Status
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Gene information
General information
TC000383 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12228375..12230185
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000383
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09674

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000383 RA
      MVLFEQIRL KNCRLCHTK PGQHDIKTD KKLVKEIYE 
      CTKVKVNDK SMELLPRSV CEECKKTIK CFSLYKQHI 
      RKVDQCFRR ILKNCEAKS KDTKEEIFK CEQCPGEFS 
      DHDLFKNHL NSHQISVSQ EEIKAEVSC PKCDNKFCN 
      LEEYGSHNC FTEEENSQH DSFENKIET EDLEAFKKA 
      KFVCSWKNC KKILSSAYT LKTHELKHQ GKRSFLCVT 
      CGKGFITKN SLTCHEKIH LEVKGYVCK LCNLGFSVR 
      SNLRAHEKK HHEGVRFYC SQCSRAFAS KCSLQRHER 
      IHTGIKEFK CQSCPSAFY TKKELLKHQ RYHQGLRLH 
      KCDECFKTF FERHHLIIH LRSHTGERP YVCQFPNCG 
      KSFTESQKL KRHHNAKHA QT
    • >TC000383 RB
      MVLFEQIRL KNCRLCHTK PGQHDIKTD KKLVKEIYE 
      CTKVKVNDK SMELLPRSV CEECKKTIK CFSLYKQHI 
      RKVDQCFRR ILKNCEAKS KDTKEEIFK CEQCPGEFS 
      DHDLFKNHL NSHQISVSQ EEIKAEVSC PKCDNKFCN 
      LEEYGSHNC FTEEENSQH DSFENKIET EDLEAFKKA 
      KFVCSWKNC KKILSSAYT LKTHELKHQ GKRSFLCVT 
      CGKGFITKN SLTCHEKIH LEVKGYVCK LCNLGFSVR 
      SNLRAHEKK HHEGVRFYC SQCSRAFAS KCSLQRHER 
      IHTGIKEFK CQSCPSAFY TKKELLKHQ RYHQVGIFV 
      I
    CDS sequence
    • >TC000383 RA
      atggtttta ttcgaacaa ataaggctg aaaaactgc 
      cgattgtgt cacacaaag cccggccaa cacgacata 
      aaaacagac aaaaaatta gtaaaagaa atttacgaa 
      tgcacaaaa gtgaaggta aatgataaa tctatggag 
      cttttgccg cgttctgtg tgtgaggag tgcaaaaaa 
      accataaaa tgtttttcg ctttacaag caacacatt 
      cgcaaagtg gatcagtgt ttcagaagg attttaaaa 
      aattgcgaa gccaaaagt aaggacact aaagaagaa 
      atcttcaag tgtgaacaa tgtccaggg gagttcagt 
      gatcatgat ttatttaag aatcattta aattcacac 
      cagataagt gttagtcag gaggaaatt aaagcagaa 
      gtttcgtgt ccgaaatgt gataacaag ttttgtaac 
      ttggaggag tatgggtct cacaactgt ttcacagaa 
      gaagaaaat tcgcaacat gatagtttt gaaaataaa 
      atagaaaca gaggatttg gaggcgttt aaaaaagcc 
      aaattcgtt tgtagttgg aagaattgt aaaaaaatc 
      ctcagttcc gcctacaca ttaaaaacg catgagctc 
      aaacatcag gggaaacgc tcgtttttg tgtgtaact 
      tgtggcaaa ggcttcatc acaaaaaat tcattaacc 
      tgccacgaa aaaatacac ctggaagtg aaaggttac 
      gtgtgtaag ctctgtaat cttgggttt tctgtgcga 
      agtaatctg agggcacat gaaaaaaag catcatgaa 
      ggagtcaga ttttactgc tcgcagtgt tccagagca 
      tttgcttca aagtgcagt ttgcagagg cacgaaaga 
      attcacaca ggaatcaag gaattcaag tgccagtcc 
      tgcccttcg gcgttctat accaaaaaa gagctcctg 
      aagcaccaa aggtaccat cagggtttg cgcttacat 
      aagtgtgac gaatgcttt aaaacattt ttcgaacgg 
      caccacctg atcatccat ttgaggagc cacactggg 
      gagaggcct tatgtatgc cagtttccc aattgtggc 
      aagtctttc acggaaagt caaaaacta aagaggcat 
      cataatgcc aaacatgcc caaacataa
    • >TC000383 RB
      atggtttta ttcgaacaa ataaggctg aaaaactgc 
      cgattgtgt cacacaaag cccggccaa cacgacata 
      aaaacagac aaaaaatta gtaaaagaa atttacgaa 
      tgcacaaaa gtgaaggta aatgataaa tctatggag 
      cttttgccg cgttctgtg tgtgaggag tgcaaaaaa 
      accataaaa tgtttttcg ctttacaag caacacatt 
      cgcaaagtg gatcagtgt ttcagaagg attttaaaa 
      aattgcgaa gccaaaagt aaggacact aaagaagaa 
      atcttcaag tgtgaacaa tgtccaggg gagttcagt 
      gatcatgat ttatttaag aatcattta aattcacac 
      cagataagt gttagtcag gaggaaatt aaagcagaa 
      gtttcgtgt ccgaaatgt gataacaag ttttgtaac 
      ttggaggag tatgggtct cacaactgt ttcacagaa 
      gaagaaaat tcgcaacat gatagtttt gaaaataaa 
      atagaaaca gaggatttg gaggcgttt aaaaaagcc 
      aaattcgtt tgtagttgg aagaattgt aaaaaaatc 
      ctcagttcc gcctacaca ttaaaaacg catgagctc 
      aaacatcag gggaaacgc tcgtttttg tgtgtaact 
      tgtggcaaa ggcttcatc acaaaaaat tcattaacc 
      tgccacgaa aaaatacac ctggaagtg aaaggttac 
      gtgtgtaag ctctgtaat cttgggttt tctgtgcga 
      agtaatctg agggcacat gaaaaaaag catcatgaa 
      ggagtcaga ttttactgc tcgcagtgt tccagagca 
      tttgcttca aagtgcagt ttgcagagg cacgaaaga 
      attcacaca ggaatcaag gaattcaag tgccagtcc 
      tgcccttcg gcgttctat accaaaaaa gagctcctg 
      aagcaccaa aggtaccat caggtgggt atttttgta 
      atttag
    mRNA sequence
    • >TC000383 RA
      aaaagtaag gttagggta cggtttacc aacaaaaac 
      gaaaatggt tttattcga acaaataag gctgaaaaa 
      ctgccgatt gtgtcacac aaagcccgg ccaacacga 
      cataaaaac agacaaaaa attagtaaa agaaattta 
      cgaatgcac aaaagtgaa ggtaaatga taaatctat 
      ggagctttt gccgcgttc tgtgtgtga ggagtgcaa 
      aaaaaccat aaaatgttt ttcgcttta caagcaaca 
      cattcgcaa agtggatca gtgtttcag aaggatttt 
      aaaaaattg cgaagccaa aagtaagga cactaaaga 
      agaaatctt caagtgtga acaatgtcc aggggagtt 
      cagtgatca tgatttatt taagaatca tttaaattc 
      acaccagat aagtgttag tcaggagga aattaaagc 
      agaagtttc gtgtccgaa atgtgataa caagttttg 
      taacttgga ggagtatgg gtctcacaa ctgtttcac 
      agaagaaga aaattcgca acatgatag ttttgaaaa 
      taaaataga aacagagga tttggaggc gtttaaaaa 
      agccaaatt cgtttgtag ttggaagaa ttgtaaaaa 
      aatcctcag ttccgccta cacattaaa aacgcatga 
      gctcaaaca tcaggggaa acgctcgtt tttgtgtgt 
      aacttgtgg caaaggctt catcacaaa aaattcatt 
      aacctgcca cgaaaaaat acacctgga agtgaaagg 
      ttacgtgtg taagctctg taatcttgg gttttctgt 
      gcgaagtaa tctgagggc acatgaaaa aaagcatca 
      tgaaggagt cagatttta ctgctcgca gtgttccag 
      agcatttgc ttcaaagtg cagtttgca gaggcacga 
      aagaattca cacaggaat caaggaatt caagtgcca 
      gtcctgccc ttcggcgtt ctataccaa aaaagagct 
      cctgaagca ccaaaggta ccatcaggg tttgcgctt 
      acataagtg tgacgaatg ctttaaaac atttttcga 
      acggcacca cctgatcat ccatttgag gagccacac 
      tggggagag gccttatgt atgccagtt tcccaattg 
      tggcaagtc tttcacgga aagtcaaaa actaaagag 
      gcatcataa tgccaaaca tgcccaaac ataattaat 
      tgtatttcc ttttattaa taaaattgt aaaacagaa
    • >TC000383 RB
      aaaagtaag gttagggta cggtttacc aacaaaaac 
      gaaaatggt tttattcga acaaataag gctgaaaaa 
      ctgccgatt gtgtcacac aaagcccgg ccaacacga 
      cataaaaac agacaaaaa attagtaaa agaaattta 
      cgaatgcac aaaagtgaa ggtaaatga taaatctat 
      ggagctttt gccgcgttc tgtgtgtga ggagtgcaa 
      aaaaaccat aaaatgttt ttcgcttta caagcaaca 
      cattcgcaa agtggatca gtgtttcag aaggatttt 
      aaaaaattg cgaagccaa aagtaagga cactaaaga 
      agaaatctt caagtgtga acaatgtcc aggggagtt 
      cagtgatca tgatttatt taagaatca tttaaattc 
      acaccagat aagtgttag tcaggagga aattaaagc 
      agaagtttc gtgtccgaa atgtgataa caagttttg 
      taacttgga ggagtatgg gtctcacaa ctgtttcac 
      agaagaaga aaattcgca acatgatag ttttgaaaa 
      taaaataga aacagagga tttggaggc gtttaaaaa 
      agccaaatt cgtttgtag ttggaagaa ttgtaaaaa 
      aatcctcag ttccgccta cacattaaa aacgcatga 
      gctcaaaca tcaggggaa acgctcgtt tttgtgtgt 
      aacttgtgg caaaggctt catcacaaa aaattcatt 
      aacctgcca cgaaaaaat acacctgga agtgaaagg 
      ttacgtgtg taagctctg taatcttgg gttttctgt 
      gcgaagtaa tctgagggc acatgaaaa aaagcatca 
      tgaaggagt cagatttta ctgctcgca gtgttccag 
      agcatttgc ttcaaagtg cagtttgca gaggcacga 
      aagaattca cacaggaat caaggaatt caagtgcca 
      gtcctgccc ttcggcgtt ctataccaa aaaagagct 
      cctgaagca ccaaaggta ccatcaggt gggtatttt 
      tgtaattta gcgtgttct tgttggtgg cactgttac 
      taacgcgtc aaacgtcat cgcaaatat tgaggttac 
      aattaggat aataaataa cgcccttgg ttgataact 
      gccaccatg tcgagttct attgccgac gagaacgag 
      aacaccgag cgcctcgac ggcgaggga gccaagagc 
      gagaaaatg ttcactttg aaaaaatgg aacgctgtt 
      gcaatgtgg agctgggac gtggaatgc gacacttgt 
      gcgatttgt cgcgtgcaa gtgatggac gcgtgtttg 
      cggtgccaa gctgagagc aaaaaagac tcgtacggg 
      aagcaggat tgcgtggtg gtgtggggg ggagtgcaa 
      ccactcgtt tcactattg ttgcatgtc tttgtgggt 
      caagcaaaa taatcgctg cccgttgtg tcagcaaga 
      atggtccat ccagaggat gggcaaata atgatttcg 
      tgtactttt cagggtttg cgcttacat aagtgtgac 
      gaatgcttt aaaacattt ttcgaacgg caccacctg 
      atcatccat ttgaggagc cacactggg gagaggcct 
      tatgtatgc cagtttccc aattgtggc aagtctttc 
      acggaaagt caaaaacta aagaggcat cataatgcc 
      aaacatgcc caaacataa ttaattgta tttcctttt 
      attaataaa attgtaaaa cagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY