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Gene information
General information
TC000607 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9098560..9101159
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000607
    iBeetle screen iB_09641

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000607 RA
      MGVANETKR LDLKINGLD VGQEHDTRK IWLNFEFDI 
      SGDEAVSQF LIFDNCGLA TLRISFEKL EKFRLFREE 
      PRESFRFYF DRKQVVILP GMRAEIPIW FKTDRPGTY 
      SENWEIRTQ PALWSDDFR VILVLNAYS CFKNLQQKI 
      DTTVKLIEI KARNLLIKN LLDDCLKTI QYKETPPKV 
      CVYGEKELF ELANFSMGR PQFIYDEKL VKELNQIYD 
      RVKSGEEKW DFSIRSLTK LAHRKDTII YLDNKMLEL 
      VEHILKIEK GAQEPPKKI ETKVKEKKQ KKKKDKKSD 
      KKTKSSKSS TSQPVEETK PVVKPEPFE YSNQAQVWD 
      VVRKFDSPV IINTQREKY LTCHSILSA YFGKMCHSL 
      NELELEIGL NSTRTYPRV KNRLLPTNE LPERLVYEY 
      FEHHYIPTV PLREVYPYD AKKKPKVLR HVPSDDAEA 
      LYTIYFNKT IGEVDKKKK GKGGKEKKK GKEDKEKKK 
      GKGDKEKKK KDKGDKHKG KDKGKKKKK VESRENIKK 
      EISIESFNP YKEQFVYFS ESEDEVVDN AGVKKRDLS 
      KEELNDYKY KQYLIVYSS LGNAVDALV DTLESFKYV 
      VSYPTLSEV KSQDEKH
    CDS sequence
    • >TC000607 RA
      atgggagtt gcaaacgag acgaaacgt ctcgatttg 
      aaaattaac gggctcgat gtgggacaa gaacacgac 
      acgaggaaa atttggctg aatttcgag tttgatatt 
      tcgggtgat gaggcagtt tcccagttt ctaatcttt 
      gataattgc ggtttggca acattgaga attagtttt 
      gaaaaactg gaaaagttt cgtttgttt cgggaagag 
      ccgagggaa agtttcagg ttttatttt gaccggaaa 
      caagtcgtg attttgccc ggaatgagg gcggaaatc 
      ccgatttgg ttcaaaacg gaccggcca gggacgtat 
      tcggaaaat tgggaaatt cgcactcaa ccggccctg 
      tggagcgac gatttccgc gtaattttg gtgttaaat 
      gcgtactcg tgtttcaaa aacttgcaa caaaaaatt 
      gacacgacc gtgaaatta attgaaatc aaagctagg 
      aatttacta attaaaaat ctcttggat gattgcttg 
      aaaacgatc cagtataag gagactccg cctaaagtt 
      tgcgtttac ggagagaaa gaattattc gaacttgcg 
      aatttttca atgggacga ccacaattc atctacgac 
      gaaaaactt gtaaaagaa ctgaaccaa atttacgat 
      cgagtcaag tcgggagaa gagaagtgg gacttttcc 
      attcgtagt ttaacaaag ttagcacat cgtaaagat 
      acgattatt taccttgat aataaaatg ctagaactg 
      gttgaacac atcttaaaa attgaaaag ggggctcag 
      gaacccccc aaaaaaatt gaaaccaaa gtaaaggaa 
      aagaaacag aagaaaaag aaagacaag aaatcagac 
      aaaaaaaca aaatcaagt aaatcatcg acgtcgcaa 
      cccgtcgaa gaaaccaaa ccggtggtt aaacccgaa 
      ccgttcgaa tattcaaac caagcccag gtttgggac 
      gtggtccga aaattcgat tcccccgtc attattaac 
      acacaaagg gagaaatac ttgacgtgt cactctatt 
      ctgagtgcc tattttggt aaaatgtgc cactcgtta 
      aacgaactt gaacttgaa attggtttg aactcaact 
      agaacttat ccacgcgtc aaaaacagg cttttgccg 
      accaatgaa cttccggag agattggtc tatgagtat 
      tttgagcat cattatatt cctactgtt ccgctaagg 
      gaggtttat ccatacgat gcgaaaaaa aagccgaag 
      gttttgaga catgtgccg agtgacgac gctgaggcg 
      ttgtatacg atttatttt aacaaaact attggtgaa 
      gtagacaag aagaagaaa ggtaaaggc ggtaaagaa 
      aagaagaaa ggtaaagag gataaagaa aagaagaaa 
      ggtaaaggt gataaagag aagaaaaag aaagataaa 
      ggtgataaa cacaagggt aaggataaa gggaagaaa 
      aagaagaag gtcgagtcg agggagaac ataaaaaag 
      gagatttca atcgaaagt ttcaatccg tataaagaa 
      cagtttgtc tatttttcg gaaagtgag gacgaagtg 
      gtcgacaat gcgggagtc aagaaaagg gatttgtcg 
      aaagaagag ttgaatgat tataaatat aaacaatat 
      ctcattgtt tactcctct ttggggaat gctgttgat 
      gcccttgtt gacacttta gagtcgttt aaatatgtc 
      gtttcgtac ccgacgttg agcgaagtt aaatcgcaa 
      gacgaaaaa cattga
    mRNA sequence
    • >TC000607 RA
      cgaggaaaa acaagccct cgacagcgc aaaaatccc 
      gacggaatt cgacaaata ccggggaaa gcccgactt 
      ttggcacct cccttttgc cctcccccg gaaacaccc 
      ctgttgtcc gtgcgaacc tgattattt cgccgtgaa 
      aacgaaaca agaggggta cgaaatacc ccccatcga 
      gcacaaagg ggtaccgac tgtaattcg aaacgaaaa 
      aaatatttt aaacgggac gcgcaacat ttataagcg 
      ctggcaaaa tagccaata ccgttacga gcaatttat 
      taaatgggc ggagaaagt cgcgcacat cgtcccgca 
      ttggcccga tttgcaaca cttggttgt gattggctc 
      aaaaattgc aaacgagga gagtgggga tcatgcgga 
      cgtagcgag tgaaacgac aaaagtgag cgaagttgg 
      aatagaaga acccatgat gggagttgc aaacgagac 
      gaaacgtct cgatttgaa aattaacgg gctcgatgt 
      gggacaaga acacgacac gaggaaaat ttggctgaa 
      tttcgagtt tgatatttc gggtgatga ggcagtttc 
      ccagtttct aatctttga taattgcgg tttggcaac 
      attgagaat tagttttga aaaactgga aaagtttcg 
      tttgtttcg ggaagagcc gagggaaag tttcaggtt 
      ttattttga ccggaaaca agtcgtgat tttgcccgg 
      aatgagggc ggaaatccc gatttggtt caaaacgga 
      ccggccagg gacgtattc ggaaaattg ggaaattcg 
      cactcaacc ggccctgtg gagcgacga tttccgcgt 
      aattttggt gttaaatgc gtactcgtg tttcaaaaa 
      cttgcaaca aaaaattga cacgaccgt gaaattaat 
      tgaaatcaa agctaggaa tttactaat taaaaatct 
      cttggatga ttgcttgaa aacgatcca gtataagga 
      gactccgcc taaagtttg cgtttacgg agagaaaga 
      attattcga acttgcgaa tttttcaat gggacgacc 
      acaattcat ctacgacga aaaacttgt aaaagaact 
      gaaccaaat ttacgatcg agtcaagtc gggagaaga 
      gaagtggga cttttccat tcgtagttt aacaaagtt 
      agcacatcg taaagatac gattattta ccttgataa 
      taaaatgct agaactggt tgaacacat cttaaaaat 
      tgaaaaggg ggctcagga accccccaa aaaaattga 
      aaccaaagt aaaggaaaa gaaacagaa gaaaaagaa 
      agacaagaa atcagacaa aaaaacaaa atcaagtaa 
      atcatcgac gtcgcaacc cgtcgaaga aaccaaacc 
      ggtggttaa acccgaacc gttcgaata ttcaaacca 
      agcccaggt ttgggacgt ggtccgaaa attcgattc 
      ccccgtcat tattaacac acaaaggga gaaatactt 
      gacgtgtca ctctattct gagtgccta ttttggtaa 
      aatgtgcca ctcgttaaa cgaacttga acttgaaat 
      tggtttgaa ctcaactag aacttatcc acgcgtcaa 
      aaacaggct tttgccgac caatgaact tccggagag 
      attggtcta tgagtattt tgagcatca ttatattcc 
      tactgttcc gctaaggga ggtttatcc atacgatgc 
      gaaaaaaaa gccgaaggt tttgagaca tgtgccgag 
      tgacgacgc tgaggcgtt gtatacgat ttattttaa 
      caaaactat tggtgaagt agacaagaa gaagaaagg 
      taaaggcgg taaagaaaa gaagaaagg taaagagga 
      taaagaaaa gaagaaagg taaaggtga taaagagaa 
      gaaaaagaa agataaagg tgataaaca caagggtaa 
      ggataaagg gaagaaaaa gaagaaggt cgagtcgag 
      ggagaacat aaaaaagga gatttcaat cgaaagttt 
      caatccgta taaagaaca gtttgtcta tttttcgga 
      aagtgagga cgaagtggt cgacaatgc gggagtcaa 
      gaaaaggga tttgtcgaa agaagagtt gaatgatta 
      taaatataa acaatatct cattgttta ctcctcttt 
      ggggaatgc tgttgatgc ccttgttga cactttaga 
      gtcgtttaa atatgtcgt ttcgtaccc gacgttgag 
      cgaagttaa atcgcaaga cgaaaaaca ttgaattaa 
      taaaaacgc ttaatgaaa actattatt gactttttc 
      ctctcctga cctgtcaac tcatttgca tttttcaat 
      tatttatca cttatctag tccaatatc aatactcct 
      ttccatgaa ttcgatttt tgtacgttt tatcctctc 
      cacccatac agagtgttg tctttttgg gacacattt 
      accaccatc ataaatctt tagcacaaa tttgtttgg 
      tctcaatac gaacgacgg ccagaaagt ttggaaaaa 
      tgcaagcga aaggttcag gtgagtcgg tgaatattt 
      cattacgct gttttattg attcatgca ttgtttcaa 
      ttgtcgtaa aaatttgat tttttgaat aaatttatt 
      acaaggtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY