Status
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Gene information
General information
TC032070
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000843,TC030305,TC000842
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6671845..6678063
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032070
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09616

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032070 RA
      MLETKQIVF HSFKLNVTV LSLIGLYPP KNYSILYKI 
      YAVILFLAV HTPQLVLGL LHYFLMGDF TSIDYSDFV 
      TVGMMFYAF KLLPFVTSV TKIQKCINY FDTLGYKIL 
      KSEEKIIED CVGSCRRNT NVFFVGCCL SWMGFVAQV 
      FLRDEPQQL PLKVWFPYS RDESPVLFY CIYILLIFG 
      PGYSVLACG TIDPMIGGL AYHAAAQLQ RLKRNLQYL 
      DEYIKEKNV GKSKENKRG VIYEEIISC VQRYQEIAT 
      FVDLFKDSF SQVVFSQFM GSVFLIGLC CFQIITATE 
      VDINFVITA NYIWVILFQ IFFYCYYGT MLIEENYTL 
      TNAIYLSNW YEYSIPEQK ALFMLMERS KKPMIVTAG 
      KILDLSLDT FTMILRRSI LLMHSFFNI KVHNKMPVT 
      VTDCFGVNL TVMGLFGMY HRSGNPTIF EKVIAYCMF 
      FFFTIPIPI LGSLYFFFQ ENVDLEELH NNGFLIAEM 
      VCNLAKYLS FIKNGHRIR KCIHYLELP IFATRRENQ 
      ERIINASGW ICKRNSRVF FVSVLAANV FWAGRPFME 
      NKQKFPIEI WLPFDAKAN AMAFYLIYL FLVIAVAYA 
      SIACAVIDP LIAGLASLA SGQVEVLKD NLENLSKYT 
      REQMLEKGI ICNKFDEVF YANVRKCVD HHNAILHFI 
      KEYEECFSS SVFSQFLGS ILVICFCCL ELTKIEPLS 
      FNFYSMTFF ATCFSAQIY MYCYYGTVL YDESNSIAS 
      AIYMGTWYE YDVKSRKAL INLMERSKK PIHLRAGKV 
      LEMSVETFT MILRRSYSL LALEPLSYE FLQAIIYMV 
      CVVVQIYFY CYYGTILYE ESNSLNDAV YMGNWYKYD 
      IKSRKTLLI LMERSKKPC IVTAGKVLD LSLVTFTTI 
      LRRSYSLLA VLKNQK
    CDS sequence
    • >TC032070 RA
      atgctcgaa acaaagcag attgttttt cacagtttc 
      aaactcaat gtgactgta cttagttta atcggcctc 
      tatcctcca aaaaactac tcaattctg tacaagata 
      tatgcagtt attttgttt cttgcagta catacgccc 
      caattagta ctcggcctg cttcattat ttcttgatg 
      ggagacttc acttccatc gattatagc gattttgtg 
      accgttgga atgatgttc tacgctttc aagctattg 
      ccatttgtt acaagtgtc acgaaaatc cagaaatgt 
      attaattat ttcgacacc ctcggttac aaaatcctc 
      aaaagtgaa gagaaaatc attgaggat tgtgttggc 
      agttgtcgt cgcaacaca aacgtcttt ttcgttggt 
      tgttgtcta agttggatg ggttttgtt gcacaagtt 
      tttctaaga gatgagcct cagcagtta ccacttaaa 
      gtgtggttt ccctatagc agggacgaa agtcctgtg 
      cttttttat tgcatttac attcttctt atttttgga 
      cctggttat tctgttctt gcttgtggg acaattgat 
      cccatgatt ggaggtctg gcttatcac gccgctgct 
      caacttcag cgcctcaaa aggaatttg caatatctg 
      gatgagtat attaaggaa aaaaatgta ggtaaaagt 
      aaagaaaac aagcgtggc gttatttat gaagaaata 
      attagttgt gtacaacgg tatcaggaa atagcaact 
      tttgtcgac ttgtttaaa gactctttc tcacaggtc 
      gtgtttagc caatttatg ggaagtgtt tttctcatc 
      ggtttgtgt tgctttcaa attataaca gcgacggag 
      gttgatata aactttgtc ataactgcg aactacatt 
      tgggtcata ttatttcag atatttttt tattgttac 
      tacgggaca atgttgata gaggagaat tatacacta 
      acaaatgcc atttatttg agcaattgg tacgagtat 
      agcattcca gaacaaaag gcacttttt atgttaatg 
      gaacgctca aaaaaaccg atgattgtg accgctgga 
      aaaatttta gatctgtct ttggacaca ttcacaatg 
      atattaaga cgatcaatt cttttaatg cattcgttt 
      ttcaacatc aaagtgcat aacaagatg ccagtaaca 
      gtcacagat tgttttggg gtgaatcta acggtgatg 
      ggtctcttt gggatgtac catcgaagc ggtaaccca 
      acaattttc gaaaaagtg atcgcctat tgcatgttc 
      tttttcttc acaattccc atacccatt ctgggctct 
      ctctatttt ttcttccaa gaaaacgtt gatttggag 
      gaattgcac aataacggc tttctgatc gccgaaatg 
      gtttgtaat cttgcaaaa tatttatca ttcataaaa 
      aatggacac agaatcaga aaatgcatt cattacctc 
      gaattgcca attttcgcc acaaggaga gaaaatcag 
      gaaagaatt attaacgcc agcggttgg atttgcaaa 
      cgaaattcg agagttttt ttcgtttcg gtattagct 
      gctaatgtt ttttgggct gggagacca tttatggaa 
      aacaagcag aagtttccg attgaaatt tggctaccg 
      tttgatgct aaagcaaat gcgatggct ttttatttg 
      atctatttg tttctggtc attgctgtg gcttatgct 
      tctattgca tgtgctgta atagacccg ttgatagca 
      gggcttgca agcttggct tcaggccag gttgaggtc 
      ttgaaagac aatttggaa aatttgagc aaatataca 
      agagagcaa atgctggaa aaaggtata atttgtaac 
      aagttcgat gaagtgttt tatgcaaat gtgaggaaa 
      tgtgtcgat catcataat gcaatttta cattttata 
      aaggaatac gaagagtgt ttctcctcg tctgttttt 
      agccaattt ttagggagc atccttgtt atttgtttt 
      tgttgcttg gaattaact aaaatcgaa cccttaagt 
      ttcaacttc tattccatg acgtttttc gcgacatgt 
      ttttcagct caaatttat atgtattgt tattatggt 
      actgtctta tacgatgag agtaacagc attgcaagt 
      gctatttac atgggcaca tggtacgaa tatgatgtg 
      aagtcgaga aaagctcta attaattta atggaacgt 
      tctaaaaaa cccatccat cttagagct ggaaaagtc 
      cttgaaatg tcagtggag acattcacg atgattttg 
      agaagatct tactctctc ctagctttg gaaccgttg 
      agttacgag tttttgcaa gcaattatt tacatggtg 
      tgtgtggtc gtgcagatt tatttctat tgttattat 
      ggaactatc ctttacgaa gagagcaat agtttaaat 
      gacgcagtg tacatggga aattggtac aaatatgat 
      atcaaatca aggaaaacg ttattaatt ttaatggaa 
      cgttccaag aaaccatgt attgttaca gcaggcaaa 
      gttttagat ttatcatta gttactttt acaacaata 
      ttacgacgg tcatactca ctgttggca gtcctgaaa 
      aaccaaaaa taa
    mRNA sequence
    • >TC032070 RA
      aatcagaac aatgctcga aacaaagca gattgtttt 
      tcacagttt caaactcaa tgtgactgt acttagttt 
      aatcggcct ctatcctcc aaaaaacta ctcaattct 
      gtacaagat atatgcagt tattttgtt tcttgcagt 
      acatacgcc ccaattagt actcggcct gcttcatta 
      tttcttgat gggagactt cacttccat cgattatag 
      cgattttgt gaccgttgg aatgatgtt ctacgcttt 
      caagctatt gccatttgt tacaagtgt cacgaaaat 
      ccagaaatg tattaatta tttcgacac cctcggtta 
      caaaatcct caaaagtga agagaaaat cattgagga 
      ttgtgttgg cagttgtcg tcgcaacac aaacgtctt 
      tttcgttgg ttgttgtct aagttggat gggttttgt 
      tgcacaagt ttttctaag agatgagcc tcagcagtt 
      accacttaa agtgtggtt tccctatag cagggacga 
      aagtcctgt gctttttta ttgcattta cattcttct 
      tatttttgg acctggtta ttctgttct tgcttgtgg 
      gacaattga tcccatgat tggaggtct ggcttatca 
      cgccgctgc tcaacttca gcgcctcaa aaggaattt 
      gcaatatct ggatgagta tattaagga aaaaaatgt 
      aggtaaaag taaagaaaa caagcgtgg cgttattta 
      tgaagaaat aattagttg tgtacaacg gtatcagga 
      aatagcaac ttttgtcga cttgtttaa agactcttt 
      ctcacaggt cgtgtttag ccaatttat gggaagtgt 
      ttttctcat cggtttgtg ttgctttca aattataac 
      agcgacgga ggttgatat aaactttgt cataactgc 
      gaactacat ttgggtcat attatttca gatattttt 
      ttattgtta ctacgggac aatgttgat agaggagaa 
      ttatacact aacaaatgc catttattt gagcaattg 
      gtacgagta tagcattcc agaacaaaa ggcactttt 
      tatgttaat ggaacgctc aaaaaaacc gatgattgt 
      gaccgctgg aaaaatttt agatctgtc tttggacac 
      attcacaat gatattaag acgatcaat tcttttaat 
      gcattcgtt tttcaacat caaagtgca taacaagat 
      gccagtaac agtcacaga ttgttttgg ggtgaatct 
      aacggtgat gggtctctt tgggatgta ccatcgaag 
      cggtaaccc aacaatttt cgaaaaagt gatcgccta 
      ttgcatgtt ctttttctt cacaattcc catacccat 
      tctgggctc tctctattt tttcttcca agaaaacgt 
      tgatttgga ggaattgca caataacgg ctttctgat 
      cgccgaaat ggtttgtaa tcttgcaaa atatttatc 
      attcataaa aaatggaca cagaatcag aaaatgcat 
      tcattacct cgaattgcc aattttcgc cacaaggag 
      agaaaatca ggaaagaat tattaacgc cagcggttg 
      gatttgcaa acgaaattc gagagtttt tttcgtttc 
      ggtattagc tgctaatgt tttttgggc tgggagacc 
      atttatgga aaacaagca gaagtttcc gattgaaat 
      ttggctacc gtttgatgc taaagcaaa tgcgatggc 
      tttttattt gatctattt gtttctggt cattgctgt 
      ggcttatgc ttctattgc atgtgctgt aatagaccc 
      gttgatagc agggcttgc aagcttggc ttcaggcca 
      ggttgaggt cttgaaaga caatttgga aaatttgag 
      caaatatac aagagagca aatgctgga aaaaggtat 
      aatttgtaa caagttcga tgaagtgtt ttatgcaaa 
      tgtgaggaa atgtgtcga tcatcataa tgcaatttt 
      acattttat aaaggaata cgaagagtg tttctcctc 
      gtctgtttt tagccaatt tttagggag catccttgt 
      tatttgttt ttgttgctt ggaattaac taaaatcga 
      acccttaag tttcaactt ctattccat gacgttttt 
      cgcgacatg tttttcagc tcaaattta tatgtattg 
      ttattatgg tactgtctt atacgatga gagtaacag 
      cattgcaag tgctattta catgggcac atggtacga 
      atatgatgt gaagtcgag aaaagctct aattaattt 
      aatggaacg ttctaaaaa acccatcca tcttagagc 
      tggaaaagt ccttgaaat gtcagtgga gacattcac 
      gatgatttt gagaagatc ttactctct cctagcttt 
      ggaaccgtt gagttacga gtttttgca agcaattat 
      ttacatggt gtgtgtggt cgtgcagat ttatttcta 
      ttgttatta tggaactat cctttacga agagagcaa 
      tagtttaaa tgacgcagt gtacatggg aaattggta 
      caaatatga tatcaaatc aaggaaaac gttattaat 
      tttaatgga acgttccaa gaaaccatg tattgttac 
      agcaggcaa agttttaga tttatcatt agttacttt 
      tacaacaat attacgacg gtcatactc actgttggc 
      agtcctgaa aaaccaaaa ataaaacat tggcttatg 
      aactggttt tatggtatt tttaatttt ttggcaaga 
      aataatgta atgtaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY