Status
Please wait ...
Gene information
General information
TC001228 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12992029..12997723
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001228
    iBeetle screen iB_09687

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001228 RA
      MVEDSGTTL LEHKQKPIL NGTTPRKES GEPPDGGTK 
      AWLVMLGSF FCNGILFGV INSYGVLYT EFHEIFKKK 
      GSLNPSGEA ALVGSLAMG TTFLISAVA GVLTDFIGL 
      RRTTFLGGF IASSGMFLS SFCVDNKLA LYVTFGVMY 
      GLGGALAYT PSLAVLGHY FKKYLGIVN GIVTAGSSI 
      FTIAMPYVI TECLKRFKI ENTLRILAL IASLIMVFA 
      FLFKPVRET ERKEINWRN TFNVGVFRN KKYLVWATV 
      IAGSLFGYF VPYVYMLDF VKTSFPGPV DGKIPVLCI 
      GITSGIGRL IFGYIADFP KINRILLQQ VAFFIIGIL 
      TMLLPSSTA SFYWLIAIS LGMGLFDGC FIALLGPIA 
      FDICGNEGA AQAIGFLLG TCSMPLTIG PYVAGLIYE 
      KQKSYTIPL ILAGIPPTV GAVAMFLTR CIRTKNETL 
      KNGTCKVTE STDVEDGPL
    CDS sequence
    • >TC001228 RA
      atggtagaa gactccggc accaccctc ctggaacac 
      aagcagaaa cccatcctc aacggcacc accccccgg 
      aaagagagc ggcgagccc ccggatggg ggcaccaag 
      gcatggctg gtcatgctc ggctcgttc ttctgcaac 
      ggcatcctc ttcggggtg atcaactcc tacggggtg 
      ctctatacc gagttccac gagatcttc aagaagaag 
      gggtcactt aacccgtcc ggagaggca gcattggtg 
      gggtccctc gccatgggc acaacgttc ctaatctcg 
      gccgtggcc ggcgtgtta accgacttc atcggcctc 
      cgcagaacg accttcctg ggcggcttc atcgcctcc 
      agcggcatg ttcctatcg tcgttttgc gtcgacaat 
      aaactagcc ctttacgtg actttcggc gtaatgtac 
      ggcctgggg ggcgcctta gcctacacc ccctccctg 
      gcagtcctc ggccactac ttcaagaaa tacctcggc 
      atagtcaac ggtattgta actgcgggc agttcaatc 
      ttcaccatt gccatgcca tacgtcata accgagtgt 
      ctcaaacgc ttcaaaatc gaaaacacg ctccgaatt 
      ctcgcccta attgccagc ctcatcatg gttttcgcg 
      ttcctcttc aaaccggtg cgcgaaacc gagcgcaag 
      gaaatcaac tggaggaac accttcaac gttggcgtg 
      ttccgcaac aagaaatac ctagtctgg gcgacggtt 
      atagccggt tccctgttc ggttacttc gttccctac 
      gtctacatg ctcgatttc gtcaaaacc agcttcccg 
      ggcccggtc gacgggaaa atcccggtc ctgtgcatc 
      gggataacg tccgggatc gggcgactc atcttcggc 
      tacatcgcc gatttcccc aaaatcaac cgaatcctc 
      cttcagcaa gtcgctttc ttcattatc ggcatcctc 
      accatgctc ttgccctcg tccacagcc tcgttctac 
      tggctgatt gccatcagt ctgggcatg ggcctgttc 
      gatgggtgc tttatcgcc ctgctgggc cccatcgcg 
      ttcgacatc tgcggcaac gagggcgct gcccaggcc 
      atcggcttc ctgctgggc acctgctcg atgccgctc 
      accatcggg ccctacgtc gctgggctc atctacgag 
      aagcagaaa agctacacc attcccctg attctggcc 
      gggattccg cccactgtg ggcgctgtg gccatgttc 
      ctaacgagg tgtatacgg acgaaaaac gaaacgctc 
      aaaaacggc acctgtaaa gtcacagaa tccacggac 
      gtcgaagat ggtcctcta tga
    mRNA sequence
    • >TC001228 RA
      tctttatca actccgcga gcgcaagcg ccggctatt 
      cactctcta cactaggtc agatcagag cttagccgc 
      actcagtcg atgtcgtcc acgtgagag ccggcaatg 
      tgtcaagtg cctcgacaa tggtagaag actccggca 
      ccaccctcc tggaacaca agcagaaac ccatcctca 
      acggcacca ccccccgga aagagagcg gcgagcccc 
      cggatgggg gcaccaagg catggctgg tcatgctcg 
      gctcgttct tctgcaacg gcatcctct tcggggtga 
      tcaactcct acggggtgc tctataccg agttccacg 
      agatcttca agaagaagg ggtcactta acccgtccg 
      gagaggcag cattggtgg ggtccctcg ccatgggca 
      caacgttcc taatctcgg ccgtggccg gcgtgttaa 
      ccgacttca tcggcctcc gcagaacga ccttcctgg 
      gcggcttca tcgcctcca gcggcatgt tcctatcgt 
      cgttttgcg tcgacaata aactagccc tttacgtga 
      ctttcggcg taatgtacg gcctggggg gcgccttag 
      cctacaccc cctccctgg cagtcctcg gccactact 
      tcaagaaat acctcggca tagtcaacg gtattgtaa 
      ctgcgggca gttcaatct tcaccattg ccatgccat 
      acgtcataa ccgagtgtc tcaaacgct tcaaaatcg 
      aaaacacgc tccgaattc tcgccctaa ttgccagcc 
      tcatcatgg ttttcgcgt tcctcttca aaccggtgc 
      gcgaaaccg agcgcaagg aaatcaact ggaggaaca 
      ccttcaacg ttggcgtgt tccgcaaca agaaatacc 
      tagtctggg cgacggtta tagccggtt ccctgttcg 
      gttacttcg ttccctacg tctacatgc tcgatttcg 
      tcaaaacca gcttcccgg gcccggtcg acgggaaaa 
      tcccggtcc tgtgcatcg ggataacgt ccgggatcg 
      ggcgactca tcttcggct acatcgccg atttcccca 
      aaatcaacc gaatcctcc ttcagcaag tcgctttct 
      tcattatcg gcatcctca ccatgctct tgccctcgt 
      ccacagcct cgttctact ggctgattg ccatcagtc 
      tgggcatgg gcctgttcg atgggtgct ttatcgccc 
      tgctgggcc ccatcgcgt tcgacatct gcggcaacg 
      agggcgctg cccaggcca tcggcttcc tgctgggca 
      cctgctcga tgccgctca ccatcgggc cctacgtcg 
      ctgggctca tctacgaga agcagaaaa gctacacca 
      ttcccctga ttctggccg ggattccgc ccactgtgg 
      gcgctgtgg ccatgttcc taacgaggt gtatacgga 
      cgaaaaacg aaacgctca aaaacggca cctgtaaag 
      tcacagaat ccacggacg tcgaagatg gtcctctat 
      gatttccca aacatgact cgactagtt tacacattt 
      agacaaggt ttttgcttt catttattc tgctagcta 
      tatcgtgtc tttaattta atctatttt ttaatgtat 
      tactgcatt tattttata gttttatac gatttcata 
      cgattaggc ggttaacaa ttttgacaa taaatatac 
      aatgtacaa aaagaatgt tgtttgtgg cggacgaca 
      tggtttgtt aacttatta catatcaat gcaaaagtt 
      atattttat cgaaggact gtacgaaat tgttaataa 
      aagtatttt aacgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY