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Gene information
General information
TC000615 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8983103..8986348
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000615
    iBeetle screen iB_09635

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000615 RA
      MPVPLLKDS QMQFGAITF FAKQHKGDT CGSNGLPLT 
      PNSIIIIGR AQILKTIKN PYLCQYLDI VRGKHERTV 
      VVSQYCGTP LSDYITKEP FDYDDVRRF AYQILTALN 
      YLHERKIVH RNLSTENIL LQKNKDIKL FNYGLFYMT 
      GCGQLVSFP IMQILYSAP EIYLDSPTS TQADPKTDI 
      WSLGIVLIE LILNKVLWQ SLKLGQRIR KILSLVQCN 
      TSVFERIAR EHNCYDKYE SVPNDLREI IEACLNIYP 
      QNRPTCHEL LANPVFQSF KSEKTVNKS KICEPFEIF 
      TIRELYHWW QLAGGDVFQ ELKKQGLIR SSPPILSLP 
      NLVTIEGTT VGQERNPAT LYDPRIVQM PLDTLYQRF 
      AHIPLNCFY PLIHSTSKI IESGVPPAY DATGLPLVI 
      RERDPEYQF HRVILFRRL LHGYPHTKD MILKEASKD 
      IPPLLRGEI WAALLDVKG DYERQYLRI DKETSTTTD 
      RQIEVDIPR CHQYNELLS SAEGHKKLK RILKAWVYQ 
      NSNYVYWQG LDSLTAPFL FLNFNNEAK AFACLSAFI 
      PKYLHKFFL KDNSMVIQE YLAKFSQLI AFHDPHLAN 
      HLHEINFYP ELFAIPWFL TVFSHVFPL YKILHLWDK 
      LLLGDSSFP LHIGLSVLT QLRDRLLNS GFNECILLF 
      SDLPEVDIE KCVSYSTAT FQSTPKSIT AREHQNGKY 
      HPKNELDIS NVSLQELNR ERCPRLSAS DFVDLVRLE 
      PEKLLIIDI RNPVQFNRS SVVNSVNIP FSSVTFGEA 
      SLENVGQHS TTIKNSGGK IIVIVGNEE TDLELFPKF 
      LLKCGVSRV CVLHGGFSL LPTITPSIL ISQSNLIN
    CDS sequence
    • >TC000615 RA
      atgccggtg cctcttttg aaagactcc caaatgcag 
      tttggggct atcacattt tttgcaaag caacataaa 
      ggcgatacg tgcgggagt aacggtctt cctctaact 
      ccaaactca ataattata attggaagg gcacaaatt 
      ctcaaaaca atcaaaaat ccgtactta tgccaatat 
      ttagatata gtgaggggc aaacacgaa aggacagtg 
      gtcgtgtcc cagtattgt gggaccccc ttgtctgat 
      tatatcaca aaagagccg ttcgattac gacgacgtt 
      agaagattc gcttatcaa attttgacc gctttgaat 
      tatttacat gaacgcaaa atcgtgcat agaaattta 
      agcacggaa aacatctta ttgcagaaa aacaaagac 
      attaagttg ttcaattac ggccttttt tatatgaca 
      ggatgcgga caattagtt tcattccca ataatgcaa 
      atattatat agcgctccg gaaatatac ctcgatagc 
      cccaccagc acacaagct gaccctaaa actgacatt 
      tggtcgctc ggaattgtt ttaattgaa ttaatttta 
      aacaaagtc ttgtggcag tctttgaag ttggggcaa 
      agaatccgg aaaatttta agtcttgtg cagtgcaac 
      acctcggtc tttgagcga attgcgcgc gagcacaac 
      tgttacgac aaatatgag agtgtcccc aacgatttg 
      agagaaatt attgaagcg tgtctcaat atttacccc 
      cagaaccgc ccaacatgt cacgaatta ctggccaac 
      cccgttttt cagtctttc aagtcggaa aaaacggtc 
      aataagagc aaaatttgc gagccgttt gaaattttt 
      acaattcgt gaattgtat cactggtgg cagttggcc 
      ggaggcgac gtttttcaa gaactcaaa aagcaagga 
      ttgatacgt tcaagcccc cctattctg tcgctccca 
      aatttggtg actattgaa ggcacaaca gtaggccaa 
      gagcgcaac cctgccact ctgtacgac ccccgaatt 
      gtccaaatg cccctcgac acgctctac caacgcttt 
      gcccacatt ccgttaaat tgtttctat ccattgatc 
      cacagtact tcgaaaata atcgaaagc ggggttccc 
      ccggcttac gatgcgaca ggtttgcct ctagttatt 
      agggaaaga gacccggag tatcagttt catagggtc 
      attctgttc cgcaggctt ttacacggg tacccccat 
      acgaaagac atgattttg aaggaggcc tcaaaggac 
      attccccca cttctcaga ggggagatt tgggccgct 
      ttgctcgac gttaaaggc gactacgag cgccagtat 
      ttgcgaatt gataaagaa acctcgact acgactgac 
      cgccaaatc gaagttgat attccgcgt tgccaccaa 
      tacaacgaa ctgctctcg tctgccgaa ggccacaag 
      aaactgaaa cgcattctc aaagcttgg gtttaccaa 
      aactcgaat tacgtttac tggcaaggc ttggattcc 
      ctcacagcg ccttttctc ttcttgaat ttcaacaac 
      gaagccaaa gcttttgct tgtttgtcc gctttcatc 
      cccaaatat ttacacaag tttttcctc aaagacaac 
      tcaatggtc attcaagag tatttagcc aaattctcg 
      caactgatc gcgttccac gacccgcac ctcgccaat 
      cacctccac gagattaac ttttatccg gaattgttc 
      gccattccg tggtttttg accgtgttt tcacacgtt 
      ttcccgctc tacaagatc ctgcatttg tgggacaag 
      ctgttattg ggcgactcc tcatttccg ctacatatc 
      ggactgtct gtattaaca caattgagg gaccgtctg 
      ctcaattct ggtttcaac gagtgtatt ttactattc 
      tccgatttg ccggaggtc gatatcgag aaatgcgtc 
      tcctattcg actgccacg ttccaatcg actcccaaa 
      agtataaca gcgcgggaa caccagaac gggaaatac 
      cacccgaaa aatgagcta gacataagc aatgtgagt 
      ttgcaggag ttgaaccgg gagaggtgc ccccggctc 
      agtgcttcc gacttcgtc gatttggtg cgacttgag 
      ccggaaaaa ctcctgatt attgatatc cgcaatccg 
      gtgcagttt aaccgctcc tcggtcgtc aatagcgta 
      aacatcccg ttttctagc gtaaccttc ggcgaagcc 
      agcctggaa aacgtgggc caacacagc acgacaata 
      aaaaacagc gggggcaaa ataatcgta attgtgggg 
      aacgaagag accgatttg gagctgttc cccaaattt 
      ttgctcaag tgtggcgtg agcagggtg tgcgtcttg 
      cacggcggc ttcagtctt ctgccaacg atcacgccg 
      tcgatttta atctcgcaa tcaaatctg atcaattaa
    mRNA sequence
    • >TC000615 RA
      gcgctttat ccatagaaa tttgaaaaa tttgcgcca 
      catctgaca gtcgatata acctcaatt ttcgctcag 
      cttcacaac gcaaacgcc ccaaaatcg gcgaaaatg 
      ccggtgcct cttttgaaa gactcccaa atgcagttt 
      ggggctatc acatttttt gcaaagcaa cataaaggc 
      gatacgtgc gggagtaac ggtcttcct ctaactcca 
      aactcaata attataatt ggaagggca caaattctc 
      aaaacaatc aaaaatccg tacttatgc caatattta 
      gatatagtg aggggcaaa cacgaaagg acagtggtc 
      gtgtcccag tattgtggg acccccttg tctgattat 
      atcacaaaa gagccgttc gattacgac gacgttaga 
      agattcgct tatcaaatt ttgaccgct ttgaattat 
      ttacatgaa cgcaaaatc gtgcataga aatttaagc 
      acggaaaac atcttattg cagaaaaac aaagacatt 
      aagttgttc aattacggc cttttttat atgacagga 
      tgcggacaa ttagtttca ttcccaata atgcaaata 
      ttatatagc gctccggaa atatacctc gatagcccc 
      accagcaca caagctgac cctaaaact gacatttgg 
      tcgctcgga attgtttta attgaatta attttaaac 
      aaagtcttg tggcagtct ttgaagttg gggcaaaga 
      atccggaaa attttaagt cttgtgcag tgcaacacc 
      tcggtcttt gagcgaatt gcgcgcgag cacaactgt 
      tacgacaaa tatgagagt gtccccaac gatttgaga 
      gaaattatt gaagcgtgt ctcaatatt tacccccag 
      aaccgccca acatgtcac gaattactg gccaacccc 
      gtttttcag tctttcaag tcggaaaaa acggtcaat 
      aagagcaaa atttgcgag ccgtttgaa atttttaca 
      attcgtgaa ttgtatcac tggtggcag ttggccgga 
      ggcgacgtt tttcaagaa ctcaaaaag caaggattg 
      atacgttca agcccccct attctgtcg ctcccaaat 
      ttggtgact attgaaggc acaacagta ggccaagag 
      cgcaaccct gccactctg tacgacccc cgaattgtc 
      caaatgccc ctcgacacg ctctaccaa cgctttgcc 
      cacattccg ttaaattgt ttctatcca ttgatccac 
      agtacttcg aaaataatc gaaagcggg gttcccccg 
      gcttacgat gcgacaggt ttgcctcta gttattagg 
      gaaagagac ccggagtat cagtttcat agggtcatt 
      ctgttccgc aggctttta cacgggtac ccccatacg 
      aaagacatg attttgaag gaggcctca aaggacatt 
      cccccactt ctcagaggg gagatttgg gccgctttg 
      ctcgacgtt aaaggcgac tacgagcgc cagtatttg 
      cgaattgat aaagaaacc tcgactacg actgaccgc 
      caaatcgaa gttgatatt ccgcgttgc caccaatac 
      aacgaactg ctctcgtct gccgaaggc cacaagaaa 
      ctgaaacgc attctcaaa gcttgggtt taccaaaac 
      tcgaattac gtttactgg caaggcttg gattccctc 
      acagcgcct tttctcttc ttgaatttc aacaacgaa 
      gccaaagct tttgcttgt ttgtccgct ttcatcccc 
      aaatattta cacaagttt ttcctcaaa gacaactca 
      atggtcatt caagagtat ttagccaaa ttctcgcaa 
      ctgatcgcg ttccacgac ccgcacctc gccaatcac 
      ctccacgag attaacttt tatccggaa ttgttcgcc 
      attccgtgg tttttgacc gtgttttca cacgttttc 
      ccgctctac aagatcctg catttgtgg gacaagctg 
      ttattgggc gactcctca tttccgcta catatcgga 
      ctgtctgta ttaacacaa ttgagggac cgtctgctc 
      aattctggt ttcaacgag tgtatttta ctattctcc 
      gatttgccg gaggtcgat atcgagaaa tgcgtctcc 
      tattcgact gccacgttc caatcgact cccaaaagt 
      ataacagcg cgggaacac cagaacggg aaataccac 
      ccgaaaaat gagctagac ataagcaat gtgagtttg 
      caggagttg aaccgggag aggtgcccc cggctcagt 
      gcttccgac ttcgtcgat ttggtgcga cttgagccg 
      gaaaaactc ctgattatt gatatccgc aatccggtg 
      cagtttaac cgctcctcg gtcgtcaat agcgtaaac 
      atcccgttt tctagcgta accttcggc gaagccagc 
      ctggaaaac gtgggccaa cacagcacg acaataaaa 
      aacagcggg ggcaaaata atcgtaatt gtggggaac 
      gaagagacc gatttggag ctgttcccc aaatttttg 
      ctcaagtgt ggcgtgagc agggtgtgc gtcttgcac 
      ggcggcttc agtcttctg ccaacgatc acgccgtcg 
      attttaatc tcgcaatca aatctgatc aattaactt 
      gcgtgctat ttataccga cttgtttcc atgttattt 
      atgtttata ataaatgac cgtttgtta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY