Status
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Gene information
General information
TC000626 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8885756..8888483
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000626
    iBeetle screen iB_09631

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000626 RA
      MDNFCKFRV IYSEKQREA IETEFVRLS GQYYLEHAG 
      ATLYSEKQL EEIAHQLCT NLYANPHAK NLSSKLTED 
      AIDIVRYQL LAHFKTTPD EYSVVFTSG ATASLKLVA 
      ENFKYGPDG SLVYLQDNH TSVLGMRAY APHTKCIKF 
      TETLSQCKT AKSGNSLFV FPAQSNFSG VKYPLSWIK 
      AVKKGALGP GEWYVVLDA AAFAPTEVM DLSEIKPDF 
      VAISFCKIF GYPTGLGAL LVRNESCGV LRKRYYGGG 
      TVFMAQAVR DEVVMRDVI HERFEDGTV PFLSILALK 
      AGFNTLKRL DLSFETISR HTFSLAQYV YRNLLCLHH 
      SNGKPAVVL YHNTTFENP QDQGPIVNF NILRDNGEY 
      VGYAEVSHF ANLYNIHLR TGCFCNPGA CQYFLNLSP 
      EDVKKHYEA GHVCGDQHD LVDGYPTGS VRVSFGYMS 
      RKIDADKLL EMVENCFVT KPVIKKIPH KGRNDYLIP 
      TDSDYKLEE ELDNNNSEG VLKQIILYP IKSCGGFSV 
      PQWPLTSTG LKFDRQWMI INSSGVAIT QKNNKKMCL 
      IRPIIDLET EMLFLTYPG RKSFHVPIN VSSYSQNVA 
      SLCQSKVCG DKIEGWDCG DEVSDWLSE VLECPGVRL 
      LKQCDENEK IFTRKSTKN DEIQLSLVN KAQFLLINE 
      ASVEWLRGQ IREEEFFEE LGTTIQRFR ANFVVRFNK 
      EFTENEFGE FIFDDIAFE AGGVCTRCQ MICIDQTTG 
      TTSKEPLLT LSKCFKGKI TFGVYLNRT KYDFERCLY 
      VGSKVNGTK LK
    CDS sequence
    • >TC000626 RA
      atggataat ttttgcaag ttccgtgta atatattcc 
      gaaaaacag cgggaagcc attgagacg gaatttgtg 
      cgtctttca ggtcaatac tatttagag catgccgga 
      gctacttta tattcagag aaacaactc gaggaaatt 
      gcccaccaa ttatgtacc aacttgtac gcaaaccca 
      cacgcgaaa aacctctcc agtaaacta acagaagac 
      gctattgat atcgtgcgt tatcagctt ttagcccac 
      tttaaaacc acccctgac gaatactcc gtcgtcttc 
      acatcaggc gccaccgct tctctgaaa cttgttgcg 
      gaaaatttc aaatacgga cctgacggt agcctcgtc 
      tacctccaa gacaaccac acgtccgtc ctcggcatg 
      cgcgcctac gccccccac accaagtgc attaaattc 
      acagagact ttatcacaa tgcaagacc gcaaaatcg 
      gggaattcc ctgtttgtc tttcccgcg cagagcaac 
      ttctcgggg gtgaagtac ccgctttcg tggatcaag 
      gctgttaag aagggggcc ctggggccg ggggagtgg 
      tacgtggtg ttggacgcc gccgctttt gcccccaca 
      gaggttatg gatttgtcc gaaattaag ccggacttt 
      gtggcgatc tcgttttgc aaaattttc gggtatccg 
      accgggctg ggggcgctt ttggtgcga aatgagagt 
      tgtggggtc ttgaggaag aggtattac ggcggaggg 
      acggttttt atggcgcag gcggtaagg gatgaggtt 
      gtgatgagg gatgtgata catgaaagg tttgaggac 
      ggtactgtt ccatttctc tcaattttg gctcttaag 
      gcgggcttt aacaccctc aagcgactg gatttgtcc 
      ttcgaaacc atctcaaga cacactttt tcgttggca 
      cagtacgtc tacaggaac ttgctttgt ttgcaccac 
      tcaaacggc aaacctgct gttgttttg taccacaac 
      accacgttt gaaaacccg caagatcaa ggccccatt 
      gttaatttt aatattttg cgcgataac ggcgaatat 
      gtcggttat gccgaagtg tcgcatttt gcaaatttg 
      tataacatt catttgaga actgggtgt ttttgtaat 
      cccggtgct tgtcagtac tttctcaat ttgagtcct 
      gaagatgtg aaaaaacac tatgaggca ggtcacgtg 
      tgtggggac cagcacgat ttggtggat gggtacccc 
      acaggttcc gtccgcgtt tcatttggg tacatgtcc 
      cggaaaatt gacgctgat aaattattg gaaatggtc 
      gaaaattgc ttcgttacc aagccagtg attaaaaaa 
      atcccgcac aagggacgt aacgattat ttaatccca 
      accgacagt gactataaa ctggaggaa gaactggac 
      aataacaat tcggagggt gttttgaaa cagatcatt 
      ttatacccg attaaatcc tgtgggggt ttttcagtg 
      ccacaatgg ccacttacc tccacaggt ttgaaattt 
      gaccggcaa tggatgata atcaactca tccggcgtt 
      gcaattacg caaaaaaac aacaaaaaa atgtgtctc 
      attcgtccg atcatcgat ctggaaacc gaaatgttg 
      ttcctaacg tatccaggt cgtaagagc ttccacgtt 
      ccgataaat gtctcttcg tattcgcaa aatgtcgcg 
      tctctctgt cagagtaaa gtttgcggg gataaaatc 
      gaagggtgg gattgcggg gatgaagtt tctgattgg 
      ttaagtgaa gttttggaa tgtccggga gtccgtttg 
      ttgaaacag tgcgacgaa aacgagaaa atcttcacg 
      aggaagagc acgaaaaat gacgaaatt cagctgtcg 
      ctggtcaat aaagctcaa tttttgtta ataaacgag 
      gccagtgta gagtggttg agggggcaa atccgtgag 
      gaggagttt tttgaggag ttggggacg acgattcag 
      agattccga gcgaatttt gttgtcagg tttaataag 
      gaatttacg gaaaatgag ttcggcgag tttattttt 
      gacgacatt gcgtttgag gccggggga gtgtgtaca 
      aggtgccaa atgatttgc attgatcaa actaccgga 
      accacttca aaagagcct ttactaacg ctgtctaaa 
      tgtttcaaa gggaaaatc acgtttggc gtttattta 
      aatcggacc aaatatgat ttcgagcga tgtctttac 
      gtcggatcc aaagtcaat ggaacaaaa ctgaaataa
    mRNA sequence
    • >TC000626 RA
      tcgatcttt taatatttt attattaaa caatcctca 
      agtgcctta aacaaaaga tggataatt tttgcaagt 
      tccgtgtaa tatattccg aaaaacagc gggaagcca 
      ttgagacgg aatttgtgc gtctttcag gtcaatact 
      atttagagc atgccggag ctactttat attcagaga 
      aacaactcg aggaaattg cccaccaat tatgtacca 
      acttgtacg caaacccac acgcgaaaa acctctcca 
      gtaaactaa cagaagacg ctattgata tcgtgcgtt 
      atcagcttt tagcccact ttaaaacca cccctgacg 
      aatactccg tcgtcttca catcaggcg ccaccgctt 
      ctctgaaac ttgttgcgg aaaatttca aatacggac 
      ctgacggta gcctcgtct acctccaag acaaccaca 
      cgtccgtcc tcggcatgc gcgcctacg ccccccaca 
      ccaagtgca ttaaattca cagagactt tatcacaat 
      gcaagaccg caaaatcgg ggaattccc tgtttgtct 
      ttcccgcgc agagcaact tctcggggg tgaagtacc 
      cgctttcgt ggatcaagg ctgttaaga agggggccc 
      tggggccgg gggagtggt acgtggtgt tggacgccg 
      ccgcttttg cccccacag aggttatgg atttgtccg 
      aaattaagc cggactttg tggcgatct cgttttgca 
      aaattttcg ggtatccga ccgggctgg gggcgcttt 
      tggtgcgaa atgagagtt gtggggtct tgaggaaga 
      ggtattacg gcggaggga cggttttta tggcgcagg 
      cggtaaggg atgaggttg tgatgaggg atgtgatac 
      atgaaaggt ttgaggacg gtactgttc catttctct 
      caattttgg ctcttaagg cgggcttta acaccctca 
      agcgactgg atttgtcct tcgaaacca tctcaagac 
      acacttttt cgttggcac agtacgtct acaggaact 
      tgctttgtt tgcaccact caaacggca aacctgctg 
      ttgttttgt accacaaca ccacgtttg aaaacccgc 
      aagatcaag gccccattg ttaatttta atattttgc 
      gcgataacg gcgaatatg tcggttatg ccgaagtgt 
      cgcattttg caaatttgt ataacattc atttgagaa 
      ctgggtgtt tttgtaatc ccggtgctt gtcagtact 
      ttctcaatt tgagtcctg aagatgtga aaaaacact 
      atgaggcag gtcacgtgt gtggggacc agcacgatt 
      tggtggatg ggtacccca caggttccg tccgcgttt 
      catttgggt acatgtccc ggaaaattg acgctgata 
      aattattgg aaatggtcg aaaattgct tcgttacca 
      agccagtga ttaaaaaaa tcccgcaca agggacgta 
      acgattatt taatcccaa ccgacagtg actataaac 
      tggaggaag aactggaca ataacaatt cggagggtg 
      ttttgaaac agatcattt tatacccga ttaaatcct 
      gtgggggtt tttcagtgc cacaatggc cacttacct 
      ccacaggtt tgaaatttg accggcaat ggatgataa 
      tcaactcat ccggcgttg caattacgc aaaaaaaca 
      acaaaaaaa tgtgtctca ttcgtccga tcatcgatc 
      tggaaaccg aaatgttgt tcctaacgt atccaggtc 
      gtaagagct tccacgttc cgataaatg tctcttcgt 
      attcgcaaa atgtcgcgt ctctctgtc agagtaaag 
      tttgcgggg ataaaatcg aagggtggg attgcgggg 
      atgaagttt ctgattggt taagtgaag ttttggaat 
      gtccgggag tccgtttgt tgaaacagt gcgacgaaa 
      acgagaaaa tcttcacga ggaagagca cgaaaaatg 
      acgaaattc agctgtcgc tggtcaata aagctcaat 
      ttttgttaa taaacgagg ccagtgtag agtggttga 
      gggggcaaa tccgtgagg aggagtttt ttgaggagt 
      tggggacga cgattcaga gattccgag cgaattttg 
      ttgtcaggt ttaataagg aatttacgg aaaatgagt 
      tcggcgagt ttatttttg acgacattg cgtttgagg 
      ccgggggag tgtgtacaa ggtgccaaa tgatttgca 
      ttgatcaaa ctaccggaa ccacttcaa aagagcctt 
      tactaacgc tgtctaaat gtttcaaag ggaaaatca 
      cgtttggcg tttatttaa atcggacca aatatgatt 
      tcgagcgat gtctttacg tcggatcca aagtcaatg 
      gaacaaaac tgaaataaa tttttatat ttttattgc 
      ctgtttttt atttgaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY