Status
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Gene information
General information
TC000974 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9083621..9086682
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000974
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09639

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 3
    yolk sometimes amount decreased - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000974 RA
      MSLFPAYAG ESAPPEQPP HTPQWLQNP SFSIDVTGA 
      PEQPPEEEP PQADDSPEP KKRKKKHKH KDKPVVDDN 
      KLEEEGFIL DTVRTKEFL TVKTIARPS APRYSVRYY 
      VTGARTARK RKKIKRYYH WSFLLEDKK EGEEEVITE 
      KNRDSLEGS KKDENFAGF KQEEDLSQT TASFNKKLA 
      ESPHDIKLW LEYVNFQDM VYQFEKTYR KGSIAKGLR 
      VLAERKLAI LDKALTHNQ NCEELLRER LNVAVSIYP 
      SDELQVQLK NLVDKDQGN IILWQGYIE SIQCSMSHC 
      NTPSVLDLY TKCLSILHK LRRNSSMEK AQLEENILK 
      MLYQCGLFL KQAGLFEQL WTLLRLYLE LNLSPTDKN 
      KFNIGSGFD EKQLVELEE VVFNSQLPL HELWLRTEK 
      LREACHWLP WMEDGECED PQRLVFPDD VAELIHPIT 
      MPENMFRLI ATILSLLKI PILFCRHST MQELGLDYV 
      PWALDSIES LIGIFLPLY PIDLTNKNL VIDSRLSVG 
      PQYLKVLPG QEEYLSFVL TTMKSCTEC LSGDDKIAM 
      TVWWLRFQR LLIILEKQG RFKMPQGLA KKIKSNVKN 
      LLKQEENRS NTIFYCEYA LIEFELGNP QTCLNIIQT 
      ALSFHKPIL AEKASRCHL HRTLVEVCL SQGKDSDAL 
      QHLICYALE KTNIESIND DLLNQTTLK FKHTTLQLL 
      QTELEKLPV ANQFLPNFF TDWIICNGW FLYLTKGAL 
      QCGTFLENI LADLDEKHE GMLWQKEVL FEFYVAVLF 
      KHCVANPGF GVFKILDDV LTRAVELYP NNLFLLAVL 
      AKEQSVTCC AGSQWWKLK SLLMKTGRA FPILFLVLI 
      ANQQMVETQ EKYIETFSG RKYEVSDSH KNRMLALFR 
      HITRPEMCT RRCGLVWRL YLQFVHAYF DPNLCRNVY 
      FCAVEECPW LKALYIDAA IYIPAELAQ IQDLLIEKQ 
      LRIHVTPEE LDVLRS
    CDS sequence
    • >TC000974 RA
      atgtcgctc ttcccggct tacgctggt gaaagtgcc 
      cccccagag cagcccccg cacaccccc cagtggctc 
      caaaacccc agcttcagt atcgacgtg accggagcg 
      ccggagcag ccccctgaa gaggaaccg ccacaagct 
      gatgacagt cccgagcca aaaaaacgc aaaaagaag 
      cataaacat aaagacaaa ccggttgtt gatgacaat 
      aaattagag gaagagggt tttattctt gatacagtt 
      cggactaaa gaattttta actgtgaaa acaatcgcc 
      cggccctcg gccccgcgt tactcggtc cggtattac 
      gtaacgggg gctcggacg gcccgtaaa cgtaaaaaa 
      atcaaacgg tattaccac tggtcattt ctccttgaa 
      gataaaaag gaaggggaa gaggaagtc ataactgaa 
      aaaaatcgt gactcgctt gagggaagc aaaaaagat 
      gagaatttc gcggggttt aagcaggaa gaggatttg 
      tcgcaaacc acggcttcg tttaacaaa aagttggcc 
      gagagcccc catgatatt aagttgtgg ctggagtac 
      gtcaatttc caggatatg gtgtaccag tttgaaaaa 
      acctaccgg aaaggaagc attgccaaa ggtttgaga 
      gtgttagct gaacgaaaa ctggcgatt ttggacaag 
      gccttgacc cacaatcaa aattgcgag gaattatta 
      agggagaga ctcaatgtg gccgtcagt atttacccc 
      tctgatgag ttacaagtc cagttgaaa aacctagtt 
      gataaagac caggggaac attattcta tggcaaggc 
      tacattgag tcaatccag tgctccatg tctcactgc 
      aacacccct tcagtcctc gacttgtac acaaagtgt 
      ctctcgatt ttgcacaaa ctgcggcgc aactcatca 
      atggagaag gcacaactc gaggaaaac attttaaaa 
      atgctctac caatgcggt ctatttttg aagcaagcc 
      ggcttgttt gaacaactc tggaccctc ttgcgccta 
      tacctcgaa ctaaatcta tccccaacc gacaagaac 
      aagttcaat atcgggtca ggctttgac gagaaacaa 
      ttggtcgaa ttggaggaa gttgtcttc aactcgcaa 
      ctccccctc catgagttg tggcttcgc acggaaaaa 
      ctgagggag gcgtgccat tggctgcct tggatggaa 
      gacggtgag tgtgaggat ccacaacga ttggtcttc 
      cctgatgat gtagccgag ttgattcac ccaatcaca 
      atgcccgaa aatatgttt cgactaatc gcaacaatc 
      ttatcactt ttgaaaatc ccgatcttg ttttgtcgg 
      catagtacg atgcaggag ttggggttg gattacgtg 
      ccgtgggct ctagattcg atagagtcg ctgattggg 
      atttttttg cccctgtac ccgattgat ttgactaac 
      aagaacctg gtcattgat agtcggtta tcggtgggg 
      ccccaatat ttgaaagtc ttgcccgga caagaggag 
      tatttgagt tttgtccta acgacgatg aaaagttgt 
      accgagtgt ttgagtggg gacgacaaa attgcaatg 
      acagtctgg tggttgcgc ttccagaga ttgctaatt 
      attctggaa aagcaaggg cggtttaaa atgccacaa 
      ggactagcc aaaaaaatc aaatctaac gtcaaaaat 
      ttactcaaa caagaggaa aatcgtagt aacacgatt 
      ttctactgc gagtatgcg ttaattgag tttgaattg 
      gggaatccc caaacttgc ttaaacata atccagact 
      gctttgtcc ttccataag ccaattttg gccgaaaag 
      gcgtctcgg tgtcacttg catcggact ttggtcgaa 
      gtttgttta agtcagggc aaggattcg gacgcttta 
      cagcaccta atttgttac gccctcgaa aaaacgaac 
      attgagtca atcaatgac gacctcctc aaccaaaca 
      accctcaaa tttaaacac acgaccttg cagttgctc 
      caaaccgag ctggaaaaa ctcccagtc gcgaatcaa 
      tttttaccg aattttttc accgattgg atcatttgt 
      aacggttgg tttttgtac ctaactaaa ggggcactc 
      cagtgtggc acattcctt gaaaatatc ttagccgac 
      ttggacgag aaacacgaa gggatgttg tggcaaaag 
      gaggttttg ttcgagttt tacgtcgct gtcctcttc 
      aaacactgt gtggcgaac cccggcttt ggcgttttt 
      aaaatactg gacgatgtt ttaactcgt gctgttgaa 
      ctttaccct aataatctc ttcctgttg gccgttttg 
      gccaaggaa caaagcgtc acgtgttgt gcggggtcg 
      cagtggtgg aaattgaaa agtttgttg atgaaaacg 
      gggcgcgct ttcccgatt ttgttcctc gtgctgatc 
      gcgaaccaa caaatggtt gagacgcag gaaaagtac 
      attgagact ttttcaggt cgtaaatac gaggtgagc 
      gacagtcat aaaaaccgg atgttggcc cttttccgc 
      cacatcacc aggccggag atgtgcacc aggcggtgc 
      gggctagtt tggaggctg tacttgcaa tttgttcac 
      gcgtatttt gacccgaat ttgtgtagg aatgtgtat 
      ttttgcgcc gtcgaagaa tgtccgtgg ttgaaagcg 
      ttgtatatc gatgctgcg atttacatc ccggctgag 
      ttggctcag atccaggac ttgttgatc gagaaacag 
      ctgcgcatt catgtgacg cccgaggag ttggacgtt 
      ttgcgcagt tga
    mRNA sequence
    • >TC000974 RA
      tgacatttg aggttagat tgtttattt acgttcaca 
      aacgtttga aatagtgcg ttatgtcgc tcttcccgg 
      cttacgctg gtgaaagtg cccccccag agcagcccc 
      cgcacaccc cccagtggc tccaaaacc ccagcttca 
      gtatcgacg tgaccggag cgccggagc agccccctg 
      aagaggaac cgccacaag ctgatgaca gtcccgagc 
      caaaaaaac gcaaaaaga agcataaac ataaagaca 
      aaccggttg ttgatgaca ataaattag aggaagagg 
      gttttattc ttgatacag ttcggacta aagaatttt 
      taactgtga aaacaatcg cccggccct cggccccgc 
      gttactcgg tccggtatt acgtaacgg gggctcgga 
      cggcccgta aacgtaaaa aaatcaaac ggtattacc 
      actggtcat ttctccttg aagataaaa aggaagggg 
      aagaggaag tcataactg aaaaaaatc gtgactcgc 
      ttgagggaa gcaaaaaag atgagaatt tcgcggggt 
      ttaagcagg aagaggatt tgtcgcaaa ccacggctt 
      cgtttaaca aaaagttgg ccgagagcc cccatgata 
      ttaagttgt ggctggagt acgtcaatt tccaggata 
      tggtgtacc agtttgaaa aaacctacc ggaaaggaa 
      gcattgcca aaggtttga gagtgttag ctgaacgaa 
      aactggcga ttttggaca aggccttga cccacaatc 
      aaaattgcg aggaattat taagggaga gactcaatg 
      tggccgtca gtatttacc cctctgatg agttacaag 
      tccagttga aaaacctag ttgataaag accagggga 
      acattattc tatggcaag gctacattg agtcaatcc 
      agtgctcca tgtctcact gcaacaccc cttcagtcc 
      tcgacttgt acacaaagt gtctctcga ttttgcaca 
      aactgcggc gcaactcat caatggaga aggcacaac 
      tcgaggaaa acattttaa aaatgctct accaatgcg 
      gtctatttt tgaagcaag ccggcttgt ttgaacaac 
      tctggaccc tcttgcgcc tatacctcg aactaaatc 
      tatccccaa ccgacaaga acaagttca atatcgggt 
      caggctttg acgagaaac aattggtcg aattggagg 
      aagttgtct tcaactcgc aactccccc tccatgagt 
      tgtggcttc gcacggaaa aactgaggg aggcgtgcc 
      attggctgc cttggatgg aagacggtg agtgtgagg 
      atccacaac gattggtct tccctgatg atgtagccg 
      agttgattc acccaatca caatgcccg aaaatatgt 
      ttcgactaa tcgcaacaa tcttatcac ttttgaaaa 
      tcccgatct tgttttgtc ggcatagta cgatgcagg 
      agttggggt tggattacg tgccgtggg ctctagatt 
      cgatagagt cgctgattg ggatttttt tgcccctgt 
      acccgattg atttgacta acaagaacc tggtcattg 
      atagtcggt tatcggtgg ggccccaat atttgaaag 
      tcttgcccg gacaagagg agtatttga gttttgtcc 
      taacgacga tgaaaagtt gtaccgagt gtttgagtg 
      gggacgaca aaattgcaa tgacagtct ggtggttgc 
      gcttccaga gattgctaa ttattctgg aaaagcaag 
      ggcggttta aaatgccac aaggactag ccaaaaaaa 
      tcaaatcta acgtcaaaa atttactca aacaagagg 
      aaaatcgta gtaacacga ttttctact gcgagtatg 
      cgttaattg agtttgaat tggggaatc cccaaactt 
      gcttaaaca taatccaga ctgctttgt ccttccata 
      agccaattt tggccgaaa aggcgtctc ggtgtcact 
      tgcatcgga ctttggtcg aagtttgtt taagtcagg 
      gcaaggatt cggacgctt tacagcacc taatttgtt 
      acgccctcg aaaaaacga acattgagt caatcaatg 
      acgacctcc tcaaccaaa caaccctca aatttaaac 
      acacgacct tgcagttgc tccaaaccg agctggaaa 
      aactcccag tcgcgaatc aatttttac cgaattttt 
      tcaccgatt ggatcattt gtaacggtt ggtttttgt 
      acctaacta aaggggcac tccagtgtg gcacattcc 
      ttgaaaata tcttagccg acttggacg agaaacacg 
      aagggatgt tgtggcaaa aggaggttt tgttcgagt 
      tttacgtcg ctgtcctct tcaaacact gtgtggcga 
      accccggct ttggcgttt ttaaaatac tggacgatg 
      ttttaactc gtgctgttg aactttacc ctaataatc 
      tcttcctgt tggccgttt tggccaagg aacaaagcg 
      tcacgtgtt gtgcggggt cgcagtggt ggaaattga 
      aaagtttgt tgatgaaaa cggggcgcg ctttcccga 
      ttttgttcc tcgtgctga tcgcgaacc aacaaatgg 
      ttgagacgc aggaaaagt acattgaga ctttttcag 
      gtcgtaaat acgaggtga gcgacagtc ataaaaacc 
      ggatgttgg cccttttcc gccacatca ccaggccgg 
      agatgtgca ccaggcggt gcgggctag tttggaggc 
      tgtacttgc aatttgttc acgcgtatt ttgacccga 
      atttgtgta ggaatgtgt atttttgcg ccgtcgaag 
      aatgtccgt ggttgaaag cgttgtata tcgatgctg 
      cgatttaca tcccggctg agttggctc agatccagg 
      acttgttga tcgagaaac agctgcgca ttcatgtga 
      cgcccgagg agttggacg ttttgcgca gttgagggc 
      ctttctgtg atattccat tttatttca cataaatta 
      aacaaaaat aacactc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY