Status
Please wait ...
Gene information
General information
TC000324 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13029391..13031588
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000324
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09688

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000324 RA
      MDIDNGPSE GSGNGTMDI EQSLLQQFS CMGTTDKDE 
      LVQQLQKLL GSNLNYSTA AFFLDMNNW NLQAAVCSY 
      LDIESSTKL PSMALVQNP VASESESIE PNTSFDKVW 
      HMFNNGTEQ WPHGCYAQC SDGNIFNGC RVSVPALKP 
      SEDTHLTVR MTSPPQPGV YQSEWRLCT EKGAYFGDP 
      MWVIITVVE EGTMALTQQ LSSFSELGA SPPAQVAYN 
      PFVPQRSHL EQTQDATPP PGGKDMW
    CDS sequence
    • >TC000324 RA
      atggacatt gataacggc ccctctgag ggttctggg 
      aacggaaca atggatatt gaacagtca ttgttgcaa 
      caattcagt tgtatgggt acaactgat aaggacgag 
      ttggtacaa caattgcaa aagttactt gggagtaac 
      ttgaactat tcgacggct gcgtttttc ctggacatg 
      aataactgg aatcttcaa gctgctgtt tgttcctat 
      ctagacatt gaatcttcg actaaatta ccgtcaatg 
      gctcttgtc caaaaccca gtcgctagc gaatctgag 
      agtatagaa ccgaacaca agttttgac aaagtgtgg 
      cacatgttc aacaatgga acagaacag tggccccac 
      ggctgctac gctcagtgt tccgatggc aacattttc 
      aacgggtgc agagtctca gttccggcg ctgaagccc 
      tccgaagac acccatctg accgtccgg atgaccagt 
      cccccgcag ccgggcgtg tatcagagc gagtggagg 
      ctgtgcacg gaaaagggg gcctatttc ggggatccc 
      atgtgggtc ataatcacg gttgtggaa gaaggaacg 
      atggcgctc acccaacaa ctgtcaagt tttagcgag 
      ttgggggcg tcgcccccc gctcaagta gcctataat 
      ccctttgtt ccgcaacga tctcattta gaacaaact 
      caggacgcg acccccccg cccgggggc aaagacatg 
      tggtag
    mRNA sequence
    • >TC000324 RA
      cagaatctc tattttctg attggttgt ttccacatt 
      ggtaaaatg gtggtcaaa tgtcatttt tgatgaact 
      agttgtcaa atttaatgt gattttaaa aataaagtg 
      ctttgtata ggttttttc ggggttgtc attaatttt 
      taaaatgga cattgataa cggcccctc tgagggttc 
      tgggaacgg aacaatgga tattgaaca gtcattgtt 
      gcaacaatt cagttgtat gggtacaac tgataagga 
      cgagttggt acaacaatt gcaaaagtt acttgggag 
      taacttgaa ctattcgac ggctgcgtt tttcctgga 
      catgaataa ctggaatct tcaagctgc tgtttgttc 
      ctatctaga cattgaatc ttcgactaa attaccgtc 
      aatggctct tgtccaaaa cccagtcgc tagcgaatc 
      tgagagtat agaaccgaa cacaagttt tgacaaagt 
      gtggcacat gttcaacaa tggaacaga acagtggcc 
      ccacggctg ctacgctca gtgttccga tggcaacat 
      tttcaacgg gtgcagagt ctcagttcc ggcgctgaa 
      gccctccga agacaccca tctgaccgt ccggatgac 
      cagtccccc gcagccggg cgtgtatca gagcgagtg 
      gaggctgtg cacggaaaa gggggccta tttcgggga 
      tcccatgtg ggtcataat cacggttgt ggaagaagg 
      aacgatggc gctcaccca acaactgtc aagttttag 
      cgagttggg ggcgtcgcc ccccgctca agtagccta 
      taatccctt tgttccgca acgatctca tttagaaca 
      aactcagga cgcgacccc cccgcccgg gggcaaaga 
      catgtggta gtgacttta gtgccaagg aacgtaaca 
      ctatagtgt tatggtaca aataattag aaaatataa 
      acgaaattt attttgagt tttaatggt caaaacgaa 
      gctctattt tttcagatg ttggatgat attaatacg 
      gaatatttt tgtaaaaac taaattttg ttttaggtt 
      acgtgtaca gtgcattta tgtcatttt tgcggtgaa 
      aatgagcta agttcttag tacagttgt acagctaaa 
      taggcaaca agtggaata aatatactt aatttctta 
      acttaggga atgaatgga tgaattgcc gcttaaatc 
      atatttttg gccgcgata gtgcggtaa tacaacacg 
      aaaaatgta ggctttcag agactacga aaaaaaaag 
      atactttat gataataaa gtttattaa cgctgagag 
      aatattaca tttatctat ttgatattc aaaaataga 
      caatttatt tttttctga aaattttaa tcaatgcaa 
      acgcataga tccgtcacg tgttgtcaa atcgaaatt 
      tgaattaat tgaagccag agagttttg tacattatt 
      tgtgcgtta gttatttta cagacttca attttatta 
      aaaaaaaat tatatctac cgcacgtat aatgtcaat 
      tctcaaatt agaagtgat taaaatgtt aattagatg 
      taatcgaat ctaaatatt ttttgtcat tgaagagtg 
      acaagactg aaaattttc cttagtttc tttttaaca 
      atattatat tcggttttt atctacctt atttaccta 
      atcttacgt aaatccctt tttgtttcc atctgcggt 
      gtctactgt atctaactg atgctaatt tattgaaga 
      taaatgggt tttattttt gttaaattt aatgttttt 
      gttgttttt gtgtgtaca gtgtgcctt tgttttata 
      atag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY