Status
Please wait ...
Gene information
General information
TC001186 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12343878..12352472
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001186
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09680

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001186 RA
      MRLVVTQLM ALWLGLSMA FPGTGRRNT HDMDTPIYT 
      NNIQRFVHR QENKNEEIR ALKRVFLSN RSVTCNDGS 
      QAGFYLRKS YTSKKWIIF LEGGWYCYD HHSCRNRWL 
      KQRHYMTST GWPDARDIG GILSGSMEE NPFWWNANH 
      VFIPYCTSD SWSGSKPHS RSETFSFMG SILVQQVVQ 
      DLLTLGLEN STDLLLTGS SAGGTGVML NLDPVREFL 
      HDKKGLRHI VVKGVTDSG WFLDRTPYA PTLKPAVDA 
      IRRGIDLWG GKVPHRCKE LYPDEPWRC YFGYRLYPT 
      LKTELFVFQ WLFDEAQMD ADNVGAPVT KQQWDYIHK 
      MGDALRQSF ENVSAVFAP SCISHSVLT KRDWQNVKI 
      DDISIPEAL HCWEQKLHR RRIKKSRNT KMFADQPKP 
      LRRQTLNST LTDEVGQKK KRRRKNRKS RKGKKKKDA 
      VQASGNRTS ELNLMGRPQ RSVLHTQRR KHCTHRRLE 
      RCSWPQCNH SCPRLHNPF TGEEMDFIE LLKSFGLDM 
      ESVAKALGI DMQTLNNMD HSELLNLLT QQSN
    CDS sequence
    • >TC001186 RA
      atgcgctta gtcgtcaca cagttgatg gcgttatgg 
      ttgggtctg tccatggct tttcccggc accggccgc 
      aggaacacg cacgacatg gacactcct atctacacc 
      aacaacatc caaagattc gtccatcgg caggagaac 
      aaaaacgag gaaattcgg gctttgaag cgcgttttc 
      ctgtcgaac agatccgtc acttgtaac gacggatcg 
      caggccgga ttttaccta agaaagtct tacacgagt 
      aagaaatgg attatcttt ttagaaggc ggatggtat 
      tgctatgac caccatagt tgcagaaac agatggctg 
      aaacagcgt cattacatg acttcgaca ggatggccc 
      gacgccagg gacataggg ggaattctc tccggcagc 
      atggaggaa aaccctttc tggtggaac gccaaccac 
      gtgttcatc ccttactgt accagcgat tcctggagc 
      ggctcaaaa ccccactcc cggagcgag acgttcagc 
      ttcatgggc tcaatcctc gtgcaacaa gtcgtccaa 
      gatttacta actttggga cttgaaaat agcaccgat 
      ttgctttta actgggagc agcgctgga ggaactgga 
      gttatgtta aacttggac cctgttcgt gagtttctc 
      cacgacaag aaaggtctg agacacatc gttgtgaaa 
      ggagtcacc gactcgggc tggtttctc gatcgaact 
      ccctatgcc ccgactctg aagcctgca gttgacgcg 
      atccggaga gggatcgac ctgtggggc gggaaagtc 
      ccccacagg tgcaaggag ttgtatccg gacgagcct 
      tggcggtgt tactttggg tacaggttg taccccact 
      ttgaaaacc gagctcttt gtctttcaa tggctgttt 
      gatgaggcg caaatggac gcagataac gtcggtgct 
      cccgtaaca aaacaacag tgggattac attcataaa 
      atgggggac gcattgcgg cagagtttc gagaacgtt 
      tccgccgtg ttcgccccc tcgtgcatc tcgcactcg 
      gttttgacc aagcgcgat tggcagaac gtgaaaatc 
      gacgatatt tcaatccct gaagcgctc cattgttgg 
      gagcaaaaa ctgcacagg aggcggatc aagaaatcc 
      cgcaacacc aaaatgttc gccgaccag cccaagcca 
      ctccggcga caaactctc aattcgaca ctcacggac 
      gaagtcgga caaaagaaa aagcgccgg aggaaaaac 
      cgcaagagc cggaaagga aagaaaaag aaagatgcg 
      gtccaggcg agtgggaac aggacatct gagctgaat 
      ttaatgggg aggccccag cgctcggtc ctgcacaca 
      caaaggagg aaacattgc actcatcgg agactggag 
      cggtgttcg tggccgcag tgtaatcac tcgtgtccg 
      cgactgcac aaccccttc acgggggag gaaatggac 
      tttatcgaa ctgctcaaa tcgttcgga cttgacatg 
      gagagcgtg gccaaggca ctaggcatt gacatgcaa 
      acgctgaac aacatggac cattcagaa cttttaaat 
      ttattgacg cagcaatcg aactga
    mRNA sequence
    • >TC001186 RA
      ttggtcaac aattcctcc tccggttat ctgtagata 
      tcaacatat tacgaatgt gtacaagca aataaatag 
      cctgcgcca gaggagttt tgccgacag aggatgccg 
      ccgcgtcat ttccatttc tcgtgcgta attcacgaa 
      aattacgtg gaacgtgcg acgctggaa tgcaggctc 
      gcctcagga ggcttgctg ggggttgcc ggtggtaat 
      tagtggtgc gcacccccg atcgggggt ggtggggcc 
      gctatatag aagcgatcc aagaagtcg acgttagtc 
      cgaagcaaa gtgctggtg tgtgagctg gtgaccgtt 
      agcaggtga agaacacgt gcagacatg cgcttagtc 
      gtcacacag ttgatggcg ttatggttg ggtctgtcc 
      atggctttt cccggcacc ggccgcagg aacacgcac 
      gacatggac actcctatc tacaccaac aacatccaa 
      agattcgtc catcggcag gagaacaaa aacgaggaa 
      attcgggct ttgaagcgc gttttcctg tcgaacaga 
      tccgtcact tgtaacgac ggatcgcag gccggattt 
      tacctaaga aagtcttac acgagtaag aaatggatt 
      atcttttta gaaggcgga tggtattgc tatgaccac 
      catagttgc agaaacaga tggctgaaa cagcgtcat 
      tacatgact tcgacagga tggcccgac gccagggac 
      ataggggga attctctcc ggcagcatg gaggaaaac 
      cctttctgg tggaacgcc aaccacgtg ttcatccct 
      tactgtacc agcgattcc tggagcggc tcaaaaccc 
      cactcccgg agcgagacg ttcagcttc atgggctca 
      atcctcgtg caacaagtc gtccaagat ttactaact 
      ttgggactt gaaaatagc accgatttg cttttaact 
      gggagcagc gctggagga actggagtt atgttaaac 
      ttggaccct gttcgtgag tttctccac gacaagaaa 
      ggtctgaga cacatcgtt gtgaaagga gtcaccgac 
      tcgggctgg tttctcgat cgaactccc tatgccccg 
      actctgaag cctgcagtt gacgcgatc cggagaggg 
      atcgacctg tggggcggg aaagtcccc cacaggtgc 
      aaggagttg tatccggac gagccttgg cggtgttac 
      tttgggtac aggttgtac cccactttg aaaaccgag 
      ctctttgtc tttcaatgg ctgtttgat gaggcgcaa 
      atggacgca gataacgtc ggtgctccc gtaacaaaa 
      caacagtgg gattacatt cataaaatg ggggacgca 
      ttgcggcag agtttcgag aacgtttcc gccgtgttc 
      gccccctcg tgcatctcg cactcggtt ttgaccaag 
      cgcgattgg cagaacgtg aaaatcgac gatatttca 
      atccctgaa gcgctccat tgttgggag caaaaactg 
      cacaggagg cggatcaag aaatcccgc aacaccaaa 
      atgttcgcc gaccagccc aagccactc cggcgacaa 
      actctcaat tcgacactc acggacgaa gtcggacaa 
      aagaaaaag cgccggagg aaaaaccgc aagagccgg 
      aaaggaaag aaaaagaaa gatgcggtc caggcgagt 
      gggaacagg acatctgag ctgaattta atggggagg 
      ccccagcgc tcggtcctg cacacacaa aggaggaaa 
      cattgcact catcggaga ctggagcgg tgttcgtgg 
      ccgcagtgt aatcactcg tgtccgcga ctgcacaac 
      cccttcacg ggggaggaa atggacttt atcgaactg 
      ctcaaatcg ttcggactt gacatggag agcgtggcc 
      aaggcacta ggcattgac atgcaaacg ctgaacaac 
      atggaccat tcagaactt ttaaattta ttgacgcag 
      caatcgaac tgattacat tcgctcgga acggcccag 
      tgaagatgg atgctttaa ttttttcat tcaacttgt 
      acataatga atatttatt gtagagaac tattttttt 
      atcgtagtt aaggctaga ctaggtgtt tgtaagggg 
      ggcaagcct ctcaactcc aaagaacgc ttgcccgga 
      atttgtaac aggactgat ttttgttct acctcaact 
      gtacagagt ttagtcagt aagttacct tatttttgt 
      acgataggt gttacaata aagaaagag taccgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY