Status
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Gene information
General information
TC000301 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13262188..13267719
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000301
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09693

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber mostlynot present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber potentiallydegenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000301 RA
      MDKIRVLFI LPANCKDQS EASDIMLQA YESCKQNVV 
      NVSWVEEAN FSTIDFSKT DFVVFQDFE GKNFESLKE 
      SKCARILGP WCLLVCLMD GRPIPNYTW PIYNVAMAG 
      CVVSCSHIP KARKLEIKE KVQLMGGCC TDQLMSTNT 
      HLITESVKS EKYLQAAER GLKLMVSQW VEAVWEESQ 
      TNNIHCDDE KFKKYRCLP FHNLIICST GFPNTEMRA 
      EAAQNVAKN GGIFTASLT VAKTDVLIV YGVGGVLSK 
      KYKAARAHS NIYCVSIDW LNDSIEKGY ALPHALYTV 
      KKGTSTPTK EDETCPDFS IISAIGESN TTKHTMLEE 
      TVMAKKVQP SFLTTNRIS QNKRKCAGN EEMGRIIDG 
      LDIKKAKMA GQYLDGCSV YLAGFKPDQ REKLAKILN 
      LSGATRYDD ISDRLTHII VGDTSCPEV KIVRAKNYS 
      VSLVSIHWL LTSMEQQQA ADEENYLIN LHDSDREQF 
      SSPLGRKGL TLLRSSKTL TSNDLEAAN VSATSPCDA 
      ELESDALMK QYRKTVTND DTDSLMRLI KDDSKFNLD 
      ALEGRNSDV SSQNSDQTN QDTNVFIGK KFLLVNFPV 
      DESQFLKEQ IEGALGTIM PKTYKGIPD YVVAPNFIK 
      TPIQTSALE TVNSIWVCE SIHESDEVP IAYYHRPFV 
      ICDSAPLEN CVVTISGYS SFERNFLKE LIEALGGTS 
      QEQFARVLC VEKNLQAST HLVSFEADG KKYAAAVKW 
      GLPVVTKNW LFECAKSGK RVPEDEFLV GEAKAPTRP 
      VLTSSKQFT PISMSGGRK MTPLSQIQP LDISTSEKT 
      PTNPRVFNG VVETPDPCS QVTPINKLM EEVRKTNLL 
      GTPQTPPMP KTWYDVDTP ESPFGAFIR PHLSPKSKK 
      ELMRYINRI PDFVPPPQE RKSTPLSEV KRRCWRKLL 
      GQQGQDSPI SSKVQDEAC ASRAECEIN DENSGDDVK 
      NSSQAESVT DNTSEANTQ NLFVNTKLR EIEDMVRTI 
      NDNRRKSRP FQSEVPAEV GSSAVKDSQ PFTVGWDYN 
      EEPPIQQVR IFMISGMSN EERQAIVTK IEALGGQVS 
      DLNSFDPKC THLICPKPA RNEKTLSCM ASGKWILHA 
      SYVDKCASA GKFLPEEEF EFGNPKARD NIKVFDKEN 
      EFKMQSVHW WRREIKRRG YGAFHDMRA IVVAQKKEP 
      IVRVIEAGG GQVLDVAPP FKENVHATH CLLEIKSVP 
      DFSAYLPLA QQGILCLNT LYISDFLYR TNKDVKDAI 
      IPHFTKFYT
    CDS sequence
    • >TC000301 RA
      atggacaaa atccgggtg ctttttatc ctgcctgcg 
      aattgcaaa gaccagagt gaggcctcg gacatcatg 
      cttcaagcc tacgagtct tgcaaacaa aacgtggta 
      aacgtgtct tgggtggaa gaagcgaat tttagcaca 
      attgatttt tccaaaacc gattttgtg gttttccaa 
      gactttgaa ggcaaaaat tttgagtcc ctcaaagag 
      agcaagtgt gccagaatt ttgggacct tggtgtctc 
      ttagtttgt ttgatggat ggtcgcccc atcccaaac 
      tacacatgg cccatttac aacgtcgca atggcaggc 
      tgcgtcgtt tcgtgctcg cacatcccc aaagcgaga 
      aaactcgaa attaaggaa aaagtccaa ctaatgggg 
      ggctgttgc accgaccag ttaatgtca actaacaca 
      catttaatt acagagagc gtcaagtct gagaagtat 
      ttgcaagca gctgagcgg ggcttgaaa ttgatggtt 
      tcgcagtgg gtggaagcc gtttgggag gaaagtcaa 
      acgaataac atacattgc gacgacgaa aagttcaaa 
      aaataccgc tgtttgccg tttcacaat ctcataatt 
      tgctcaacc ggctttccc aacactgaa atgagggca 
      gaagcggcc caaaacgtt gccaaaaac ggtggtatt 
      ttcacggcg tctctgacc gtcgcaaag actgatgtt 
      ttaatcgta tatggggtc ggtggtgtt ttgagcaaa 
      aaatacaag gcagcaagg gcacactct aacatttat 
      tgcgtgagc attgattgg ttgaatgat agtatagaa 
      aagggctac gccctgccc cacgcttta tacacggtt 
      aaaaagggc acttcaacg cccactaaa gaggacgag 
      acttgcccc gatttttca ataatcagt gccataggg 
      gagagcaat actacgaaa catacaatg ctcgaagaa 
      actgtgatg gcgaaaaaa gtacaaccg tcatttctt 
      acaacaaat cggatttcg caaaataaa agaaaatgc 
      gcgggtaac gaggaaatg gggagaatt attgacgga 
      cttgatatc aaaaaggcc aaaatggca ggacagtat 
      ttggacggg tgcagtgtc tacttggcc ggcttcaag 
      ccggatcag agagaaaag ctcgcaaaa atcctaaat 
      ctgagcggt gccacgaga tacgacgac atctccgac 
      cgcctcact cacataatc gtcggcgac acgtcttgt 
      cccgaagtg aaaatagtg cgagcaaaa aattactca 
      gtctcgctt gtctccatc cactggctt ttgaccagt 
      atggagcaa cagcaagcc gcagatgag gaaaattac 
      ctaattaat ttacacgat tctgatcgc gagcaattc 
      agttcgcca cttggacga aaaggcctc actttactc 
      agatctagc aaaacgctc acttcgaac gatctggaa 
      gctgcaaat gtgtcggcg accagtccg tgtgacgcg 
      gaactggaa agcgatgct ttaatgaag cagtatcgc 
      aaaacggtg acaaacgat gatactgat tctttaatg 
      cggttgatc aaagacgac agtaaattt aatttggac 
      gctttggaa ggacgcaat tcagacgtg tcgagtcaa 
      aattcggat caaacgaat caagacacg aatgttttc 
      attggcaaa aaattcctt ctggtgaat ttcccagtt 
      gatgagtcg caatttctg aaggaacag attgagggc 
      gcccttggc acaatcatg ccaaagacg tacaaaggc 
      attccggat tacgttgtc gccccaaat tttatcaaa 
      accccgata caaacctcc gcacttgaa acagtcaac 
      agtatatgg gtgtgcgag tcgattcac gaaagtgac 
      gaggttccc atagcctac taccaccgc ccctttgtc 
      atttgcgac tccgcacct ctcgaaaat tgcgtcgtc 
      acaatcagt ggttactca tcctttgag cgaaatttc 
      ctgaaagag ttgatcgaa gccctgggc gggacgtcc 
      caagaacag tttgccaga gtcctctgt gttgaaaaa 
      aacctccaa gcttcgacc cacttggtc agtttcgaa 
      gcagacggc aaaaaatac gcagccgct gtgaaatgg 
      gggctgcca gtggtcaca aaaaactgg ctgtttgag 
      tgcgccaaa agcgggaaa cgcgtacca gaagacgag 
      tttttggtc ggcgaagcc aaagcaccc actaggcct 
      gtcctgact tcaagcaaa caatttacg cctatttcc 
      atgtcaggt ggccgcaaa atgacaccc ttgagccaa 
      attcaaccg ttagatatt agcacgtct gaaaaaacc 
      ccaacaaac ccccgtgtt tttaacgga gttgtcgag 
      acgccagat ccgtgcagt caagtgact ccgatcaac 
      aaattaatg gaggaagtt cgcaaaact aatctttta 
      gggactccg caaacacca ccgatgccg aagacttgg 
      tatgatgtt gatacgccc gaaagtccg ttcggtgcg 
      tttattcga ccgcacttg tcgccgaaa tcgaaaaaa 
      gagttgatg cgttacatc aatcgcata ccggatttt 
      gtgccgccg ccccaggaa cgaaaatcg actccgtta 
      tctgaagtc aagcggcgt tgttggaga aaattgtta 
      gggcaacag ggacaggac agcccaatt tctagcaag 
      gtgcaagat gaggcttgt gctagtcga gcagaatgc 
      gaaataaat gatgaaaat agtggcgat gatgtgaag 
      aattcgtct caagctgag agtgtcact gataacact 
      tctgaggcc aacactcaa aatttgttt gtaaacacg 
      aaacttagg gaaattgag gacatggtg cggacaatt 
      aacgacaac cggagaaaa agtcgcccg tttcagtca 
      gaagtgccg gcggaagtt ggtagctcg gcagttaaa 
      gactcgcaa ccgttcaca gtcggctgg gattataac 
      gaagaacca ccgattcaa caagtccgc attttcatg 
      atctcgggc atgagtaac gaggagcga caggcaatt 
      gtcacaaaa atcgaggct cttgggggc caagtgtcc 
      gacttgaat tcattcgac ccgaagtgc actcacttg 
      atttgcccc aagccggcg cggaacgaa aaaaccctc 
      tcctgtatg gcgtcggga aaatggata cttcacgct 
      tcatacgtg gacaagtgt gcctcagcg ggaaaattc 
      ctcccggag gaggagttc gagtttggc aatcccaag 
      gccagggac aatattaaa gttttcgat aaagaaaac 
      gagtttaag atgcagtcg gtgcactgg tggcggcgc 
      gaaatcaag aggagaggg tacggcgca tttcacgat 
      atgcgagcc attgttgtt gcccagaag aaagagccg 
      atagttagg gtgattgag gcgggcggg gggcaggtg 
      ctggatgtg gcgcctccg tttaaggag aacgttcac 
      gccactcac tgccttttg gaaattaaa agtgtcccc 
      gattttagt gcttatctg ccgttggcc cagcaaggg 
      attttgtgt ctgaacacg ctgtatatt agtgatttt 
      ttatatagg accaataag gatgttaag gacgcgatt 
      attccacat tttaccaag ttctacacg tga
    mRNA sequence
    • >TC000301 RA
      aaatgatca tgacagttg attttaacc acaaaaaag 
      tgatgtttt ttatttaat attgaatgt tttctgata 
      tttttattg tggttttat tgtaacata acgtgtcga 
      ttctgtggt gttagtaat ttggtaaaa aaggcgcat 
      ttcataatt ttatcataa gaaagttat tggttattg 
      gcggtaaaa ttgtcttgc aaaatggac aaaatccgg 
      gtgcttttt atcctgcct gcgaattgc aaagaccag 
      agtgaggcc tcggacatc atgcttcaa gcctacgag 
      tcttgcaaa caaaacgtg gtaaacgtg tcttgggtg 
      gaagaagcg aattttagc acaattgat ttttccaaa 
      accgatttt gtggttttc caagacttt gaaggcaaa 
      aattttgag tccctcaaa gagagcaag tgtgccaga 
      attttggga ccttggtgt ctcttagtt tgtttgatg 
      gatggtcgc cccatccca aactacaca tggcccatt 
      tacaacgtc gcaatggca ggctgcgtc gtttcgtgc 
      tcgcacatc cccaaagcg agaaaactc gaaattaag 
      gaaaaagtc caactaatg gggggctgt tgcaccgac 
      cagttaatg tcaactaac acacattta attacagag 
      agcgtcaag tctgagaag tatttgcaa gcagctgag 
      cggggcttg aaattgatg gtttcgcag tgggtggaa 
      gccgtttgg gaggaaagt caaacgaat aacatacat 
      tgcgacgac gaaaagttc aaaaaatac cgctgtttg 
      ccgtttcac aatctcata atttgctca accggcttt 
      cccaacact gaaatgagg gcagaagcg gcccaaaac 
      gttgccaaa aacggtggt attttcacg gcgtctctg 
      accgtcgca aagactgat gttttaatc gtatatggg 
      gtcggtggt gttttgagc aaaaaatac aaggcagca 
      agggcacac tctaacatt tattgcgtg agcattgat 
      tggttgaat gatagtata gaaaagggc tacgccctg 
      ccccacgct ttatacacg gttaaaaag ggcacttca 
      acgcccact aaagaggac gagacttgc cccgatttt 
      tcaataatc agtgccata ggggagagc aatactacg 
      aaacataca atgctcgaa gaaactgtg atggcgaaa 
      aaagtacaa ccgtcattt cttacaaca aatcggatt 
      tcgcaaaat aaaagaaaa tgcgcgggt aacgaggaa 
      atggggaga attattgac ggacttgat atcaaaaag 
      gccaaaatg gcaggacag tatttggac gggtgcagt 
      gtctacttg gccggcttc aagccggat cagagagaa 
      aagctcgca aaaatccta aatctgagc ggtgccacg 
      agatacgac gacatctcc gaccgcctc actcacata 
      atcgtcggc gacacgtct tgtcccgaa gtgaaaata 
      gtgcgagca aaaaattac tcagtctcg cttgtctcc 
      atccactgg cttttgacc agtatggag caacagcaa 
      gccgcagat gaggaaaat tacctaatt aatttacac 
      gattctgat cgcgagcaa ttcagttcg ccacttgga 
      cgaaaaggc ctcacttta ctcagatct agcaaaacg 
      ctcacttcg aacgatctg gaagctgca aatgtgtcg 
      gcgaccagt ccgtgtgac gcggaactg gaaagcgat 
      gctttaatg aagcagtat cgcaaaacg gtgacaaac 
      gatgatact gattcttta atgcggttg atcaaagac 
      gacagtaaa tttaatttg gacgctttg gaaggacgc 
      aattcagac gtgtcgagt caaaattcg gatcaaacg 
      aatcaagac acgaatgtt ttcattggc aaaaaattc 
      cttctggtg aatttccca gttgatgag tcgcaattt 
      ctgaaggaa cagattgag ggcgccctt ggcacaatc 
      atgccaaag acgtacaaa ggcattccg gattacgtt 
      gtcgcccca aattttatc aaaaccccg atacaaacc 
      tccgcactt gaaacagtc aacagtata tgggtgtgc 
      gagtcgatt cacgaaagt gacgaggtt cccatagcc 
      tactaccac cgccccttt gtcatttgc gactccgca 
      cctctcgaa aattgcgtc gtcacaatc agtggttac 
      tcatccttt gagcgaaat ttcctgaaa gagttgatc 
      gaagccctg ggcgggacg tcccaagaa cagtttgcc 
      agagtcctc tgtgttgaa aaaaacctc caagcttcg 
      acccacttg gtcagtttc gaagcagac ggcaaaaaa 
      tacgcagcc gctgtgaaa tgggggctg ccagtggtc 
      acaaaaaac tggctgttt gagtgcgcc aaaagcggg 
      aaacgcgta ccagaagac gagtttttg gtcggcgaa 
      gccaaagca cccactagg cctgtcctg acttcaagc 
      aaacaattt acgcctatt tccatgtca ggtggccgc 
      aaaatgaca cccttgagc caaattcaa ccgttagat 
      attagcacg tctgaaaaa accccaaca aacccccgt 
      gtttttaac ggagttgtc gagacgcca gatccgtgc 
      agtcaagtg actccgatc aacaaatta atggaggaa 
      gttcgcaaa actaatctt ttagggact ccgcaaaca 
      ccaccgatg ccgaagact tggtatgat gttgatacg 
      cccgaaagt ccgttcggt gcgtttatt cgaccgcac 
      ttgtcgccg aaatcgaaa aaagagttg atgcgttac 
      atcaatcgc ataccggat tttgtgccg ccgccccag 
      gaacgaaaa tcgactccg ttatctgaa gtcaagcgg 
      cgttgttgg agaaaattg ttagggcaa cagggacag 
      gacagccca atttctagc aaggtgcaa gatgaggct 
      tgtgctagt cgagcagaa tgcgaaata aatgatgaa 
      aatagtggc gatgatgtg aagaattcg tctcaagct 
      gagagtgtc actgataac acttctgag gccaacact 
      caaaatttg tttgtaaac acgaaactt agggaaatt 
      gaggacatg gtgcggaca attaacgac aaccggaga 
      aaaagtcgc ccgtttcag tcagaagtg ccggcggaa 
      gttggtagc tcggcagtt aaagactcg caaccgttc 
      acagtcggc tgggattat aacgaagaa ccaccgatt 
      caacaagtc cgcattttc atgatctcg ggcatgagt 
      aacgaggag cgacaggca attgtcaca aaaatcgag 
      gctcttggg ggccaagtg tccgacttg aattcattc 
      gacccgaag tgcactcac ttgatttgc cccaagccg 
      gcgcggaac gaaaaaacc ctctcctgt atggcgtcg 
      ggaaaatgg atacttcac gcttcatac gtggacaag 
      tgtgcctca gcgggaaaa ttcctcccg gaggaggag 
      ttcgagttt ggcaatccc aaggccagg gacaatatt 
      aaagttttc gataaagaa aacgagttt aagatgcag 
      tcggtgcac tggtggcgg cgcgaaatc aagaggaga 
      gggtacggc gcatttcac gatatgcga gccattgtt 
      gttgcccag aagaaagag ccgatagtt agggtgatt 
      gaggcgggc ggggggcag gtgctggat gtggcgcct 
      ccgtttaag gagaacgtt cacgccact cactgcctt 
      ttggaaatt aaaagtgtc cccgatttt agtgcttat 
      ctgccgttg gcccagcaa gggattttg tgtctgaac 
      acgctgtat attagtgat tttttatat aggaccaat 
      aaggatgtt aaggacgcg attattcca cattttacc 
      aagttctac acgtgacct gttgtgttg tgttcagat 
      aattaataa ataaatgtg ttctttcgt tttattttg 
      tatattcgt cgatctttt tttataaat acatgatta 
      atttgcaa
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