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Gene information
General information
TC000461 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11030483..11034310
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000461
    iBeetle screen iB_09663

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000461 RA
      MESRDFDYP RSGVPQPQP GIRPLVDPE AHGEVDPSM 
      YPQPKEATH KIRGKSDVL EMIFGPVDQ ELLIVKSFY 
      FFFYSAFGS LFPLMGVYF KQMGMNAGQ CGFLIGIRP 
      FVEFLSAPF WGSLADRWQ KGKLLLLAS LAAWIIFTV 
      PLGSIQPPP TSCMEIKNN TPTLTAPNV NTRMINKRS 
      VMEQESEFE RYTRFERAT RPIPNAGQS PINVEFASN 
      YKPKDHESW VQPIISSIV YRTQDIQKA FFLLILLVI 
      IGEFFSAPA VTLADSAVI TLLGEDADR YGHQRMFGS 
      LGWGLAMFF VGIALDHST AFPNHPCGP DKKEKNYTI 
      CFATFSVLM GAALITATQ ITFKYDDPP PAPATTTDT 
      EKQPAPLSH EDQLQAQLA EQLQLPSLA DTRASVNAQ 
      PAEEKTKIF AQTTREMPE WVTVLQQFK NLKCASFLF 
      VAWFMGFGI GLIFTFLFW HLQDYGGSP TLFGVASVI 
      NHISEIFAY FFSFRLIRQ MGHVKVLCL GLIGNTLRF 
      LYISWLSNP WWVLPFEFM QGITHAAVW AACCSYLAH 
      NTPPQLRSS AQGVLQGIH HGLGRGCGA VIGGLFVNS 
      FGSTATFRG YGLICLVIL GAFVFINFY RVDQGFVSE 
      LPQTEDPRQ VAEETSHLA PHGVPSNPI PRALSSTRL 
      HELSQQDGY GATYQMGQG GTLDIPGGG GNNSFMQQS 
      SNDQRYSGF QMSSNLVQF PCGFSSEFT TLQTQQPLT 
      TASSSYDW
    CDS sequence
    • >TC000461 RA
      atggaaagt agggatttc gactacccc cgaagtggg 
      gttccccag ccccagccg gggatacgt ccgctggtg 
      gatccggag gcccacggc gaagtcgat ccttccatg 
      tacccccaa cccaaagaa gcaacgcac aaaatccgg 
      ggcaaatcg gacgttctc gagatgata ttcgggcca 
      gtggaccaa gagttgttg atcgtgaag agcttctac 
      tttttcttt tactcagct ttcgggtcg ctgttcccc 
      ctcatgggg gtgtacttc aaacagatg ggcatgaat 
      gcgggccag tgcgggttc ttgatcggg attcggccg 
      tttgtggag ttcttgtcg gcgccgttc tggggcagc 
      ttggccgat cggtggcag aaggggaag ttgctgctt 
      ctggcgtcg ctcgcggct tggatcatc ttcaccgtg 
      ccgttgggg tcgatacaa ccgcccccg acgtcgtgt 
      atggagatc aagaacaac acacctacc ctaacagcg 
      cctaatgtg aacaccaga atgataaat aagagatca 
      gtgatggag caagaatcg gaatttgaa cggtataca 
      aggtttgag cgcgctacg agaccgata ccaaacgcg 
      ggtcaatcg ccaattaac gtcgaattt gcgtccaat 
      tacaagccc aaggaccac gaaagctgg gtgcaacct 
      ataatctcc tccatagtt taccgcacc caagacatc 
      caaaaagcg ttcttcctc cttatcctc ctcgtgata 
      atcggcgaa tttttcagc gcccctgcc gtgacgttg 
      gccgactcg gctgtaatc accctccta ggggaagac 
      gccgatcgg tacggccac caaaggatg ttcgggtcc 
      ttgggctgg ggcctagca atgttcttc gttgggata 
      gctctagac cactcaacg gccttcccc aaccatccc 
      tgcggtccc gacaagaaa gaaaagaac tacacgatt 
      tgcttcgca actttttcc gtcctaatg ggggcggct 
      ttgataaca gcaactcag ataacgttc aaatacgat 
      gacccgcca ccagcgcct gccaccacc accgatacc 
      gaaaaacaa ccagcaccc ttgtcgcac gaagaccag 
      ctgcaagcc caacttgcc gaacagttg cagttaccg 
      tcacttgcc gatactcgg gcttcggtt aatgcacaa 
      ccagccgaa gaaaaaacg aaaattttc gctcaaacg 
      acccgcgaa atgcccgaa tgggtcaca gttctccaa 
      cagttcaaa aatctgaag tgtgcctcg tttcttttc 
      gtggcgtgg tttatgggc tttgggatc gggctgatc 
      tttactttc ctcttctgg cacttgcaa gactatgga 
      ggctcgccc actctcttc ggagtggct tctgttatc 
      aaccacata tcggaaatc tttgcctat tttttcagc 
      tttagactt ataaggcag atggggcat gtgaaagtg 
      ctctgtctt gggctgatt ggtaacacg ctacggttt 
      ttgtacatc tcttggctc tccaacccc tggtgggtg 
      ctaccgttc gaattcatg cagggaatc acccacgcc 
      gccgtctgg gcggcttgc tgctcgtac ctggcccac 
      aacacccct ccgcagctg cgttcgtcc gcccagggc 
      gtgctccag ggcatccac cacgggctt gggcgcggt 
      tgtggtgcc gtaatcggc ggcctcttc gtaaattcc 
      ttcggctcg acggccact ttccgcggc tacggtctc 
      atatgtctc gtgattctc ggagctttc gtctttatc 
      aacttctac cgtgtggat cagggcttc gtgtccgag 
      ttgcctcag accgaggat ccgagacaa gtggccgaa 
      gaaacgtcc catcttgcg ccccatggt gtcccaagt 
      aacccgatc ccgagagct ttgtccagc acccgactc 
      cacgagctt tcgcagcag gacggatac ggcgccacg 
      taccagatg ggccaagga gggactctg gatattccg 
      ggaggagga gggaataac tcttttatg cagcagagt 
      agcaacgat cagcggtat tcggggttc cagatgagt 
      tctaatttg gtgcagttt ccgtgtgga ttttcgtcc 
      gagtttacg actcttcag acgcaacaa ccactcacg 
      acggctagc tctagttac gattggtaa
    mRNA sequence
    • >TC000461 RA
      accgacgcg cgtctttta ttgatcgct cgcctacag 
      ctgctactg caactggcc atcgattcc accacacgg 
      aacacgcag tgttccagt gttccaagc tggtgttag 
      tgtccaaaa atggaaagt agggatttc gactacccc 
      cgaagtggg gttccccag ccccagccg gggatacgt 
      ccgctggtg gatccggag gcccacggc gaagtcgat 
      ccttccatg tacccccaa cccaaagaa gcaacgcac 
      aaaatccgg ggcaaatcg gacgttctc gagatgata 
      ttcgggcca gtggaccaa gagttgttg atcgtgaag 
      agcttctac tttttcttt tactcagct ttcgggtcg 
      ctgttcccc ctcatgggg gtgtacttc aaacagatg 
      ggcatgaat gcgggccag tgcgggttc ttgatcggg 
      attcggccg tttgtggag ttcttgtcg gcgccgttc 
      tggggcagc ttggccgat cggtggcag aaggggaag 
      ttgctgctt ctggcgtcg ctcgcggct tggatcatc 
      ttcaccgtg ccgttgggg tcgatacaa ccgcccccg 
      acgtcgtgt atggagatc aagaacaac acacctacc 
      ctaacagcg cctaatgtg aacaccaga atgataaat 
      aagagatca gtgatggag caagaatcg gaatttgaa 
      cggtataca aggtttgag cgcgctacg agaccgata 
      ccaaacgcg ggtcaatcg ccaattaac gtcgaattt 
      gcgtccaat tacaagccc aaggaccac gaaagctgg 
      gtgcaacct ataatctcc tccatagtt taccgcacc 
      caagacatc caaaaagcg ttcttcctc cttatcctc 
      ctcgtgata atcggcgaa tttttcagc gcccctgcc 
      gtgacgttg gccgactcg gctgtaatc accctccta 
      ggggaagac gccgatcgg tacggccac caaaggatg 
      ttcgggtcc ttgggctgg ggcctagca atgttcttc 
      gttgggata gctctagac cactcaacg gccttcccc 
      aaccatccc tgcggtccc gacaagaaa gaaaagaac 
      tacacgatt tgcttcgca actttttcc gtcctaatg 
      ggggcggct ttgataaca gcaactcag ataacgttc 
      aaatacgat gacccgcca ccagcgcct gccaccacc 
      accgatacc gaaaaacaa ccagcaccc ttgtcgcac 
      gaagaccag ctgcaagcc caacttgcc gaacagttg 
      cagttaccg tcacttgcc gatactcgg gcttcggtt 
      aatgcacaa ccagccgaa gaaaaaacg aaaattttc 
      gctcaaacg acccgcgaa atgcccgaa tgggtcaca 
      gttctccaa cagttcaaa aatctgaag tgtgcctcg 
      tttcttttc gtggcgtgg tttatgggc tttgggatc 
      gggctgatc tttactttc ctcttctgg cacttgcaa 
      gactatgga ggctcgccc actctcttc ggagtggct 
      tctgttatc aaccacata tcggaaatc tttgcctat 
      tttttcagc tttagactt ataaggcag atggggcat 
      gtgaaagtg ctctgtctt gggctgatt ggtaacacg 
      ctacggttt ttgtacatc tcttggctc tccaacccc 
      tggtgggtg ctaccgttc gaattcatg cagggaatc 
      acccacgcc gccgtctgg gcggcttgc tgctcgtac 
      ctggcccac aacacccct ccgcagctg cgttcgtcc 
      gcccagggc gtgctccag ggcatccac cacgggctt 
      gggcgcggt tgtggtgcc gtaatcggc ggcctcttc 
      gtaaattcc ttcggctcg acggccact ttccgcggc 
      tacggtctc atatgtctc gtgattctc ggagctttc 
      gtctttatc aacttctac cgtgtggat cagggcttc 
      gtgtccgag ttgcctcag accgaggat ccgagacaa 
      gtggccgaa gaaacgtcc catcttgcg ccccatggt 
      gtcccaagt aacccgatc ccgagagct ttgtccagc 
      acccgactc cacgagctt tcgcagcag gacggatac 
      ggcgccacg taccagatg ggccaagga gggactctg 
      gatattccg ggaggagga gggaataac tcttttatg 
      cagcagagt agcaacgat cagcggtat tcggggttc 
      cagatgagt tctaatttg gtgcagttt ccgtgtgga 
      ttttcgtcc gagtttacg actcttcag acgcaacaa 
      ccactcacg acggctagc tctagttac gattggtaa 
      taaaacact gcatcaccg aacatgtca taaatatta 
      ttcatttta ttcagcggt aagtaattg gtagtggaa 
      tgtaacgaa ggaaaacaa attatcgaa atctgatgt 
      tacaccatt gatctcata tcattttaa gtagaggtt 
      tatacaaag tgttgtagg actagcatt attagttgt 
      tgtaagcat tcgaatttg gagaggaaa atactctaa 
      tctatattt tatatgttt tttaaaacg tctataaaa 
      ctgaaatat cgttgtttc tatcaatgt tgcaaaagc 
      ggagtacat atgtggtat gcctctacg atttgcaaa 
      tatcttgcg ttaaccaag tgtgtaaaa acttatcga 
      tattgtaca aaatagttg ttaaagttg atttgtttg 
      tttttatat ttagacttt atttgttgt agatagtgt 
      acattatat ttaaagtgt ggcttcgtg gtcatgttg 
      tcgcactgg tacagtctt gcccttaga accagtttc 
      ataatttat taactacga ctgatacct aataacaac 
      aatcattgc aaataattg gaactggtt ttaaataat 
      ttctgttgc tcttaatca cagtatatt tgttgattg 
      taagtgtat aaactactc gaaaggtat agcttttta 
      ttgtttgcg cataaatgc aagggggaa ctgctagtt 
      gatgtttga tttaagcaa aaatgttcc gtcttgtgt 
      ttacgaaac actaagtga catccttag tccatacat 
      atccagaaa aagtattac ataacgaac agttgttga 
      aaacaatct attatttat ataaaaggc accttgtat 
      ttatctgta tatgtgaaa tataatata gtcgcacta 
      cgatgttac ttaatttta agctaaatt tatttatac 
      tcctaagtc tggataagt cgtcgcatt tgtttgaaa 
      aatgaaaaa taaaagacc tttaacaac tgaaaattt 
      tattacaaa cagtagtat tgctccgag gactgaaaa 
      attggtctt gatgaacac gtttattct cgttaactc 
      aatatacaa cagttttat atcacaaca aatggatag 
      aaaatacgt ctttagaaa tactgacag tgtagaaat 
      attcaagct acaagtcta ccgtgtctg tacaagaaa 
      aaactactg agacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY