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Gene information
General information
TC000303 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13250984..13253629
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000303
    iBeetle screen iB_09692

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000303 RA
      MAKISMEIE TKKDVPFEE AISKTGYGF FNLAILLAS 
      GLGLMCVIV ETMMMMFIV PAAQCDLDL SLSEKGLLS 
      SVSFIGVVV SSYMWGYLA DTKGRKIVL ELSLSISAV 
      TTVVCSVVP WVWLFILLR LVNGLAIGG ASSAVYAYC 
      GEFHDNHYR PKVISWMST FVACGNMFL PGLAWIILP 
      ADWEIDLPV FGIIYKPWR LLLILYTLP CLICAVFIH 
      FLPESPKFL LTQDRKREA LDILKKMFV INKRKSEDF 
      YGVENIIWE ELSGSELKK KQGMLRTMW KQTVPLFKG 
      ELLVKTLMM CYLQFGVFA SNSGIVMWY PEILNRMSH 
      YVEDHSESD VSLCSAILS EQPHDNNTL TDVLKVCND 
      QVKPRVFLV SLLMGVAYA MIYIFIGSV INIFGKKQL 
      MISFVSVTT ITGLASQYV YGYNLIQFL VGIFLMGGA 
      GIGITNAIA VDLFPTQVR GMALAVSLM FGRIGAMTG 
      TNIVGPLIY KFCDYLFYF VAADHLILI IVVLLLPSS 
      PRLPKLTL
    CDS sequence
    • >TC000303 RA
      atggcaaag ataagcatg gaaattgaa acgaaaaaa 
      gatgtgcct tttgaggaa gccatttcc aagaccggt 
      tacggcttt ttcaacctt gccattttg ctagcctct 
      ggcttgggc ctcatgtgc gtcatcgtc gagacaatg 
      atgatgatg ttcattgta ccggcggcc caatgcgac 
      ctcgacctc agcctttcc gagaaaggt ctcttatca 
      tcagtcagc ttcatcgga gttgtagtt agttcctac 
      atgtggggc tatttggcc gacaccaaa ggccgcaaa 
      attgtcctc gaattgagt ctctcgatc agcgccgtc 
      accaccgtc gtttgcagt gtagtgcca tgggtctgg 
      ctcttcatc ctcctccgt ctagtcaac ggcctagct 
      attggaggc gcctcgtcc gccgtctac gcctactgt 
      ggcgagttc catgacaat cattacagg cccaaggta 
      atttcctgg atgtcgaca ttcgtggcg tgcggaaac 
      atgttcctg ccgggccta gcctggatc atcctcccc 
      gccgactgg gagattgat cttcccgtt ttcgggatc 
      atttacaag ccgtggcgg ctcctcctc atcctttac 
      accctccct tgcttgatt tgtgccgta tttatccat 
      ttcctgccc gagagtccg aaatttttg ctgacgcaa 
      gaccgcaag agggaagct ctcgatatt ttgaaaaaa 
      atgtttgtc atcaataag cgaaaaagt gaggatttc 
      tacggcgtc gagaacata atttgggaa gaactgtcc 
      ggtagcgaa ttgaaaaag aagcagggt atgttgagg 
      acgatgtgg aagcaaaca gtgccgctg ttcaagggg 
      gagcttttg gtgaagacg ctcatgatg tgttatctc 
      cagttcgga gtttttgcc tccaattct ggtattgtt 
      atgtggtat ccggagatt ctaaaccgc atgtcgcac 
      tatgtggaa gaccattcg gagagcgac gtttctttg 
      tgttcggca atactttcg gagcaacca cacgacaat 
      aatactcta actgatgtt ttgaaagtg tgtaacgac 
      caagtgaag ccgcgagtg tttctcgtg tcgctcctc 
      atgggggtg gcatatgcc atgatttat attttcata 
      ggaagtgtt atcaacatt ttcgggaag aaacagcta 
      atgatcagc tttgtgtcg gtaaccact atcaccgga 
      cttgcatcg caatatgtt tacggctat aatcttatc 
      caatttctt gttggaatc ttcctaatg ggcggagct 
      gggatcgga ataaccaat gccatcgca gttgatttg 
      ttcccaact caggtcaga gggatggct cttgccgtt 
      tccttgatg tttggaagg ataggcgca atgacagga 
      actaatatc gtaggacca ctgatttac aaattttgt 
      gattatctg ttttatttc gtcgccgcc gatcactta 
      atattaatt attgtggtg ttgttgctg ccgtcgtcg 
      ccacgttta ccaaaactg acgctttag
    mRNA sequence
    • >TC000303 RA
      tcattattt atgatgctt caagataat taacagtat 
      taacaccga caaaaatat ttgagttta actcgagct 
      cgtgtgcta ggaacatgg caaagataa gcatggaaa 
      ttgaaacga aaaaagatg tgccttttg aggaagcca 
      tttccaaga ccggttacg gctttttca accttgcca 
      ttttgctag cctctggct tgggcctca tgtgcgtca 
      tcgtcgaga caatgatga tgatgttca ttgtaccgg 
      cggcccaat gcgacctcg acctcagcc tttccgaga 
      aaggtctct tatcatcag tcagcttca tcggagttg 
      tagttagtt cctacatgt ggggctatt tggccgaca 
      ccaaaggcc gcaaaattg tcctcgaat tgagtctct 
      cgatcagcg ccgtcacca ccgtcgttt gcagtgtag 
      tgccatggg tctggctct tcatcctcc tccgtctag 
      tcaacggcc tagctattg gaggcgcct cgtccgccg 
      tctacgcct actgtggcg agttccatg acaatcatt 
      acaggccca aggtaattt cctggatgt cgacattcg 
      tggcgtgcg gaaacatgt tcctgccgg gcctagcct 
      ggatcatcc tccccgccg actgggaga ttgatcttc 
      ccgttttcg ggatcattt acaagccgt ggcggctcc 
      tcctcatcc tttacaccc tcccttgct tgatttgtg 
      ccgtattta tccatttcc tgcccgaga gtccgaaat 
      ttttgctga cgcaagacc gcaagaggg aagctctcg 
      atattttga aaaaaatgt ttgtcatca ataagcgaa 
      aaagtgagg atttctacg gcgtcgaga acataattt 
      gggaagaac tgtccggta gcgaattga aaaagaagc 
      agggtatgt tgaggacga tgtggaagc aaacagtgc 
      cgctgttca agggggagc ttttggtga agacgctca 
      tgatgtgtt atctccagt tcggagttt ttgcctcca 
      attctggta ttgttatgt ggtatccgg agattctaa 
      accgcatgt cgcactatg tggaagacc attcggaga 
      gcgacgttt ctttgtgtt cggcaatac tttcggagc 
      aaccacacg acaataata ctctaactg atgttttga 
      aagtgtgta acgaccaag tgaagccgc gagtgtttc 
      tcgtgtcgc tcctcatgg gggtggcat atgccatga 
      tttatattt tcataggaa gtgttatca acattttcg 
      ggaagaaac agctaatga tcagctttg tgtcggtaa 
      ccactatca ccggacttg catcgcaat atgtttacg 
      gctataatc ttatccaat ttcttgttg gaatcttcc 
      taatgggcg gagctggga tcggaataa ccaatgcca 
      tcgcagttg atttgttcc caactcagg tcagaggga 
      tggctcttg ccgtttcct tgatgtttg gaaggatag 
      gcgcaatga caggaacta atatcgtag gaccactga 
      tttacaaat tttgtgatt atctgtttt atttcgtcg 
      ccgccgatc acttaatat taattattg tggtgttgt 
      tgctgccgt cgtcgccac gtttaccaa aactgacgc 
      tttaggatg gaattatag gattaaata aataatgaa 
      ttgtacaaa cagaaccgg ctttattta gcttctttc 
      tgtacataa aaagactta atttatgac ttttatata 
      aataaaaga ttacgtttt aaataattt gaatttttt 
      gaagtactt ctggacctc gtccttggg gtacggctt 
      agccaccag acacgtagg aatgttttc cggttgttc 
      gatccacaa cttatgctt gatattatt ttcgcttaa 
      actatctct cagtttgtt taaactgcc ctcgtcggg 
      ggcctcaag tacgacctt gtgcatgtt atccaagtc 
      ttttagata catttgcac atgttcatc gtcatgaaa 
      caagtgtgt gactgctgt tacggcatg acacgcttg 
      tgcgataac agcccccac gggccaccc cagttcttt 
      taaaaggtc ccccctcac aacaacata ctggacaat 
      cccattcat ttctacgaa aaaatattc aatgtcaat 
      gacattgat tatgtctaa gactgtaca ttttgcaat 
      cgggcaaaa aataaaaag aatgcggat at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY