Status
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Gene information
General information
TC032219
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030781
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13041420..13065603
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032219
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09689

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva completeeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032219 RA
      MYLGNVVSA GTISSPWTP VTGPPADSN GAGPDTKNL 
      DVGDVSDDE KDMTAADAE GVWSPDIEQ SFQEALAIY 
      PPCGRRKII LSDEGKMYG RNELIARYI KLRTGKTRT 
      RKQVSSHIQ VLARRKLRE IQAKLKVQF WQPGLQPGT 
      SQDVKPFTQ SSYPAGKPA TAVSAGDVG PLQTAPPPV 
      WEGRAIATH KLRLVEYSA FMESQNRDE AYQRHLFVH 
      IGGPALSYT DPLLEAVDV RQIYDKFPE KKGGLKELY 
      DKGPQNAFF LVKFWADLN SNIQDEAGA FYGVTSSYE 
      SNENMTITC STKVCSFGK QVVEKVETE YARYENGRF 
      VYRIHRSPM CEYMINFIH KLKHLPEKY MMNSVLENF 
      TILQVVSNR DTQETLLCT AYVFEVSTS EHGAQHHIY 
      RLVKD
    CDS sequence
    • >TC032219 RA
      atgtattta ggcaacgtg gtttcagcc ggcaccatt 
      tcctcgccg tggacgccc gtcaccggc ccgcccgcc 
      gactccaac ggcgcgggt ccggataca aagaatcta 
      gatgttgga gacgtcagc gatgatgaa aaggatatg 
      acagcggca gatgctgaa ggagtatgg agtccggat 
      atcgagcag agttttcag gaagcgtta gctatatat 
      cctccgtgc ggtcgacgg aaaatcata ttatcagac 
      gaaggaaaa atgtatggt cggaacgaa ctcatagcg 
      cgatatatc aaattaagg actggaaaa acacgtaca 
      agaaaacaa gtcagttca cacatacag gttttggcc 
      aggcgaaaa ctgagggaa atccaagcc aaattgaag 
      gtacagttc tggcagccg ggcttgcaa ccaggaacc 
      agtcaagat gttaaaccg tttacacag tcgtcgtat 
      cctgctggc aagcctgcg acagcagtc tctgcagga 
      gacgttgga ccgctgcaa acagcccca cctccggtc 
      tgggaaggt cgggccata gccacacat aaacttaga 
      cttgttgaa tattcggcc tttatggaa tcgcaaaac 
      agagacgaa gcatatcag aggcactta ttcgttcat 
      ataggcggc ccggcttta tcatacacc gatcccttg 
      cttgaggcg gtggacgtc cgacaaatc tacgacaaa 
      ttcccggag aagaaagga ggtttgaag gaactgtac 
      gacaaagga ccccagaac gccttcttc cttgtcaaa 
      ttctgggct gatttgaat tccaatatt caagacgag 
      gctggtgcc ttttacggt gtgacgagt tcgtacgaa 
      agtaacgaa aatatgacc atcacatgt tcgacgaaa 
      gtgtgttcg tttggtaaa caagtggtg gaaaaggtg 
      gagacggaa tacgcccgt tacgaaaac ggccgattc 
      gtttatagg atccaccga agtcccatg tgcgaatat 
      atgatcaat ttcatacac aaactcaaa catctaccc 
      gaaaagtac atgatgaac agcgtctta gaaaatttc 
      accatacta caggtggtg agcaatcga gacacgcaa 
      gagacctta ctgtgtacg gcgtacgtg ttcgaggtg 
      tcgacgtca gaacacggc gcccaacat cacatctac 
      aggcttgta aaagactag
    mRNA sequence
    • >TC032219 RA
      cgctgtagc cgttgcgtt gtctagatt ctttgtacc 
      attcctggc ataccaata acagccaaa ctcctcata 
      tttacggac caaaatccg tcctaatta tccccagaa 
      tcggcgcag ggccccgca gtaatattt ttggctaaa 
      tatccgtta gaatgtgag caatgtgtc cacaattcc 
      tccaatact ttacatact tgtcatgct tgctgttac 
      gaagttctg acgactgac atcgtcggc gggtctcgg 
      tcttgcctc aatttcgaa cgggctttt cgcgaaaat 
      tttctctgc ttttttttg ctctttgat cgggtggaa 
      aacgcgtgt ttaccattg ggtcgatcg atccgacca 
      gcgagtgaa ttgtgaaat tgttgtgct ggacatgtt 
      gtgcgctcg ctgaccgtt tcttattat gaatcgaca 
      ctttgttta caacaatcg acagtggtg tttgtttgg 
      ggtgccgtt tgtgcaatg attttgatc gacagtgaa 
      cggtggtga ctgtgacgt ctgccgacg gactatttg 
      gattataac cacctgttc ataacacca aacggggga 
      tgtatttag gcaacgtgg tttcagccg gcaccattt 
      cctcgccgt ggacgcccg tcaccggcc cgcccgccg 
      actccaacg gcgcgggtc cggatacaa agaatctag 
      atgttggag acgtcagcg atgatgaaa aggatatga 
      cagcggcag atgctgaag gagtatgga gtccggata 
      tcgagcaga gttttcagg aagcgttag ctatatatc 
      ctccgtgcg gtcgacgga aaatcatat tatcagacg 
      aaggaaaaa tgtatggtc ggaacgaac tcatagcgc 
      gatatatca aattaagga ctggaaaaa cacgtacaa 
      gaaaacaag tcagttcac acatacagg ttttggcca 
      ggcgaaaac tgagggaaa tccaagcca aattgaagg 
      tacagttct ggcagccgg gcttgcaac caggaacca 
      gtcaagatg ttaaaccgt ttacacagt cgtcgtatc 
      ctgctggca agcctgcga cagcagtct ctgcaggag 
      acgttggac cgctgcaaa cagccccac ctccggtct 
      gggaaggtc gggccatag ccacacata aacttagac 
      ttgttgaat attcggcct ttatggaat cgcaaaaca 
      gagacgaag catatcaga ggcacttat tcgttcata 
      taggcggcc cggctttat catacaccg atcccttgc 
      ttgaggcgg tggacgtcc gacaaatct acgacaaat 
      tcccggaga agaaaggag gtttgaagg aactgtacg 
      acaaaggac cccagaacg ccttcttcc ttgtcaaat 
      tctgggctg atttgaatt ccaatattc aagacgagg 
      ctggtgcct tttacggtg tgacgagtt cgtacgaaa 
      gtaacgaaa atatgacca tcacatgtt cgacgaaag 
      tgtgttcgt ttggtaaac aagtggtgg aaaaggtgg 
      agacggaat acgcccgtt acgaaaacg gccgattcg 
      tttatagga tccaccgaa gtcccatgt gcgaatata 
      tgatcaatt tcatacaca aactcaaac atctacccg 
      aaaagtaca tgatgaaca gcgtcttag aaaatttca 
      ccatactac aggtggtga gcaatcgag acacgcaag 
      agaccttac tgtgtacgg cgtacgtgt tcgaggtgt 
      cgacgtcag aacacggcg cccaacatc acatctaca 
      ggcttgtaa aagactagc ggaatcaag ggcgaaaaa 
      aatcccttc acttcagtg cctacaccc tcacaatac 
      tagtattat tattggtta catacgtta gaaagtagc 
      taggtaaat tacaaaacg aaattgcca tttgatgat 
      atatcatgt aaagctatc tcttgaaat ttgagttgc 
      ccttacgta aacttgagt tgagttctg cttttgtgg 
      tataaagta ctgtaggaa aagttatat attttttgt 
      tttatatat tataggttt atttctcgt cgtacttta 
      gtttaaggt tttgatatt ttatcatgt tttactcag 
      atttggaaa tatctgcac atatccgaa gtcgcagct 
      attcattga ttttggttt agtgactaa tgttgctaa 
      acacttatt tattgctag aacaacaaa atttactta 
      gttaagtta cttactttg gatacttga gataccttg 
      aatttataa agtttgcta tttgtgatg ataattttt 
      aacatttta tatagattt ttataatat ttaaaatac 
      ccagtattt taattgtaa tcattaaag tttttcgcc 
      aattaataa tttagcttt atgaggtgt cttcacaga 
      acacagtcg atgacgtga cttaaaatt aatacaatt 
      aattttagt gttgttgtg aaaacacca cggcctttg 
      tgttcaatt tggctattt tggagccaa ttagacaat 
      agaaaaact attgttaaa ctttctgag tgaactttc 
      tttcatttg catcgtgca attttgaaa aattacata 
      gaaaacgtg gtaattgaa acgatatta tgtaatttt 
      atgatacaa gtggcaatg tttttgatc tctgattta 
      atctttcgc aaattgttt gatttaagt cggcaattt 
      tggtagctt atattttgt tattatata agattaatt 
      tttacattt actcatttc tatttacat tttttcttt 
      ttcattaaa cgtaacaga cttttattg cactggcac 
      aaaagatta tcttccatg aagataaaa cttcgttca 
      ggctatgat tttttgatc attgttact taattgcta 
      gacttaact ttaacataa aaaccctag gtaagaaaa 
      aacattaca tatgaacaa taattattg taaattatc 
      atttgtact ctaatgaaa gatctattt taaaaataa 
      agtgtcagt gtaaaaagt aaaattgta tatatttct 
      aaaaaatat ggcggctat aaatatttt tatcgtctt 
      actgtaagg ttctttata aagtgtgct atatttaat 
      gaagttttt ttattcata tctggaaat gccatatca 
      tggaaatta ttgaatct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY