Status
Please wait ...
Gene information
General information
TC001245 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13177632..13196802
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001245
    iBeetle screen iB_09690

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001245 RA
      MGEEHYDED SKSNFSVLN ETLDGFANE TVSPDVLFQ 
      QNLTVILVY SALFVVAAV GNLTVFISL FRSRHRKSR 
      ISLMIRHLA IADLIVTFI MIPIEVGWR LTGKWIAGN 
      VACKVFLFL RAFGPYLSS NVLVCVSLD RYFAVLHPL 
      RVNDARRRG KIMLAFAWG TSFVYCIPQ SFVFRVRAH 
      PKYPNYEQC VSFGFFENT AQEIAYNLM CVMCMYFIP 
      LFVIIVAYT AIMCEISKN SKETKGESY RTSNGRMRL 
      RRSDISNIE RARSRTLRM TITIVAVYV WCCTPYVII 
      TMWYMFDRA SATSLPEWL QDTFFMMVV SNSCMNPIV 
      YGSYVINFQ RVNCNCFCF RKTASESHL NDGEAQAAA 
      GRGRSLARG VIKCVLRLQ KVGSGATRS TAMVHGAGG 
      NGYTRSPTP KSNLNLTGL LSKSRLPDK PPSVGHISF 
      LSEPRTARN YRSSFHSEP CSRTRMCPD ELCLDTSCH 
      SADYYSSAV L
    CDS sequence
    • >TC001245 RA
      atgggcgag gaacattat gatgaggac tctaaaagt 
      aattttagt gtgttgaac gaaactttg gacgggttt 
      gctaacgag acggtctct cctgacgta cttttccaa 
      cagaacttg acagtcatt ctcgtttat agtgcgctt 
      ttcgtcgtc gccgctgtc gggaacttg actgttttt 
      atttccctg ttcaggtcg agacaccgc aaatcaaga 
      attagttta atgattcgc catcttgcc atcgctgat 
      ttgattgta acttttatt atgattcca atcgaggtt 
      ggctggaga ttgactggc aaatggatc gctggcaat 
      gtggcatgc aaagtattc ttatttctt cgggctttc 
      gggccctac cttagcagc aacgtgctt gtgtgcgtg 
      tccttggac cggtacttt gctgtcctg cacccgctc 
      agagtcaac gacgccaga aggagaggc aagataatg 
      ctcgccttc gcatgggga acgtcgttc gtttactgt 
      ataccacag agctttgtg tttcgggtg cgggcacac 
      cccaagtac ccgaactac gagcaatgc gtctcgttt 
      ggtttcttc gagaacacc gcccaagaa atcgcctac 
      aatctgatg tgcgttatg tgcatgtat ttcattcct 
      ctgttcgtc atcatcgtc gcttacacc gccatcatg 
      tgcgaaatc tccaaaaac tccaaagaa accaaggga 
      gagtcatat cgtacttca aatggcagg atgagacta 
      aggcgctcc gatattagc aatatagaa agggctagg 
      agcagaacc ttaaggatg accatcact attgttgca 
      gtctatgtc tggtgctgc acaccgtat gttattatc 
      accatgtgg tacatgttt gatagagca agcgccacg 
      agcctaccg gagtggctc caggacaca tttttcatg 
      atggtagtt tccaacagt tgcatgaac cccatagtt 
      tacgggagc tacgtgatc aattttcag cgagttaac 
      tgcaactgc ttttgcttc aggaagaca gcgagcgaa 
      agtcatctc aacgatgga gaggcgcag gcggcggca 
      gggcgggga aggagttta gcgcgggga gttataaaa 
      tgcgtcctc agattgcag aaagtgggc tcgggggcc 
      accaggagc acggccatg gtccatggc gcgggcggc 
      aacgggtac acacgctca ccgaccccc aagtccaac 
      ctgaatcta acagggctc ctaagcaag agccggtta 
      cctgacaaa cccccgagt gttggtcat atttcgttc 
      ctgagcgag cctcgcacc gccaggaat tacagatcg 
      agttttcat agcgagccg tgctcaaga actcgaatg 
      tgcccggat gaactttgt ctcgacact tcatgccac 
      agcgctgat tattacagt tctgctgtt ttatag
    mRNA sequence
    • >TC001245 RA
      gcgcttatt taaaacccc atcaagttt ttaacgcaa 
      tgtgacaaa aatgctcgt aatcgcgtt tcatgcaag 
      cggttggta aaatgggcg aggaacatt atgatgagg 
      actctaaaa gtaatttta gtgtgttga acgaaactt 
      tggacgggt ttgctaacg agacggtct ctcctgacg 
      tacttttcc aacagaact tgacagtca ttctcgttt 
      atagtgcgc ttttcgtcg tcgccgctg tcgggaact 
      tgactgttt ttatttccc tgttcaggt cgagacacc 
      gcaaatcaa gaattagtt taatgattc gccatcttg 
      ccatcgctg atttgattg taactttta ttatgattc 
      caatcgagg ttggctgga gattgactg gcaaatgga 
      tcgctggca atgtggcat gcaaagtat tcttatttc 
      ttcgggctt tcgggccct accttagca gcaacgtgc 
      ttgtgtgcg tgtccttgg accggtact ttgctgtcc 
      tgcacccgc tcagagtca acgacgcca gaaggagag 
      gcaagataa tgctcgcct tcgcatggg gaacgtcgt 
      tcgtttact gtataccac agagctttg tgtttcggg 
      tgcgggcac accccaagt acccgaact acgagcaat 
      gcgtctcgt ttggtttct tcgagaaca ccgcccaag 
      aaatcgcct acaatctga tgtgcgtta tgtgcatgt 
      atttcattc ctctgttcg tcatcatcg tcgcttaca 
      ccgccatca tgtgcgaaa tctccaaaa actccaaag 
      aaaccaagg gagagtcat atcgtactt caaatggca 
      ggatgagac taaggcgct ccgatatta gcaatatag 
      aaagggcta ggagcagaa ccttaagga tgaccatca 
      ctattgttg cagtctatg tctggtgct gcacaccgt 
      atgttatta tcaccatgt ggtacatgt ttgatagag 
      caagcgcca cgagcctac cggagtggc tccaggaca 
      catttttca tgatggtag tttccaaca gttgcatga 
      accccatag tttacggga gctacgtga tcaattttc 
      agcgagtta actgcaact gcttttgct tcaggaaga 
      cagcgagcg aaagtcatc tcaacgatg gagaggcgc 
      aggcggcgg cagggcggg gaaggagtt tagcgcggg 
      gagttataa aatgcgtcc tcagattgc agaaagtgg 
      gctcggggg ccaccagga gcacggcca tggtccatg 
      gcgcgggcg gcaacgggt acacacgct caccgaccc 
      ccaagtcca acctgaatc taacagggc tcctaagca 
      agagccggt tacctgaca aacccccga gtgttggtc 
      atatttcgt tcctgagcg agcctcgca ccgccagga 
      attacagat cgagttttc atagcgagc cgtgctcaa 
      gaactcgaa tgtgcccgg atgaacttt gtctcgaca 
      cttcatgcc acagcgctg attattaca gttctgctg 
      ttttatagg aacaggaga tgtcaccca agtgtgaca 
      tcaaaatgg gcattaatg gtgcagtgg cgctgtata 
      gttttgaac ccaggtcgc ggaatattt agtggttgg 
      aggagggac aagtgcctg agaagtgga cacgggaat 
      acatgtgct gatttcata acagggttt ggacatgta 
      catagtcga ttttttatt tttctattt gttaattca 
      tgtttcatt aggcaggta attaactac tcactgttt 
      aaactaaat atcaaacaa ggaccgaca actggattg 
      tgtgtacct atctactaa atgtttaaa aaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY