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Gene information
General information
TC000480 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10784702..10801223
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000480
    iBeetle screen iB_09659

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000480 RA
      MATPSDSPI EEVVFEIEP SRVPKPVPV 
      ALEDLCRLT KFTRQEIRI MYRGFKTEC 
      PEGVVHEDS FKEIYSKFF PHGNSSLYA 
      HYVFKAFDV NCNGAISFR DLLITLSTL 
      LRGSVYERL RWTFKLYDV NGDGCISRG 
      ELSEIVVAV HELMGRRAH QVEDDRKAR 
      EQIDRVFRK LDLNQDGVI TIEEFMESC 
      LKDDVITRS LQMFDSVL
    CDS sequence
    • >TC000480 RA
      atggcaacg ccgtcagac agccctatc gaggaggtc 
      gtgttcgag atagagccg tccagggtg cccaagccc 
      gtgcctgtt gccctagag gacctctgc aggctcacc 
      aagttcaca cgacaggaa attagaatc atgtatcga 
      ggattcaaa acggaatgt ccagagggt gttgtccat 
      gaagatagc tttaaagaa atttattcg aaatttttt 
      ccacatgga aattcgagt ttgtatgca cactacgtg 
      ttcaaagcc ttcgatgtc aactgcaac ggagcaatt 
      agtttcagg gacctgctg ataacctta tcgacgttg 
      ctccgcggt tcggtgtac gagcgcctc cgatggacc 
      ttcaaactg tatgacgtg aacggtgac ggctgcatc 
      agccgcggc gaactgtcc gaaatcgtg gtggcggtg 
      cacgaactc atggggcgg cgcgcccac caggtggag 
      gacgaccgc aaggcccgc gagcaaatc gaccgagtc 
      ttccggaag ctggacctc aaccaggac ggcgtcatc 
      accatcgag gagttcatg gaatcgtgc ctcaaagac 
      gatgtgatc actaggtct ctgcagatg ttcgactca 
      gtgctgtga
    mRNA sequence
    • >TC000480 RA
      cgtaaccgt ttccatcgc cacacacaa cgtccacat 
      ccaacacca ataattaag tgatgcgtt agttagatt 
      aatatgtgg taataaaga cgcggagat cgtcgctcg 
      atagcttag gcctctatt aatagagag atggaccgg 
      aataaatgc gaatgcggc agatgatag aatagttgg 
      tgcttgcaa gagaatgct aacatgtgt cttccacaa 
      gcccacgac gaataaagg aagacatgg caacgccgt 
      cagacagcc ctatcgagg aggtcgtgt tcgagatag 
      agccgtcca gggtgccca agcccgtgc ctgttgccc 
      tagaggacc tctgcaggc tcaccaagt tcacacgac 
      aggaaatta gaatcatgt atcgaggat tcaaaacgg 
      aatgtccag agggtgttg tccatgaag atagcttta 
      aagaaattt attcgaaat tttttccac atggaaatt 
      cgagtttgt atgcacact acgtgttca aagccttcg 
      atgtcaact gcaacggag caattagtt tcagggacc 
      tgctgataa ccttatcga cgttgctcc gcggttcgg 
      tgtacgagc gcctccgat ggaccttca aactgtatg 
      acgtgaacg gtgacggct gcatcagcc gcggcgaac 
      tgtccgaaa tcgtggtgg cggtgcacg aactcatgg 
      ggcggcgcg cccaccagg tggaggacg accgcaagg 
      cccgcgagc aaatcgacc gagtcttcc ggaagctgg 
      acctcaacc aggacggcg tcatcacca tcgaggagt 
      tcatggaat cgtgcctca aagacgatg tgatcacta 
      ggtctctgc agatgttcg actcagtgc tgtgatgac 
      cggacggcg aaaagtgac ctggtggca ggagagatg 
      cgccagcct ggctgggac acgcgcggc ctttctgcg 
      gttgctcgg acgggaaca cggcgccgc agtcttagc 
      atcgtccat ccgtacact tcaacaact ttgcgttct 
      ttcgcccac tccagaccc aaaaagccc aaaaaatta 
      cacgaaaaa ctgaagcga ttccgtatt tggacaatc 
      agaagcttt cgcagaaga ttcagaaaa ttttgtttg 
      caattttcc aaaatgaac aggtttttg cgtctggag 
      gggcgaaaa gcacacttt acgagttcc atttttgta 
      acgacgagg agtcaaaac agtcttatg cttgaccat 
      cggctgatt agctcttag ttagctttg tatgttttt 
      taaggaaat ttactctta ctgtaatat tttaagggg 
      tgaacctgg agacaattc ctgcatata ttttgaaaa 
      ccttgctta tgatttgtc aattagagc taatattac 
      gaataaaac ttagataaa gaagatgtt gaattgaga 
      ttattatgt agcaattaa tccaggcca aacctaaag 
      tgactgtca gttgtgaat gtgactgta attaggtac 
      gtaacgagc ctatgattg aaataattt tgatctgaa 
      gttgtttcg atcgtataa taccccaat caacagacc 
      gggtctgtc cgcctaatt taaagtgct aagagaata 
      aaatcgtgg gtctacatg cacaggtgt ctgattctc 
      ttcctgaat ttatttaat tctgaagtg cctctttgc 
      cttttcttt atgaattaa taaactgta agacgtaaa 
      tgtaaattt taacataat ttactccca aacgacaaa 
      tatttttga taaaagaac gtcacatta ttattggct 
      tttgagaaa tagtacaaa aaacttgaa attattttt 
      gtgtcagcg tacacgaac cacaaaatt gtaacgaaa 
      ttttacacc ctttattac acacgcggt ctcgtaaat 
      ttattgtaa aagtgaata ttcgaaaat ctggaagta 
      cttcagaat gatattcga tttttcccg tagttcagt 
      aacactgca caagttcaa agcacgtgt gtgtttgaa 
      cttgtgagt aaggtacaa gaattgtaa atttatccg 
      tatttacat cagtttagc ttaagatcc ttgttcttc 
      cttaagtgg ttaaagtga atagacttc atcgaatta 
      ctgtttaaa atagaaact gttggacta aaatttcgt 
      tttgatcgt tgtagatat taggagata agttcggct 
      tttatttag tttagttaa ataaatatc taggaaatg 
      ttaatcaaa atatacgat tattatatg atatagtga 
      aatatatcg taacagtag atgtcttaa aatcgattt 
      ctttctgtt atctgttat tgcgttcag tcagcaaaa 
      gagcaatgt ccgtaaatt accaaatat gcaatacat 
      cacatgcat atcgagaac tacaatcac aagagccaa 
      ccatcacta ctgatttgc caacaaaat gagcttttt 
      aaatgatac ggttaaatg tggtaatag acttaagat 
      ttcttaaac tgtattaat tttttacta ttctttgta 
      taacaagat ggccagacg gcactaaca cacttcata 
      aacatgtaa ctgtgtaaa attttaatt aaaaagtga 
      cgataggat taacaaatt tcatacgtg ctgtactat 
      aaatgtatt aattaatta aaatatatt ttaaattca 
      gggcacaat taattacat tactactat tttcgcatc 
      agaggcatg tcccataaa acaacttat gcgaaaaat 
      tttacatga agtcggttt aaaaagttg caattaata 
      tgtttctcc ccagaatca gcaatattc gcgcagtac 
      acataattt tgactgact aaaaaccaa attatatta 
      ttgttataa agtttaatt aataaattg ttatacggt 
      gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY