
      acaagaagg aagaatttt atttgttag gtttatgtt 
      gttgtacat ataaaggcc agataaaaa ttagtatct 
      gtgatttta ttaaataaa tataccaaa ttacattgt 
      atataacat gataattcc ctattttct cgagttagt 
      caattattt aagttttta taactaatg taaataggt 
      accatttac ttttataat aatataagt gttagttca 
      caatgttaa gcgaccgcc attataaat aaaactaca 
      acaaagctc gttttattt cactcaact taacccgtc 
      cctttacac ccacattct cagctctta cattaaaag 
      aaaaagatt tccattcgg ttttcttat ttttttttg 
      acgagatca tgattatta tttaactca ttcttacct 
      tgatcgttt taataaacg aggctgaat ttttgtgtg 
      taaaaagtt tattgaata aattcacaa aacggatta 
      taaagtgcg tatcgaata aacaatgca ataaattac 
      ttgtggttg ataaatttg gtcgttttc ccgttggac 
      aaaaccttg tattttggt ttgggtcga atcacttct 
      tcgttttgc acaaaattg gggcagtgg cgtcgagtg 
      tgggcataa tcgcccgta gccccacat atatcgcaa 
      atatgccgt cttaaaatc ggacaagta actctccca 
      tcttcggct tttacagta tgcgattga aatacttca 
      ggcgtttca cagtttttc caacacaga gtgcagatt 
      ttcggtcgt tgataattt tcaacatct tgcgaaaat 
      tgttgattt tggaatttt attggttgg ttttgatag 
      aaactggga gatggtggt ggtggttga acattttcg 
      atgcttcgt aaaacgttg gaaaactgt tgatttcga 
      agtgttggg gagtcgtaa cattttggg gttttgtta 
      taattattg aaagacact ggaaataag cttagtggc 
      tgaggtcac tactaatta cgaattact tactttgag 
      cacgtgatg aaaacttat tagagacga ttgaggcgg 
      tttgggaac tgtgccctc aaacggtgt taaaaacag 
      actaaaatt aatttaact gatacaaag tagaggggt 
      ggtgacgac ggcgcaagt taaaaagtt cggcttttt 
      tgatggaca aagcttctt ttgattggt gccgaattc 
      tgggaatcc ctcgaacga ataacaaaa aaacaattt 
      tcaacacga aattctttt caagaaata tgttgacag 
      tcg
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