Status
Please wait ...
Gene information
General information
TC001220 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12951979..12953853
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001220
    iBeetle screen iB_09685

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001220 RA
      MASDRKILS LRFNQDQGC FSCCMESGV RIYNVEPLV 
      EKSHYDVET VGSVSKCEM LYRTNILAL VSGGTRPMF 
      SDNILLIFD DLLKKFILE ITFPSSIQA VRLRKDKII 
      VALLTSIHV FSFPSPTQR LFSLETIKN PRGLLELSP 
      LGAGEKQII IFPGHKTGS VQILDLACT EIGISSAPV 
      WISAHKGEL ACLAFNQQA TKIATASVQ GTLIRVWDL 
      ATKTQLVEL RRGTDPATI YCINFSSNS DYLCCSSDK 
      GTVHIFAIK DTSRNKRMI IANTNLFGK YANSQWALA 
      NFTVPQECA CICAFTENN SVIIAACLD GTFHKYVFN 
      QDGNCNRDG FDVFLDVSD DVENLL
    CDS sequence
    • >TC001220 RA
      atggccagt gatcggaaa atcctaagt ttgaggttc 
      aaccaggac caaggttgt ttctcgtgt tgtatggaa 
      agtggcgta agaatttac aatgtggaa ccactggtt 
      gagaaaagc cactacgac gttgaaacg gtcggaagt 
      gtctcaaaa tgtgagatg ttgtaccgc accaacata 
      ctcgcacta gtctcgggg ggcacccgc cccatgttc 
      tccgacaac atcctcctc attttcgac gacttactc 
      aaaaaattc atcctggaa ataactttc ccctcgtcc 
      atccaagct gtgcgttta cgcaaagac aaaataatc 
      gtagctcta ttgacaagc atccacgtg ttctccttc 
      ccctcgcca acgcagcgc ctcttctcc ctggaaacc 
      atcaaaaac cccagaggc ttgctcgag ttgagccct 
      ctgggcgca ggcgagaaa caaatcatc attttcccc 
      ggacataaa acgggaagc gtgcaaatt ctggacctg 
      gcctgcact gaaattggc atttccagt gccccagtc 
      tggatcagc gcccacaaa ggcgaactc gcctgcttg 
      gctttcaac caacaagcc accaaaatc gcaacggcc 
      tcggtccaa ggcaccctg atcagggtc tgggacttg 
      gcgactaaa acgcaattg gtggagctg aggaggggc 
      acagacccg gccaccatt tattgtatc aattttagc 
      agcaactcg gattatttg tgctgttcg agcgataaa 
      ggcactgtg catattttc gcaatcaaa gacacgtcc 
      cgtaacaag cgaatgata attgcaaat acgaatctt 
      ttcgggaaa tacgccaac tcgcagtgg gctttggcc 
      aattttacc gtcccgcaa gagtgtgcg tgtatttgt 
      gcctttaca gaaaataat tctgtaatt attgctgct 
      tgtctcgac ggcacgttt cataagtac gtcttcaac 
      caagacggc aattgcaac agagacgga ttcgatgtt 
      tttctcgac gtttcagac gacgtcgaa aatttactt 
      taa
    mRNA sequence
    • >TC001220 RA
      aatgtcaac aaccaaaat aaaacaaaa gaacagctg 
      aaaagctgt ctcgatttc gactttcga ttaaaattc 
      cgtcttttc gtgatttaa ttaaattag gttaagttt 
      tgtgattct agtcatact gtaaggact aaagaatcc 
      ttatctagt gttaaattc agttcctaa gaaatatct 
      ttattttcc acgtgtttc atggttcgc agtttctgt 
      ttactggtg tttcttatc gttcagctc aacaaaaac 
      gtgtacaca taaggagaa caaacagtc attgttgat 
      aattaggaa ttattattt atttaaaac ggtgttgac 
      acccattac aatcattta attgtatgt tgatgattg 
      tcattttca tacatttgg aagatgttt aaaaattgc 
      aacgcaatt gctttttgt gactgatta tttggagtc 
      cttccacta gtgaaaaat ggccagtga tcggaaaat 
      cctaagttt gaggttcaa ccaggacca aggttgttt 
      ctcgtgttg tatggaaag tggcgtaag aatttacaa 
      tgtggaacc actggttga gaaaagcca ctacgacgt 
      tgaaacggt cggaagtgt ctcaaaatg tgagatgtt 
      gtaccgcac caacatact cgcactagt ctcgggggg 
      cacccgccc catgttctc cgacaacat cctcctcat 
      tttcgacga cttactcaa aaaattcat cctggaaat 
      aactttccc ctcgtccat ccaagctgt gcgtttacg 
      caaagacaa aataatcgt agctctatt gacaagcat 
      ccacgtgtt ctccttccc ctcgccaac gcagcgcct 
      cttctccct ggaaaccat caaaaaccc cagaggctt 
      gctcgagtt gagccctct gggcgcagg cgagaaaca 
      aatcatcat tttccccgg acataaaac gggaagcgt 
      gcaaattct ggacctggc ctgcactga aattggcat 
      ttccagtgc cccagtctg gatcagcgc ccacaaagg 
      cgaactcgc ctgcttggc tttcaacca acaagccac 
      caaaatcgc aacggcctc ggtccaagg caccctgat 
      cagggtctg ggacttggc gactaaaac gcaattggt 
      ggagctgag gaggggcac agacccggc caccattta 
      ttgtatcaa ttttagcag caactcgga ttatttgtg 
      ctgttcgag cgataaagg cactgtgca tattttcgc 
      aatcaaaga cacgtcccg taacaagcg aatgataat 
      tgcaaatac gaatctttt cgggaaata cgccaactc 
      gcagtgggc tttggccaa ttttaccgt cccgcaaga 
      gtgtgcgtg tatttgtgc ctttacaga aaataattc 
      tgtaattat tgctgcttg tctcgacgg cacgtttca 
      taagtacgt cttcaacca agacggcaa ttgcaacag 
      agacggatt cgatgtttt tctcgacgt ttcagacga 
      cgtcgaaaa tttacttta aagtggcat gacaactta 
      atttttgag ctattttct cacttttat attcacaca 
      tacatgtcg taaacagtg gaaacctct tattaattt 
      attgaacaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY