Status
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Gene information
General information
TC032058
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000816
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6206676..6212750
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032058
    iBeetle screen iB_09612

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032058 RA
      MSGNIFTVT TNTKMTVPA DFETAITAT GFGKFNFLF 
      LLLILASGW AATLESSTM AYVFPAAQC DLDLSLEDK 
      GLLNAITYS GMISSAVIW GFLFDTLGR RKLLMIGFF 
      LDFICVVIS SLSQNKEVL IASKFFGGF IMNGPYAAL 
      STYVSEFHS NNYRGRMQL MIAMSFCCG GIVLPLLAW 
      GILPQELNL QISNTFTIY SWNILLFLC SIPSLVSAI 
      IFVFVPESP KFLMTIGEN EKALKIFQK IYAKNSGQP 
      PKTFPITEL VDEVSLKKK DPNEHGGQV TAKRSQSQA 
      LQEGWQQVK PLFFPPHLK NIILVCLIQ TLFTMTVNT 
      MRLWLPQIF QALNDYQYY YNVSSADLC ESLEMIKSS 
      NVTSDECQV NSDNSSVYI NALIVAVVT LVGDLGASS 
      LINKVGKKT LLFVLGILA GGFEITMYF SMNTAFAVA 
      FSSLFATFT HIAANVCIV VIINLFPTT LRTMSLALA 
      LMCGRIGSM VGNLVFPLL IRTGCGPPF FTIAALVFA 
      CAFLSLLLP NTDSKALEC IIMSGNIFT VTTTTKTTV 
      PADFETAIT ATDFGKFNF YFVLLILVS GWACGFESS 
      TMAYILPVA QCDLDLSLE DKGLLNAIT YSGMISSAV 
      IWGFLFDTL GRRKLLMIG FSLDFICVV ISSLSQNKE 
      VLLASNFFG GFIINGPYA ALSTYVSEF HSNKYRGRM 
      QLMIAVSFS CGGIVLPLL AWGILPQEL NLKISDTFT 
      IYSWNILLF LYSIPSLVS AIIFVFVPE SPKFLMTVG 
      ENEKALKIF QKIYSINSG QPPKTFPIT ELVDEVSLK 
      KKDPNEHGG QVTAKRSQS QALQEGWQQ IKPLFFPPH 
      LKNIIFVCL IQTLFTMTV NTMRLWLPQ IFQALNDYQ 
      YYYNVSSAD LCESLEMIK SSNVTSDEC QVNSDNSSV 
      YTNGLIVAV VTLVGDFSA SFFINKVGK KTLLFVLGI 
      LAGGFEITI YFSMNTAFA VAFSSLFAT FTHIAANVC 
      IVVIINLFP TTLRTMSLA LALMCARIG SMTGNLIFP 
      LLIRTGCGP PFFTIAALV FACAFLSLL LPNTDSKAL 
      E
    CDS sequence
    • >TC032058 RA
      atgtctgga aacattttc acagttaca acaaacacc 
      aaaatgaca gttccagcc gattttgaa accgctatt 
      acagcaacc ggtttcggc aaatttaat tttttattt 
      cttttgcta atccttgcg tcaggatgg gctgcaact 
      ttggaaagt agcacaatg gcttatgtt tttccagca 
      gcacagtgt gaccttgat ttgagtctt gaagataaa 
      ggattattg aatgctatt acatactct ggaatgatt 
      tcaagtgct gttatttgg ggattcctc tttgatacc 
      cttggacgc agaaaactt ctaatgatt gggtttttc 
      ctggacttt atttgcgta gtcataagt tccttatcc 
      caaaacaaa gaagtgttg atagcatcg aaattcttt 
      ggtggcttt attatgaat ggcccttat gccgctcta 
      tcaacttat gtttctgaa tttcactca aataattat 
      agaggacga atgcaatta atgatagca atgagtttt 
      tgttgcgga ggcatcgtc ttacccctt ctagcttgg 
      ggtattttg ccacaagaa ttaaatttg caaatatcc 
      aacacattt actatttac tcatggaac attttactg 
      ttcttatgc agtattcca tcgcttgta agtgcgatt 
      atatttgtt ttcgtcccc gaaagccca aaatttctg 
      atgactatt ggcgaaaac gaaaaagct ttgaaaata 
      ttccagaaa atttatgcg aaaaacagc ggacaacca 
      ccaaagaca tttccaatt acggagttg gtagatgaa 
      gttagtctc aagaaaaaa gacccgaac gaacacgga 
      ggacaagtt actgctaaa cgaagtcag tcgcaagct 
      ttgcaggaa gggtggcaa caagtcaaa ccattattt 
      tttcctcct cacttaaaa aacataatt ttggtttgt 
      ctgattcaa actctgttc acaatgact gttaataca 
      atgaggctt tggttacca caaatattt caagctttg 
      aatgattac cagtattat tacaacgtg tcgtctgct 
      gatctttgc gaaagttta gaaatgatc aaaagttca 
      aatgttaca agtgatgaa tgccaagtc aattcggac 
      aattcttca gtttacata aacgctcta attgtagca 
      gttgttacc ctcgtgggt gatttaggt gcaagctct 
      cttatcaat aaagttggg aagaaaaca cttctattc 
      gttttagga atattagct ggaggattt gaaatcaca 
      atgtatttc tccatgaat acagcgttc gcagttgcg 
      ttttcatca ctgtttgct acttttaca cacattgca 
      gccaatgtt tgtattgta gtcattatt aatttattc 
      ccaacgact ttaaggaca atgtcatta gctttagct 
      ttaatgtgc ggcaggatt ggatcaatg gttggaaat 
      ttagtattt cctttactc attcggaca ggttgtggg 
      ccacctttt tttacaatt gccgcgcta gtttttgct 
      tgtgctttt ttgtccctg ctactacca aacaccgat 
      tccaaggcg ttggagtgt atcattatg tctggaaac 
      attttcacc gtcacaaca accaccaaa acgacagtt 
      ccagccgat tttgaaacc gctattacc gcaaccgat 
      ttcggaaaa tttaacttt tactttgtt ttgctaatc 
      cttgtttca ggatgggct tgcggcttt gaaagcagc 
      acaatggct tatattctt ccggtagcg cagtgtgat 
      cttgatttg agtcttgaa gacaaaggt ttattgaat 
      gcgattaca tattcggga atgatttca agtgctgtt 
      atttgggga tttctcttt gataccctt ggacgcaga 
      aaactccta atgatcggg ttttccttg gactttatc 
      tgcgtagtc ataagttcc ttatcccaa aacaaagaa 
      gtgttgcta gcgtcgaat ttttttggt ggttttatc 
      ataaatggc ccatatgcc gcactatca acttatgtt 
      tctgaattt cattcaaat aaatacaga ggacggatg 
      cagttaatg atagctgtg agtttttct tgcggaggc 
      atcgtctta ccccttctg gcttggggt attttacca 
      caagaatta aatttgaaa atatccgac acatttact 
      atttactcg tggaacatt ttacttttc ttgtatagt 
      attccatcg cttgtaagt gcgattata tttgttttc 
      gtcccagaa agcccaaaa tttctgatg actgtcggc 
      gaaaacgaa aaagctttg aaaatattc cagaaaatt 
      tattcaata aacagcgga caaccacca aagacattt 
      ccaattaca gagttggta gatgaagtt agtctcaag 
      aaaaaagac ccgaacgaa cacggagga caagttact 
      gctaaacga agtcagtcg caagctttg caggaaggg 
      tggcaacaa atcaaacca ttatttttt cctcctcac 
      ttaaaaaac ataattttt gtttgtctg attcaaact 
      ctgttcaca atgaccgtt aacacgatg agactttgg 
      ttaccacaa atattccaa gctttgaat gattaccaa 
      tattattac aacgtgtcg tctgctgat ctttgcgaa 
      agtttagaa atgatcaaa agttcaaat gttacaagt 
      gatgaatgc caagtcaat tcggacaat tcctcagtt 
      tacacaaac ggtttaatt gtagcagtt gttaccctt 
      gtaggtgat tttagtgca agctttttt atcaataaa 
      gttgggaaa aaaacactt ctcttcgtt ttaggaata 
      ttagctgga ggatttgaa atcaccatt tatttctcc 
      atgaataca gcgttcgca gttgcgttt tcatcactg 
      tttgctact tttacacac atcgcagcc aatgtttgt 
      attgtagtc attattaat ttattccca acgacttta 
      aggacaatg tcactagct ttagctcta atgtgtgct 
      agaattggt tcaatgact ggaaatcta atatttccc 
      ttactcatt cggacaggt tgtgggcca ccttttttc 
      acaatagca gcgctagtt tttgcttgt gcttttttg 
      tccctgtta ctaccaaac accgactcc aaggcgctg 
      gaataa
    mRNA sequence
    • >TC032058 RA
      tttcgataa cagtagcac tgtttaaac gaagctata 
      aaaattcaa cgacctgca ttttgaagg tgtgtcaca 
      atgtctgga aacattttc acagttaca acaaacacc 
      aaaatgaca gttccagcc gattttgaa accgctatt 
      acagcaacc ggtttcggc aaatttaat tttttattt 
      cttttgcta atccttgcg tcaggatgg gctgcaact 
      ttggaaagt agcacaatg gcttatgtt tttccagca 
      gcacagtgt gaccttgat ttgagtctt gaagataaa 
      ggattattg aatgctatt acatactct ggaatgatt 
      tcaagtgct gttatttgg ggattcctc tttgatacc 
      cttggacgc agaaaactt ctaatgatt gggtttttc 
      ctggacttt atttgcgta gtcataagt tccttatcc 
      caaaacaaa gaagtgttg atagcatcg aaattcttt 
      ggtggcttt attatgaat ggcccttat gccgctcta 
      tcaacttat gtttctgaa tttcactca aataattat 
      agaggacga atgcaatta atgatagca atgagtttt 
      tgttgcgga ggcatcgtc ttacccctt ctagcttgg 
      ggtattttg ccacaagaa ttaaatttg caaatatcc 
      aacacattt actatttac tcatggaac attttactg 
      ttcttatgc agtattcca tcgcttgta agtgcgatt 
      atatttgtt ttcgtcccc gaaagccca aaatttctg 
      atgactatt ggcgaaaac gaaaaagct ttgaaaata 
      ttccagaaa atttatgcg aaaaacagc ggacaacca 
      ccaaagaca tttccaatt acggagttg gtagatgaa 
      gttagtctc aagaaaaaa gacccgaac gaacacgga 
      ggacaagtt actgctaaa cgaagtcag tcgcaagct 
      ttgcaggaa gggtggcaa caagtcaaa ccattattt 
      tttcctcct cacttaaaa aacataatt ttggtttgt 
      ctgattcaa actctgttc acaatgact gttaataca 
      atgaggctt tggttacca caaatattt caagctttg 
      aatgattac cagtattat tacaacgtg tcgtctgct 
      gatctttgc gaaagttta gaaatgatc aaaagttca 
      aatgttaca agtgatgaa tgccaagtc aattcggac 
      aattcttca gtttacata aacgctcta attgtagca 
      gttgttacc ctcgtgggt gatttaggt gcaagctct 
      cttatcaat aaagttggg aagaaaaca cttctattc 
      gttttagga atattagct ggaggattt gaaatcaca 
      atgtatttc tccatgaat acagcgttc gcagttgcg 
      ttttcatca ctgtttgct acttttaca cacattgca 
      gccaatgtt tgtattgta gtcattatt aatttattc 
      ccaacgact ttaaggaca atgtcatta gctttagct 
      ttaatgtgc ggcaggatt ggatcaatg gttggaaat 
      ttagtattt cctttactc attcggaca ggttgtggg 
      ccacctttt tttacaatt gccgcgcta gtttttgct 
      tgtgctttt ttgtccctg ctactacca aacaccgat 
      tccaaggcg ttggagtgt atcattatg tctggaaac 
      attttcacc gtcacaaca accaccaaa acgacagtt 
      ccagccgat tttgaaacc gctattacc gcaaccgat 
      ttcggaaaa tttaacttt tactttgtt ttgctaatc 
      cttgtttca ggatgggct tgcggcttt gaaagcagc 
      acaatggct tatattctt ccggtagcg cagtgtgat 
      cttgatttg agtcttgaa gacaaaggt ttattgaat 
      gcgattaca tattcggga atgatttca agtgctgtt 
      atttgggga tttctcttt gataccctt ggacgcaga 
      aaactccta atgatcggg ttttccttg gactttatc 
      tgcgtagtc ataagttcc ttatcccaa aacaaagaa 
      gtgttgcta gcgtcgaat ttttttggt ggttttatc 
      ataaatggc ccatatgcc gcactatca acttatgtt 
      tctgaattt cattcaaat aaatacaga ggacggatg 
      cagttaatg atagctgtg agtttttct tgcggaggc 
      atcgtctta ccccttctg gcttggggt attttacca 
      caagaatta aatttgaaa atatccgac acatttact 
      atttactcg tggaacatt ttacttttc ttgtatagt 
      attccatcg cttgtaagt gcgattata tttgttttc 
      gtcccagaa agcccaaaa tttctgatg actgtcggc 
      gaaaacgaa aaagctttg aaaatattc cagaaaatt 
      tattcaata aacagcgga caaccacca aagacattt 
      ccaattaca gagttggta gatgaagtt agtctcaag 
      aaaaaagac ccgaacgaa cacggagga caagttact 
      gctaaacga agtcagtcg caagctttg caggaaggg 
      tggcaacaa atcaaacca ttatttttt cctcctcac 
      ttaaaaaac ataattttt gtttgtctg attcaaact 
      ctgttcaca atgaccgtt aacacgatg agactttgg 
      ttaccacaa atattccaa gctttgaat gattaccaa 
      tattattac aacgtgtcg tctgctgat ctttgcgaa 
      agtttagaa atgatcaaa agttcaaat gttacaagt 
      gatgaatgc caagtcaat tcggacaat tcctcagtt 
      tacacaaac ggtttaatt gtagcagtt gttaccctt 
      gtaggtgat tttagtgca agctttttt atcaataaa 
      gttgggaaa aaaacactt ctcttcgtt ttaggaata 
      ttagctgga ggatttgaa atcaccatt tatttctcc 
      atgaataca gcgttcgca gttgcgttt tcatcactg 
      tttgctact tttacacac atcgcagcc aatgtttgt 
      attgtagtc attattaat ttattccca acgacttta 
      aggacaatg tcactagct ttagctcta atgtgtgct 
      agaattggt tcaatgact ggaaatcta atatttccc 
      ttactcatt cggacaggt tgtgggcca ccttttttc 
      acaatagca gcgctagtt tttgcttgt gcttttttg 
      tccctgtta ctaccaaac accgactcc aaggcgctg 
      gaataaact ttggaatga tgtttcaat tttattgtg 
      taaaaattt tgtaaatac cattcctgt tataaaaag 
      taaaagaaa gttttctac gttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY