Status
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Gene information
General information
TC032194
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000389,TC000390
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12182510..12184657
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032194
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09672

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032194 RA
      MRVCRLCLH QEPENLISV LGNKDVMEK IKLYVSIEI 
      TDNDDLPTT ACNSCVEKI NDWSVFIDN CTKTNEVLL 
      NLIQKGQFL NESRSQHDF KYLLQDEKR ELFEDELNF 
      VPENVYICE ICSEQFTNC LEHLDHQHA HDGHPVFKC 
      DKCSEVYST RDALVQHDR NHKRACPKC GKMILKSSM 
      KQAHRPPHV IPPIPPTQN NRVIPRCSQ CHSCFNSRA 
      ALQQHVITI HTDRKDHIC ETCGKRFSS KTAMTVHAK 
      SHSDERLYN CKLCDYKGR TASAVYVHM STHAREVCV 
      CEVCSKIFK SNRNLNDHL RRVHSKEKK HKCTYCDKK 
      FVDRYMLTV HVRCHTGVR PYACHLCEK AFIRSDGLK 
      EHLATHGER VWFKCDNGK RKSGKDYIC EALKANLGD 
      NNCTIIRIS GPLKRLYAE SHDLTTGDV NEMMTDGPL 
      KEKFRAEMI QWSDEIRGR DFGFFCKAA TDLADLKPF 
      WIVSDIRRK TDIHWFKNT YKDKIIKLI RIQADEVTR 
      QKRGWVFTE GVDDVTSEC DLDDFSDWD LIIKNNNAE 
      ELGEGLGKL MQFLN
    CDS sequence
    • >TC032194 RA
      atgcgagtc tgtcggtta tgtttgcac caagaaccg 
      gaaaatttg atttcagtg ttaggcaat aaagatgta 
      atggaaaaa attaagctc tatgtttca attgagatc 
      acagataac gatgacttg cccacaacg gcgtgcaat 
      tcttgcgtt gaaaaaata aacgattgg tctgttttt 
      attgataat tgtaccaaa acgaatgag gtactgttg 
      aatttaata caaaagggg caattcttg aatgaaagt 
      cgcagtcag catgacttt aaatacctc ctacaggat 
      gaaaaacgg gaattattt gaagacgag cttaatttc 
      gtccctgaa aacgtgtac atttgcgag atatgttca 
      gagcagttc accaactgt ttggaacac ctggaccac 
      cagcacgcc cacgacggg caccctgta tttaaatgt 
      gataaatgt tccgaagtg tattccaca agagacgcc 
      ttggtccaa cacgacaga aaccacaaa cgcgcgtgc 
      cccaagtgc ggcaaaatg atcctcaaa agcagcatg 
      aagcaagca caccgaccg ccacatgta attccccca 
      attcccccc acccaaaat aaccgtgtg attcccagg 
      tgttcgcaa tgccacagt tgcttcaat tcgcgagca 
      gccctccaa cagcacgtc atcacgata cacacggac 
      cgaaaagac cacatttgc gagacctgc ggcaaacgt 
      ttctcctcc aaaaccgca atgactgtt cacgcaaaa 
      tcacacagt gatgaacgg ttgtacaat tgcaaattg 
      tgcgattac aagggccgg acggcctca gccgtgtac 
      gtccacatg tccacacac gcccgcgag gtgtgcgtg 
      tgcgaggtc tgctcgaaa atattcaaa agcaaccgg 
      aacttgaac gaccatctc aggcgcgtt cacagcaaa 
      gaaaagaag cacaagtgc acatattgc gacaagaag 
      ttcgttgat aggtacatg ctgacggtg catgtgagg 
      tgtcacacg ggcgtgcgc ccctatgcc tgccacttg 
      tgcgagaaa gcgttcatt cggtcggac gggctgaag 
      gaacatttg gcaacgcat ggggagcga gtctggttt 
      aagtgcgat aatgggaaa aggaagtca gggaaagac 
      tacatttgc gaagcgttg aaagcaaat ctgggggac 
      aataattgc acaataatt aggatttca gggccgttg 
      aaacgattg tatgctgaa agtcatgat ttaacgact 
      ggtgatgta aatgagatg atgactgat ggtccgctt 
      aaagaaaaa tttcgcgcg gaaatgatc caatggagc 
      gatgaaatc agagggaga gattttggg tttttttgc 
      aaagcggcc acagatttg gccgattta aagccgttt 
      tggatcgtt agcgacatt agacgcaaa actgatatt 
      cactggttc aaaaacacg tacaaagat aagattatt 
      aaattaatt cgaattcaa gcggatgaa gtgactagg 
      caaaaaagg ggatgggtc tttactgag ggagttgat 
      gatgttacg tctgagtgt gacttagac gatttcagc 
      gattgggac ttaattatt aaaaacaat aatgcagaa 
      gagttggga gagggattg ggcaaatta atgcaattt 
      ctcaattaa
    mRNA sequence
    • >TC032194 RA
      atgacctaa ccctatttt ttatgcctt tttcgaacg 
      ttttttgtg acaatattt acgtaataa tgcgagtct 
      gtcggttat gtttgcacc aagaaccgg aaaatttga 
      tttcagtgt taggcaata aagatgtaa tggaaaaaa 
      ttaagctct atgtttcaa ttgagatca cagataacg 
      atgacttgc ccacaacgg cgtgcaatt cttgcgttg 
      aaaaaataa acgattggt ctgttttta ttgataatt 
      gtaccaaaa cgaatgagg tactgttga atttaatac 
      aaaaggggc aattcttga atgaaagtc gcagtcagc 
      atgacttta aatacctcc tacaggatg aaaaacggg 
      aattatttg aagacgagc ttaatttcg tccctgaaa 
      acgtgtaca tttgcgaga tatgttcag agcagttca 
      ccaactgtt tggaacacc tggaccacc agcacgccc 
      acgacgggc accctgtat ttaaatgtg ataaatgtt 
      ccgaagtgt attccacaa gagacgcct tggtccaac 
      acgacagaa accacaaac gcgcgtgcc ccaagtgcg 
      gcaaaatga tcctcaaaa gcagcatga agcaagcac 
      accgaccgc cacatgtaa ttcccccaa ttcccccca 
      cccaaaata accgtgtga ttcccaggt gttcgcaat 
      gccacagtt gcttcaatt cgcgagcag ccctccaac 
      agcacgtca tcacgatac acacggacc gaaaagacc 
      acatttgcg agacctgcg gcaaacgtt tctcctcca 
      aaaccgcaa tgactgttc acgcaaaat cacacagtg 
      atgaacggt tgtacaatt gcaaattgt gcgattaca 
      agggccgga cggcctcag ccgtgtacg tccacatgt 
      ccacacacg cccgcgagg tgtgcgtgt gcgaggtct 
      gctcgaaaa tattcaaaa gcaaccgga acttgaacg 
      accatctca ggcgcgttc acagcaaag aaaagaagc 
      acaagtgca catattgcg acaagaagt tcgttgata 
      ggtacatgc tgacggtgc atgtgaggt gtcacacgg 
      gcgtgcgcc cctatgcct gccacttgt gcgagaaag 
      cgttcattc ggtcggacg ggctgaagg aacatttgg 
      caacgcatg gggagcgag tctggttta agtgcgata 
      atgggaaaa ggaagtcag ggaaagact acatttgcg 
      aagcgttga aagcaaatc tgggggaca ataattgca 
      caataatta ggatttcag ggccgttga aacgattgt 
      atgctgaaa gtcatgatt taacgactg gtgatgtaa 
      atgagatga tgactgatg gtccgctta aagaaaaat 
      ttcgcgcgg aaatgatcc aatggagcg atgaaatca 
      gagggagag attttgggt ttttttgca aagcggcca 
      cagatttgg ccgatttaa agccgtttt ggatcgtta 
      gcgacatta gacgcaaaa ctgatattc actggttca 
      aaaacacgt acaaagata agattatta aattaattc 
      gaattcaag cggatgaag tgactaggc aaaaaaggg 
      gatgggtct ttactgagg gagttgatg atgttacgt 
      ctgagtgtg acttagacg atttcagcg attgggact 
      taattatta aaaacaata atgcagaag agttgggag 
      agggattgg gcaaattaa tgcaatttc tcaattaag 
      tattatgac ttgtagtaa ttaggtgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY