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Gene information
General information
TC000976 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9092691..9093798
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000976
    iBeetle screen iB_09640

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva fragmented - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000976 RA
      MLENYLIEI CIILVLLVI LYFFVGWFC RCNTNEKML 
      CTYGYSDSD GPNTWKYRF QIRGENQSP INILSVCAV 
      VVPTETIAP LDFTSEYHI TPQEMKLLN DGYTVVIYS 
      TWSNGRKPT VCGGPLCDE YKLFSIRFR WGPNDEEGS 
      EHMIDMRRY AMELQVTYN KCSCKCTDM AKSIKTCAV 
      IIISYMFEV TDVDNPYLE TIIQGLRHI QNPCCSYYI 
      RPVPVSLLA PVFTRRYFS YIGSLTYPP CTEGVRWII 
      QPEPLAISS HQIRQFRRL MGFNGYILN NTRPVQNAN 
      NRDVIFYE
    CDS sequence
    • >TC000976 RA
      atgttggaa aattatttg atcgaaatt tgcataatc 
      ctcgtcctc ttggtcata ttgtacttt ttcgtgggt 
      tggttttgc cgctgcaac accaacgaa aaaatgctc 
      tgcacctac ggctacagc gattccgac ggccccaac 
      acttggaaa taccgcttc caaatccgt ggcgagaac 
      caatccccc atcaacatc ctctcagtc tgcgccgtc 
      gttgtcccc accgaaacc atcgcccct ttggacttc 
      accagcgag taccacatc accccccag gagatgaag 
      ctgctcaac gacggctac accgttgtg atttacagc 
      acttggtcc aacgggcgc aaacccacc gtttgtggg 
      ggccccttg tgcgacgaa tacaagctg tttagtatc 
      cgcttccgg tgggggccc aacgacgag gagggctcc 
      gagcacatg atcgacatg cggcgctac gccatggag 
      ctccaagtc acttacaac aaatgctcg tgcaagtgc 
      acggatatg gccaagtcg atcaaaact tgcgctgtg 
      atcatcatt agttacatg tttgaagtg actgatgtc 
      gacaatccc tatcttgag acaataata caggggttg 
      cgacacata caaaacccc tgttgcagt tattacatc 
      aggccggtt ccggtcagt ttgttagct ccggttttt 
      acgcgacga tatttttct tatattggc tccctgacc 
      tatcctccg tgtactgag ggcgttcgc tggattatt 
      cagccggag cctttggcc atttcctcg caccagata 
      agacagttt aggcggctg atgggcttc aatggttat 
      atcttgaat aatacgcga cccgttcaa aatgccaac 
      aacagagac gtgattttc tatgaataa
    mRNA sequence
    • >TC000976 RA
      tgaaaggtg tcactcagt ccgtgtcat ttacgttag 
      aaattaaga aaaatgtca gtaattaaa taatcatgt 
      tggaaaatt atttgatcg aaatttgca taatcctcg 
      tcctcttgg tcatattgt actttttcg tgggttggt 
      tttgccgct gcaacacca acgaaaaaa tgctctgca 
      cctacggct acagcgatt ccgacggcc ccaacactt 
      ggaaatacc gcttccaaa tccgtggcg agaaccaat 
      cccccatca acatcctct cagtctgcg ccgtcgttg 
      tccccaccg aaaccatcg cccctttgg acttcacca 
      gcgagtacc acatcaccc cccaggaga tgaagctgc 
      tcaacgacg gctacaccg ttgtgattt acagcactt 
      ggtccaacg ggcgcaaac ccaccgttt gtgggggcc 
      ccttgtgcg acgaataca agctgttta gtatccgct 
      tccggtggg ggcccaacg acgaggagg gctccgagc 
      acatgatcg acatgcggc gctacgcca tggagctcc 
      aagtcactt acaacaaat gctcgtgca agtgcacgg 
      atatggcca agtcgatca aaacttgcg ctgtgatca 
      tcattagtt acatgtttg aagtgactg atgtcgaca 
      atccctatc ttgagacaa taatacagg ggttgcgac 
      acatacaaa acccctgtt gcagttatt acatcaggc 
      cggttccgg tcagtttgt tagctccgg tttttacgc 
      gacgatatt tttcttata ttggctccc tgacctatc 
      ctccgtgta ctgagggcg ttcgctgga ttattcagc 
      cggagcctt tggccattt cctcgcacc agataagac 
      agtttaggc ggctgatgg gcttcaatg gttatatct 
      tgaataata cgcgacccg ttcaaaatg ccaacaaca 
      gagacgtga ttttctatg aataaaatg taggggttt 
      ggtcaaaat tgtctcgtg gttgtttca ataaattat 
      gcatggtgg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY