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Gene information
General information
TC000405 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11894592..11895919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000405
    iBeetle screen iB_09668

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000405 RA
      MRIIIVFFS LVLVNVSIA KKFKENEKP 
      EWAKKDIRD YTEADMERL LEQWEEDDE 
      PLEEDELPE HLRPLPKLD MSQLDPNNP 
      QSLLEASKK GRTLMTFVS VSGNPTREE 
      TEELTKLWQ TSLWNNHIQ AERYLVDDD 
      RAIFLFKDG SQAWTAKEY LVKQERCKG 
      VTIEGKEYP GEFANVKEE L
    CDS sequence
    • >TC000405 RA
      atgagaata ataatcgtt ttcttttcg ctggttttg 
      gtaaatgtt tcaatcgcg aaaaaattc aaagagaat 
      gagaaacca gagtgggct aagaaggat attagggac 
      tataccgaa gccgacatg gaacgttta ttggaacag 
      tgggaagaa gatgacgag ccattggaa gaagatgaa 
      ttgcctgaa catttacgt ccattgccg aaattagac 
      atgtcccag ctggacccc aataaccct caaagcctg 
      cttgaagcg tcaaaaaaa gggagaact ttaatgact 
      tttgttagc gtttctggg aatcccaca cgggaagaa 
      actgaagaa cttacaaaa ttgtggcaa actagtctt 
      tggaacaat cacattcaa gcagaaagg tatttagta 
      gacgacgac agggcgatt tttttgttt aaagatggg 
      tcacaagct tggactgcg aaggagtac cttgtgaaa 
      caagaacgg tgcaagggg gttactatc gaagggaaa 
      gagtatcca ggggaattc gcaaatgtt aaagaagaa 
      ttataa
    mRNA sequence
    • >TC000405 RA
      aaaattcca gtcaattta tcattacga tcttgattg 
      gaaaatttg gacaaacac ccccttaaa aatgtgttt 
      ttgaggtgt tatgatacg aaaccagat ttttgattc 
      ttatttgaa tggttattt tcttagtgt tggagaact 
      tgttaaatg tggcgccaa attacaaaa accggctat 
      tatcgtgtt gtcgaactc ggaaaagcc tccgatttt 
      gttgtaggt tatgattga tccaagttt ttatatcat 
      caagtaaaa atgagaata ataatcgtt ttcttttcg 
      ctggttttg gtaaatgtt tcaatcgcg aaaaaattc 
      aaagagaat gagaaacca gagtgggct aagaaggat 
      attagggac tataccgaa gccgacatg gaacgttta 
      ttggaacag tgggaagaa gatgacgag ccattggaa 
      gaagatgaa ttgcctgaa catttacgt ccattgccg 
      aaattagac atgtcccag ctggacccc aataaccct 
      caaagcctg cttgaagcg tcaaaaaaa gggagaact 
      ttaatgact tttgttagc gtttctggg aatcccaca 
      cgggaagaa actgaagaa cttacaaaa ttgtggcaa 
      actagtctt tggaacaat cacattcaa gcagaaagg 
      tatttagta gacgacgac agggcgatt tttttgttt 
      aaagatggg tcacaagct tggactgcg aaggagtac 
      cttgtgaaa caagaacgg tgcaagggg gttactatc 
      gaagggaaa gagtatcca ggggaattc gcaaatgtt 
      aaagaagaa ttataacgt aaaataaac ctttattaa 
      tatattttt gttacattt ttttaaata aatatttga 
      ggaaagaat gtgtttaat cagccttta aagtctcat 
      tttcagtag caacttgtt tctgtaact ctcgcacaa 
      ctgtggcta gtacttctg ggttaacac ctgcctcac 
      agagtctta cacaaatag ataaagctt caggatcta 
      gtccagtac ataacaaat ttgataatt ccattaacg 
      cctggaatg tttctttag cgtctttga ctcgtgttt 
      cgttgcttt cagacatct gaaacagca cagatgatg 
      atgctacaa agtataaaa tcggctaaa gtacctcca 
      aaattcaaa aactaactt atttttaag gttataata 
      atttaaacg cactatcac aaagcttca attttgtaa 
      gtgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY