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Gene information
General information
TC001263 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13338401..13340927
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001263
    iBeetle screen iB_09695

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001263 RA
      MKHTPVINL TLSSLTIVA VFYGSWSLL TEVINTILP 
      FGLILTILL VCAVYFLRV PAPSPASVE TILGKDPAV 
      ASSDDAYVM KTVAHRGAG LDAPENSLP AFKMCHDQG 
      CDAIEFDIT LTKDGVPVV FHDNCLERM SDCNLVIGE 
      HKWEDLCNI DISVKHLYK DRFAKTNIP TLEQTVTQL 
      LASGQRMFI DIKDNNKKF VKIIHGLFD RYPDLYSRA 
      VVSSFNPIL IYLIRKGNP KIVCSLAWR PHAFAYDSF 
      NFPMGPGIR RGKVWYKHF LLVLCDFIH EWALTRITY 
      YLLGLSVIL LHKDAINGR TVYDWRKRG LRVMAWTVN 
      TPAEKQFLA RVLKITYLT DTLTGEGTT HTPPL
    CDS sequence
    • >TC001263 RA
      atgaaacac acccctgtt atcaacctg accctttca 
      tcccttacc atcgtcgcg gttttttac ggctcatgg 
      agccttctc acggaagtt atcaacaca attttacca 
      ttcggactg attctcaca atcctgtta gtgtgtgcc 
      gtttatttc ctccgtgtc ccagcccct agtcctgca 
      tccgtcgaa acaatttta ggaaaggac cccgcagta 
      gcgtccagt gacgacgcc tacgttatg aagacagtg 
      gcgcacagg ggcgccggc ttggacgcc cccgaaaac 
      agtctgcct gctttcaaa atgtgtcac gatcaaggg 
      tgcgacgct attgagttt gacattact ttgaccaaa 
      gatggtgtt cctgttgtc tttcacgac aattgcttg 
      gagaggatg agtgactgt aatttagtc attggggaa 
      cataagtgg gaggatttg tgcaatatt gacatatca 
      gtaaagcat ttgtataag gacagattt gccaaaact 
      aatattccc acattagaa caaaccgta actcagctt 
      ctagcttca ggccaaagg atgttcatt gatattaaa 
      gacaataac aaaaagttt gtcaaaata atccacggc 
      cttttcgac cgctaccct gacctctac tcaagagcc 
      gtagtctcc tcctttaac ccaatttta atctacctt 
      atcaggaaa gggaaccca aaaatcgtt tgttccctt 
      gcttggaga cctcatgcg tttgcttac gactcgttt 
      aattttcca atggggcct ggcattcgc cgcggcaaa 
      gtttggtac aaacatttc ttgctagtt ttgtgcgat 
      ttcatacac gaatgggcc ttgaccaga atcacgtat 
      tatcttctg ggattgtcg gttattttg ttgcataag 
      gatgccatc aatggacgt acggtctac gattggcgc 
      aaaagggga ttgagggtg atggcttgg acggtaaat 
      acgccggct gaaaagcaa tttcttgca cgtgtgctt 
      aaaattact tatcttact gatacttta acgggtgaa 
      gggactaca catactcct cctttgtaa
    mRNA sequence
    • >TC001263 RA
      agctatgtg tgttagtgt gggggcggt tgataacag 
      cgggcattg ttttcttat caacatcca aacaaactg 
      cataccact gcaaatttt cctctcctt catgtgcct 
      tcgaactat aatcgttac gtaacccca cctatccac 
      aaacatgaa acacacccc tgttatcaa cctgaccct 
      ttcatccct taccatcgt cgcggtttt ttacggctc 
      atggagcct tctcacgga agttatcaa cacaatttt 
      accattcgg actgattct cacaatcct gttagtgtg 
      tgccgttta tttcctccg tgtcccagc ccctagtcc 
      tgcatccgt cgaaacaat tttaggaaa ggaccccgc 
      agtagcgtc cagtgacga cgcctacgt tatgaagac 
      agtggcgca caggggcgc cggcttgga cgcccccga 
      aaacagtct gcctgcttt caaaatgtg tcacgatca 
      agggtgcga cgctattga gtttgacat tactttgac 
      caaagatgg tgttcctgt tgtctttca cgacaattg 
      cttggagag gatgagtga ctgtaattt agtcattgg 
      ggaacataa gtgggagga tttgtgcaa tattgacat 
      atcagtaaa gcatttgta taaggacag atttgccaa 
      aactaatat tcccacatt agaacaaac cgtaactca 
      gcttctagc ttcaggcca aaggatgtt cattgatat 
      taaagacaa taacaaaaa gtttgtcaa aataatcca 
      cggcctttt cgaccgcta ccctgacct ctactcaag 
      agccgtagt ctcctcctt taacccaat tttaatcta 
      ccttatcag gaaagggaa cccaaaaat cgtttgttc 
      ccttgcttg gagacctca tgcgtttgc ttacgactc 
      gtttaattt tccaatggg gcctggcat tcgccgcgg 
      caaagtttg gtacaaaca tttcttgct agttttgtg 
      cgatttcat acacgaatg ggccttgac cagaatcac 
      gtattatct tctgggatt gtcggttat tttgttgca 
      taaggatgc catcaatgg acgtacggt ctacgattg 
      gcgcaaaag gggattgag ggtgatggc ttggacggt 
      aaatacgcc ggctgaaaa gcaatttct tgcacgtgt 
      gcttaaaat tacttatct tactgatac tttaacggg 
      tgaagggac tacacatac tcctccttt gtaaataaa 
      aagtgacat aatttggtg gtttttgga tgtatgggc 
      agcagcggg gtttaggct aattataag ggcagcaat 
      ttccgcatt tttacaatt ttagccaaa aacatgatc 
      tcgtttttt ctgtggttt aaaaaatga tttttttat 
      tcgcaagcg gattttaat gattaaaat taactgatt 
      ttattgtgt cataatttt gagcgtctg aaggagttc 
      aagcatgtt tccttatgc catatacaa atatattat 
      tttgtattt aggtaaagt aaataaaat tatatctat 
      gaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY