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Gene information
General information
TC000521 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10219650..10227531
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000521
    iBeetle screen iB_09650

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000521 RA
      MKIAFLLAY AILTSKRVR SAAVQAPLD TISSEEITD 
      ADRKTLEFL NQYFPLRPK REETTDSDP KPLELLNQY 
      FPPSTKKAD ITDSDPKPL ELLNQYFPP STKKADITD 
      SDPKTLEFL NQYFPQSVK RSDITESDK KAIEFLNHY 
      LAPRPKRSG EEALHHLKA SISHGIKSK LGLIAKSSA 
      SASAHFSKS SSSGHDHHH HHYHHHDEH PEFYDHHHK 
      HFDFWSLKK SILNTLLQA VKAIKGGVI AIKGQLIKG 
      SGYLISAKG KLISAKGEA ITTLGKNIA TSAILSPTH 
      GHSDDYGHG HYHHEEYGS PSAPGPEYE VPEHHYHHH 
      SPHHEDKPD ADHAGILIL KKIPKHEHE HEHDHLPVE 
      HKIPDFKPI GHSGPGLGE IIGKFFSAS TSLKPGEPA 
      PFGDHYDHD HHHEHYHSD HDHDHDHHH DHDEHYDHS 
      SHSSHSEYH QETHPSSHD DDHDFKHSD ADHHNIHDN 
      PSSHYGSPF EEAPAYLNI EEYASESKK QAQTVTQAP 
      TAEPQKIQN AQQPQTPIK YDEIRPLPT AFANTDPQS 
      IKYDEIRPL SAFSNTGID TSYLQNNDN RPNLFVGSY 
      SNKPNTAFP PYAQQLQSV RPTALALDT ASFGGFPNT 
      GVSPTSGSD IKYLAPPGV GPQVFPSGV NTQRYPQSV 
      KYQGLLDSF PSPSHKTPL KRPTRSKHH HGRKRPTDE 
      LTVLAGYYN YYEHFDPYF
    CDS sequence
    • >TC000521 RA
      atgaagata gcatttctc ctggcctat gcaattctt 
      acatcgaaa agggtcaga agtgccgca gttcaagcc 
      cccttagac acaatttcc tcggaagaa atcacagac 
      gcggaccga aaaacttta gaattttta aaccaatat 
      tttcctctc aggcctaag cgagaagag accacagac 
      tcggaccca aaaccttta gaattgtta aaccagtat 
      tttcctccc agcaccaag aaagcagat atcacagac 
      tctgacccg aaaccttta gaactgtta aaccagtat 
      tttcctcca agtaccaag aaagcagat atcacagac 
      tcagaccca aaaacttta gaattccta aatcagtat 
      tttcctcaa agtgttaag agatcagat atcacggag 
      tcggacaaa aaagctata gaattctta aatcattat 
      ttggcccca aggcccaaa aggtcaggt gaagaggcc 
      ttgcaccat ttaaaagcc tctataagt catggcatt 
      aaatcgaag ttgggacta atagccaag agttcagct 
      tcagcaagc gcccatttt agtaaaagt agcagcagc 
      ggccatgac catcaccat catcattat caccatcac 
      gatgaacat ccggaattc tatgaccat catcacaag 
      cacttcgac ttctggtct ttgaagaaa tccatcttg 
      aacactctc ctccaagcg gttaaagcc ataaaaggc 
      ggtgtgata gccataaaa gggcaactg ataaagggc 
      agcggttac ctcatttca gcgaagggc aagctaata 
      agtgccaaa ggggaagct ataacaact ttaggcaag 
      aacattgcc acctcagcc attctaagc ccaacccat 
      ggtcattca gacgactac ggtcacggc cactaccac 
      cacgaagaa tatggatca ccgtcggct ccggggcct 
      gaatacgag gtgccagaa caccactat catcatcat 
      tcgccacat catgaagac aaaccggac gccgaccac 
      gctggtatc ttaatcctg aagaagatc cccaagcac 
      gaacacgaa cacgaacac gatcatcta cccgtcgaa 
      cacaaaatt cccgacttc aaaccgatc ggccactca 
      ggacccggt cttggagaa atcatcgga aaattcttc 
      tccgcatcc acctcccta aaaccagga gaacccgct 
      cctttcggt gatcactac gatcatgat catcaccac 
      gaacattat cattccgat cacgatcac gatcacgat 
      caccaccat gaccacgac gagcattac gatcactct 
      agtcactca tcccactca gagtaccac caggaaact 
      cacccctct agtcacgac gatgatcac gattttaag 
      cactctgac gctgatcat cacaatatt catgataac 
      ccttccagt cattacggg agccccttc gaagaagct 
      cccgcttac ttaaacatt gaggagtat gcttccgag 
      tctaagaaa caagcacag actgtcact caagcccca 
      actgccgaa ccgcaaaaa atacaaaac gcccaacaa 
      ccacaaact cctattaaa tatgacgag attagaccg 
      ctaccaact gcctttgcc aatactgac ccccagagt 
      atcaaatac gacgaaatc aggccttta tccgctttt 
      tccaacaca gggattgac acttcttac ctccaaaac 
      aacgacaat cgcccaaat ttattcgtt ggctcttat 
      tcaaataaa ccgaatacg gcttttcca ccatacgcc 
      caacagttg cagtcagtc agaccaact gctctagct 
      cttgacact gcctcattt ggtggtttt cctaacacg 
      ggtgtttcc ccaaccagt ggttccgat atcaaatat 
      ttagcacct ccgggtgtt ggaccacaa gtgtttccc 
      agtggtgtg aacacacaa agataccca caaagtgtt 
      aagtaccaa ggtttgctc gatagtttc ccttcgccg 
      tctcataaa accccttta aaacggccc acacgtagc 
      aagcaccac catggccgt aaaagacct actgacgaa 
      ctgactgta cttgcaggt tactacaac tactacgaa 
      cactttgac ccgtatttc tga
    mRNA sequence
    • >TC000521 RA
      cttgaagtg agagtgaag agtttgcag tgaacggct 
      cacggtgtc ttcgacgag cacgagtgt atatgtgtg 
      tgtgacctg gacctagac tttgccatt atgaagata 
      gcatttctc ctggcctat gcaattctt acatcgaaa 
      agggtcaga agtgccgca gttcaagcc cccttagac 
      acaatttcc tcggaagaa atcacagac gcggaccga 
      aaaacttta gaattttta aaccaatat tttcctctc 
      aggcctaag cgagaagag accacagac tcggaccca 
      aaaccttta gaattgtta aaccagtat tttcctccc 
      agcaccaag aaagcagat atcacagac tctgacccg 
      aaaccttta gaactgtta aaccagtat tttcctcca 
      agtaccaag aaagcagat atcacagac tcagaccca 
      aaaacttta gaattccta aatcagtat tttcctcaa 
      agtgttaag agatcagat atcacggag tcggacaaa 
      aaagctata gaattctta aatcattat ttggcccca 
      aggcccaaa aggtcaggt gaagaggcc ttgcaccat 
      ttaaaagcc tctataagt catggcatt aaatcgaag 
      ttgggacta atagccaag agttcagct tcagcaagc 
      gcccatttt agtaaaagt agcagcagc ggccatgac 
      catcaccat catcattat caccatcac gatgaacat 
      ccggaattc tatgaccat catcacaag cacttcgac 
      ttctggtct ttgaagaaa tccatcttg aacactctc 
      ctccaagcg gttaaagcc ataaaaggc ggtgtgata 
      gccataaaa gggcaactg ataaagggc agcggttac 
      ctcatttca gcgaagggc aagctaata agtgccaaa 
      ggggaagct ataacaact ttaggcaag aacattgcc 
      acctcagcc attctaagc ccaacccat ggtcattca 
      gacgactac ggtcacggc cactaccac cacgaagaa 
      tatggatca ccgtcggct ccggggcct gaatacgag 
      gtgccagaa caccactat catcatcat tcgccacat 
      catgaagac aaaccggac gccgaccac gctggtatc 
      ttaatcctg aagaagatc cccaagcac gaacacgaa 
      cacgaacac gatcatcta cccgtcgaa cacaaaatt 
      cccgacttc aaaccgatc ggccactca ggacccggt 
      cttggagaa atcatcgga aaattcttc tccgcatcc 
      acctcccta aaaccagga gaacccgct cctttcggt 
      gatcactac gatcatgat catcaccac gaacattat 
      cattccgat cacgatcac gatcacgat caccaccat 
      gaccacgac gagcattac gatcactct agtcactca 
      tcccactca gagtaccac caggaaact cacccctct 
      agtcacgac gatgatcac gattttaag cactctgac 
      gctgatcat cacaatatt catgataac ccttccagt 
      cattacggg agccccttc gaagaagct cccgcttac 
      ttaaacatt gaggagtat gcttccgag tctaagaaa 
      caagcacag actgtcact caagcccca actgccgaa 
      ccgcaaaaa atacaaaac gcccaacaa ccacaaact 
      cctattaaa tatgacgag attagaccg ctaccaact 
      gcctttgcc aatactgac ccccagagt atcaaatac 
      gacgaaatc aggccttta tccgctttt tccaacaca 
      gggattgac acttcttac ctccaaaac aacgacaat 
      cgcccaaat ttattcgtt ggctcttat tcaaataaa 
      ccgaatacg gcttttcca ccatacgcc caacagttg 
      cagtcagtc agaccaact gctctagct cttgacact 
      gcctcattt ggtggtttt cctaacacg ggtgtttcc 
      ccaaccagt ggttccgat atcaaatat ttagcacct 
      ccgggtgtt ggaccacaa gtgtttccc agtggtgtg 
      aacacacaa agataccca caaagtgtt aagtaccaa 
      ggtttgctc gatagtttc ccttcgccg tctcataaa 
      accccttta aaacggccc acacgtagc aagcaccac 
      catggccgt aaaagacct actgacgaa ctgactgta 
      cttgcaggt tactacaac tactacgaa cactttgac 
      ccgtatttc tgacgaagg cgaaccagg aactttttg 
      tacataatt ttaacactc taatgaatt taggtacct 
      ctgtagggt ttcccatct acgtaattc tattctctt 
      atttattgt tgtacctaa tgtaaaaat gtaagttta 
      tattttgta aaatgttaa tattaataa ataaattga 
      aaccga
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