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Gene information
General information
TC032140
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000550
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9919484..9922376
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032140
    iBeetle screen iB_09644

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    yolk late amount decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late amount increased - Number of animals showing the phenotype: 1

    mature egg not present - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032140 RA
      MRRSVVPDM IITACLLLL VKIVPIFSK LNGEGKVCG 
      RAIGTNLPE EKIVAGYDV GYYKYPWYA ALIISNVVA 
      CGGTLVGSR MIISAAHCY KEYITGHDA KKTKLEDVY 
      NVTLGIYNR CEKEKTQRV FPVEKVHIH EKYKENIPY 
      FDISLLILK ENANYEPIC LPKSVIKTR PREGSVPGL 
      GTLRYHGST PCTLHEARL LVYSDYECK KMLNRTGND 
      PNKLIGAFC AGYLQGGID TCQGDSGGP FQIMDEFGN 
      HILLGVVSF GFHCANPNL LGVYTDVSH YVNWIEEKS 
      GIKTTMEAS NSTSDESTE PAKPPPRPN PVQVVHQNY 
      PNPGNVIII IRNRNKIED KRQVFIFDN MLVRWVFIV 
      LLYAQADFK CVPNKIKIW SPIKCEAGR RFFAKLGGM 
      PEFGSQISQ PYLYPWLAE IKVSNLGRV RPCSGALIN 
      DKYVLTSQS CVSAPVQAN QINVHLGFY NGYKQDVSE 
      IFQPRVFKS GVSEIIRHF YYDPNNIRT AENNLALLK 
      LNKDVKYSK TIYPIQLPA PGDKFNKYS KTLGENVEW 
      SFFSTNNKK IKDIETKIL DVEQCRQFN PNYHQIGSG 
      VVCGIGDNG SKPCGAEIG GLLSVTKPK KMYNVSVLI 
      GITAWEPPA ANPCQSHLP RVYVRIDNK LAWIKENTR 
      DGSYCRY
    CDS sequence
    • >TC032140 RA
      atgaggcga tcagttgtg cccgacatg atcatcact 
      gcctgtctg ttactgctt gtaaaaata gtaccgatt 
      ttttcgaaa ctaaatggc gagggcaaa gtttgtggt 
      cgagcgatt ggtacaaac ctaccagag gaaaaaata 
      gtggccggg tacgacgtg gggtactac aagtacccc 
      tggtacgct gctttgatt atctcaaat gtggtcgct 
      tgcgggggc actttggtg ggttcaaga atgataatc 
      tcggccgct cattgttac aaagagtat ataacaggg 
      cacgacgct aaaaaaact aagctggaa gacgtctac 
      aatgtcact ctcgggatt tacaaccgg tgcgaaaaa 
      gagaaaact caaagagta tttccggtc gaaaaagtc 
      cacattcat gagaaatac aaagaaaat atcccctac 
      tttgacata tcgttgctc attttaaaa gaaaatgcg 
      aattatgaa ccgatttgt ctcccgaaa agtgtgatc 
      aagacgcgc cctcgggag ggttcagtc ccaggtctt 
      ggcactttg cgttatcac ggatcgact ccttgcacc 
      ctccatgag gcccgactg ctggtctac tccgattac 
      gagtgcaaa aaaatgtta aatcggact gggaatgac 
      ccgaataag ttaatcggc gcgttctgt gctgggtac 
      ttgcaagga gggatcgac acgtgtcaa ggggacagc 
      ggagggcca tttcaaata atggacgag tttggaaat 
      cacattttg ttaggggtg gtttccttt ggcttccac 
      tgcgcgaat ccgaaccta ttaggtgtt tataccgac 
      gtttcgcat tacgttaat tggattgaa gagaaaagt 
      ggaattaaa acaacaatg gaagcgtcg aattcgacc 
      agcgacgaa agcaccgaa ccggctaaa ccaccgcca 
      agacctaac cccgtccaa gtggtccac caaaattac 
      ccaaacccg ggtaatgtt atcataatt atcagaaat 
      cgcaacaaa atcgaagac aagagacaa gtttttata 
      ttcgataac atgttggtg cggtgggtt tttattgtc 
      ctcctttat gcacaagct gatttcaaa tgtgtccca 
      aataagata aaaatctgg tcgcccatc aagtgcgag 
      gccggcaga cgttttttt gcgaaactg ggcggaatg 
      cccgaattt gggagccaa atttcgcag ccgtatttg 
      tacccctgg ctggctgaa atcaaggtc tcgaacttg 
      ggccgggtc cgtccctgt tcgggggcg ttgattaac 
      gataaatat gttttaact tcgcagagt tgcgtttca 
      gccccagtt caagcaaat caaataaat gttcatctc 
      gggttctat aatgggtat aagcaggac gtgtcggag 
      attttccag ccaagggtt ttcaaaagt ggggtcagc 
      gaaattatc aggcatttt tactacgat cccaataat 
      atcagaacg gcggaaaat aatttagcg ttgctcaaa 
      ttgaataaa gatgtcaag tatagtaaa accatttat 
      ccgatccaa ttaccggca cctggcgac aagttcaac 
      aaatattca aagactctt ggagaaaac gtcgagtgg 
      tcatttttt tcaaccaat aataagaaa ataaaagac 
      attgaaaca aaaatattg gacgtggaa caatgtaga 
      caatttaat cccaattat catcaaatt ggaagtgga 
      gttgtttgt ggtatcggt gataatggc tctaaacca 
      tgtggggcg gaaattggg ggcttactc agtgttaca 
      aaaccgaaa aaaatgtat aatgtctct gttttaata 
      ggtataaca gcgtgggaa cccccggct gcaaaccct 
      tgccagagt catttacct cgagtttac gtccgcata 
      gacaacaaa ctcgcctgg atcaaagaa aacacacgc 
      gatggttct tattgtcgt tactaa
    mRNA sequence
    • >TC032140 RA
      ataattgct tttccacac tagaaaaat acagaaaat 
      tcgcccaag gtttcaaat tcttgcata attgggaac 
      attccgatt gataaaatt tcaaatgag gcgatcagt 
      tgtgcccga catgatcat cactgcctg tctgttact 
      gcttgtaaa aatagtacc gattttttc gaaactaaa 
      tggcgaggg caaagtttg tggtcgagc gattggtac 
      aaacctacc agaggaaaa aatagtggc cgggtacga 
      cgtggggta ctacaagta cccctggta cgctgcttt 
      gattatctc aaatgtggt cgcttgcgg gggcacttt 
      ggtgggttc aagaatgat aatctcggc cgctcattg 
      ttacaaaga gtatataac agggcacga cgctaaaaa 
      aactaagct ggaagacgt ctacaatgt cactctcgg 
      gatttacaa ccggtgcga aaaagagaa aactcaaag 
      agtatttcc ggtcgaaaa agtccacat tcatgagaa 
      atacaaaga aaatatccc ctactttga catatcgtt 
      gctcatttt aaaagaaaa tgcgaatta tgaaccgat 
      ttgtctccc gaaaagtgt gatcaagac gcgccctcg 
      ggagggttc agtcccagg tcttggcac tttgcgtta 
      tcacggatc gactccttg caccctcca tgaggcccg 
      actgctggt ctactccga ttacgagtg caaaaaaat 
      gttaaatcg gactgggaa tgacccgaa taagttaat 
      cggcgcgtt ctgtgctgg gtacttgca aggagggat 
      cgacacgtg tcaagggga cagcggagg gccatttca 
      aataatgga cgagtttgg aaatcacat tttgttagg 
      ggtggtttc ctttggctt ccactgcgc gaatccgaa 
      cctattagg tgtttatac cgacgtttc gcattacgt 
      taattggat tgaagagaa aagtggaat taaaacaac 
      aatggaagc gtcgaattc gaccagcga cgaaagcac 
      cgaaccggc taaaccacc gccaagacc taaccccgt 
      ccaagtggt ccaccaaaa ttacccaaa cccgggtaa 
      tgttatcat aattatcag aaatcgcaa caaaatcga 
      agacaagag acaagtttt tatattcga taacatgtt 
      ggtgcggtg ggtttttat tgtcctcct ttatgcaca 
      agctgattt caaatgtgt cccaaataa gataaaaat 
      ctggtcgcc catcaagtg cgaggccgg cagacgttt 
      ttttgcgaa actgggcgg aatgcccga atttgggag 
      ccaaatttc gcagccgta tttgtaccc ctggctggc 
      tgaaatcaa ggtctcgaa cttgggccg ggtccgtcc 
      ctgttcggg ggcgttgat taacgataa atatgtttt 
      aacttcgca gagttgcgt ttcagcccc agttcaagc 
      aaatcaaat aaatgttca tctcgggtt ctataatgg 
      gtataagca ggacgtgtc ggagatttt ccagccaag 
      ggttttcaa aagtggggt cagcgaaat tatcaggca 
      tttttacta cgatcccaa taatatcag aacggcgga 
      aaataattt agcgttgct caaattgaa taaagatgt 
      caagtatag taaaaccat ttatccgat ccaattacc 
      ggcacctgg cgacaagtt caacaaata ttcaaagac 
      tcttggaga aaacgtcga gtggtcatt tttttcaac 
      caataataa gaaaataaa agacattga aacaaaaat 
      attggacgt ggaacaatg tagacaatt taatcccaa 
      ttatcatca aattggaag tggagttgt ttgtggtat 
      cggtgataa tggctctaa accatgtgg ggcggaaat 
      tgggggctt actcagtgt tacaaaacc gaaaaaaat 
      gtataatgt ctctgtttt aataggtat aacagcgtg 
      ggaaccccc ggctgcaaa cccttgcca gagtcattt 
      acctcgagt ttacgtccg catagacaa caaactcgc 
      ctggatcaa agaaaacac acgcgatgg ttcttattg 
      tcgttacta ataaaacac caatagata tgaaaaaat 
      ttattaaat aacgaataa atta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY