Status
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Gene information
General information
TC032141
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001025,TC001024
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9941268..9949480
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032141
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09645

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_07304

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 3

    yolk deposition irregular - Number of animals showing the phenotype: 3

    vitellarium stage sequence irregular - Number of animals showing the phenotype: 1

    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032141 RA
      MNPKKPREQ TPKSRFKNY PQEKNLPSN IWTCLFLIS 
      LTTNYFITT FAYVVLKDP IDGKIFWLI VLLDAVFAL 
      DFFLNVLNF IWTKHKSPH KQPLRSEMI LTIDLVLAF 
      PYSYIYENF AHNYNKTVF LYLRLVSVF RLFHLVLFF 
      KEKWNTAGI NHLKCFTFQ FLIYFTMRM HAATCLLHY 
      STLGTDLAG KYNTASEQY VICFYFALM DITNRNFGD 
      LTPWGVTEK ILSILIMAV GFTVTAIFI ASLALLLDN 
      KYKRRTSLK SHFRTVLNF LKSIPIDPK QIQDYYKIL 
      WEKKNGAHQ ASKYATLPY PLKCEIGVD VNIRFFQTS 
      LLFQDKPDA FLRRVCNLM KHEFHQAHQ LIYQRNVVK 
      NKTIFVVLG TLEILSDED DESPIVTLT SGTCLGESS 
      LILSLPAKC SVRTSSYTE LQVLYRRDF ATILDVHPI 
      IYNNIREAI LQRIVDSQE VKMVEDEQI NLHPFFPKS 
      DKVSVKDLK DKLRPKVRF GENRYVTLY RLEDSAKKN 
      QKFPWILQP ESPVRQSWE YLILLVAFY VNITYPYYI 
      IFVKSYPIW FSFFSNFID LILVLDLVV ILTTAIEER 
      NEKIDTFMS ITQKRVIQP GFYLDFFSC VKTELFYWI 
      WTRNERFGD VLKLNRLVK IYRIPKLIR KYENKIEAN 
      FHGLTLLKH LLYLLVLTY WFGGCLYLV TCFDNSCTE 
      DGWFFTKLL KDFKEQQTS YITDDSLLT SLYFSLSCL 
      TGFTPGDIT PVNLTDRVI TCFGFISGL FMFSCCVAQ 
      FAAALARKI KMRLDFQER FCPVLENLK ERNLSTHLV 
      KRVKNYFLV QWMYDKASI FRQHLFYNL DPNIQKVVQ 
      VEEKMDTIL SIPLFRKLD KNFLVALVT KTPVLVLPP 
      EEFITYKGE FLKVLYIVM RGHCIRQFE KTSVLLGPK 
      SFFGTLAFF SDVNSLCTV KTVTHVKLL YITEEIFWA 
      AASMFPRVA KRFGASSEN FDLITEFRK IPIRGSLDN 
      NLELDNELW LSKDSLLSV ISYCKERKQ SYSAPFRQL 
      GVLCWVQMF LLPICILPY GRLIKVWVT GRLLVTLTV 
      AILIPYQLI LSPYTSTID TVIFVADLI AYLDLYFNL 
      FVGYYGPKN QLIIHPLKT ATHYLKGKF LLDFVLCFP 
      WEMLVKHFS GNYYKVAKI IRLGQIYRL FQFFNYVST 
      FTKKNKYGV LLHILKFIP LVIIFVHFW ATIFVINTC 
      NYAPNGTFL CQENSWIDR SKFQNFAHP LEVYSTSIY 
      FCTNVLTFT GLGDITPKY PSSVIITIV MQICGILFI 
      GYVYAKIAS YKTHWNSIL FKTHETMNE LKKFMVQEN 
      VTKNLQMQI VQQLEYRWK RNKLEPNTA LAKFHPNLY 
      EDCVYAMFD SIFESVPVF SNIDKSFLR LLGTIVKEQ 
      CFRHHEVIF RADDIVSEL FILERGTIK IVEKLGGME 
      LHLEAGAIF GNLTPRALH KVSKTVTAL SNVDVLCIN 
      TEEFYLALD KFPWIQNAI EANLTNEEF MLHEIRNDL 
      SETSLTKSN HDSATSFNI LKTSSEIKL NFVGCFLLC 
      LPWKFFSIT IPEYSLFIK IFDVYCLFL AHVNSLLVL 
      YEFSFQNHN NFLLGVAIY TDVTFILKI LIEMHKTYE 
      NRFGEHITS NKKIVSRYF RNKKLKRAV DIFVNFPFY 
      YLTFFAAVF TSFDANFYF SCLRLITLY RLIYFWEYY 
      KSKASQLLV KISLLKIGA LIVWGTLVI HIFSCGWYL 
      CACPLMTCF KDSWVNEVF NTTSPHFSK KLDLYVTSL 
      YFVITVMTT TGTGDISAN SIREQVFIT LMMLTAVFL 
      TGIFIGEIW NCLNSTTIA RLKYDNEIN ELKQYFKNS 
      NVSDYQMKK MWRYVQQIW TVSRGNQVP RALSLLHSS 
      ALQELMLQI YGPHISNSY VFSEVEKSF TRQLCQYLK 
      RRVYFPGNY IVQHGDFDG TMYFIHYGE VEILTVHMN 
      LTETIHEVL RRGEMFGVM QGLFYDIPH HFFFRARTA 
      VHLVALQFD DWRHLLEFF PESRDAIFA KTNRVQVST 
      VKKITKHLV FYEKFKTYS TMSTVGPFR LLTYTRQQV 
      LRFQLNYPR CNYAKKETG SVSMIGGAL KKKKLGKMG 
      PVMEKKVIP VETDPQKLV KFVCGSNIY TTGQDIELK 
      PDNEYPEWL WNIRTGPPP PLEELDPNT KEYWRRIKK 
      MAMRRNNKL AQLKKF
    CDS sequence
    • >TC032141 RA
      atgaatccg aaaaaacct agagaacaa accccaaaa 
      agccggttc aaaaattac ccccaagaa aaaaacctc 
      ccgtctaac atatggacc tgtttattc ctgatctcc 
      ctcaccacg aactacttt attacaacc tttgcttac 
      gtggtcctg aaggaccca atcgacggg aaaatcttc 
      tggctgatc gtcctcctg gacgcagta tttgcttta 
      gattttttc ctcaacgtt ctgaatttt atttggacc 
      aaacataag tcgccacac aagcagccg ttaagaagc 
      gagatgatt ctaacgata gacttggtc cttgcgttt 
      ccttattcg tacatttac gagaatttc gctcataat 
      tataataag acagttttc ctgtatttg cgactagtt 
      tcagtgttt cgtttgttt catttagtg ctgtttttt 
      aaagagaag tggaacaca gccgggatt aatcacttg 
      aaatgtttt acttttcaa tttttgatt tattttacg 
      atgcggatg catgcggcc acgtgcctt ttacattat 
      tcgactttg ggaacggat ctcgccgga aaatacaac 
      acggcttcg gagcaatac gttatttgc ttttatttc 
      gctttgatg gatattacg aatcggaat ttcggcgat 
      ttaactccc tggggagtg actgaaaag attctttca 
      attttaata atggcagtg gggttcaca gtgactgca 
      atttttatc gcaagtttg gcccttctg ctggacaat 
      aagtacaaa cgaagaacc agtctcaag agtcacttc 
      cggactgtt ttaaacttc ctcaaatca ataccaatc 
      gatccgaaa caaatccaa gactattac aaaattcta 
      tgggagaag aaaaacggg gcgcaccaa gcctcaaaa 
      tacgccact ctcccgtac cccctaaaa tgcgaaatt 
      ggggtcgat gtaaacatc cgttttttc caaacttct 
      ctgctcttc caagacaaa cccgacgcg tttttgcgc 
      cgagtttgc aatttaatg aaacatgag ttccaccaa 
      gcgcatcaa ctaatttat cagcgcaat gttgtcaaa 
      aataaaaca attttcgta gttctgggc actctcgaa 
      atcttatca gacgaagac gacgaaagc cccatcgtc 
      accctaact tcagggacg tgtctgggc gaaagctcc 
      ctaattttg agcctccca gctaagtgt tcagttcgc 
      acgtctagc tacacagaa ttacaagtt ctgtaccga 
      agggatttc gctacaatt ctggacgtg caccccatc 
      atttacaac aatatacgc gaggctatt ttgcagaga 
      attgtcgac tcccaagag gtcaaaatg gttgaggac 
      gaacaaatc aacttgcat ccgttcttc cccaaaagt 
      gacaaagtt tcggtgaag gatttgaaa gacaagttg 
      cgaccaaaa gtcagattt ggggaaaat cgttacgtg 
      actttgtat cgcttggag gattcggcc aaaaaaaat 
      caaaaattt ccgtggatc ctccaaccg gagtctcca 
      gtcaggcag tcttgggag tatttgatt ttgctcgtg 
      gctttttac gttaacatc acttatcct tactacatt 
      atcttcgtc aagtcttac ccgatttgg ttcagtttt 
      ttctcaaat ttcattgac ttaatcctt gtcttggac 
      ctcgtggtt atcctgacc acggcaatt gaggaaagg 
      aacgaaaaa atcgacact ttcatgtcc attacgcaa 
      aaacgggtg attcaaccc gggttctac ttggacttt 
      ttcagttgc gtcaaaacc gagttgttt tactggatt 
      tggacacga aacgagcga tttggcgac gttttaaaa 
      ctgaaccgt ttggtcaaa atttatcga atcccgaaa 
      cttatccgg aagtacgaa aacaagatc gaggcaaat 
      tttcacggt ttgactctg ctgaagcat cttttgtac 
      cttctggtg ctaacttat tggttcggt ggttgcctg 
      taccttgtg acctgtttc gataattcg tgtactgaa 
      gacggttgg tttttcacg aaactgttg aaagacttc 
      aaggagcaa caaacgagt tacattact gatgattcc 
      ttgttaact tctttgtac ttttcgctg tcatgcttg 
      accggtttt acgcctggt gacatcaca ccggttaac 
      ttaaccgat cgggttata acctgtttt gggtttatt 
      tcgggcctc tttatgttt agctgttgt gtggcgcaa 
      ttcgccgct gctttggca aggaaaata aaaatgcgg 
      ttggacttc caagagagg ttttgccca gttttggaa 
      aatctcaaa gagagaaac ttgagtact catttggtt 
      aagagagtc aaaaactat tttttggtc cagtggatg 
      tatgacaaa gcctcgatt ttccgccaa cacttattt 
      tacaatctc gacccaaac atccaaaaa gtcgtacaa 
      gttgaggag aaaatggac acgattttg tcaatccct 
      ttatttcga aaattggat aaaaatttc ttggtggct 
      cttgtgact aaaacaccg gttctggtc ctaccaccc 
      gaagagttc ataacgtac aagggcgaa tttttaaaa 
      gtgctctac atagtaatg agaggacac tgcattcgg 
      cagtttgaa aaaacctcg gtcttgttg ggccctaag 
      tcattcttc ggcactttg gcgttcttc tcagacgtt 
      aattccctg tgtactgtt aaaacagta actcacgtt 
      aagcttctc tacatcact gaagaaatt ttctgggcc 
      gcagcttca atgtttcca agagttgca aaacggttc 
      ggtgcaagt tcggaaaat tttgacttg atcactgaa 
      tttcgaaaa ataccgata cgtggctcg cttgataat 
      aatttggaa ctcgataac gagctctgg ttgtccaaa 
      gattcactt ttgagcgtt atttcatat tgtaaggag 
      cgtaaacag agttatagc gcgcctttt cgacaactg 
      ggagtcttg tgttgggtc caaatgttt ttgttaccc 
      atctgtatt cttccatat gggcgatta atcaaagtt 
      tgggtcacc ggacgactg ttagtgacc ctaactgtg 
      gcaattttg ataccgtac caattgatt ctaagcccc 
      tacacttct accattgat acagtcatt ttcgtggcc 
      gatttaatt gcatatttg gacctctat ttcaattta 
      tttgtcggt tattacggt cctaaaaat caactcata 
      atacatccc ttgaaaaca gcgacgcat tacttgaaa 
      ggaaagttt ttgctagac tttgtgctt tgttttccg 
      tgggaaatg ctagtgaaa cacttttct ggaaattat 
      tacaaagtc gccaaaatc attcgactt gggcagatc 
      taccgacta ttccaattt tttaactat gtgtccaca 
      tttaccaaa aagaataaa tacggagtt cttctccat 
      attttgaaa tttattcca ctggtgatt atttttgtt 
      catttctgg gccacaatt ttcgtcata aacacttgt 
      aattacgcc ccaaatggg acattttta tgccaagaa 
      aactcatgg attgatagg tccaaattt caaaatttc 
      gctcaccct cttgaagtg tattccact tcgatttat 
      ttctgcacc aatgttttg acttttacc ggtttaggg 
      gacattaca ccgaaatac cccagctct gtgattatc 
      acaatcgtg atgcagatt tgcggcatt ttgttcatc 
      ggttatgtg tatgcaaaa atcgcgagc tataagacg 
      cactggaac agcattctc ttcaaaacc cacgaaacg 
      atgaacgag ttgaagaaa tttatggtc caggaaaac 
      gttacgaaa aatctccaa atgcaaatc gtccagcaa 
      ttggaatat cgctggaag cgcaacaaa ctcgagcca 
      aacacggct ttggccaaa ttccaccca aatctttac 
      gaggattgc gtttacgca atgtttgac agtattttc 
      gaaagcgtc ccggttttt tcaaacatc gataagtcg 
      tttctgaga cttttgggc acgattgtg aaagagcag 
      tgttttcgt caccacgag gtgattttt cgagccgat 
      gatatcgtg tcagagttg tttatactt gagagaggg 
      actatcaaa attgttgaa aaacttgga ggaatggaa 
      ttgcacttg gaagccggc gcgattttt ggcaatttg 
      acaccaagg gctttgcat aaagtttca aaaactgtg 
      acagctttg agtaatgtt gatgtcctg tgtattaac 
      acagaagag ttctatttg gcactagat aagtttcct 
      tggattcaa aatgcgatt gaggcgaat ctcacaaac 
      gaagagttt atgttacac gagattaga aacgacctc 
      tctgagaca agtcttaca aaatcaaac cacgattct 
      gctacaagt ttcaacatt ttaaaaacc tcatcggaa 
      atcaaatta aatttcgtc gggtgtttt ctgttgtgc 
      ctcccttgg aaatttttc agtattaca attccagaa 
      tactccctc tttattaaa atattcgat gtctactgc 
      ctgttccta gctcacgtc aattcactt ctcgttttg 
      tacgaattc tcgttccaa aatcataat aattttttg 
      ttaggtgta gccatctac actgatgtt acttttatt 
      ttgaaaata ttaatcgaa atgcacaaa acttacgag 
      aacaggttt ggagaacac attacttcg aacaagaaa 
      attgtctcg cgatacttc cgtaacaag aagctgaaa 
      cgcgctgtt gacattttt gtgaatttt ccgttttat 
      tatttaact tttttcgct gctgtattt actagtttt 
      gacgcaaat ttttatttt tcatgtctc agacttatc 
      accctttac agattgatt tatttttgg gagtactac 
      aagagtaaa gccagtcag ttgttagtc aaaatttcg 
      cttctcaaa atcggtgct ttgattgtt tgggggacg 
      ttggtgatt catattttt tcatgcggg tggtatcta 
      tgtgcgtgt cctttaatg acttgcttt aaagattct 
      tgggttaat gaggtcttt aacacaacc tcgccacac 
      ttttcgaaa aaactagat ctttatgtt acttcgtta 
      tatttcgtg attactgta atgacaacg acgggaact 
      ggcgatatt tccgccaac agcatccgt gagcaagtt 
      ttcatcacg cttatgatg ctaactgcg gtttttctc 
      actggaatt ttcatcggt gagatttgg aattgtctc 
      aattccact acaatcgca cgattaaaa tacgacaac 
      gaaatcaac gagcttaaa cagtatttt aaaaacagc 
      aacgtgagt gattaccaa atgaaaaaa atgtggcgc 
      tacgtccaa caaatctgg acagtcagt cgaggaaat 
      caagtacca cgcgcactc tccctttta cattcttct 
      gctttacaa gaacttatg ttacaaatt tacggacct 
      catatttca aactcgtac gtgttttcg gaggtcgaa 
      aagtcattc acccgccag ttatgccaa tatctcaag 
      cgtcgtgtg tatttcccg gggaattac atagtccaa 
      catggcgat ttcgacggc accatgtat ttcatccat 
      tatggagaa gtcgaaata ctgaccgta catatgaat 
      ttaacagaa actatacat gaggtgtta cgtagagga 
      gaaatgttt ggagtgatg cagggttta ttttacgat 
      attccgcac catttcttt tttcgcgcc cgcactgct 
      gtccattta gtggctttg cagtttgat gactggcga 
      cacttactg gagtttttc cccgaaagt cgggacgcc 
      attttcgca aaaacgaac agagttcaa gtgtcaact 
      gtcaaaaaa ataaccaaa catttggtt ttttacgaa 
      aaattcaaa acctattct acaatgagt actgtgggc 
      cccttcaga ctattaaca tatactcgt cagcaagtt 
      ttacggttt cagttaaac tatcctcga tgtaattac 
      gcgaaaaaa gaaaccgga agcgtcagt atgattgga 
      ggggctctg aagaagaaa aagttgggg aagatgggt 
      ccagtgatg gaaaagaag gtcattcca gttgaaaca 
      gaccctcag aaacttgta aaatttgtt tgtggctcg 
      aatatttac actactggt caagacatt gaattaaag 
      cccgataat gagtatcct gaatggttg tggaatata 
      cgaacaggg cccccgccg ccactggaa gaactagac 
      ccaaatact aaggaatat tggagaagg ataaagaaa 
      atggcaatg agacgaaat aataaatta gcacaactt 
      aagaagttt taa
    mRNA sequence
    • >TC032141 RA
      aaacgtcac agatcgcaa tgaatccga aaaaaccta 
      gagaacaaa ccccaaaaa gccggttca aaaattacc 
      cccaagaaa aaaacctcc cgtctaaca tatggacct 
      gtttattcc tgatctccc tcaccacga actacttta 
      ttacaacct ttgcttacg tggtcctga aggacccaa 
      tcgacggga aaatcttct ggctgatcg tcctcctgg 
      acgcagtat ttgctttag attttttcc tcaacgttc 
      tgaatttta tttggacca aacataagt cgccacaca 
      agcagccgt taagaagcg agatgattc taacgatag 
      acttggtcc ttgcgtttc cttattcgt acatttacg 
      agaatttcg ctcataatt ataataaga cagttttcc 
      tgtatttgc gactagttt cagtgtttc gtttgtttc 
      atttagtgc tgtttttta aagagaagt ggaacacag 
      ccgggatta atcacttga aatgtttta cttttcaat 
      ttttgattt attttacga tgcggatgc atgcggcca 
      cgtgccttt tacattatt cgactttgg gaacggatc 
      tcgccggaa aatacaaca cggcttcgg agcaatacg 
      ttatttgct tttatttcg ctttgatgg atattacga 
      atcggaatt tcggcgatt taactccct ggggagtga 
      ctgaaaaga ttctttcaa ttttaataa tggcagtgg 
      ggttcacag tgactgcaa tttttatcg caagtttgg 
      cccttctgc tggacaata agtacaaac gaagaacca 
      gtctcaaga gtcacttcc ggactgttt taaacttcc 
      tcaaatcaa taccaatcg atccgaaac aaatccaag 
      actattaca aaattctat gggagaaga aaaacgggg 
      cgcaccaag cctcaaaat acgccactc tcccgtacc 
      ccctaaaat gcgaaattg gggtcgatg taaacatcc 
      gttttttcc aaacttctc tgctcttcc aagacaaac 
      ccgacgcgt ttttgcgcc gagtttgca atttaatga 
      aacatgagt tccaccaag cgcatcaac taatttatc 
      agcgcaatg ttgtcaaaa ataaaacaa ttttcgtag 
      ttctgggca ctctcgaaa tcttatcag acgaagacg 
      acgaaagcc ccatcgtca ccctaactt cagggacgt 
      gtctgggcg aaagctccc taattttga gcctcccag 
      ctaagtgtt cagttcgca cgtctagct acacagaat 
      tacaagttc tgtaccgaa gggatttcg ctacaattc 
      tggacgtgc accccatca tttacaaca atatacgcg 
      aggctattt tgcagagaa ttgtcgact cccaagagg 
      tcaaaatgg ttgaggacg aacaaatca acttgcatc 
      cgttcttcc ccaaaagtg acaaagttt cggtgaagg 
      atttgaaag acaagttgc gaccaaaag tcagatttg 
      gggaaaatc gttacgtga ctttgtatc gcttggagg 
      attcggcca aaaaaaatc aaaaatttc cgtggatcc 
      tccaaccgg agtctccag tcaggcagt cttgggagt 
      atttgattt tgctcgtgg ctttttacg ttaacatca 
      cttatcctt actacatta tcttcgtca agtcttacc 
      cgatttggt tcagttttt tctcaaatt tcattgact 
      taatccttg tcttggacc tcgtggtta tcctgacca 
      cggcaattg aggaaagga acgaaaaaa tcgacactt 
      tcatgtcca ttacgcaaa aacgggtga ttcaacccg 
      ggttctact tggactttt tcagttgcg tcaaaaccg 
      agttgtttt actggattt ggacacgaa acgagcgat 
      ttggcgacg ttttaaaac tgaaccgtt tggtcaaaa 
      tttatcgaa tcccgaaac ttatccgga agtacgaaa 
      acaagatcg aggcaaatt ttcacggtt tgactctgc 
      tgaagcatc ttttgtacc ttctggtgc taacttatt 
      ggttcggtg gttgcctgt accttgtga cctgtttcg 
      ataattcgt gtactgaag acggttggt ttttcacga 
      aactgttga aagacttca aggagcaac aaacgagtt 
      acattactg atgattcct tgttaactt ctttgtact 
      tttcgctgt catgcttga ccggtttta cgcctggtg 
      acatcacac cggttaact taaccgatc gggttataa 
      cctgttttg ggtttattt cgggcctct ttatgttta 
      gctgttgtg tggcgcaat tcgccgctg ctttggcaa 
      ggaaaataa aaatgcggt tggacttcc aagagaggt 
      tttgcccag ttttggaaa atctcaaag agagaaact 
      tgagtactc atttggtta agagagtca aaaactatt 
      ttttggtcc agtggatgt atgacaaag cctcgattt 
      tccgccaac acttatttt acaatctcg acccaaaca 
      tccaaaaag tcgtacaag ttgaggaga aaatggaca 
      cgattttgt caatccctt tatttcgaa aattggata 
      aaaatttct tggtggctc ttgtgacta aaacaccgg 
      ttctggtcc taccacccg aagagttca taacgtaca 
      agggcgaat ttttaaaag tgctctaca tagtaatga 
      gaggacact gcattcggc agtttgaaa aaacctcgg 
      tcttgttgg gccctaagt cattcttcg gcactttgg 
      cgttcttct cagacgtta attccctgt gtactgtta 
      aaacagtaa ctcacgtta agcttctct acatcactg 
      aagaaattt tctgggccg cagcttcaa tgtttccaa 
      gagttgcaa aacggttcg gtgcaagtt cggaaaatt 
      ttgacttga tcactgaat ttcgaaaaa taccgatac 
      gtggctcgc ttgataata atttggaac tcgataacg 
      agctctggt tgtccaaag attcacttt tgagcgtta 
      tttcatatt gtaaggagc gtaaacaga gttatagcg 
      cgccttttc gacaactgg gagtcttgt gttgggtcc 
      aaatgtttt tgttaccca tctgtattc ttccatatg 
      ggcgattaa tcaaagttt gggtcaccg gacgactgt 
      tagtgaccc taactgtgg caattttga taccgtacc 
      aattgattc taagcccct acacttcta ccattgata 
      cagtcattt tcgtggccg atttaattg catatttgg 
      acctctatt tcaatttat ttgtcggtt attacggtc 
      ctaaaaatc aactcataa tacatccct tgaaaacag 
      cgacgcatt acttgaaag gaaagtttt tgctagact 
      ttgtgcttt gttttccgt gggaaatgc tagtgaaac 
      acttttctg gaaattatt acaaagtcg ccaaaatca 
      ttcgacttg ggcagatct accgactat tccaatttt 
      ttaactatg tgtccacat ttaccaaaa agaataaat 
      acggagttc ttctccata ttttgaaat ttattccac 
      tggtgatta tttttgttc atttctggg ccacaattt 
      tcgtcataa acacttgta attacgccc caaatggga 
      catttttat gccaagaaa actcatgga ttgataggt 
      ccaaatttc aaaatttcg ctcaccctc ttgaagtgt 
      attccactt cgatttatt tctgcacca atgttttga 
      cttttaccg gtttagggg acattacac cgaaatacc 
      ccagctctg tgattatca caatcgtga tgcagattt 
      gcggcattt tgttcatcg gttatgtgt atgcaaaaa 
      tcgcgagct ataagacgc actggaaca gcattctct 
      tcaaaaccc acgaaacga tgaacgagt tgaagaaat 
      ttatggtcc aggaaaacg ttacgaaaa atctccaaa 
      tgcaaatcg tccagcaat tggaatatc gctggaagc 
      gcaacaaac tcgagccaa acacggctt tggccaaat 
      tccacccaa atctttacg aggattgcg tttacgcaa 
      tgtttgaca gtattttcg aaagcgtcc cggtttttt 
      caaacatcg ataagtcgt ttctgagac ttttgggca 
      cgattgtga aagagcagt gttttcgtc accacgagg 
      tgatttttc gagccgatg atatcgtgt cagagttgt 
      ttatacttg agagaggga ctatcaaaa ttgttgaaa 
      aacttggag gaatggaat tgcacttgg aagccggcg 
      cgatttttg gcaatttga caccaaggg ctttgcata 
      aagtttcaa aaactgtga cagctttga gtaatgttg 
      atgtcctgt gtattaaca cagaagagt tctatttgg 
      cactagata agtttcctt ggattcaaa atgcgattg 
      aggcgaatc tcacaaacg aagagttta tgttacacg 
      agattagaa acgacctct ctgagacaa gtcttacaa 
      aatcaaacc acgattctg ctacaagtt tcaacattt 
      taaaaacct catcggaaa tcaaattaa atttcgtcg 
      ggtgttttc tgttgtgcc tcccttgga aatttttca 
      gtattacaa ttccagaat actccctct ttattaaaa 
      tattcgatg tctactgcc tgttcctag ctcacgtca 
      attcacttc tcgttttgt acgaattct cgttccaaa 
      atcataata attttttgt taggtgtag ccatctaca 
      ctgatgtta cttttattt tgaaaatat taatcgaaa 
      tgcacaaaa cttacgaga acaggtttg gagaacaca 
      ttacttcga acaagaaaa ttgtctcgc gatacttcc 
      gtaacaaga agctgaaac gcgctgttg acatttttg 
      tgaattttc cgttttatt atttaactt ttttcgctg 
      ctgtattta ctagttttg acgcaaatt tttattttt 
      catgtctca gacttatca ccctttaca gattgattt 
      atttttggg agtactaca agagtaaag ccagtcagt 
      tgttagtca aaatttcgc ttctcaaaa tcggtgctt 
      tgattgttt gggggacgt tggtgattc atatttttt 
      catgcgggt ggtatctat gtgcgtgtc ctttaatga 
      cttgcttta aagattctt gggttaatg aggtcttta 
      acacaacct cgccacact tttcgaaaa aactagatc 
      tttatgtta cttcgttat atttcgtga ttactgtaa 
      tgacaacga cgggaactg gcgatattt ccgccaaca 
      gcatccgtg agcaagttt tcatcacgc ttatgatgc 
      taactgcgg tttttctca ctggaattt tcatcggtg 
      agatttgga attgtctca attccacta caatcgcac 
      gattaaaat acgacaacg aaatcaacg agcttaaac 
      agtatttta aaaacagca acgtgagtg attaccaaa 
      tgaaaaaaa tgtggcgct acgtccaac aaatctgga 
      cagtcagtc gaggaaatc aagtaccac gcgcactct 
      cccttttac attcttctg ctttacaag aacttatgt 
      tacaaattt acggacctc atatttcaa actcgtacg 
      tgttttcgg aggtcgaaa agtcattca cccgccagt 
      tatgccaat atctcaagc gtcgtgtgt atttcccgg 
      ggaattaca tagtccaac atggcgatt tcgacggca 
      ccatgtatt tcatccatt atggagaag tcgaaatac 
      tgaccgtac atatgaatt taacagaaa ctatacatg 
      aggtgttac gtagaggag aaatgtttg gagtgatgc 
      agggtttat tttacgata ttccgcacc atttctttt 
      ttcgcgccc gcactgctg tccatttag tggctttgc 
      agtttgatg actggcgac acttactgg agtttttcc 
      ccgaaagtc gggacgcca ttttcgcaa aaacgaaca 
      gagttcaag tgtcaactg tcaaaaaaa taaccaaac 
      atttggttt tttacgaaa aattcaaaa cctattcta 
      caatgagta ctgtgggcc ccttcagac tattaacat 
      atactcgtc agcaagttt tacggtttc agttaaact 
      atcctcgat gtaattacg cgaaaaaag aaaccggaa 
      gcgtcagta tgattggag gggctctga agaagaaaa 
      agttgggga agatgggtc cagtgatgg aaaagaagg 
      tcattccag ttgaaacag accctcaga aacttgtaa 
      aatttgttt gtggctcga atatttaca ctactggtc 
      aagacattg aattaaagc ccgataatg agtatcctg 
      aatggttgt ggaatatac gaacagggc ccccgccgc 
      cactggaag aactagacc caaatacta aggaatatt 
      ggagaagga taaagaaaa tggcaatga gacgaaata 
      ataaattag cacaactta agaagtttt aattagaga 
      aaagaaata aacgagtct agcttaaac ccagctttt 
      gttgtagtt ttccttaca taatcaatc accacaaat 
      tggttttct gagtccaat ttttcacca attattttg 
      taatctaca tataaatca acagttttt ttcaaattt 
      cctacttgg tggcgacag aaaattaaa actaaattt 
      agattctta acaacgctt tgtaaaaca ctgaatata 
      tttctagaa aacagttca taactcaaa tgtacgtta 
      ttctgtttg gccgtccag aactgggac gtaatatca 
      tcaacaatt aaaagacta caatagttt ttatcctga 
      atcaatttc tctaaattc ctcaaagtg gtctcaaac 
      tgttcacca ttgtaacag ttcaaaaaa gtaactaga 
      gttagcgct ttttcaatc acacttttc ctgttcttt 
      actttaaca tttttgaga tgggcatca aggacagtt 
      gtgagcttt aacacactt atgtggaaa gtagtgttt 
      atgaaaaca acacccgat tctttccag ttttgatca 
      agttccttt ggcaaaata caccggact aagaagtca 
      acaagcaag tctttgcta aagtctcct gtcacctcg 
      ataatgtca ttggggcga gggcagcct ccttgaaac 
      aatttggga ttaacgtct tccaaacta aattggaag 
      ttcttcagt cttgaaaat aattgcact cctgactca 
      accaccggt gcctgcatt gtctagaaa ttaattaaa 
      gaatctcag tcatattta tgaccaaaa ataccattc 
      ttagaagaa cgattttca aacaatttg ggtggatgg 
      cgtgcgcgc caattacac caagcgcct tttccagta 
      taagttagt ttgttattt caatataga atttaacag 
      tta
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