Status
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Gene information
General information
TC000957 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8869925..8884180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000957
    iBeetle screen iB_09630

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000957 RA
      MNIMRKLRG GGGGTPSAS SSDISDGGP GSPHTQLGL 
      MHLKKLFSE YTHPSHPLT DQERDDKLY NMLPLFCKV 
      FGSSPTCDM NEKFWDILA FCHQVSLLM VSEIRKRAS 
      NQSTEAASC AIAKFLEIE NCEESSNGW MLLSTLNLL 
      AAGDATLVQ VMSEVSVPS TLVKCLYLF FDLPEMSEQ 
      EANSKDSSS DFTPRERRI LLQKIFVQL LVRLCSHRI 
      PAEELARKD DLTLLFSAI TSWCPSHNV MWRKSAAEV 
      LMTLSRHGL TPPVVGYIH SKGCVALCI ENMKRVPEL 
      APLELVEMF VTVFCFLKD SSEVSSTLL DDFRQAQGY 
      LFLSDFLLK LESEKSSEA QESIRNLVL MVTSLCMCG 
      YIELKPSQA STGSLFQMQ GFTLPQPSG RGTSVRNIQ 
      AFHVLQSTF LKSNSPLLC CTILDAISS IYHSDNANY 
      FILENQNTL SQFTEKIHY KSEEIQSKL FEMIEFLVF 
      QLNFVPCKE LISMSIFLK THSLNHVDC SILCVQTLL 
      NILKHNVIF KDVYREVGM LEVFVTCLN RYLTFLEEK 
      KSKPEFKIP PGQEKLGTL IVEALTHLL AGNPSNATV 
      LRECGGAKC VQELVQYSE CRYSALGII KELVLTTGG 
      DEDMAQLLT TLHSAPRTA LQLKIDILE ALKLCLKES 
      HRTRTVFRK VNGFVYVTS VLVALEGKL RQKETNPEV 
      LTLLQLVFH TISTAMRFE PANAKFFYH EICKTSLCD 
      TLRLLGCFT DKVDVIKEC DSEAPSNDL QDVYHKLFV 
      GSVTNPELS DLVAPSLSY ATITYRLLY DLALDLFDK 
      PNVILMKSP SLSRQASIE PPPNSGKRS INSLNLTPP 
      IPDPIIVHP GVVVAMLQL LPCINSSDP GLTLQLYIS 
      EVIKSLVRS ERNQQVMCD KEFVGYLLS IGSTPIQNE 
      THPLHGPLQ YMLERLAAQ ALEPTDLRN FLRLGNPLC 
      CLALESKEQ GGGPVPLTR IKTLVSMTT PKDFRAQSS 
      YTLPPFVEF DMSAEGFGC LYLPSIAPQ STAAPSVVS 
      TLDSSVLGG IGSGDRLFP PQTGLSYST WICVDKFSD 
      PRTDPHCVR LLTLVRNFN AAREDHLVC LSIVLSARD 
      KAIIVSTQE THIPHHVGD WEPEGSGEH GARVWCPDI 
      LQEGQWHHI VVVLNRAVL KNSSFALYL DGQQLHAQK 
      MHYISQNPG GGSANLTVA SSVYGFIGT PPAWRRYSR 
      LCWKQGPCH LFEEVLTPV VVSQMFHLG PHYMGSFLA 
      PNLNTSDTM QLVSEEKVV FGLNAKAVS QLTLNKIRK 
      VYSRTDNKS IAKQLGMSS HESATPIRI LHNSAGHLA 
      GSARSLGGV VIGYLGVRV FCPRPVATT IDTVGGCSV 
      LLGLIAMAQ DVESLYAGV KALVCVVKS NKVAQAEMD 
      RKRGYQTLA MLLRRKRVL LNSHILHLA FSLVGTVDS 
      GRESSAIPN RTAFQDLLC DLEVWHEAP GELLRSHLE 
      HLYELAAES NEKRNNTWI MRDLQLVEK LLHITPEVT 
      HNATRQVLF SLLSVLLGG QPRQPDLLL FGQFIAATL 
      PQSSNVNEK PLVLRESDS EKEGEGENI LLRNRCLGL 
      VHSMLFAQR NIVGEEIAR TLGFDWILL FMQPNLHAT 
      TVVWGLRIL VVMCSNPGL MNRFRDGSS NGGWLRYTE 
      QVTHNKMAV VLGCHMNSN QGNDTKSEA LHIPGFQYL 
      GWLLPSHLD VPEIYFLLT ALIMGVPVK LLPAETKFD 
      LDAIWTFLW GSSISNQPA TSVAPRICL CPEAVVVLL 
      TMARSMLHT SDSTLPEWL QNHPISIIQ VLFQLYHNM 
      PDFMPIFMS SEVLGALAG VLFPPPVVS SMDSGDSSG 
      ASTPAGDET EVIVVPRTP DEDPLTTHP VRQFIIDFI 
      RVIVVDSLS LSTTGKASP VIDLVLDAF PVNSTASQQ 
      ICYQTEILV TLMDHLLAA DMLVGDQAA IPVVPLANA 
      HIQHVTPNV FYLTARIVD KLWQGSLTK DPHEVFDFI 
      VKLIGQAKR RTGNLSLEC LYHCLNRSI LFLLSRPTD 
      TIADQMSVL EALHKLTTN RLIVFGAGN HELDFIGCL 
      TYCLLQLTA DMKIMLESN MRTTWHVNP SSDLESRDE 
      QLNAHQGRN LMAGAALRV WEELYVCKK PAIEEVFKV 
      TLAFPNPGC KAPDLTIVR EQVFDAASK LWLNYVDAE 
      RKATYRVPW ELHNQIQSK IHKVTGGLT RLASRTKVR 
      KDDTMRIKI RMSKKQALQ WMINHVNLL REFVEMRRQ 
      QHQNTTQHT QRYVHQEWL QTENELTRE RGLWGPPEP 
      CYLDKWMLD MTEGPHRMR KKTMRNDLF YLHYPYRPE 
      HEKGLLKYK VATSHHSKI YYQAVQNKR TGVTGLTEP 
      ERADSIVDE PSPLPLTPL PPLARLKSD PDDQQEEND 
      VEEEPSAPP DNQTLMRLL EENEKISHM FRCARIQGL 
      DTTEGLLLF GQEHCYVVD GFTLLKNRE IRDIEGVPN 
      NAHEYEPIL PNPGSPRRS RAMRQCSKF SYEDIREVH 
      KRRYLLQPM ALEVFSGDG RNYLLAFPR KVRNKVYQR 
      FMAMATGIA DSAQQSVAG QRRTANVEQ ATSLLSNLI 
      GETSVTQRW VRGEISNFQ YLMYLNTLA GRSYNDLMQ 
      YPVFPWILA DYDSEELDL TDPTTFRDF TKPMGAQSP 
      ERLEQFRKR FKEWDDPHG ETPPYHYGT HYSSAMIVC 
      SYLVRSEPF TQHFLRLQG GHFDLADRM FHSVKEAWL 
      SASKHNMAD VKELIPEFF YLPEFLENL NQFDLGAKQ 
      SGVALGDVV LPPWAKQDP REFIRVHRM ALECDYVSQ 
      NLHHWIDLI FGYKQQGQP AIDSVNVFH HLFYEGNVD 
      IYNIDDPLK KNATIGFIN NFGQIPKQL FKKPHPCKK 
      MGGGNNRTS VIDTGSLVQ AFTLPQPEK LFFHHLDNL 
      RPSLQPIKE VKSPVGQIL HVDKTVLAV EQNKVLIPT 
      LYNKYVAWG FADHSLRIG NYDSDKAVF VSESVVQNN 
      GEILACVCP SPKLIVTAG TSSVVTIWE FESRKKSLN 
      IKHNLYAHT DAVTCLAAS PAYNVIVSG SRDCTAIIW 
      DLSRGIFVR QLRGHAGPI AAVAVNDLT GDIATCAAT 
      WLHVWSING DELASVNTC VGRADRMQQ ILCVAFSQT 
      HEWDSLNVV MTGSTDGVT RMWSIEYVQ VPDETNTNM 
      EPKHQSPVR PTPIHHRLV KQINVSNDS SLSLMKSGS 
      ESSLSEDNN TRRPSIDRQ ASSKEWRSE QQNEKETCS 
      DTEEPSPPI PVRRRRSRV HGGFRKSEG GNSADNASL 
      DVDDGTSMR TSKSDTSLT DSFVMVPES KKRTVNPMN 
      VLRPGFKWQ RQLVFRSKL TMHTAYDRK DNAEPASIT 
      ALAVSKDHR SVYVGDTRG RVFSWSVCD VPGRAADHW 
      LKDEGADFC ASCNIRFTI YERKHHCRN CGQVFCSKC 
      SRFESEISR LRILKPVRV CQTCYATLK SEK
    CDS sequence
    • >TC000957 RA
      atgaatatc atgcgaaag ttgcgaggt ggaggcggt 
      ggcacccct tccgcctcc tcatcagac atatcagac 
      ggcggcccc ggttcccct cacacccaa ttgggtctg 
      atgcatctc aaaaagctt ttctcggag tacacgcac 
      ccgtcacat ccattaaca gaccaagag cgggatgat 
      aaactttac aacatgctc ccgcttttt tgcaaggtc 
      ttcggatcg agtcccacg tgcgacatg aatgagaaa 
      ttctgggac atactcgcg ttctgccac caagtgtcg 
      ctccttatg gtgtcggaa ataaggaag cgggcctcg 
      aatcagagc acggaagcg gcgagctgt gccattgcg 
      aaatttcta gaaattgaa aattgcgaa gagtcgagc 
      aatgggtgg atgcttcta agcactttg aatctttta 
      gcggcggga gatgcgact ttagtccag gtcatgtcc 
      gaggtttcg gtcccgtca acgttagtc aaatgcttg 
      tacttgttc ttcgatttg ccggaaatg tccgaacag 
      gaagccaac agtaaagac tcatctagt gatttcacg 
      ccacgcgaa aggagaatc ctgctccag aaaattttt 
      gtacaatta ttggtccgt ctgtgtagt catcgaatt 
      cccgccgaa gaattggct cggaaggac gacctgacg 
      ttattattt tctgctatc acttcatgg tgtccctca 
      cacaacgtc atgtggagg aaaagtgca gccgaagtc 
      ctaatgacc ctctcccgg catgggctt acgccccct 
      gttgtgggc tacatccac tcaaaaggc tgcgtcgct 
      ttgtgtata gaaaacatg aaaagagta cctgaatta 
      gcaccacta gaattagtc gaaatgttc gtaaccgtt 
      ttttgtttt ttgaaagat tcgagcgaa gtctcctcg 
      acgctttta gacgacttt aggcaagct caaggttat 
      ttgttcttg tcggatttt ttactcaaa ttggaaagt 
      gaaaagtcg agtgaggca caagaatcg atccgaaat 
      ctagttctt atggtcact tcgttgtgc atgtgtggg 
      tatattgag ctgaagccg tcacaggcc tccactggt 
      tcgttattt caaatgcaa ggttttacg ctaccgcag 
      ccctcgggg cgcggcacc agtgtgcgc aatattcaa 
      gcatttcac gtattacag agcacattc ctgaaatct 
      aactctccc ctcctgtgc tgcacaatc ctggacgca 
      atctcaagc atttaccac tcggacaac gccaattac 
      ttcatcctc gaaaaccaa aatactctg agccagttc 
      accgaaaaa atccactac aagtctgaa gaaatccaa 
      agcaaactg ttcgaaatg atcgagttt ctcgtattc 
      caattaaac ttcgtacct tgcaaagag ctaatctcc 
      atgtcaatt ttcctcaaa acccatagt ttaaaccac 
      gtagactgt agtatatta tgtgtacag accctgttg 
      aacatattg aaacacaac gttatattt aaagatgtt 
      tatcgcgaa gttggcatg ttggaagtt ttcgttact 
      tgtttaaac cgttattta actttcctc gaagagaaa 
      aaatccaag cccgagttt aaaataccc cccggtcag 
      gaaaaactg gggaccctt atcgtggaa gccctcact 
      cacctcctg gctggaaac ccgtcaaac gccacggtt 
      ttacgcgaa tgtggcgga gccaaatgc gtgcaagaa 
      ctagtccag tattccgag tgtcgatac tcggcgtta 
      ggtatcatc aaagaattg gttctcacc acgggaggt 
      gacgaagac atggcccaa ctcctcaca actctgcat 
      tccgctcca agaaccgca ctccaactc aaaatcgat 
      attctggaa gctctgaag ttgtgtctg aaggagtcg 
      caccgaact cggactgtt tttcgtaaa gtcaacggg 
      ttcgtttac gtgacgagt gttttggtg gcgttggag 
      ggaaagttg cggcagaag gagacaaat ccggaggtt 
      ctaacactc ctacagttg gtttttcat acgatttcg 
      accgccatg agattcgaa ccggccaat gccaagttc 
      ttctaccac gaaatttgc aaaactagt ctttgtgat 
      actcttaga ttattaggc tgtttcacc gacaaagtc 
      gatgttatc aaagaatgt gattcggaa gcaccaagc 
      aatgacttg caagatgtt tatcacaag ttgtttgtc 
      ggatctgtt acgaatcct gagttgtcg gacttagtg 
      gccccgtct ctctcatac gccaccatc acctaccgc 
      ctcctctac gacttggct ctcgacctt ttcgacaaa 
      cctaacgtc atcctcatg aaatccccg tccttgtcc 
      cggcaagct tccatcgaa ccgccccct aactccggt 
      aaacgctcc atcaactcc ctcaacctc actccccca 
      atccccgac ccgatcata gtccaccca ggggtcgta 
      gttgcaatg ttacaactc ctcccttgt atcaactca 
      tcagacccc ggcctcacc ctccagtta tacatttcg 
      gaagtgatc aaatccctc gtgcggagt gaacgtaac 
      caacaagtc atgtgtgat aaagaattc gtcggttat 
      ttgctctcg atcgggtca acccccatt caaaacgag 
      acgcacccc ctccatggg cccctccaa tacatgcta 
      gaacgtctg gcagcccag gctttggaa cccaccgat 
      ttacgcaat ttcctccgt ttggggaac cccctgtgt 
      tgcctggcg ttggagagt aaggagcag gggggaggt 
      ccggtgcct ttgacacgg atcaaaacc ttggtctcg 
      atgactact ccgaaggat ttccgggcg cagtcgtcg 
      tacactctt ccgcctttt gtggagttt gacatgtcg 
      gcggagggg ttcggctgt ttgtacctc ccgagtatc 
      gctccacag tccactgca gccccaagt gttgtctcc 
      actttggat agtagtgtc ttggggggg attgggtca 
      ggagatcgc ttgtttcca ccacagaca ggcttgagc 
      tactccact tggatttgt gttgataaa ttctctgac 
      ccgagaact gatccacac tgcgtacgg ttgttgacc 
      cttgtcagg aacttcaac gcggcacgg gaggaccac 
      ttggtgtgc ctctctatc gtgctgtca gcgagggac 
      aaggccatt atcgtatca acccaagag actcacatt 
      ccgcaccat gtaggtgat tgggagccg gagggctct 
      ggcgagcac ggagcaagg gtctggtgc cccgacatc 
      ctccaagaa ggccaatgg caccacatt gtcgtggtc 
      ctgaaccgg gctgttctt aaaaactcg tcctttgcc 
      ctgtatctc gatggccaa caactccac gcgcaaaaa 
      atgcactac atatcgcaa aacccaggt ggagggagt 
      gccaatcta acggtggct tcgtccgtt tatggtttt 
      attggaacc cccccagct tggaggagg tactcccga 
      ctgtgttgg aagcaaggc ccttgtcac ttgttcgaa 
      gaggttcta accccagtc gtggtctcc caaatgttc 
      catttgggg cctcactac atgggctct ttcctcgcc 
      ccaaacctc aacacttcg gacacaatg cagttggtg 
      tcggaggaa aaagtcgta ttcggactc aacgctaaa 
      gccgtttcg caactcacg ttgaacaaa atccggaaa 
      gtttacagt cggactgac aacaaatca attgccaaa 
      caactcgga atgtcgagt catgaaagt gccactccg 
      attcggatt ttgcacaat tcggccgga catttagcc 
      gggtctgct aggagttta ggcggagtc gttatcggg 
      tatttaggg gttagagtt ttttgcccg cgacctgtt 
      gccacgact attgacact gtcggtggc tgttccgtg 
      ttattgggc ttgattgcg atggcgcaa gacgtggaa 
      agtttatac gccggtgtg aaagcccta gtttgcgtc 
      gttaaaagc aacaaagtg gcgcaagcc gaaatggac 
      cgcaagcgg ggctaccag accttagcc atgctgctg 
      aggcgcaaa cgggtgctc cttaactcg catatctta 
      cacctcgcc ttcagcctc gtcggcacg gtggacagc 
      ggccgggag tcctcagcc atccccaac cgaacggcg 
      tttcaggac ctcctgtgc gacttggaa gtctggcac 
      gaagccccc ggcgaactc ctccgctcc cacctggag 
      cacttgtac gaactcgcc gccgaatct aacgagaaa 
      cgcaacaac acgtggata atgcgcgac ttgcaactc 
      gtcgaaaaa ctcctccac atcacgccc gaagtcaca 
      cataacgcc acgcggcaa gtcctcttc tccctgctt 
      tcggtgctc ttagggggg caaccgcgc caacccgac 
      ttgttgctg ttcgggcaa ttcatcgca gcgacactt 
      ccccaatcg tcaaacgtt aacgaaaaa ccgctagtt 
      ttgcgtgaa agtgactcg gagaaggaa ggcgaaggc 
      gagaatatt ctcctgagg aaccgctgt ttggggctg 
      gtgcattct atgcttttt gcgcagaga aacatcgtc 
      ggggaggaa atcgcgcgc acgttgggg tttgactgg 
      atcttgctt ttcatgcaa ccgaattta cacgccacg 
      acagtggtc tgggggttg aggatttta gtggtgatg 
      tgctcaaac cccggactt atgaatcga ttccgcgat 
      ggtagtagc aatggcggg tggttgcgc tacacggaa 
      caagtcact cacaataaa atggcagta gttttagga 
      tgccacatg aacagtaac caaggtaat gacaccaaa 
      tcggaagcc ctccacatt cccgggttt caatacttg 
      ggatggctt ctcccgtcg catttggac gtcccggaa 
      atttatttc ctactaact gcccttata atgggggtg 
      ccggtcaag cttttacca gccgagaca aagttcgat 
      ttggacgca atttggacg tttctctgg ggcagctcc 
      ataagcaac caaccggcg actagtgtg gccccccgg 
      atttgcctt tgtcccgaa gctgttgtc gttttattg 
      acaatggca aggtccatg ctccacacc agcgactcg 
      actttaccc gagtggttg caaaaccac cccatttca 
      atcatccaa gttctgttc caattgtac cacaacatg 
      cccgacttt atgcccatt tttatgtcg tctgaggtg 
      ctcggggcc ttggctggg gtcctgttc cccccacct 
      gtggtctca agcatggat agtggggat agtagcggt 
      gcctctact cccgcgggt gacgagacc gaagttatt 
      gtcgtgccg agaactcca gacgaggat ccattgact 
      acgcatccg gtcagacag tttattatc gattttatt 
      cgggttatt gtggtcgat tcgttaagt ttgtccacg 
      acagggaaa gcttcaccg gttattgac ctggttttg 
      gatgcgttt ccggtcaat tcaaccgcg tctcaacaa 
      atttgctac cagacggaa attttagtc actttgatg 
      gaccatctc ctagctgcg gatatgcta gtgggggac 
      caggcggca atcccggtt gtgccactg gccaatgcc 
      cacattcaa cacgttaca cctaacgtg ttctatctg 
      acggcgcgg attgttgat aaactgtgg caagggtcc 
      ttaactaag gacccacat gaagttttc gatttcata 
      gtcaagtta atcgggcaa gcaaaacgc cgcaccggt 
      aatctctca cttgagtgt ctctaccat tgtctcaat 
      cgcagtatt ttgttcctc ctgtcccgc cctactgac 
      accatcgcc gatcaaatg tcggtgttg gaagcctta 
      cacaaactg acaaccaat cgtttgatt gtgttcgga 
      gccggcaat cacgaatta gatttcatt gggtgcctt 
      acttactgc cttctccag ttaacagcc gatatgaaa 
      atcatgttg gagtcaaac atgcgaaca acctggcat 
      gttaaccca agcagtgat cttgaatca cgcgatgag 
      caattaaac gcccatcaa gggcgcaat ttaatggca 
      ggggcggct ttacgcgtg tgggaagaa ctctacgtt 
      tgcaaaaaa ccagcaatt gaggaggtt tttaaagtc 
      acactggcg ttcccgaac ccgggttgc aaagcaccc 
      gacttgaca atagtccgg gagcaagtg ttcgacgct 
      gcgtccaaa ctatggctc aactatgtc gacgccgaa 
      cgtaaagca acctatcga gtcccttgg gagttacac 
      aaccaaatc caatcgaaa atccataaa gttacaggc 
      ggtttaacc cgcttagcc agtcggact aaagtccga 
      aaggacgac acaatgcga atcaaaatc aggatgagc 
      aaaaaacaa gccttacaa tggatgata aaccacgtg 
      aacctcctc cgggaattc gtagaaatg cgccgccaa 
      caacaccaa aacaccacc caacacacc cagagatac 
      gtccaccaa gagtggctc caaaccgaa aacgagctg 
      acacgggaa cggggactt tggggcccc cctgagccc 
      tgctacctc gacaaatgg atgctggac atgactgaa 
      gggccccac aggatgcgc aagaagaca atgaggaac 
      gatctcttc tacctgcat tacccctac cggcccgaa 
      cacgagaaa ggcttactc aagtataaa gtcgcaacg 
      agccaccac agtaaaatc tactaccag gcggttcaa 
      aacaaaaga accggggtt acgggttta accgaacca 
      gaacgggcc gatagtatc gtggatgaa ccgtcaccg 
      ctgccactg accccgctt ccgcccctg gccaggctc 
      aagtcggac ccggacgac caacaggag gagaatgac 
      gtggaggag gaaccctca gcaccgccg gataaccaa 
      accttgatg aggcttcta gaagagaac gagaagatt 
      tcgcacatg tttcgctgc gcccgcatt caaggcttg 
      gacacaacc gaagggttg ttgctattc ggacaagaa 
      cattgctac gtcgttgat ggtttcact ctgttgaag 
      aatcgcgaa attcgggat attgagggt gtgccgaat 
      aacgcgcac gagtacgaa cccattttg cccaaccct 
      gggtcgcct agaaggtcg cgcgctatg cgccagtgc 
      tccaaattt tcatacgaa gacatcaga gaggtgcac 
      aagcggcgg tacctcctg cagccgatg gccctggag 
      gtgttttcc ggggacggc cggaactac ctcctggct 
      ttcccccgg aaagtccgg aacaaggtg tatcagcgt 
      ttcatggcc atggccact gggatcgct gatagtgcc 
      caacagtcg gtcgcgggt cagcgccgg acggccaat 
      gtcgaacaa gcaacgagt cttttaagc aatttgatt 
      ggcgagacg agtgtgaca caacgctgg gtcagaggc 
      gaaatctca aatttccag tacttgatg tacttgaat 
      actttagcc ggccgttcg tataacgat ttgatgcag 
      tacccggtg tttccctgg atcttagcc gattatgac 
      tcggaagag ctcgactta accgacccg acaactttc 
      cgtgacttc accaaaccg atgggagcc caaagcccc 
      gaacgtttg gagcaattc cggaaacgt ttcaaagaa 
      tgggatgac ccacacggg gaaacccct ccgtaccat 
      tatgggacg cactattct agtgccatg atcgtgtgc 
      tcgtattta gtccgaagt gagcctttc acgcaacat 
      ttcctgagg ttacaaggc ggacacttc gatttagcc 
      gaccgtatg tttcattcg gtcaaagag gcttggtta 
      tcggcctcc aaacacaat atggccgat gtgaaggag 
      ctgattcct gaatttttc tacctacct gagtttttg 
      gagaacttg aatcaattt gatttgggg gcgaaacaa 
      agcggggtt gctttgggt gacgtggtg ttaccacct 
      tgggccaag caggaccca cgggagttc ataagggtt 
      cacagaatg gccctggag tgtgactac gtgtcacag 
      aatctccac cattggatt gacctcatt ttcggttac 
      aaacagcaa ggacagccg gctattgac tcggttaat 
      gtcttccat catttgttc tacgaggga aacgtcgat 
      atttacaat attgacgat ccattgaaa aagaatgca 
      actataggt tttattaat aatttcggg caaattcct 
      aaacaattg ttcaagaaa ccacaccct tgtaaaaaa 
      atgggcgga ggtaataac cggacgtcg gttattgat 
      acggggtcc ctagtccaa gcgttcact ttaccacaa 
      cctgagaaa ctctttttc caccactta gacaacctt 
      agaccctcc ttacagcca attaaagaa gtgaaaagt 
      cccgtcggc caaatcctc cacgtggat aaaacagtc 
      ctcgccgtc gaacaaaac aaagtatta atcccaacg 
      ttgtacaac aaatacgtg gcttggggc ttcgctgac 
      cactcccta agaatcggg aattacgac agcgataaa 
      gccgtcttc gtgtccgaa agcgtggtc caaaacaac 
      ggcgaaatc ctggcctgt gtttgtccc tctcccaaa 
      ctaatcgtc acagctggg accagttca gtggtgaca 
      atctgggaa ttcgaatcc cggaaaaaa tccctcaac 
      atcaaacac aatctctat gcacacacg gacgcagtg 
      acgtgtctc gctgcaagt cccgcatac aacgtcata 
      gtttcgggg tcccgggat tgcaccgca atcatttgg 
      gacttatcc cgagggatt ttcgtgcga cagttacgg 
      ggtcacgct ggacccatc gccgcagtc gccgtgaac 
      gacctcaca ggggacatt gccacgtgt gcggccacg 
      tggcttcac gtctggagc ataaacggg gacgagctg 
      gcgagtgtc aacacgtgt gtcgggagg gctgacaga 
      atgcagcaa attctgtgt gtggcgttc tcgcaaacg 
      cacgaatgg gactcgttg aatgtcgtg atgacgggg 
      tccacagat ggggtcaca aggatgtgg tcgattgaa 
      tacgtgcaa gtgcccgac gagaccaac accaacatg 
      gaaccaaag caccagagt ccggtccgg ccgactccg 
      atccaccac cgtctagtc aagcagatc aatgtttca 
      aacgacagc tctctgagt ttgatgaaa tcgggctcc 
      gagagcagc ttatccgaa gacaataat acgaggcga 
      ccttcgatc gatcgtcaa gcgagctcc aaggagtgg 
      agaagtgag caacaaaac gaaaaggaa acgtgcagt 
      gacactgag gagccttca cctccgata cctgtgcgg 
      aggcgacgg tcacgagtg catggaggg ttcaggaag 
      agtgaaggt ggtaacagc gccgataat gctagtctg 
      gacgtcgac gacggcacc agcatgcgg accagcaag 
      tccgacact agcctgacc gattcgttc gttatggtg 
      ccagagagt aagaaacga actgtgaat cctatgaac 
      gtcctcagg ccagggttc aagtggcag cgccagctg 
      gtcttcagg agcaaactg acaatgcac accgcctac 
      gaccgcaag gacaacgcg gagcccgcc tctatcaca 
      gccctggcg gtctcaaaa gaccaccgc agcgtctac 
      gtgggagac acccgcggc agggttttc tcttggagc 
      gtctgcgac gttccgggt cgggcggcc gaccactgg 
      ctcaaggac gaaggagcc gacttttgc gcttcgtgc 
      aatatcagg tttacaatt tacgaaagg aaacaccat 
      tgtcgcaat tgcggacaa gtgttttgc tcgaaatgc 
      agccgattc gagagcgaa atctcacgg ttgcgtatt 
      ctgaaaccg gtgagggtt tgtcagacg tgttacgcc 
      actctcaag tcagaaaaa tag
    mRNA sequence
    • >TC000957 RA
      tcgtccgaa atagagcca tcatccata gccgaagcg 
      gtgttgcag ttgtgtttg tggataaat gcggtgtca 
      gctgtcatc gaacttgtg cagacccaa tgggcgtat 
      ttagtgcaa aaatgaaaa cacgcatcg tttcgaatc 
      cgatcaggt gcttttagg ccctcacaa tgaatatca 
      tgcgaaagt tgcgaggtg gaggcggtg gcacccctt 
      ccgcctcct catcagaca tatcagacg gcggccccg 
      gttcccctc acacccaat tgggtctga tgcatctca 
      aaaagcttt tctcggagt acacgcacc cgtcacatc 
      cattaacag accaagagc gggatgata aactttaca 
      acatgctcc cgctttttt gcaaggtct tcggatcga 
      gtcccacgt gcgacatga atgagaaat tctgggaca 
      tactcgcgt tctgccacc aagtgtcgc tccttatgg 
      tgtcggaaa taaggaagc gggcctcga atcagagca 
      cggaagcgg cgagctgtg ccattgcga aatttctag 
      aaattgaaa attgcgaag agtcgagca atgggtgga 
      tgcttctaa gcactttga atcttttag cggcgggag 
      atgcgactt tagtccagg tcatgtccg aggtttcgg 
      tcccgtcaa cgttagtca aatgcttgt acttgttct 
      tcgatttgc cggaaatgt ccgaacagg aagccaaca 
      gtaaagact catctagtg atttcacgc cacgcgaaa 
      ggagaatcc tgctccaga aaatttttg tacaattat 
      tggtccgtc tgtgtagtc atcgaattc ccgccgaag 
      aattggctc ggaaggacg acctgacgt tattatttt 
      ctgctatca cttcatggt gtccctcac acaacgtca 
      tgtggagga aaagtgcag ccgaagtcc taatgaccc 
      tctcccggc atgggctta cgccccctg ttgtgggct 
      acatccact caaaaggct gcgtcgctt tgtgtatag 
      aaaacatga aaagagtac ctgaattag caccactag 
      aattagtcg aaatgttcg taaccgttt tttgttttt 
      tgaaagatt cgagcgaag tctcctcga cgcttttag 
      acgacttta ggcaagctc aaggttatt tgttcttgt 
      cggattttt tactcaaat tggaaagtg aaaagtcga 
      gtgaggcac aagaatcga tccgaaatc tagttctta 
      tggtcactt cgttgtgca tgtgtgggt atattgagc 
      tgaagccgt cacaggcct ccactggtt cgttatttc 
      aaatgcaag gttttacgc taccgcagc cctcggggc 
      gcggcacca gtgtgcgca atattcaag catttcacg 
      tattacaga gcacattcc tgaaatcta actctcccc 
      tcctgtgct gcacaatcc tggacgcaa tctcaagca 
      tttaccact cggacaacg ccaattact tcatcctcg 
      aaaaccaaa atactctga gccagttca ccgaaaaaa 
      tccactaca agtctgaag aaatccaaa gcaaactgt 
      tcgaaatga tcgagtttc tcgtattcc aattaaact 
      tcgtacctt gcaaagagc taatctcca tgtcaattt 
      tcctcaaaa cccatagtt taaaccacg tagactgta 
      gtatattat gtgtacaga ccctgttga acatattga 
      aacacaacg ttatattta aagatgttt atcgcgaag 
      ttggcatgt tggaagttt tcgttactt gtttaaacc 
      gttatttaa ctttcctcg aagagaaaa aatccaagc 
      ccgagttta aaatacccc ccggtcagg aaaaactgg 
      ggaccctta tcgtggaag ccctcactc acctcctgg 
      ctggaaacc cgtcaaacg ccacggttt tacgcgaat 
      gtggcggag ccaaatgcg tgcaagaac tagtccagt 
      attccgagt gtcgatact cggcgttag gtatcatca 
      aagaattgg ttctcacca cgggaggtg acgaagaca 
      tggcccaac tcctcacaa ctctgcatt ccgctccaa 
      gaaccgcac tccaactca aaatcgata ttctggaag 
      ctctgaagt tgtgtctga aggagtcgc accgaactc 
      ggactgttt ttcgtaaag tcaacgggt tcgtttacg 
      tgacgagtg ttttggtgg cgttggagg gaaagttgc 
      ggcagaagg agacaaatc cggaggttc taacactcc 
      tacagttgg tttttcata cgatttcga ccgccatga 
      gattcgaac cggccaatg ccaagttct tctaccacg 
      aaatttgca aaactagtc tttgtgata ctcttagat 
      tattaggct gtttcaccg acaaagtcg atgttatca 
      aagaatgtg attcggaag caccaagca atgacttgc 
      aagatgttt atcacaagt tgtttgtcg gatctgtta 
      cgaatcctg agttgtcgg acttagtgg ccccgtctc 
      tctcatacg ccaccatca cctaccgcc tcctctacg 
      acttggctc tcgaccttt tcgacaaac ctaacgtca 
      tcctcatga aatccccgt ccttgtccc ggcaagctt 
      ccatcgaac cgcccccta actccggta aacgctcca 
      tcaactccc tcaacctca ctcccccaa tccccgacc 
      cgatcatag tccacccag gggtcgtag ttgcaatgt 
      tacaactcc tcccttgta tcaactcat cagaccccg 
      gcctcaccc tccagttat acatttcgg aagtgatca 
      aatccctcg tgcggagtg aacgtaacc aacaagtca 
      tgtgtgata aagaattcg tcggttatt tgctctcga 
      tcgggtcaa cccccattc aaaacgaga cgcaccccc 
      tccatgggc ccctccaat acatgctag aacgtctgg 
      cagcccagg ctttggaac ccaccgatt tacgcaatt 
      tcctccgtt tggggaacc ccctgtgtt gcctggcgt 
      tggagagta aggagcagg ggggaggtc cggtgcctt 
      tgacacgga tcaaaacct tggtctcga tgactactc 
      cgaaggatt tccgggcgc agtcgtcgt acactcttc 
      cgccttttg tggagtttg acatgtcgg cggaggggt 
      tcggctgtt tgtacctcc cgagtatcg ctccacagt 
      ccactgcag ccccaagtg ttgtctcca ctttggata 
      gtagtgtct tggggggga ttgggtcag gagatcgct 
      tgtttccac cacagacag gcttgagct actccactt 
      ggatttgtg ttgataaat tctctgacc cgagaactg 
      atccacact gcgtacggt tgttgaccc ttgtcagga 
      acttcaacg cggcacggg aggaccact tggtgtgcc 
      tctctatcg tgctgtcag cgagggaca aggccatta 
      tcgtatcaa cccaagaga ctcacattc cgcaccatg 
      taggtgatt gggagccgg agggctctg gcgagcacg 
      gagcaaggg tctggtgcc ccgacatcc tccaagaag 
      gccaatggc accacattg tcgtggtcc tgaaccggg 
      ctgttctta aaaactcgt cctttgccc tgtatctcg 
      atggccaac aactccacg cgcaaaaaa tgcactaca 
      tatcgcaaa acccaggtg gagggagtg ccaatctaa 
      cggtggctt cgtccgttt atggtttta ttggaaccc 
      ccccagctt ggaggaggt actcccgac tgtgttgga 
      agcaaggcc cttgtcact tgttcgaag aggttctaa 
      ccccagtcg tggtctccc aaatgttcc atttggggc 
      ctcactaca tgggctctt tcctcgccc caaacctca 
      acacttcgg acacaatgc agttggtgt cggaggaaa 
      aagtcgtat tcggactca acgctaaag ccgtttcgc 
      aactcacgt tgaacaaaa tccggaaag tttacagtc 
      ggactgaca acaaatcaa ttgccaaac aactcggaa 
      tgtcgagtc atgaaagtg ccactccga ttcggattt 
      tgcacaatt cggccggac atttagccg ggtctgcta 
      ggagtttag gcggagtcg ttatcgggt atttagggg 
      ttagagttt tttgcccgc gacctgttg ccacgacta 
      ttgacactg tcggtggct gttccgtgt tattgggct 
      tgattgcga tggcgcaag acgtggaaa gtttatacg 
      ccggtgtga aagccctag tttgcgtcg ttaaaagca 
      acaaagtgg cgcaagccg aaatggacc gcaagcggg 
      gctaccaga ccttagcca tgctgctga ggcgcaaac 
      gggtgctcc ttaactcgc atatcttac acctcgcct 
      tcagcctcg tcggcacgg tggacagcg gccgggagt 
      cctcagcca tccccaacc gaacggcgt ttcaggacc 
      tcctgtgcg acttggaag tctggcacg aagcccccg 
      gcgaactcc tccgctccc acctggagc acttgtacg 
      aactcgccg ccgaatcta acgagaaac gcaacaaca 
      cgtggataa tgcgcgact tgcaactcg tcgaaaaac 
      tcctccaca tcacgcccg aagtcacac ataacgcca 
      cgcggcaag tcctcttct ccctgcttt cggtgctct 
      taggggggc aaccgcgcc aacccgact tgttgctgt 
      tcgggcaat tcatcgcag cgacacttc cccaatcgt 
      caaacgtta acgaaaaac cgctagttt tgcgtgaaa 
      gtgactcgg agaaggaag gcgaaggcg agaatattc 
      tcctgagga accgctgtt tggggctgg tgcattcta 
      tgctttttg cgcagagaa acatcgtcg gggaggaaa 
      tcgcgcgca cgttggggt ttgactgga tcttgcttt 
      tcatgcaac cgaatttac acgccacga cagtggtct 
      gggggttga ggattttag tggtgatgt gctcaaacc 
      ccggactta tgaatcgat tccgcgatg gtagtagca 
      atggcgggt ggttgcgct acacggaac aagtcactc 
      acaataaaa tggcagtag ttttaggat gccacatga 
      acagtaacc aaggtaatg acaccaaat cggaagccc 
      tccacattc ccgggtttc aatacttgg gatggcttc 
      tcccgtcgc atttggacg tcccggaaa tttatttcc 
      tactaactg cccttataa tgggggtgc cggtcaagc 
      ttttaccag ccgagacaa agttcgatt tggacgcaa 
      tttggacgt ttctctggg gcagctcca taagcaacc 
      aaccggcga ctagtgtgg ccccccgga tttgccttt 
      gtcccgaag ctgttgtcg ttttattga caatggcaa 
      ggtccatgc tccacacca gcgactcga ctttacccg 
      agtggttgc aaaaccacc ccatttcaa tcatccaag 
      ttctgttcc aattgtacc acaacatgc ccgacttta 
      tgcccattt ttatgtcgt ctgaggtgc tcggggcct 
      tggctgggg tcctgttcc ccccacctg tggtctcaa 
      gcatggata gtggggata gtagcggtg cctctactc 
      ccgcgggtg acgagaccg aagttattg tcgtgccga 
      gaactccag acgaggatc cattgacta cgcatccgg 
      tcagacagt ttattatcg attttattc gggttattg 
      tggtcgatt cgttaagtt tgtccacga cagggaaag 
      cttcaccgg ttattgacc tggttttgg atgcgtttc 
      cggtcaatt caaccgcgt ctcaacaaa tttgctacc 
      agacggaaa ttttagtca ctttgatgg accatctcc 
      tagctgcgg atatgctag tgggggacc aggcggcaa 
      tcccggttg tgccactgg ccaatgccc acattcaac 
      acgttacac ctaacgtgt tctatctga cggcgcgga 
      ttgttgata aactgtggc aagggtcct taactaagg 
      acccacatg aagttttcg atttcatag tcaagttaa 
      tcgggcaag caaaacgcc gcaccggta atctctcac 
      ttgagtgtc tctaccatt gtctcaatc gcagtattt 
      tgttcctcc tgtcccgcc ctactgaca ccatcgccg 
      atcaaatgt cggtgttgg aagccttac acaaactga 
      caaccaatc gtttgattg tgttcggag ccggcaatc 
      acgaattag atttcattg ggtgcctta cttactgcc 
      ttctccagt taacagccg atatgaaaa tcatgttgg 
      agtcaaaca tgcgaacaa cctggcatg ttaacccaa 
      gcagtgatc ttgaatcac gcgatgagc aattaaacg 
      cccatcaag ggcgcaatt taatggcag gggcggctt 
      tacgcgtgt gggaagaac tctacgttt gcaaaaaac 
      cagcaattg aggaggttt ttaaagtca cactggcgt 
      tcccgaacc cgggttgca aagcacccg acttgacaa 
      tagtccggg agcaagtgt tcgacgctg cgtccaaac 
      tatggctca actatgtcg acgccgaac gtaaagcaa 
      cctatcgag tcccttggg agttacaca accaaatcc 
      aatcgaaaa tccataaag ttacaggcg gtttaaccc 
      gcttagcca gtcggacta aagtccgaa aggacgaca 
      caatgcgaa tcaaaatca ggatgagca aaaaacaag 
      ccttacaat ggatgataa accacgtga acctcctcc 
      gggaattcg tagaaatgc gccgccaac aacaccaaa 
      acaccaccc aacacaccc agagatacg tccaccaag 
      agtggctcc aaaccgaaa acgagctga cacgggaac 
      ggggacttt ggggccccc ctgagccct gctacctcg 
      acaaatgga tgctggaca tgactgaag ggccccaca 
      ggatgcgca agaagacaa tgaggaacg atctcttct 
      acctgcatt acccctacc ggcccgaac acgagaaag 
      gcttactca agtataaag tcgcaacga gccaccaca 
      gtaaaatct actaccagg cggttcaaa acaaaagaa 
      ccggggtta cgggtttaa ccgaaccag aacgggccg 
      atagtatcg tggatgaac cgtcaccgc tgccactga 
      ccccgcttc cgcccctgg ccaggctca agtcggacc 
      cggacgacc aacaggagg agaatgacg tggaggagg 
      aaccctcag caccgccgg ataaccaaa ccttgatga 
      ggcttctag aagagaacg agaagattt cgcacatgt 
      ttcgctgcg cccgcattc aaggcttgg acacaaccg 
      aagggttgt tgctattcg gacaagaac attgctacg 
      tcgttgatg gtttcactc tgttgaaga atcgcgaaa 
      ttcgggata ttgagggtg tgccgaata acgcgcacg 
      agtacgaac ccattttgc ccaaccctg ggtcgccta 
      gaaggtcgc gcgctatgc gccagtgct ccaaatttt 
      catacgaag acatcagag aggtgcaca agcggcggt 
      acctcctgc agccgatgg ccctggagg tgttttccg 
      gggacggcc ggaactacc tcctggctt tcccccgga 
      aagtccgga acaaggtgt atcagcgtt tcatggcca 
      tggccactg ggatcgctg atagtgccc aacagtcgg 
      tcgcgggtc agcgccgga cggccaatg tcgaacaag 
      caacgagtc ttttaagca atttgattg gcgagacga 
      gtgtgacac aacgctggg tcagaggcg aaatctcaa 
      atttccagt acttgatgt acttgaata ctttagccg 
      gccgttcgt ataacgatt tgatgcagt acccggtgt 
      ttccctgga tcttagccg attatgact cggaagagc 
      tcgacttaa ccgacccga caactttcc gtgacttca 
      ccaaaccga tgggagccc aaagccccg aacgtttgg 
      agcaattcc ggaaacgtt tcaaagaat gggatgacc 
      cacacgggg aaacccctc cgtaccatt atgggacgc 
      actattcta gtgccatga tcgtgtgct cgtatttag 
      tccgaagtg agcctttca cgcaacatt tcctgaggt 
      tacaaggcg gacacttcg atttagccg accgtatgt 
      ttcattcgg tcaaagagg cttggttat cggcctcca 
      aacacaata tggccgatg tgaaggagc tgattcctg 
      aatttttct acctacctg agtttttgg agaacttga 
      atcaatttg atttggggg cgaaacaaa gcggggttg 
      ctttgggtg acgtggtgt taccacctt gggccaagc 
      aggacccac gggagttca taagggttc acagaatgg 
      ccctggagt gtgactacg tgtcacaga atctccacc 
      attggattg acctcattt tcggttaca aacagcaag 
      gacagccgg ctattgact cggttaatg tcttccatc 
      atttgttct acgagggaa acgtcgata tttacaata 
      ttgacgatc cattgaaaa agaatgcaa ctataggtt 
      ttattaata atttcgggc aaattccta aacaattgt 
      tcaagaaac cacaccctt gtaaaaaaa tgggcggag 
      gtaataacc ggacgtcgg ttattgata cggggtccc 
      tagtccaag cgttcactt taccacaac ctgagaaac 
      tctttttcc accacttag acaacctta gaccctcct 
      tacagccaa ttaaagaag tgaaaagtc ccgtcggcc 
      aaatcctcc acgtggata aaacagtcc tcgccgtcg 
      aacaaaaca aagtattaa tcccaacgt tgtacaaca 
      aatacgtgg cttggggct tcgctgacc actccctaa 
      gaatcggga attacgaca gcgataaag ccgtcttcg 
      tgtccgaaa gcgtggtcc aaaacaacg gcgaaatcc 
      tggcctgtg tttgtccct ctcccaaac taatcgtca 
      cagctggga ccagttcag tggtgacaa tctgggaat 
      tcgaatccc ggaaaaaat ccctcaaca tcaaacaca 
      atctctatg cacacacgg acgcagtga cgtgtctcg 
      ctgcaagtc ccgcataca acgtcatag tttcggggt 
      cccgggatt gcaccgcaa tcatttggg acttatccc 
      gagggattt tcgtgcgac agttacggg gtcacgctg 
      gacccatcg ccgcagtcg ccgtgaacg acctcacag 
      gggacattg ccacgtgtg cggccacgt ggcttcacg 
      tctggagca taaacgggg acgagctgg cgagtgtca 
      acacgtgtg tcgggaggg ctgacagaa tgcagcaaa 
      ttctgtgtg tggcgttct cgcaaacgc acgaatggg 
      actcgttga atgtcgtga tgacggggt ccacagatg 
      gggtcacaa ggatgtggt cgattgaat acgtgcaag 
      tgcccgacg agaccaaca ccaacatgg aaccaaagc 
      accagagtc cggtccggc cgactccga tccaccacc 
      gtctagtca agcagatca atgtttcaa acgacagct 
      ctctgagtt tgatgaaat cgggctccg agagcagct 
      tatccgaag acaataata cgaggcgac cttcgatcg 
      atcgtcaag cgagctcca aggagtgga gaagtgagc 
      aacaaaacg aaaaggaaa cgtgcagtg acactgagg 
      agccttcac ctccgatac ctgtgcgga ggcgacggt 
      cacgagtgc atggagggt tcaggaaga gtgaaggtg 
      gtaacagcg ccgataatg ctagtctgg acgtcgacg 
      acggcacca gcatgcgga ccagcaagt ccgacacta 
      gcctgaccg attcgttcg ttatggtgc cagagagta 
      agaaacgaa ctgtgaatc ctatgaacg tcctcaggc 
      cagggttca agtggcagc gccagctgg tcttcagga 
      gcaaactga caatgcaca ccgcctacg accgcaagg 
      acaacgcgg agcccgcct ctatcacag ccctggcgg 
      tctcaaaag accaccgca gcgtctacg tgggagaca 
      cccgcggca gggttttct cttggagcg tctgcgacg 
      ttccgggtc gggcggccg accactggc tcaaggacg 
      aaggagccg acttttgcg cttcgtgca atatcaggt 
      ttacaattt acgaaagga aacaccatt gtcgcaatt 
      gcggacaag tgttttgct cgaaatgca gccgattcg 
      agagcgaaa tctcacggt tgcgtattc tgaaaccgg 
      tgagggttt gtcagacgt gttacgcca ctctcaagt 
      cagaaaaat agagttttt tgtaagtat gttagtgta 
      tagagtacc aaaatagta aacagaaaa cgtattatt 
      tattatgat attgggtgt gattttatt attgttttt 
      atttatgag atattttag ccgttttta acacgtgtt 
      gtattgttt tttattatt tttaataca taaatgttt 
      acatcaggt tttttgtta tgatgtttt tgtgatatt 
      tacattgta gtatttttt atgcatata agtgttttg 
      cttagctaa agtattatt ttgaagcaa agcaatatg 
      tatttagaa caataatat ctgttgcct ggtatactg 
      taaatgttg attttatgg aatttgtgt acaataatg 
      tgtaagata ggtttagtg caattccgt gcgagaaag 
      ataaatttc attatgatt gatattatt tttgtattc 
      aacaatgga ccgaatttg ttagactgt aggtaaaag 
      aggtgtgca ctggcatct gtagacaat atacaagtg 
      cactattgt gcattccaa agtatattt ccttatatt 
      tgataaatg tatgtgata ttgtagatt ctaggttta 
      gcaaattgt aaatatgcc atgtcttga ttatataat 
      aataaaaaa tatagtaac tt
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