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Gene information
General information
TC000886 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7796515..7798176
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000886
    iBeetle screen iB_09623

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000886 RA
      MYSVLHKKE NAHEESIWS CAWGRYTTE KKKKDQNEE 
      EKSRDSVMS DEPPVDYII TGALDDLVK VWELQDDRL 
      VLKHNLEGH SLGVVSVAV SNNGKLCAS SSLDSSMRI 
      WDLERGKKI AHVDVGPVE LWTVAFSPD DKYIISGSH 
      AGKITVYNV ETAKAEQTL DTRGKYILS IAYSPDGKY 
      IASGAIDGI VNIFDVAGN KLWQTLEGH AMPIRSLCF 
      SPDSQLLLT ASDDGHMKL YDVQHTNVV GTLSGHASW 
      VVSVAFSPD GKYFVSGSS DKTVKVWEL ASKQCVHTF 
      KEHNDQVWG VRFSPDSTK IVSVSEDKS INVYSCPV
    CDS sequence
    • >TC000886 RA
      atgtattct gttttacac aagaaggag aatgcgcac 
      gaggaaagc atttggagt tgtgcgtgg ggccggtac 
      accactgaa aaaaagaaa aaagaccag aatgaagag 
      gaaaagtcc cgagattcg gtgatgtcg gacgagcct 
      cccgttgat tacatcata acgggagct ctagacgat 
      ctggtaaaa gtgtgggaa ttgcaagac gatagactt 
      gttttgaaa cataactta gaagggcat tcgttgggg 
      gttgtctcc gttgccgtt agcaacaac gggaaattg 
      tgtgcttcc agttcgtta gactcaagt atgaggatt 
      tgggacttg gaaaggggg aaaaaaatc gcgcatgtt 
      gatgtgggt ccggttgaa ctgtggact gtagcgttc 
      agtcctgac gacaagtac attatttcc ggctcacac 
      gctggaaaa atcacggtt tataacgtg gaaacggcc 
      aaagctgaa caaacttta gatacgaga ggcaaatac 
      attttgagc atagcttat agtcctgac ggtaaatac 
      atagctagt ggggctatt gacggcatt gttaatatt 
      tttgacgtg gcgggaaat aaactatgg caaaccctt 
      gaaggccat gctatgcca atcagatct ctttgtttt 
      tctccagat tctcaacta cttttgact gcgtcagat 
      gacggccat atgaaactt tacgacgtg caacatact 
      aatgttgtt ggtaccttg tcagggcac gcttcttgg 
      gtcgtaagc gttgcattt tctccagat ggtaaatat 
      ttcgtgtca ggaagttcc gataaaact gttaaagtt 
      tgggaattg gcctctaaa caatgtgtt catacgttc 
      aaggaacat aatgatcaa gtttggggt gttaggttt 
      agtccagat agtaccaaa attgtttca gtgtctgaa 
      gataagagt ataaatgtt tacagctgt ccagtttag
    mRNA sequence
    • >TC000886 RA
      caggaattg attttttga aacaaagac gactccctg 
      aggcccttg ggaggcgaa aacttgcat aaaaactct 
      cactgattt gggaaattc aaaatatat attcaataa 
      tttggggaa tttggtgtt tcgggttgg caaagcggc 
      aaaaaccac gctataacc tcacattta ctcgcgcaa 
      tgtattctg ttttacaca agaaggaga atgcgcacg 
      aggaaagca tttggagtt gtgcgtggg gccggtaca 
      ccactgaaa aaaagaaaa aagaccaga atgaagagg 
      aaaagtccc gagattcgg tgatgtcgg acgagcctc 
      ccgttgatt acatcataa cgggagctc tagacgatc 
      tggtaaaag tgtgggaat tgcaagacg atagacttg 
      ttttgaaac ataacttag aagggcatt cgttggggg 
      ttgtctccg ttgccgtta gcaacaacg ggaaattgt 
      gtgcttcca gttcgttag actcaagta tgaggattt 
      gggacttgg aaaggggga aaaaaatcg cgcatgttg 
      atgtgggtc cggttgaac tgtggactg tagcgttca 
      gtcctgacg acaagtaca ttatttccg gctcacacg 
      ctggaaaaa tcacggttt ataacgtgg aaacggcca 
      aagctgaac aaactttag atacgagag gcaaataca 
      ttttgagca tagcttata gtcctgacg gtaaataca 
      tagctagtg gggctattg acggcattg ttaatattt 
      ttgacgtgg cgggaaata aactatggc aaacccttg 
      aaggccatg ctatgccaa tcagatctc tttgttttt 
      ctccagatt ctcaactac ttttgactg cgtcagatg 
      acggccata tgaaacttt acgacgtgc aacatacta 
      atgttgttg gtaccttgt cagggcacg cttcttggg 
      tcgtaagcg ttgcatttt ctccagatg gtaaatatt 
      tcgtgtcag gaagttccg ataaaactg ttaaagttt 
      gggaattgg cctctaaac aatgtgttc atacgttca 
      aggaacata atgatcaag tttggggtg ttaggttta 
      gtccagata gtaccaaaa ttgtttcag tgtctgaag 
      ataagagta taaatgttt acagctgtc cagtttagt 
      ttacacttc gtaatttat taattaatt gatttagtg 
      aagtgtatt gcttaaaca cctagttgt ataattttt 
      tttataata aagctattt ttacatcca ttgttggta 
      ttattgcta ccacattac ttctaaaat ttcctactt 
      cctacttcc atattaatt taataattt tgtggtgtt 
      tct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY