Status
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Gene information
General information
TC000677 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7632807..7639489
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000677
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09622

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000677 RA
      MLVGSTNNE LPESWKSCS ISFVSADLQ HLVINLDST 
      SVVVQVIPE HDIIKEIII TKIFAISVS LVEGSVFFK 
      LYFCNLPLP KFNKKVGSC VQTVLTLLG YTPECPEDV 
      GDNDVDMSS NCTLTQLYK NIRKTRETH LNLGCADVQ 
      DSRLKPQLR PYQADAVRW MLYREKKLE DAQDELHPL 
      YSVIKLKSG LEIYFDKYS GFVDTTKPI VDPSSRGGI 
      LADEMGLGK TVEVLACIL LHPKPDGAP KPLDLEKTP 
      TVDLVNRKR RILTQKPVS TPQKLKISE SCVKPSGRK 
      SEKYIALEM WYNSVLQSA VPKVAPSPP EPALQCICG 
      GADDEGSVT CTECGKMQH GACLGYSTQ LGPYICPQC 
      WLSQPQIEC KATLIVSPI SLRTQWCKE ICRHVRGDF 
      KVLQYGGSS VTPVYPTAL TSYDVVITT YNVLQTELK 
      LTETEKALS LRHQRRYSA PGSPLTTVK WWRLCLDEA 
      QTVETPTSM VSAMAKKLN AYFRWAVTG TPISKDISD 
      LHGLIDYLQ IEPYNDKFT WEQLLFKLY VRGNPEPML 
      KFLSEVLWR SSKDEIIDQ INIPKQTII EHWLSFSAV 
      EQYFYKCEH QTSREAFSN KVRTYEPDL PLTSLDRSS 
      LKSVLAPLL SLRQVCTYP NSAHTKYLI TKKPVKSMK 
      DLLDALIAR NVNECEEHL RVILSSLNG LAGIYLLLE 
      APEQAIEQY REVLQLYTR FTEEEKISK LNVDKLQVV 
      HTMHNLAEI LDANPGKFA RTLRDENLR KDCADLEQK 
      YIERFINQS MAVLQDVLL ISGNIEKLQ NSFILAPGK 
      WYSDLLDWI YIHSYEQEL HTKIMNLHS DANVKCTVE 
      SQSPRTLIY CIATWDENI SELRETTIE VVNDLYTHC 
      PDDEFKIII AQELVQKAT DCHLRPQKK SKSRKKCPV 
      CVANDHLKA YELKLFAMS KRTETFVDM SLKGSWKPT 
      PEELIFKSI LAVGRSKNA DATLLKDGE VHINILETL 
      KKEFKEVRK LWTYLEQQI CAQDELDMC KIRLRLKEA 
      NEEEKQNKI LQNLNYNIV NKLETIHVL DVHEVGYQR 
      TVLHSEENR NKALLEKNL GTRSYLETL RTQQYEDQP 
      PDPCPICKN CLEKQWSIL SCGHCYCLE CIQLLIDQT 
      KGHHVQCSV CRSYHLTSE ISYIKPGEH QTVPESEKI 
      AGNYSTKVE SIVRLILKL RLEDEDVKI LLFSTWIPV 
      LSYIREALT KNSVTSELI TSGNLEKQI EKFKDADQN 
      ITVLLLPIN LGGKGLNLI EATHVILTE PLLNPGEEL 
      QAIGRVHRI GQTRPTVVH KFFIKHTIE ESIQKAVST 
      DAKNWEKNK VTLGQLVDL FVTDDASEE QQNENSDL
    CDS sequence
    • >TC000677 RA
      atgctagtt ggaagcaca aataatgaa cttccagaa 
      agttggaag tcttgcagt attagtttt gtgagtgca 
      gatttacaa catttggtt attaacttg gattccacg 
      tccgttgta gttcaagta ataccagaa cacgatata 
      attaaagaa attataatt acgaaaatt tttgcgatc 
      agtgtgagt cttgttgag ggtagtgtg ttttttaag 
      ctttacttc tgtaattta ccactgcca aaatttaat 
      aaaaaagta ggatcttgt gttcaaaca gtattaacg 
      cttttggga tacactccg gagtgtcct gaagatgta 
      ggtgataat gatgtagat atgtccagt aattgcaca 
      ctcactcaa ttgtataaa aatatacga aaaacacgc 
      gaaacacac ttaaatctt ggctgtgct gacgttcaa 
      gactcgaga cttaagcct cagctcagg ccttatcag 
      gccgatgcg gttcggtgg atgttatac agagagaag 
      aaattggag gatgctcaa gacgagctg catccttta 
      tattctgtg ataaaacta aaatcgggc ctcgaaatt 
      tattttgat aaatattca ggctttgtt gacaccacc 
      aaaccaatc gtagaccca tcaagcagg ggcggtatt 
      ttagcagac gagatgggt ttaggcaaa actgtggaa 
      gttctcgcc tgcatcctc ctccacccg aaacctgat 
      ggagccccc aagcccctt gatctggag aaaacccca 
      actgtggac ctcgtaaac cgaaaacgc cgaatcctg 
      acccaaaaa ccagttagc acaccccaa aaattaaaa 
      atctcggaa agttgcgtc aaacccagt ggaagaaaa 
      tcagaaaag tatattgct ctcgaaatg tggtacaac 
      tcggttctg cagagcgcc gtgcccaag gtcgccccc 
      agcccgccg gagcccgcc ctccagtgc atatgcggg 
      ggcgcggac gacgagggc agcgtcact tgcaccgag 
      tgcggcaaa atgcagcac ggggcttgt ctggggtac 
      agcacacag ctggggcct tacatatgc ccccagtgt 
      tggctgagc cagccccaa atcgaatgc aaagccacc 
      ctcattgtc tcgcctatc tctttgcga acgcagtgg 
      tgtaaggag atctgcagg catgtgagg ggcgatttc 
      aaggttttg caatatggg ggctcctca gtcactccg 
      gtgtaccca actgcgttg actagttac gacgttgtg 
      ataaccacg tacaatgtt ttgcagact gagttaaaa 
      ttaaccgaa actgagaaa gctttgtct ttgagacat 
      cagcgccgg tattcggcc cctgggagt cccctaact 
      acggtcaag tggtggcgg ctgtgtttg gacgaagca 
      caaactgtt gagacgcca acaagcatg gtttcagca 
      atggccaag aaattaaac gcttatttc aggtgggcc 
      gtcactgga acccccatt tcaaaggac atttcagat 
      ttgcacggc ctgatcgat tacctacaa atagagccg 
      tacaatgac aagtttacg tgggaacag ttattattt 
      aagctgtac gttcgggga aaccccgag ccaatgctc 
      aaatttcta tctgaagta ctatggcgt tcttctaag 
      gacgaaatt attgatcaa ataaatatc ccgaaacaa 
      acaataata gagcactgg ttgagtttt tccgctgtt 
      gagcagtac ttttataaa tgtgagcac caaacaagc 
      cgtgaggca ttttcgaat aaagtcagg acttacgaa 
      ccggatttg cctttgaca tcattggat agaagttca 
      ttgaaaagt gttcttgca cctttattg tcccttcgt 
      caagtttgt acgtacccg aattcggca cacaccaag 
      tatttaata acgaaaaaa ccagtcaaa tcgatgaag 
      gatctcctt gacgcactt atagcccgt aatgtaaat 
      gagtgtgaa gagcatttg cgagttatt ttgagttct 
      ttaaacgga cttgccggt atttatttg ctcctcgaa 
      gctccagaa caagcaatt gagcagtac cgtgaggtg 
      ttacagtta tacacccgt tttactgaa gaagaaaaa 
      atttctaaa ttaaatgtt gataagtta caagtcgtc 
      cacactatg cataatctc gcggaaatt ctcgatgcg 
      aatccggga aaattcgcc aggaccttg cgagatgaa 
      aacctgcga aaggactgt gcagatcta gagcaaaaa 
      tatatagag cggtttatc aaccagagt atggctgtc 
      cttcaggac gttttatta atttccgga aacattgaa 
      aaactgcaa aatagtttt atcctcgcc cccgggaaa 
      tggtacagc gatttgttg gactggatc tacatccac 
      agctacgag caagaactg cacaccaaa atcatgaat 
      ttgcacagc gatgccaac gtgaaatgc accgtagag 
      tcccaaagt cccagaaca ttgatttat tgcattgcc 
      acttgggac gaaaacata tcagaactg cgcgaaacc 
      acaatcgaa gtcgtcaat gatttatac acccactgt 
      cctgacgac gaatttaaa ataataatc gctcaagag 
      ttggtacaa aaagccaca gactgccac ctccgccct 
      cagaaaaag tccaaaagt aggaaaaaa tgcccggtt 
      tgtgtggct aacgaccac ctgaaagcc tacgaactc 
      aagttattc gcaatgtcg aaacgaacg gagactttc 
      gtggatatg tcgcttaag gggagctgg aaaccgaca 
      ccggaagaa ctcattttc aaatcaata ctagcggtc 
      ggccggtcg aagaacgct gacgccact ttgttaaaa 
      gacggtgaa gttcatata aatatttta gaaaccctc 
      aagaaagag tttaaagaa gtgagaaag ttgtggacg 
      tacctagag caacaaata tgtgcacag gacgagctg 
      gacatgtgc aaaattcgc ttgaggctc aaggaagct 
      aacgaggag gagaagcaa aacaaaatt ttgcaaaac 
      ttgaattac aatattgtg aataagttg gagactatt 
      cacgttttg gacgtgcac gaggtcggt taccaaaga 
      accgtttta cattcggag gagaaccgg aacaaggct 
      ctcctagag aaaaattta gggacaagg agttacttg 
      gagacgttg cggacgcaa cagtatgag gatcaaccg 
      cctgaccct tgccccatc tgcaaaaat tgtttggag 
      aagcagtgg tcgattttg tcgtgtggc cattgctat 
      tgtttggag tgcatccaa cttctgatt gatcagacc 
      aagggtcac cacgtgcaa tgctccgtt tgtcgttcg 
      tatcaccta acaagtgag atttcttat ataaaaccg 
      ggggagcac caaaccgtc cccgagagt gaaaaaatc 
      gccgggaat tactcaaca aaagtcgaa agtatcgtc 
      cgtttaatt ttaaaattg agactcgaa gatgaggac 
      gtcaagatc ttgcttttt tcgacttgg atccctgtt 
      ttgtcgtac attagggaa gccttgact aagaatagt 
      gtcacgtcc gaattaatc acttctggt aatttggaa 
      aaacagatt gaaaaattt aaggatgct gatcaaaat 
      attacggtc ctccttttg cctataaat ctcggcggt 
      aagggactt aacttgatt gaagccact catgtaata 
      ctgaccgag cctttgctc aatccgggc gaggagctt 
      caagctatt ggccgagtt catcggatt ggccagacc 
      agaccgact gttgtccac aagtttttc ataaaacac 
      acgattgag gaaagtata cagaaggca gtaagcacc 
      gatgcgaaa aattgggag aagaataaa gttacccta 
      gggcagttg gtagatttg tttgtcacc gatgatgct 
      tccgaagaa caacaaaat gaaaattcc gacttgtag
    mRNA sequence
    • >TC000677 RA
      tcataattt ttgttggcg actatgtta tctgactta 
      tcgcttgtg tttttgtaa cctccaatt tcaagttca 
      ttttttata acaaaaaat ctaatttaa agctagttg 
      ttagaagat aaaatttct agataaaaa ataattgtt 
      tacaatggg tcgacaaaa gaaccagcc tgcaaaatt 
      aaaaaacac agatgaaga ttttggctt tcgaaagcc 
      aatagatta cattccaat taaaaaacg aagaaataa 
      gaaaggaaa aggttcttc aggaccttg ttataaaat 
      aattttacc ttctggccg aaacgaaga gttttattt 
      gggggacat gctagttgg aagcacaaa taatgaact 
      tccagaaag ttggaagtc ttgcagtat tagttttgt 
      gagtgcaga tttacaaca tttggttat taacttgga 
      ttccacgtc cgttgtagt tcaagtaat accagaaca 
      cgatataat taaagaaat tataattac gaaaatttt 
      tgcgatcag tgtgagtct tgttgaggg tagtgtgtt 
      ttttaagct ttacttctg taatttacc actgccaaa 
      atttaataa aaaagtagg atcttgtgt tcaaacagt 
      attaacgct tttgggata cactccgga gtgtcctga 
      agatgtagg tgataatga tgtagatat gtccagtaa 
      ttgcacact cactcaatt gtataaaaa tatacgaaa 
      aacacgcga aacacactt aaatcttgg ctgtgctga 
      cgttcaaga ctcgagact taagcctca gctcaggcc 
      ttatcaggc cgatgcggt tcggtggat gttatacag 
      agagaagaa attggagga tgctcaaga cgagctgca 
      tcctttata ttctgtgat aaaactaaa atcgggcct 
      cgaaattta ttttgataa atattcagg ctttgttga 
      caccaccaa accaatcgt agacccatc aagcagggg 
      cggtatttt agcagacga gatgggttt aggcaaaac 
      tgtggaagt tctcgcctg catcctcct ccacccgaa 
      acctgatgg agcccccaa gccccttga tctggagaa 
      aaccccaac tgtggacct cgtaaaccg aaaacgccg 
      aatcctgac ccaaaaacc agttagcac accccaaaa 
      attaaaaat ctcggaaag ttgcgtcaa acccagtgg 
      aagaaaatc agaaaagta tattgctct cgaaatgtg 
      gtacaactc ggttctgca gagcgccgt gcccaaggt 
      cgcccccag cccgccgga gcccgccct ccagtgcat 
      atgcggggg cgcggacga cgagggcag cgtcacttg 
      caccgagtg cggcaaaat gcagcacgg ggcttgtct 
      ggggtacag cacacagct ggggcctta catatgccc 
      ccagtgttg gctgagcca gccccaaat cgaatgcaa 
      agccaccct cattgtctc gcctatctc tttgcgaac 
      gcagtggtg taaggagat ctgcaggca tgtgagggg 
      cgatttcaa ggttttgca atatggggg ctcctcagt 
      cactccggt gtacccaac tgcgttgac tagttacga 
      cgttgtgat aaccacgta caatgtttt gcagactga 
      gttaaaatt aaccgaaac tgagaaagc tttgtcttt 
      gagacatca gcgccggta ttcggcccc tgggagtcc 
      cctaactac ggtcaagtg gtggcggct gtgtttgga 
      cgaagcaca aactgttga gacgccaac aagcatggt 
      ttcagcaat ggccaagaa attaaacgc ttatttcag 
      gtgggccgt cactggaac ccccatttc aaaggacat 
      ttcagattt gcacggcct gatcgatta cctacaaat 
      agagccgta caatgacaa gtttacgtg ggaacagtt 
      attatttaa gctgtacgt tcggggaaa ccccgagcc 
      aatgctcaa atttctatc tgaagtact atggcgttc 
      ttctaagga cgaaattat tgatcaaat aaatatccc 
      gaaacaaac aataataga gcactggtt gagtttttc 
      cgctgttga gcagtactt ttataaatg tgagcacca 
      aacaagccg tgaggcatt ttcgaataa agtcaggac 
      ttacgaacc ggatttgcc tttgacatc attggatag 
      aagttcatt gaaaagtgt tcttgcacc tttattgtc 
      ccttcgtca agtttgtac gtacccgaa ttcggcaca 
      caccaagta tttaataac gaaaaaacc agtcaaatc 
      gatgaagga tctccttga cgcacttat agcccgtaa 
      tgtaaatga gtgtgaaga gcatttgcg agttatttt 
      gagttcttt aaacggact tgccggtat ttatttgct 
      cctcgaagc tccagaaca agcaattga gcagtaccg 
      tgaggtgtt acagttata cacccgttt tactgaaga 
      agaaaaaat ttctaaatt aaatgttga taagttaca 
      agtcgtcca cactatgca taatctcgc ggaaattct 
      cgatgcgaa tccgggaaa attcgccag gaccttgcg 
      agatgaaaa cctgcgaaa ggactgtgc agatctaga 
      gcaaaaata tatagagcg gtttatcaa ccagagtat 
      ggctgtcct tcaggacgt tttattaat ttccggaaa 
      cattgaaaa actgcaaaa tagttttat cctcgcccc 
      cgggaaatg gtacagcga tttgttgga ctggatcta 
      catccacag ctacgagca agaactgca caccaaaat 
      catgaattt gcacagcga tgccaacgt gaaatgcac 
      cgtagagtc ccaaagtcc cagaacatt gatttattg 
      cattgccac ttgggacga aaacatatc agaactgcg 
      cgaaaccac aatcgaagt cgtcaatga tttatacac 
      ccactgtcc tgacgacga atttaaaat aataatcgc 
      tcaagagtt ggtacaaaa agccacaga ctgccacct 
      ccgccctca gaaaaagtc caaaagtag gaaaaaatg 
      cccggtttg tgtggctaa cgaccacct gaaagccta 
      cgaactcaa gttattcgc aatgtcgaa acgaacgga 
      gactttcgt ggatatgtc gcttaaggg gagctggaa 
      accgacacc ggaagaact cattttcaa atcaatact 
      agcggtcgg ccggtcgaa gaacgctga cgccacttt 
      gttaaaaga cggtgaagt tcatataaa tattttaga 
      aaccctcaa gaaagagtt taaagaagt gagaaagtt 
      gtggacgta cctagagca acaaatatg tgcacagga 
      cgagctgga catgtgcaa aattcgctt gaggctcaa 
      ggaagctaa cgaggagga gaagcaaaa caaaatttt 
      gcaaaactt gaattacaa tattgtgaa taagttgga 
      gactattca cgttttgga cgtgcacga ggtcggtta 
      ccaaagaac cgttttaca ttcggagga gaaccggaa 
      caaggctct cctagagaa aaatttagg gacaaggag 
      ttacttgga gacgttgcg gacgcaaca gtatgagga 
      tcaaccgcc tgacccttg ccccatctg caaaaattg 
      tttggagaa gcagtggtc gattttgtc gtgtggcca 
      ttgctattg tttggagtg catccaact tctgattga 
      tcagaccaa gggtcacca cgtgcaatg ctccgtttg 
      tcgttcgta tcacctaac aagtgagat ttcttatat 
      aaaaccggg ggagcacca aaccgtccc cgagagtga 
      aaaaatcgc cgggaatta ctcaacaaa agtcgaaag 
      tatcgtccg tttaatttt aaaattgag actcgaaga 
      tgaggacgt caagatctt gcttttttc gacttggat 
      ccctgtttt gtcgtacat tagggaagc cttgactaa 
      gaatagtgt cacgtccga attaatcac ttctggtaa 
      tttggaaaa acagattga aaaatttaa ggatgctga 
      tcaaaatat tacggtcct ccttttgcc tataaatct 
      cggcggtaa gggacttaa cttgattga agccactca 
      tgtaatact gaccgagcc tttgctcaa tccgggcga 
      ggagcttca agctattgg ccgagttca tcggattgg 
      ccagaccag accgactgt tgtccacaa gtttttcat 
      aaaacacac gattgagga aagtataca gaaggcagt 
      aagcaccga tgcgaaaaa ttgggagaa gaataaagt 
      taccctagg gcagttggt agatttgtt tgtcaccga 
      tgatgcttc cgaagaaca acaaaatga aaattccga 
      cttgtagtt tttgtactt atttcaatg tttcaataa 
      agttttaca ttttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY