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Gene information
General information
TC031003
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000404
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11898968..11900414
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031003
    iBeetle screen iB_09669

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031003 RA
      MYNPRLYTL CSSSHREKA HDPLREPLL GASSKMADS 
      ALNPAPDDD DETAETTTL QSVAPGVTP PTKTMDSRK 
      KPTSHQAAF STFFQHLTT TAHACILLY VILIIYFCF 
      STDFQFFTW HPLLLSVGW MFLMTEAIL SLSKDNLFT 
      GTLPKGVRV RLHWIFQSI ACVLITIGF VVVYVNKDY 
      LNKQHFKTW HGLLGLIGI VFCIPSCLN GVAALYHGS 
      LRQLVRPKI VKFVHILTG IVSFGFGGA ALILSVYTK 
      WFERHSSGN IVTFLLGLA FVSVAVMWT LVRPVITCC 
      RQLRHLM
    CDS sequence
    • >TC031003 RA
      atgtataat ccccggctc tacacactg tgctctagt 
      tcccatcgt gaaaaagcc cacgaccca ttacgagag 
      ccactcttg ggtgcaagc tcgaaaatg gcagattca 
      gccttgaat ccggcacct gatgacgac gacgaaacc 
      gccgaaacc actactctg cagtccgtt gcaccaggg 
      gtcactcca cccacgaaa acaatggac agtcgcaaa 
      aaacctaca tcacatcaa gccgcattc tccacattt 
      ttccagcat ttaacaaca acagcccat gcctgcatc 
      ctcctatac gtaattcta ataatttat ttttgtttt 
      tctacagat ttccagttt tttacgtgg catcctctc 
      ctcttatca gtgggatgg atgtttcta atgactgag 
      gcgattttg tcgctttcg aaagacaac ctgttcacg 
      gggacgcta ccgaaaggt gtcagagtg cgcctgcat 
      tggattttc caaagcata gcttgtgtc ttaataact 
      ataggcttt gtggtggtt tatgtcaat aaggattac 
      ttgaataag cagcacttt aagacatgg catgggctg 
      ctggggctc attgggatt gttttttgt attccgagt 
      tgtctcaac ggtgtagcc gcgctctac cacggcagt 
      ttgagacaa ctagttagg cctaagatc gttaaattc 
      gtgcatatt ttaaccggt attgttagt tttggtttc 
      gggggcgct gcactgatt ttgagtgtt tacaccaaa 
      tggtttgag agacattcc agtgggaac attgttact 
      tttctgtta ggcctagcc ttcgtttcc gtagccgtg 
      atgtggact cttgtacga cctgtgata acttgctgt 
      agacagttg aggcatcta atgtaa
    mRNA sequence
    • >TC031003 RA
      agcactagg agtttgcaa aaaatgaaa acgcaaaac 
      atttggaaa tataacaaa attgagttt tagattttt 
      gcgttttat ggtgaaaaa tggcggttt caaatatta 
      ccaaatctt acagccgct gcgatgtat aatccccgg 
      ctctacaca ctgtgctct agttcccat cgtgaaaaa 
      gcccacgac ccattacga gagccactc ttgggtgca 
      agctcgaaa atggcagat tcagccttg aatccggca 
      cctgatgac gacgacgaa accgccgaa accactact 
      ctgcagtcc gttgcacca ggggtcact ccacccacg 
      aaaacaatg gacagtcgc aaaaaacct acatcacat 
      caagccgca ttctccaca tttttccag catttaaca 
      acaacagcc catgcctgc atcctccta tacgtaatt 
      ctaataatt tatttttgt ttttctaca gatttccag 
      ttttttacg tggcatcct ctcctctta tcagtggga 
      tggatgttt ctaatgact gaggcgatt ttgtcgctt 
      tcgaaagac aacctgttc acggggacg ctaccgaaa 
      ggtgtcaga gtgcgcctg cattggatt ttccaaagc 
      atagcttgt gtcttaata actataggc tttgtggtg 
      gtttatgtc aataaggat tacttgaat aagcagcac 
      tttaagaca tggcatggg ctgctgggg ctcattggg 
      attgttttt tgtattccg agttgtctc aacggtgta 
      gccgcgctc taccacggc agtttgaga caactagtt 
      aggcctaag atcgttaaa ttcgtgcat attttaacc 
      ggtattgtt agttttggt ttcgggggc gctgcactg 
      attttgagt gtttacacc aaatggttt gagagacat 
      tccagtggg aacattgtt acttttctg ttaggccta 
      gccttcgtt tccgtagcc gtgatgtgg actcttgta 
      cgacctgtg ataacttgc tgtagacag ttgaggcat 
      ctaatgtaa ccgtttaca gtttcctat aattattaa 
      gcaaagaaa cgatatttt tctactgta tttacaaaa 
      ccgaaccaa tattgtgtg atacgttta tgtaactta 
      actattgta cttatacaa ttattttta ataaaaagt 
      attgacaca g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY